• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.56 no.4
• /
• pp.349-360
• /
• 2011

The objectives of present study were to characterize the peptides which were isolated from Korean fermented soybean paste, chungkukjang, and to determine their antioxidant activities. Four fractions were collected from the methanol extract of chungkukjang by using a recycling preparative HPLC. Among fractions, Fr-2 was identified to be highly potent free radical scavenging activity in the assay of 1,1-diphenyl-2-picryl-hydrazyl (DPPH) and nitroblue tetrazolium(NBT)-reduction inhibition. Base on antioxidant effects, fraction Fr-2 was employed for the refraction with a prep-column and separated into five fractions of which two fractions were identified to have higher antioxidant activity. To confirm the amino acid constituents of antioxidant fractions Fr-2-2 and Fr-2-3 were analyzed, and eight kinds of amino acids such as aspartic acid, threonine, serine, glutamic acid, glycine, lysine, histidine, and arginine were identified as the constituent amino acids. Antioxidant activities of the separated peptides were further assessed cell viability with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl terazolium bromide (MTT), and fluorescence-activated cell sorting (FACS) analysis of H4IIE cells treated with hydrogen peroxide (H2O2). Chungkukjang peptides have shown their ability to protect H4IIE rat hepatoma cells against H2O2- induced oxidative stress by concentration and time-dependent manner. Therefore, These results indicated that fermented soybean paste chungkukjang will be promoted the antioxidant and radical scavenging activities, and beneficial for health. The antioxidant peptide fractions Fr-2-2 and Fr-2-3 were denominated as P-NICS-1 and P-NICS-2, respectively. However, further studies were required to clarify their amino acid sequences and molecular properties, and physiological significances.

• Journal of Microbiology and Biotechnology
• /
• v.15 no.6
• /
• pp.1402-1407
• /
• 2005

The fungicidal activities of Coptis japonica (Makino) extracts and their active principles were determined against Botrytis cineria, Erysiphe graminis, Phytophthora infestans, Puccinia recondita, Pyricularia grisea, and Rhizoctonia solani using a whole plant method in vivo, and compared with natural fungicides. The responses varied according to the plant pathogen tested. At 2,000 mg/l, the chloroform and butanol fractions obtained from methanolic extracts of C. japonica exhibited strong/moderate fungicidal activities against B. cinerea, E. graminis, P. recondita, and Py. grisea. Two active constituents from the chloroform fractions and one active constituent from the butanol fractions were characterized as isoquinoline alkaloids, berberine chloride, palmatine iodide, and coptisine chloride, respectively, using spectral analysis. Berberine chloride had an apparent $LC_{50}$ value of approximately 190, 80, and 50 mg/l against B. cinerea, E. graminis, and P. recondita, respectively; coptisine chloride had an $LC_{50}$ value of 210,20, 180, and 290 mg/l against B. cinerea, E. graminis, P. recondita, and Py. grisea, respectively; and palmatine iodide had an $LC_{50}$ value of 160 mg/l against Py. grisea. The isoquinoline alkaloids were also found to be more potent than the natural fungicides, curcumin and emodin. Therefore, these compounds isolated from C. japonica may be useful leads for the development of new types of natural fungicides for controlling B. cinerea, E. graminis, P. recondita, and Py. grisea in crops.

• Journal of Microbiology and Biotechnology
• /
• v.30 no.6
• /
• pp.893-902
• /
• 2020

Propolis is a resinous substance that is collected by Apis mellifera from plant sources and is used in traditional medicine. To study the phytochemical constituents and apoptotic potential of Jordanian propolis extract against different cancer cell lines, propolis was extracted using methanol, hexane, and ethyl acetate and was fractionated using chromatographic methods. Cytotoxicity was assessed using MTT and LDH assays. The apoptotic potential was investigated using florescence microscopy, multicaspase assay, Annexin-V and dead cell assay, and cell cycle assay. The phytochemical constituents were analyzed using GC-MS. The methanol extract of propolis exhibited cytotoxic potential against all cell lines tested. The IC₅₀ values of the methanol extract were 47.4, 77.8, 91.2, and 145.0 ㎍/ml for HepG2, LoVo, MDAMB231, and MCF7 cell lines, respectively. The IC₅₀ values of the F1 fraction were 31.6 (MDAMB231), 38.9 (HepG2), 36.7 (LoVo) and 75.5 (MCF7) ㎍/ml. On further purification using thin-layer chromatography, the IC₅₀ values of the F1-3 fraction were found to be 84.31(HepG2), 79.2 (MCF7), 70.4 (LoVo), and 68.9 (MDAMB231) ㎍/ml, respectively. The anticancer potential of the F1 fraction was confirmed through the induction of apoptosis and cell cycle arrest at the G0/G1 phase. The GC-MS analysis of the F1 fraction revealed the presence of 3-methyl-4-isopropylphenol (29.44%) as a major constituent. These findings indicate the potential of propolis extract as a cancer therapy. However, further investigation is required to assess the acute and subacute toxicity of the most active fraction.

• The Journal of the Korea Contents Association
• /
• v.17 no.7
• /
• pp.455-477
• /
• 2017

The purpose of this study is to develop the service scales of festival quality. We derived five constituent factors, service quality measures of local festivals through the extensive literature review in Previous studies. We named these qualities "COKITQUAL" that is consist of Convenience, Information, Kindness, Operation, Corporeity. We conducted the empirical analysis for the participants in "Hwacheon Sancheoneo Festival" to verify the "COKITQUAL". As the result, Information, Kindness, Operation affected positively on Festival Satisfaction among all SERVQUALs. However, Convenience and Corporeity did not positively affected on the dependent variable. And then, Festival Satisfaction positively affected on on Behavior Intention. This study developed the Service quality concerned with local festivals which is crucial fundamentals to quantify Festival SERVQUAL through empirical study. The proper applications and usage of these newly introduced local festival qualities enable to acquire the sustainable competitive advantage in local festival.

• The Journal of the Korea Contents Association
• /
• v.11 no.12
• /
• pp.475-485
• /
• 2011

The purpose of this study is to find intervention strategies of university and social welfare agency through analysis of factors influencing on field education outcomes and professional identity of social welfare in cyber university and ordinary university students. The major findings are as follows. First, the degree of field education outcome in cyber university students is higher than ordinary university ones. Second, supervision and university element are meaningful factors to constituent variables of field education outcomes in participants, but major satisfaction is only important for ordinary university students. Third, professional identity is related to major satisfaction, supervision, and university element in cyber university students. But university element hasn't influence on professional identity in ordinary university students. Therefore, supervisors have to give systematic instruction and do clear role model. Universities make an effort to improve major satisfaction and the quality as a pre-social worker.

• Journal of Microbiology and Biotechnology
• /
• v.28 no.5
• /
• pp.679-687
• /
• 2018

Korean red pine (Pinus densiflora) is one of the major Pinus species in Korea. Red pine bark is removed prior to the chipping process in the wood industry and discarded as waste. However, red pine bark contains a considerable amount of naturally occurring phenolics, including flavonoids, and therefore may have a variety of biological effects. In this study, we investigated if Korean red pine bark extract (KRPBE) could protect neuronal PC-12 cells from oxidative stress and inhibit cholinesterase activity. Analysis of reversed-phase high-performance liquid chromatography results revealed four phenolics in KRPBE: vanillin, protocatechuic acid, catechin, and taxifolin. The total phenolic and flavonoid contents of KRPBE were 397.9 mg gallic acid equivalents/g dry weight (DW) and 248.7 mg catechin equivalents/g DW, respectively. The antioxidant capacities of KRPBE measured using ABTS, DPPH, and ORAC assays were 697.3, 521.8, and 2,627.7 mg vitamin C equivalents/g DW, respectively. KRPBE and its identified phenolics protected against $H_2O_2$-induced oxidative cell death in a dose-dependent manner. Acetylcholinesterase and butyrylcholinesterase, which degrade the neurotransmitter acetylcholine to terminate neurotransmission in synaptic clefts, were inhibited by treatment with KRPBE and its identified phenolics. Taken together, these results suggest that KRPBE and its constituent antioxidative phenolics are potent neuroprotective agents that can maintain cell viability under oxidative stress and inhibit cholinesterase activity.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
• /
• v.5 no.2
• /
• pp.145-154
• /
• 2007

In hydrothermal reaction tests, smectite-to-illite conversion was identified using a domestic bentonite which is favorably considered as a buffer material, and its dependency on various hydrothermal conditions was investigated. The analysis results of the XRD and Si concentration indicated that the smectite-to-illite conversion was a major process of bentonite alteration under the hydrothermal conditions. The temperature, potassium concentration in solution, and pH were observed to significantly affect the smectite-to illite conversion. A model of conversion reaction rate was suggested to evaluate the long-term stability of smectite composing a major constituent of bentonitic buffer. It was expected from the evaluation results that the smectite would keep its integrity for very long disposal time under a normal condition, whitens it might be converted to illite by 50 percent after over $5{\times}10^4$ year of disposal time under a conservative condition and consequently lose its swelling capacity as a buffer material of a repository.

• Magazine of the Korean Society of Agricultural Engineers
• /
• v.44 no.5
• /
• pp.116-126
• /
• 2002

Discharge pattern and water quality were investigated in the drainage water from about 10 ha of groundwater-irrigated paddy field in the growing season of 2001. Total discharge quantity was about 1,117.2 mm in which about 75% was caused by management drainage due to cultural practice of paddy rice farming and the rest by rainfall runoff where total rainfall was about 515 mm. Dry-day sampling data showed wide variations in constituent concentrations with average of 26.14 mg/L, 0.37 mg/L, 3.54 mg/L at the inlet, and 43.60 mg/L, 0.34 mg/L, 3.58 mg/L at the outlet for CO $D_{cr}$ , T-P, and T-N, respectively. Wet-day sampling data demonstrated that generally CO $D_{cr}$ followed the discharge pattern and T-P was in opposite to the discharge pattern, but T-N did not show apparent pattern to the discharge. Discharge and load are in strong relationship. And based on regression equation, pollutant loads from groundwater irrigation area are estimated to be 288.34, 1.17, and 5.45 kg/ha for CO $D_{cr}$ , T-P, and T-N, respectively, which was relatively lower than the literature value from surface water irrigation area which implies that groundwater irrigation area might use less irrigation water and result in less drainage water, Therefore, total pollutant load from paddies irrigation with groundwater could be significantly lower than that with surface water. This study shows that agricultural drainage water management needs a good care of drainage outlet as well as rainfall runoff. This study was based on limited monitoring data of one year, and further monitoring and successive analysis are recommended for more generalized conclusion.

• Steel and Composite Structures
• /
• v.18 no.6
• /
• pp.1493-1515
• /
• 2015

A new refined hyperbolic shear deformation theory (RHSDT), which involves only four unknown functions as against five in case of other shear deformation theories, is presented for the thermoelastic bending analysis of functionally graded sandwich plates. Unlike any other theory, the number of unknown functions involved is only four, as against five in case of other shear deformation theories. The theory presented is variationally consistent, does not require shear correction factor, and gives rise to transverse shear stress variation such that the transverse shear stresses vary parabolically across the thickness satisfying shear stress free surface conditions. The sandwich plate faces are assumed to have isotropic, two-constituent material distribution through the thickness, and the modulus of elasticity, Poisson's ratio of the faces, and thermal expansion coefficients are assumed to vary according to a power law distribution in terms of the volume fractions of the constituents. The core layer is still homogeneous and made of an isotropic ceramic material. Several kinds of sandwich plates are used taking into account the symmetry of the plate and the thickness of each layer. The influences played by the transverse shear deformation, thermal load, plate aspect ratio and volume fraction distribution are studied. Numerical results for deflections and stresses of functionally graded metal-ceramic plates are investigated. It can be concluded that the proposed theory is accurate and simple in solving the thermoelastic bending behavior of functionally graded plates.

• Journal of Powder Materials
• /
• v.18 no.6
• /
• pp.532-537
• /
• 2011

Thermally stable $TiO_2$/Pt/$SiO_2$ core-shell nanocatalyst has been synthesized by chemical processes. Citrated capped Pt nanoparticles were deposited on amine functionalized silica produced by Stober process. Ultrathin layer of titania was coated on Pt/$SiO_2$ for preventing sintering of the metal nanoparticles at high temperatures. Thermal stability of the metal-oxide hybrid catalyst was demonstrated heating the sample up to $600^{\circ}C$ in air and by investigating the morphology and integrity of the structure by transmission electron spectroscopy. The surface analysis of the constituent elements was performed by X-ray photoemission spectroscopy. The catalytic activity of the hybrid catalysts was investigated by CO oxidation reaction with oxygen as a model reaction.