• Title/Summary/Keyword: Congo red

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Degradation of Chlorinated Phenolic Compounds by Soil Actinomycetes Isolated from the Contami-nated Soil Nearby the Kyung-An River (경안천 유역 오염토양에서 분리한 방선균의 염화 페놀계 화합물 분해)

  • 김성민;김창영;김응수
    • Microbiology and Biotechnology Letters
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    • v.30 no.3
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    • pp.287-292
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    • 2002
  • Lignin-peroxidase (LiP) has been considered as one of the most important industrial enzymes for biodegradation of various recalcitrant toxic compounds such as chlorinated aromatic hydrocarbons and azo-dyes. Recently, several soil actinomycetes have been reported to secrete a functionally-similar lignin-peroxidase called actinomycetes lig-nin-peroxidase (ALiP). In this manuscript, we isolated over 100 morphologically distinct actinomycetes from the contaminated soils around 10 different gas stations located nearby the Kyung-An river. Among these actinomycetes screened based on the congo-red dye-decolorization activities, one newly-isolated actinomycetes named SMA-2 showed the most significant dye-decoloring activity on the congo-red plate as well as a significant ALiP activity in a yeast-extract-malt-extract liquid media supplemented with starch. The optimum SMA-2 culture condition fur ALiP production was determined and the kinetic parameters fur the SMA-2 AkIP activity were characterized. The optimally-cultured SMA-2 also exhibited the oxidation activities toward various recalcitrant aromatic compounds including phenol, 2- chlorophenol, 4- chlorophenol, 2,4- dichlorophenol ,2,6- dichlorophenol, and 2,4, f-trichlorophe - not, suggesting a potential application of SMA-2 for contaminated soil bioremediation.

Equilibrium, kinetic and thermodynamic studies of the adsorption of acidic dye onto bagasse fly ash

  • Shouman, Mona A.;Fathy, Nady A.;El-Khouly, Sahar M.;Attia, Amina A.
    • Carbon letters
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    • v.12 no.3
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    • pp.143-151
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    • 2011
  • Bagasse fly ash (BFA) is one of the important wastes generated in the sugar industry; it has been studied as a prospective low-cost adsorbent in the removal of congo red (CR) from aqueous solutions. Chemical treatment with $H_2O_2$ was applied in order to modify the adsorbability of the raw BFA. Batch studies were performed to evaluate the influence of various experimental parameters such as dye solution pH, contact time, adsorbent dose, and temperature. Both the adsorbents were characterized by Fourier-transform infrared spectrometer, energy-dispersive X-ray spectrophotometer and nitrogen adsorption at 77 K. Equilibrium isotherms for the adsorption of CR were analyzed by Langmuir, Freundlich and Temkin models using non-linear regression technique. Intraparticle diffusion seems to control the CR removal process. The obtained experimental data can be well described by Langmuir and also followed second order kinetic models. The calculated thermodynamic parameters indicate the feasibility of the adsorption process for the studied adsorbents. The results indicate that BFA can be efficiently used for the treatment of waste water containing dyes.

Elimination of COD and Color of Dye by UV/H2O2, UV/TiO2 System (UV/H2O2, UV/TiO2 시스템에서 염료의 색도 및 COD 제거)

  • Kim, Kei-Woul;Park, Joung Mi;Sim, Su-Jin;Yee, Hi-Joung;Rhee, Dong Seok
    • Journal of Industrial Technology
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    • v.20 no.A
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    • pp.51-56
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    • 2000
  • The Photocatalytic decolorization and degradation of commercial dyes were studied using a batch reactor. Degussa P25 titanium dioxide and $H_2O_2$ were used as the photocatalyst and proved to be effective for dyes degradation when they were irradiated with UV light. The light source was a 20W low pressure mercury lamp. Three different kinds of dyes, such as direct dye(congo red), acid dye (acid black) and disperse dye(disperse blue) were tested. Extending the UV only treatment up to 120min, direct dye was decolorized to 60% and degraded to 30% as COD. On the other side, acid and disperse dyes were eliminated less than 10% as color and COD. But, color and COD were eliminated about 90% for all of the three dyes by $UV/H_2O_2$ system. And then the most effective decolorization was done for direct dye with 96% removal efficiency by $UV/TiO_2$ system at 120min with 500mg/L of $TiO_2$.

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Trametes sp. CJ-105에 의한 염료의 색도제거

  • Kim, Hyun-Soo;Oh, Kwang-Keun;Lee, Cheol-Woo;Lee, Jae-Heung;Jeon, Yeong-Joong
    • Microbiology and Biotechnology Letters
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    • v.25 no.6
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    • pp.630-635
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    • 1997
  • Decolorization of congo red, methyl orange, poly R478, remazol brilliant blue R and crystal violet by white-rot fungus Trametes sp. CJ-105, isolated in Korea, was investigated. Remazol blue and methyl orange were almost completely decolorized after 2 days of culture, but congo red, crystal violet and poly R478 were decolorized by about 80%, 40% and 30% after 10 days of culture, respectively. As a result of determination of cell mass and enzyme activity, it was shown that color removal efficiency was related to cell mass and enzyme activity, and also found that only laccase (E.C.1.10.3.2) activity was existed in the culture broth. The decolorization ratios of remazol blue in the concentrations of 100ppm to 3, 000 ppm were 85% and above after 2 days of culture. In this study, we found that white-rot fungus, Trametes sp. CJ-105, was effective in decolorizing a wide range of structurally different synthetic dyes.

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Structural Characteristics of Immunostimulating Polysaccharides from Lentinus edodes

  • Lee, Hee-Hwan;Lee, Jong-Seok;Cho, Jae-Yeol;Kim, Young-Eon;Hong, Eock-Kee
    • Journal of Microbiology and Biotechnology
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    • v.19 no.5
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    • pp.455-461
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    • 2009
  • There is a significant amount of experimental evidence suggesting that polysaccharides from mushrooms enhance the host immune system by activating various mechanisms in immune cells, including macrophages. In this study, polysaccharides from Lentinus edodes were found to stimulate the functional activation of macrophages to secrete inflammatory mediators and cytokines and increase the phagocytotic uptake. The chemical properties of the stimulatory polysaccharides, CPFN-G-I, CPBN-G, and CPBA-G, were determined based on their monosaccharide composition, which mainly consisted of glucose and mannose. According to FT-IR and GC/MS, the structure of CPFN-G-I, purified from the fruiting body of L. edodes, was found to consist of a $\beta$-1,6-branched-$\beta$-1,4-glucan, whereas CPBN-G and CPBA-G, purified from the liquid culture broth, were found to be composed of a heteromannan. The configuration of the p-linkage and triple helical conformation of each polysaccharide were confirmed using a Fungi-Fluor kit and Congo red, respectively.

Studies on the Enterobacteriaceae(Salmonella, Shigella and E. coli) Isolated in Korea -The Pathogenic Characters of Shigella flexneri in vivo and in vitro Isolated from Korea, 1986- (한국에서 분리된 장내세균(Salmonella, Shigella, E. coli 균속)의 병원적 역할에 관한 연구(II) -Shigella flexneri의 병원성에 관한 연구-)

  • Kim, Ki-Sang;Yoo, Cheon-Kwon;Sohn, Kun-Young;Lee, Bok-Kwon;Lee, Myung-Won;Lee, Yun-Tai;Jung, Tae-Hwoa
    • The Journal of the Korean Society for Microbiology
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    • v.22 no.1
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    • pp.71-78
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    • 1987
  • In order to determine the virulence properties of eleven strains of Sh. flexneri isolated from diarrheal patients the congo red test, the $Ser{\acute{e}}ny$ test, the HeLa cell invasion test and electrophoresis of plasmids were carried out. The results were summarized as follows. 1. Virulent strains were not determined by the result of Congo red absorption test. 2. Virulent strains showed positive reaction by the Sereny test and the HeLa cells invasion, but avirulent strains revealed negative reaction at those tests. 3. The temperature condition of bacterial growth was a factor of virulent expression. 4. Virulent strains were mostly possessed of a 130.3 Mdal plasmid, but avirulent strains were not.

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Decolorization of Aromatic Dyes by White Rot Fungus Coriolus hirsutus (흰구름버섯(Coriolus hirsutus)에 의한 방향족 염료의 탈색)

  • Song, Yeon-Hong;Choi, Chul-Min;Kim, Chang-Jin;Shin, Kwang-Soo
    • Korean Journal of Microbiology
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    • v.33 no.4
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    • pp.252-256
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    • 1997
  • The white rot fungus Coriolus hiysutus decolorized several recalcitrant dyes. Four different types of dyes, including azo, triphenyl methane, heterocyclic, and polymeric dye, were treated by the mycelial preparation. Triphenyl methane dye, bromophenol blue lost over 95% of its color. Congo red and Poly R-478 were decolorized less than bromophenol blue, 57 and 55%, respectively. However, heterocyclic dye, methylene blue was not decolorized significantly and only red shift was observed. Extracellular laccase and peroxidase activities were appeared maximally in high level of dye decolorization media. In electrophoretic experiments, common active bands of laccase and peroxidase were found in all dye decolorized medium. These results indicated that the culture conditions which yield high levels of laccase and peroxidase activity lead to high levels of dye decolorization, and these two enzymes might be play an important roles in dye decolorization.

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Pulmonarg Nodular Amyloidosis (폐실질내 결절형 유전분증 - 수술치험 1례 -)

  • 전도환;이충석;조성래;김영옥
    • Journal of Chest Surgery
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    • v.32 no.11
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    • pp.1060-1063
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    • 1999
  • Amyloidosis is a rare disease which is characterized by the deposition of a histochemically specific substance called amyloid in many tissue bodies, and causes various symptoms according to the organs involved. Amyloid is usually recognized by its staining reaction with Congo red stain. Primary pulmonary amyloidosis is very rare. Nodular pulmonary amyloidosis is an uncommon entity that usually manifests itself as an asymptomatic incidental finding on the chest roentgenogram and is misdiagnosed as lung cancer or pulmonary tuberculosis.

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