Studies on the Enterobacteriaceae(Salmonella, Shigella and E. coli) Isolated in Korea -The Pathogenic Characters of Shigella flexneri in vivo and in vitro Isolated from Korea, 1986-

한국에서 분리된 장내세균(Salmonella, Shigella, E. coli 균속)의 병원적 역할에 관한 연구(II) -Shigella flexneri의 병원성에 관한 연구-

In order to determine the virulence properties of eleven strains of Sh. flexneri isolated from diarrheal patients the congo red test, the $Ser{\acute{e}}ny$ test, the HeLa cell invasion test and electrophoresis of plasmids were carried out. The results were summarized as follows. 1. Virulent strains were not determined by the result of Congo red absorption test. 2. Virulent strains showed positive reaction by the Sereny test and the HeLa cells invasion, but avirulent strains revealed negative reaction at those tests. 3. The temperature condition of bacterial growth was a factor of virulent expression. 4. Virulent strains were mostly possessed of a 130.3 Mdal plasmid, but avirulent strains were not.