• Title/Summary/Keyword: Confocal microscopy

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MR 분자영상용 후보물질의 탐색: 예비연구

  • 박지애;장용민;강봉석;이종민;강덕식
    • Proceedings of the KSMRM Conference
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    • 2003.10a
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    • pp.36-36
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    • 2003
  • 목적: 고형성 종양에 특이적으로 발현하는 MR 분자영상용 조영제의 후보물질을 분자생물학적 방법 및 in vitro 분자영상기법을 이용하여 탐색하고자 하였다. 대상 및 방법: 암이 진행됨에 따라 유전자가 과발현되는 세포표면 단백질인 CD44 와 세포막에 존재하는 단백질분해효소의 일종인 MTI-MMP를 일차 선정하여 유전자 수준 및 단백질 수준에서 분자생물학적 방법으로 종양특이성에 대해 검증하였고, 특히 각종 암세포에서 발현양이 증가하는 CD44의 변형인 CD44v6 항체를 이용하여 생세포에서의 표적화에 대한 광학적 검증을 위해 초상자성 나노입자 대신 형광입자(FITC)를 결합하여 confocal microscopy로 실시간 표적화에 대한 분자영상을 획득하였다.

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Ultrastructural observations of vegetative cells of two new genera in the Erythropeltidales (Compsopogonophyceae, Rhodophyta): Pseudoerythrocladia and Madagascaria

  • Scott, Joseph L.;Orlova, Evguenia;West, John A.
    • ALGAE
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    • v.25 no.1
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    • pp.11-15
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    • 2010
  • Two new genera of red algae, Madagascaria erythrocladioides West et Zuccarello and Pseudoerythrocladia kornmannii West et Kikuchi (Erythropeltidales, Compsopogonophyceae, Rhodophyta), were previously described using molecular analysis and confocal microscopy of isolates in laboratory culture. We examined the ultrastructure of both genera to compare with ultrastructure of other members of the class Compsopogonophyceae. Both genera had Golgi bodies not associated with mitochondria and chloroplasts with a peripheral encircling thylakoid similar to all other members of the class studied thus far. Confocal autofluorescence images showed that Madagascaria has a single round central pyrenoid while Pseudoerythrocladia has no pyrenoid. Our electron microscopic work confirms these initial observations. Tables and keys are presented that assist in interpreting cellular details of genera in the class Compsopogonophyceae.

The Wear Mechanism of Carbon(WC/C) Thin Film in Lubricated Contact (WC/C 박막의 윤활접촉하의 마멸기구)

  • Ahn, Hyo-Sok;Kim, Doo-In
    • Tribology and Lubricants
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    • v.24 no.6
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    • pp.302-307
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    • 2008
  • The running-in behaviour of the metal carbon coating (WC/C) was evaluated with regard to its applicability as wear-resistant coating for key components in engines. Reciprocating wear tests under lubricated condition employing an oscillating friction wear tester were performed to investigate the tribological behaviors of the coatings in ambient environment. Confocal microscopy and scanning electron microscopy were used to observe worn surfaces and the wear scars on the steel balls. Elemental composition of the coating and worn surfaces were characterized using Auger electron spectroscopy. The friction behavior of WC/C coating was comparable to common carbon-based coatings. Thin tribofilm was formed on the worn surface of the steel ball due to material transfer and tribochemical reaction whereas there was no evidence of tribofilm generation on the coating surface. indicating the chemical innertness of WC/C coating.

Pharmacognostical Evaluation of Clerodendrum viscosum (Vent.)

  • Lobo, Richard;Kumar, C. Dinesh;Punitha, I.S.R.;Rajendran, K.;Shirwaikar, Arun;Shirwaikar, Annie
    • Natural Product Sciences
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    • v.12 no.2
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    • pp.79-84
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    • 2006
  • This study presents a detailed pharmacognostical study of the root and leaf of the crude drug Clerodendrum viscosum Vent. (Verbenaceae), an important plant in the Indian system of medicine. The root and leaf samples were studied using light and confocal microscopy, WHO recommended-physicochemical determinations, and authentic phytochemical procedures. The physicochemical, morphological, and histological parameters presented in this paper may be proposed as parameters to establish the authenticity of C. viscosum root and leaf, and may possibly help to differentiate the drug from its other species.

Histological and Physico-Chemical Evaluation of Hybanthus enneaspermus (L.) F. Muell

  • Narayanaswamy, V.B.;Kumar, C. Dinesh;Setty, M. Manjunath;Shirwaikar, Annie
    • Natural Product Sciences
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    • v.12 no.2
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    • pp.104-108
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    • 2006
  • This paper presents a detailed pharmacognostical study of the plant drug Hybanthus enneaspermus (L.) F. Muell (Violaceae), an important drug in the Indian system of medicine. The leaf and stem samples were studied using procedures of light, confocal microscopy, WHO recommended physico-chemical determinations and authentic phytochemical procedures. The physico-chemical, morphological, and histological parameters presented in this paper may be proposed as parameters to establish the authenticity of H. enneaspermus and may possibly help to differentiate the drug from its adulterants.

Effects of Proto-oncogene Protein DEK on PCAF Localization

  • Lee, In-Seon;Lee, Seok-Cheol;Lee, Jae-Hwi;Seo, Sang-Beom
    • Biomolecules & Therapeutics
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    • v.15 no.2
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    • pp.78-82
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    • 2007
  • The proto-oncogene protein DEK is a nuclear binding phosphoprotein that has been associated with various human diseases including leukemia. Histone acetylation is an important post-translational modification which plays important role in transcriptional regulation. Auto-acetylation of histone acetyltransferase PCAF results in increment of its HAT activity and facilitation of its nuclear localization. In this study, we report that DEK inhibits PCAF auto-acetylation through direct interaction. The C-terminal acidic domains of DEK are responsible for the interaction with PCAF. Using confocal microscopy, we have shown that nuclear localization of PCAF is severely inhibited by DEK. Taken together, our results suggest that DEK may be involved in various cellular signal transduction pathways accommodated by PCAF through the regulation of PCAF auto-acetylation.

An Identity Based Pharmacognostical Profile of folium Annona squamosa

  • Kumar, C. Dinesh;Rajendran, K.;Lobo, Richard;Shirwaikar, Annie
    • Natural Product Sciences
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    • v.11 no.4
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    • pp.213-219
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    • 2005
  • The leaves of Annona squamosa Linn. (Annonaceae), commonly called as custard apple, are popularly used for the treatment of diabetes by various tribes in India. The leaves are also used for their powerful insecticidal activity, especially for destroying lice. This paper present a detailed pharmacognostical study of the crude drug folium Annona squamosa. The samples were studied using procedures of light, confocal microscopy, WHO recommended physico-chemical determinations and authentic phytochemical procedures. The physicochemical, morphological and histological parameters presented in this paper may be proposed as parameters to establish the authenticity of Annona squamosa leaf and may possibly help to differentiate the drug from its adulterants.

In Vitro Cellular Uptake and Cytotoxicity of Paclitaxel-Loaded Glycol Chitosan Self-Assembled Nanoparticles

  • Park, Ji-Sun;Cho, Yong-Woo
    • Macromolecular Research
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    • v.15 no.6
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    • pp.513-519
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    • 2007
  • Self-assembled nanoparticles have great potential to act as vehicles for hydrophobic drug delivery. Understanding nanoparticle cellular internalization is essential for designing drugs intended for intracellular delivery. Here, the endocytosis and exocytosis of fluorescein isothiocyanate (FITC)-conjugated glycol chitosan (FGC) self-assembled nanoparticles were investigated by flow cytometry and confocal microscopy. The cellular internalization of FGC nanoparticles was initiated by nonspecific interactions between nanoparticles and cell membranes. Although adsorptive endocytosis of the nanoparticles occurred quickly, significant amounts of FGC nanoparticles were exocytosed, particularly in the early stage of endocytosis. The amount of exocytosed nanoparticles was dependent on the pre-incubation time with nanoparticles, suggesting that exocytosis is dependent on the progress of endocytosis. FGC nanoparticles internalized by adsorptive endocytosis were distributed in the cytoplasm, but not in the nucleus. In vitro cell cycle analysis demonstrated that FGC nanoparticles delivered paclitaxel into the cytoplasm and were effective in arresting cancer cell growth.

The study of property on the michelson interferometric microscopy (마이켈슨 간섭계형 현미경의 특성 연구)

  • 김경신;권남익
    • Korean Journal of Optics and Photonics
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    • v.10 no.5
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    • pp.369-372
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    • 1999
  • We have constructed an interferometric microscopy using a Michelson interferometer and a He-Ne laser. The three dimensional image was obtained by the interference from the reflected signal by a sample surface and from the reflected signal by a mirror. The axial resolution obtained by Michelson interferometric microscopy is as good as that of the white-light interferometer, but the same fringe is obtained when optical path difference is half-wavelength. The image from Michelson interferometric microscopy was compared with the images from the various types of confocal microscopy.

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Display of green fluorescent protein (GFP) on the cell surface of Zymomonas mobilis using N-terminal domain of ice nucleation protein (빙핵활성단백질의 N-terminal 부분을 이용한 녹색형광단백질의 Zymomonas mobilis 세포 표면 발현)

  • Lee, Eun-Mo;Choi, Shin-Geon
    • Journal of Industrial Technology
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    • v.29 no.B
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    • pp.115-119
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    • 2009
  • Green fluorescent protein (GFPuv) was displayed on the surface of ethanol-producing bacteria Zymomonas mobilis using N-terminal domain of ice nucleation protein (INP) as an anchoring motif. To evaluate the ice nucleation protein as plausible anchor motif in Z. mobilis, GFPuv gene was subcloned into Zymomonas expression vector yielding pBBR1MCS-3/pPDC/INPN/GFPuv plasmid., INP-GFPuv fusion protein was expressed in Z. mobilis and its fluorescence was verified by confocal microscopy. The successful display of GFPuv on Zymomonas mobilis suggest that INP anchor motif could be used for future fusion partner in Z. mobilis strain improvement.

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