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http://dx.doi.org/10.3807/KJOP.2011.22.1.046

The Development of Confocal Microscopy Using the Amplified Double-compound Flexure Guide  

Lee, Sang-Won (Laser and Bio Engineering Laboratory, School of Mechanical Engineering, Kyungpook National University)
Kim, Wi-Han (Laser and Bio Engineering Laboratory, School of Mechanical Engineering, Kyungpook National University)
Jung, Young-Dae (Division of Nano & Convergence Technology, National Research Foundation of Korea)
Park, Min-Kyu (School of Mechanical & Automotive Engineering Technology, Yeungnam College of Science & Technology)
Kim, Jee-Hyun (School of Electronics Engineering, Kyungpook National University)
Lee, Sang-In (Wonilfatem corporation)
Lee, Ho (Laser and Bio Engineering Laboratory, School of Mechanical Engineering, Kyungpook National University)
Publication Information
Korean Journal of Optics and Photonics / v.22, no.1, 2011 , pp. 46-52 More about this Journal
Abstract
A confocal microscope was developed utilizing a scanning sample stage based on a home-built double-compound flexure guide. A scanning sample stage with nano-scale resolution consisted of a double leaf spring based flexure, a displacement amplifying lever, a Piezo-electric Transducer(PZT) actuator and capacitance sensors. The performance of the two-axis stage was analyzed using a commercial finite element method program prior to the implementation. A single line laser was employed as the light source along with the Photo Multiplier Tube(PMT) that served as the detector. The performance of the developed confocal microscope was evaluated with a mouse ear skin imaging test. The designed scanning stage enabled us to build the confocal microscope without the two optical scanning mirror modules that are essential in the conventional laser scanning confocal microscope. The elimination of the scanning mirror modules makes the optical design of the confocal microscope simpler and more compact than the conventional system.
Keywords
Confocal microscopy; Flexure-guided stage;
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