• Title/Summary/Keyword: Confocal Scanning Microscope

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Surface profiling by the phase shifting method in fiber-optical confocal scanning interference microscopes (광섬유 공초점 간섭 현미경과 위상 변위법을 결합한 표면 검색)

  • 김대찬;이승걸
    • Korean Journal of Optics and Photonics
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    • v.10 no.3
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    • pp.201-207
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    • 1999
  • The fiber-optical confocal scanning interference microscope with a simple configuration was constructed with a 4-port fiber-optic coupler, and the new method based on the phase shifting method was proposed for surface profiling by the system. In the method, the height of a specimen was determined from the phase of confocal beam. It was verified experimentally that the method was applicable to even the confocal interference microscope with a long-wavelength source and a low NA objective, and that the scanning time could be drastically reduced compared with the conventional method. Finally, it was found that our method is less sensitive to the variation of surface reflectivity than the conventional method.

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Quantification of Melanin Density at Epidermal Basal Layer by Using Confocal Scanning Laser Microscope (CSLM) (Confocal Scanning Laser Microscope (CSLM)을 이용한 피부 기저층 멜라닌 밀도의 정량화)

  • Kim, Dong Hyun;Lee, Sung Ho;Oh, Myoung Jin;Choi, Go Woon;Yang, Woo Chul;Park, Chang Seo
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.40 no.3
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    • pp.259-268
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    • 2014
  • Non-invasive technologies in skin research have enabled to use a live image of living skin without a biopsy or histologic processing of tissue. Confocal scanning laser microscope (CSLM) operated at a near-infrared wavelength of 830 nm allows visualization of inner structure of skin as a non-invasive manner. According to previous researches using CSLM, melanin cap and papillary ring were clearly observed in pigmented areas between stratum basale and papillary dermis. In this study, conversional analysis of CSLM digital images into numerical estimation using scanning probe image processor (SPIP) software was attempted for the first time. It is concluded that a quantification of CSLM images can pave way to expand the field of applications of CSLM.

The Development of Confocal Microscopy Using the Amplified Double-compound Flexure Guide (레버 증폭 구조의 플렉서를 이용한 공초점 현미경의 개발)

  • Lee, Sang-Won;Kim, Wi-Han;Jung, Young-Dae;Park, Min-Kyu;Kim, Jee-Hyun;Lee, Sang-In;Lee, Ho
    • Korean Journal of Optics and Photonics
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    • v.22 no.1
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    • pp.46-52
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    • 2011
  • A confocal microscope was developed utilizing a scanning sample stage based on a home-built double-compound flexure guide. A scanning sample stage with nano-scale resolution consisted of a double leaf spring based flexure, a displacement amplifying lever, a Piezo-electric Transducer(PZT) actuator and capacitance sensors. The performance of the two-axis stage was analyzed using a commercial finite element method program prior to the implementation. A single line laser was employed as the light source along with the Photo Multiplier Tube(PMT) that served as the detector. The performance of the developed confocal microscope was evaluated with a mouse ear skin imaging test. The designed scanning stage enabled us to build the confocal microscope without the two optical scanning mirror modules that are essential in the conventional laser scanning confocal microscope. The elimination of the scanning mirror modules makes the optical design of the confocal microscope simpler and more compact than the conventional system.

Measurement of the Internal Structure of an Optical Waveguide Embedded in a Flexible Optical Circuit Board by Enhancing the Signal Contrast of a Confocal Microscope

  • Lee, Won-Jun;Kim, Dae-Chan;O, Beom-Hoan;Park, Se-Geun;Lee, El-Hang;Lee, Seung-Gol
    • Journal of the Optical Society of Korea
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    • v.15 no.1
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    • pp.9-14
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    • 2011
  • In this study, the internal structure of an optical waveguide embedded in a flexible optical circuit board is observed with a confocal microscope. In order to increase the light reflection from an internal material interface with a very small index difference, and thus enhance the signal contrast, a theta microscopy scheme has been integrated into a conventional confocal microscope, and a high NA oil-immersion lens has been used. The interface reflectivity is increased from roughly 0.0015% to 0.025% by the proposed method, and the internal structure can thus be successfully measured.

New Measurement of Whitening Effects by Using Confocal Scanning Laser Microscope (CSLM) (Confocal Scanning Laser Microscope (CSLM)을 이용한 신규 미백 효과 측정 연구)

  • Kim, Myong Ki;Cho, Seok-Cheol;Nam, Gaewon
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.3
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    • pp.279-285
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    • 2015
  • Hyperpigmentation on face is a highly anxiety-producing symptom, especially for women from the aspect of beauty. Pigmentation of the skin is related to the amount of melanin that provides protection against UV radiation. In vivo reflectance confocal microscopy is a non-invasive imaging tool allowing visualization of the skin without tissue alteration, by placing a microscopy directly on the living skin. The aim of this study was to develop the new evaluation method of whitening effects using in vivo reflectance confocal microscopy and to validate other instruments for measuring skin colors, and UV-induced hyperpigmentation was elicited on the inside skin of the forearm. It suggested that the new method for whitening effects using the confocal microscopy was useful to evaluate the de-pigmentation products and to easy for understanding to customers.

Fiber Orientation in Injection-Molded Short Fiber Composites with a Confocal Laser Scanning Microscope and Numerical Simulation (공초점 레이저 주사 현미경을 이용한 단섬유 복합재료 사출 성형물 내의 섬유 배열 측정 및 수치모사)

  • Lee, Kwang-Seok;Le, Seok-Won;Youn, Jae-Ryoun
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2001.05a
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    • pp.201-204
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    • 2001
  • A Confocal Laser Scanning Microscope (CLSM) is applied to determine three-dimensional fiber orientation states in injection-molded short fiber composites. Since the CLSM optically sections the composites, more than two planes either on or below the surface of composites can be obtained. Therefore, three dimensional fiber orientation states are determined without destruction. To predict the orientation states, velocity and temperature fields are calculated by using a hybrid FEM/FDM method. The change of orientation state during packing stage is also considered by employing a compressible Hele-Shaw model. The predicted orientation states show good agreement with measured ones. However, some differences are found at the end of cavity. They may result from other effects, which are not considered in the numerical analysis.

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Measurement of metal materials structure by using the manufactured Scanning Confocal Microscopy (초소형 공초점 현미경의 제작과 금속의 구조 측정)

  • Seo, Myeong-Hee;Kim, Jong-Bae;Kwon, Nam-Ic
    • Journal of the Korean Society for Precision Engineering
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    • v.25 no.11
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    • pp.52-57
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    • 2008
  • We demonstrate the operation of an apparatus that we call the laser scanning confocal microscopy. It is valuable tool of the investigations for imaging process. We measured the thin metal structure through the SCM manufacture. Confocal microscopy offers several advantages including shallow depth of field, elimination of out-of-focus glare, and the ability to collect serial optical sections from thick specimens than conventional optical microscope. This research is manufactured of scanning confocal microscopy and after measured of metal materials structure.

THE EFFECT OF MULTIPLE APPLICATION ON MICROTENSILE BOND STRENGTH OF ALL-IN-ONE DENTIN ADHESIVE SYSTEMS (All-in-one adhesive의 다층적용이 미세인장결합강도에 미치는 영향)

  • Son, Sung-Ae;Hur, Bock
    • Restorative Dentistry and Endodontics
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    • v.29 no.5
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    • pp.423-429
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    • 2004
  • The purpose of this study was to evaluate the effect of multiple application of all-in-one dentin adhesive system on microtensile bond strength using confocal laser scanning microscope and microtensile bond strength test. Flat occlusal dentin surfaces were prepared using low-speed diamond saw. In group I, Scotchbond Multipurpose (SM) was applied by manufacturer's recommendation. In group II, after Adper Prompt L-Pop was applied for 15s and light cured for 10s. the second coat was re-applied and light-cured. In group III, after light-curing the second layer. the third coat was re-applied and light-cured. Specimens bonded with a resin-composite were sectioned into resin-dentin stick for measuring the adhesive layer thickness by confocal laser scanning microscope and evaluating micro-tensile bond strength. The adhesive layers of three-step dentin adhesive system. 3 coats of Adper Prompt L-Pop had significantly thicker than SM. 2 coats of Adper Prompt L-Pop (p < 0.05). However. there was no significant differences in bond strengths between SM and 3 coats of Adper Prompt L-Pop (p > 0.05). And SM. 3 coats of Adper Prompt L-Pop had significantly higher than 2 coats of Adper Prompt L-Pop in bond strengths (p < 0.05).

Characterization of Fracture Roughness in Coarse.medium.fine Grained Granite (암반 불연속면의 거칠기 특성 - 조.중.세립질 화강암을 중심으로 -)

  • 김종태;정교철;김만일;송재용;박창근
    • The Journal of Engineering Geology
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    • v.14 no.2
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    • pp.147-168
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    • 2004
  • Purpose of this study is to quantitatively characterize the fracture roughness which was measured with a confocal laser scanning microscope. The roughness discrete data measured by confocal laser microscope were analyzed by spectral analysis and fast Fourier transform (FFT).The roughness data by used noise reduction filter were applied for fractal analysis to describe roughness features quantitatively. Artificial fractures created by Brazilian test on granites were used to measure fracture roughness under the confocal laser scanning microscope. Measurements were performed along three scan lines on each fracture surface. 36 scan lines were determined on 12 specimens in total. Features of roughness showed that coarse and medium grained granites tend to more rough features than those of fine grained granites. Continuous analog data of roughness is possible to described as discrete data of measure roughness with a fixed interval under the confocal laser microscope. Results of FFT with the measured data showed the highest values on the second harmonics. Distribution of average amplitude of second harmonics was observed 0.9853 in coarse grained granite, 1.0792 in medium grained granite and 0.6794 in fine grained granite. This indicates that the larger roughness has the higher energy of harmonics as the result of fractal analysis in low frequency zone.