Browse > Article
http://dx.doi.org/10.15230/SCSK.2014.40.3.259

Quantification of Melanin Density at Epidermal Basal Layer by Using Confocal Scanning Laser Microscope (CSLM)  

Kim, Dong Hyun (Department of Chemical and Biochemical Engineering, Dongguk University)
Lee, Sung Ho (Department of Physics, Dongguk University)
Oh, Myoung Jin (Department of Chemical and Biochemical Engineering, Dongguk University)
Choi, Go Woon (Department of Chemical and Biochemical Engineering, Dongguk University)
Yang, Woo Chul (Department of Physics, Dongguk University)
Park, Chang Seo (Department of Chemical and Biochemical Engineering, Dongguk University)
Publication Information
Journal of the Society of Cosmetic Scientists of Korea / v.40, no.3, 2014 , pp. 259-268 More about this Journal
Abstract
Non-invasive technologies in skin research have enabled to use a live image of living skin without a biopsy or histologic processing of tissue. Confocal scanning laser microscope (CSLM) operated at a near-infrared wavelength of 830 nm allows visualization of inner structure of skin as a non-invasive manner. According to previous researches using CSLM, melanin cap and papillary ring were clearly observed in pigmented areas between stratum basale and papillary dermis. In this study, conversional analysis of CSLM digital images into numerical estimation using scanning probe image processor (SPIP) software was attempted for the first time. It is concluded that a quantification of CSLM images can pave way to expand the field of applications of CSLM.
Keywords
CSLM; melanin content; SPIP;
Citations & Related Records
연도 인용수 순위
  • Reference
1 S. Gonzalez and Z. Tannous, Real-time, in vivo confocal reflectance microscopy of basal cell carcinoma, J. Am. Acad. Dermatol., 47, 869 (2002).   DOI   ScienceOn
2 M. Daiane, S. Carlos, and M. Patricia, Biophysical, morphological and clinical characterizatioin of photodamaged skin for the development of effective cosmetic formulations, Proceedings 22nd IFSCC Congress, Brazil, 679 (2013).
3 A. I. Guzman, D. Boudier, J. Breugnot, D. Rondeau, M. Guillou, and B. Closs, An innovative, rapid, qualitative and quantitative approach to the epidermal barrier function with in vivo confocal laser scanning microscopy, IFSCC MAGAZINE, 16(3), 157 (2013).
4 M. Ardigo, N. Cameli, E. Berardesca, and S. Gonzalez, Characterization and evaluation of pigment distribution and response to therapy in melasma using in vivo reflectance confocal microscopy: a preliminary study, J. Eur. Acad. Dermatol. Venereol., 24, 1296 (2010).   DOI   ScienceOn
5 S. G. Lagarrigue, J. George, E. Questel, C. Lauze, N. Meyer, J. Lagarde, M. Simon, A. Schmitt, G. Serre, and C. Paul, In vivo quantification of epidermis pigmentation and dermis papilla density with reflectance confocal microscopy: variations with age and skin phototype, Exp. Dermatol., 21, 281 (2012).   DOI
6 A. Nwaneshiudu, C. Kuschal, F. H. Sakamoto, R. Anderson, K. Schwarzenberger, and R. C. Young, Introduction to confocal microscopy, J. Invest. Dermatol., 132, 1 (2012).   DOI   ScienceOn
7 L. Liu, F. Besenbacher, and M. Dong, Nucleic acid nanotechnology, eds. J. Kjems, E. Ferapontova, and K. V. Gothelf, 29, 3, Springer, Berlin Heidelberg (2014).
8 A. Godi, A. Kuhle, and L. D. Chiffre, A new procedure for characterizing textured surfaces with a deterministic pattern of valley features, Meas. Sci. Technol., 24, 085009 (2013).   DOI   ScienceOn
9 C. Yuan, Z. Peng, and X. Yan, Surface characterization using wavelet theory and confocal laser scanning microscopy, J. Tribol., 127(2), 394, (2005).   DOI   ScienceOn
10 E. Wurm, C. Longo, C. Curchin, H Soyer, T. Prow, and G. Pellacani, In vivo assessement of chronological ageing and photoageing in forearm skin using reflectance confocal microscopy, Br. J. Dermatol., 167(2), 270 (2012).   DOI   ScienceOn
11 S. Deviant, The practically cheating statictics handbook, The Sequel (2nd Edition), Andale LLC (2010).
12 E. Wurm, C. Longo, C. Curchin, H. Soyer, T. Prow, and G. Pellacani, In vivo assessement of chronological ageing and photoageing in forearm skin using reflectance confocal microscopy, Br. J. dermatol., 267, 270 (2012).