• Nutrition Research and Practice
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• 제15권1호
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• pp.12-25
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• 2021

BACKGROUND/OBJECTIVES: The study was conducted to investigate the efficacy of the combination treatment of phytochemical resveratrol and the anticancer drug docetaxel (DTX) on prostate carcinoma LNCaP cells, including factors related to detailed cell death mechanisms. MATERIALS/METHODS: Using 2-dimensional monolayer and 3-dimensional spheroid culture systems, we examined the effects of resveratrol and DTX on cell viability, reactive oxygen species (ROS) levels, mitochondrial membrane potential, apoptosis, and necroptosis by MTT, flow cytometry, and Western blotting. RESULTS: At concentrations not toxic to normal human prostate epithelial cells, resveratrol effectively decreased the viability of LNCaP cells depending on concentration and time. The combination treatment of resveratrol and DTX exhibited synergistic inhibitory effects on cell growth, demonstrated by an increase in the sub-G0/G1 peak, Annexin V-phycoerythrin positive cell fraction, ROS, mitochondrial dysfunction, and DNA damage response as well as concurrent activation of apoptosis and necroptosis. Apoptosis and necroptosis were rescued by pretreatment with ROS scavenger N-acetylcysteine. CONCLUSIONS: We report resveratrol as an adjuvant drug candidate for improving the outcome of treatment in DTX therapy. Although the underlying mechanisms of necroptosis should be investigated comprehensively, targeting apoptosis and necroptosis simultaneously in the treatment of cancer can be a useful strategy for the development of promising drug candidates.

• Wind and Structures
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• 제37권3호
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• pp.191-209
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• 2023

This work investigates the subcritical free-shear prism wake at Re=22,000 by the Koopman analysis using the Dynamic Mode Decomposition (DMD) algorithm. The Koopman model linearized nonlinearities in the stochastic, homogeneous anisotropic turbulent wake, generating temporally orthogonal eigen tuples that carry meaningful, coherent structures. Phenomenological analysis of dominant modes revealed their physical interpretations: Mode 1 renders the mean-field dynamics, Modes 2 describes the roll-up of the Strouhal vortex, Mode 3 describes the Bloor-Gerrard vortex resulting from the Kelvin-Helmholtz instability inside shear layers, its superposition onto the Strouhal vortex, and the concurrent flow entrainment, Modes 6 and 10 describe the low-frequency shedding of turbulent separation bubbles (TSBs) and turbulence production, respectively, which contribute to the beating phenomenon in the lift time history and the flapping motion of shear layers, Modes 4, 5, 7, 8, and 9 are the relatively trivial harmonic excitations. This work demonstrates the Koopman analysis' ability to provide insights into free-shear flows. Its success in subcritical turbulence also serves as an excellent reference for applications in other nonlinear, stochastic systems.

• Tuberculosis and Respiratory Diseases
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• 제51권3호
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• pp.270-274
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• 2001

성대 운동이상에 의한 상기도 폐색은 드문질환으로 저자들은 호흡곤란을 주소로 내원한 61세 여자환자에서 기관지내시경검사와 폐기능검사로 폐쇄성폐질환에 동반된 성대 운동이상에 의한 기능성상기도 폐색증을 확진하고 이완요법(relaxation)과 산소공급으로 증상이 호전되어 퇴원후 외래에서 추적관찰중인 1예를 경험하여 이를 문헌고찰과 함께 보고하는 바이다.

• 한국경영과학회:학술대회논문집
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• 한국경영과학회 2001년도 추계학술대회 논문집
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• pp.25-28
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• 2001

Today enterprises are bringing forward the strong needs for the Global Work Space that is able to realize the collaboration of the virtual enterprise in order to achieve the rapid entrance to market, the quality improvement as well as the cost reduction of their new products. Especially, they are building the efficient Product Management Infrastructure in parallel with the real-time knowledge management for the information generated in the course of a product lifecycle and the Process Innovation making the Concurrent Engineering possible. Building a system in the web environment cannot be the entire effort to realize the Global Work Space within an enterprise that is an essential factor for the reduction of a product development period which in turn contributes to Time to Market. Various work models and processes are found in enterprises and many different application programs are developed and utilized to support these. This study proposes a scheme for the optimized Collaborative Workflow System Architecture that is able to take in and apply various application programs accompanied by the product development work process. Through this, we are to examine various limits and problems existing in the real-time collaborative system between enterprises and to reform these.

• 한국정보과학회논문지:소프트웨어및응용
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• 제26권9호
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• pp.1105-1113
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• 1999

객체 지향 소프트웨어의 확장과 더불어 소프트웨어의 재사용성의 중요도가 부각되면서 소프트웨어를 콤포넌트 단위로 구현하는 추세이다. 따라서 콤포넌트 기반의 소프트웨어 개발에서의 통합 테스트가 중요한 이슈로 떠오르고 있다. 그러나 콤포넌트들의 통합 테스트에 대한 연구는 미흡한 상태이다. 본 논문에서는 UML을 기반으로 체계적인 통합 테스트 모형을 제안하고. 나아가 본 논문에서는 "멀티미디어 정보처리 시스템"의 사례를 본 논문의 콤포넌트 통합 테스트 모형에 적용한 결과를 분석하여 기술한다. 콤포넌트 통합 테스트 모형은 UML(Unified Modeling Language)의 순서도(sequence diagram)와 협력도(collaboration diagram)를 이용하여 전체 시스템에서 UML의 사건흐름을 구성하는 콤포넌트들 사이의 인터페이스 영역에 존재하는 오류들을 추출한다. 그리고 UML을 기반으로 통합 테스트를 수행함으로써, 테스트 준비 작업을 줄이고, 기존의 UML 지원 도구들과 연계하여 테스트 자동화 도구의 구현을 앞당길 수 있다. 또한 시스템의 순차적 흐름 뿐 아니라, 동시에 수행되는 흐름에 대한 정보까지 모두 수용하여 테스트함으로써, 콤포넌트 기반의 분산 환경의 특성에 적합하다. Abstract As the object-oriented approach to software development becomes more mature, software development from pre-existing, independently developed components becomes an important aim of software engineering. Therefore, integration testing becomes an important aspect of component-based software development. However, there has been little work done in the area of the component-based integration testing. In this paper, we propose the "component integration test model" which is based on UML. Furthermore, we describe a case study on "Multimedia Information Processing System" conducted to analyse the result from which our model is applied. Our model extracts the faults, which exist in interfaces of components, using sequence diagram and collaboration diagram of UML(Unified Modeling Language). As our model is based on UML, the preparation effort for testing is reduced and its test-tools can be implemented more easily through linking existing UML tool. And our model accepts the information of concurrent flow represented by collaboration diagram as well as sequential flow, so it is more suitable to component-based distributed environment.based distributed environment.

• 한국환경과학회지
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• 제24권4호
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• pp.371-381
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• 2015

In the mountain streams in Jeju Island, strong turbulence and roughness usually made it nearly impossible to utilize most of intrusive instrumentation for streamflow discharge measurements. Instead, a non-intrusive fixed electro-magnetic wave surface velocimetry (fixed EWSV: Kalesto) became alternatively popular in many representative streams to measure stream discharge seamlessly. Currently, Kalesto has shown noteworthy performance with little loss in flood discharge measurements and also has successfully provided discharge for every minute. However, Kalesto has been operated to regard its measured one-point velocity as the representative mean velocity for the given cross-section. Therefore, it could be highly possible to potentially encompass discharge measurements errors. In this study, we analyzed the difference between such Kalesto discharge measurements and other alternative concurrent discharge measurements such as Acoustic Doppler Current Profiler (ADCP) and mobile EWSV which were able to measure velocity in multi-points in the cross-section. Consequently, Kalesto discharge deviated from ADCP discharge in amount of 48% for relatively low flow, and more than 20% difference for high flow compared with mobile EWSV discharge measurements. These results indicated that the one-point velocity measured by Kalesto should be used as a cross-sectional mean velocity, rather it should be accounted for as an index-velocity in conjunction with directly measured cross-sectional mean velocity by using more reliable instrumentations. After inducing Kalesto Discharge Correction Coefficient (KDCC) that actually means relationship between index velocity and cross-sectional mean velocity, the corrected discharge from Kalesto was significantly improved. Therefore, we found that index velocity method should be applied to obtain better accuracy of discharge measurement in case of Kalesto operation.

• Smart Structures and Systems
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• 제30권1호
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• pp.49-62
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• 2022

Experimental and discrete element methods were used to investigate the effects of triple joints lengths and triple joint angle on the failure behavior of rock mass under uniaxial compressive test. Concrete samples with dimension of 20 cm × 20 cm × 5 cm were prepared. Within the specimen, three imbedded joint were provided. The joint lengths were 2 cm, 4cm and 6 cm. In constant joint lengths, the angle between middle joint and other joints were 30°, 60°, 90°, 120° and 150°. Totally 15 different models were tested under compression test. The axial load rate on the model was 0.05 mm/min. Concurrent with experimental tests, the models containing triple joints, length and joint angle are similar to the experiments, were numerical by Particle flow code in two dimensions (PFC2D). Loading rate in numerical modelling was 0.05 mm/min. Tensile strength of material was 1 MPa. The results show that the failure behaviors of rock samples containing triple joints were governed by both of the angle and the length of the triple joints. The uniaxial compressive strengths (UCS) of the specimens were related to the fracture pattern and failure mechanism of the discontinuities. Furthermore, it was shown that the compressive behavior of discontinuities is related to the number of the induced tensile cracks which are increased by decreasing the joint length. Along with the damage failure of the samples, the acoustic emission (AE) activities are excited. There were only a few AE hits in the initial stage of loading, then AE hits rapidly grow before the applied stress reached its peak. In addition, every stress drop was accompanied by a large number of AE hits. Finally, the failure pattern and failure strength are similar in both methods i.e., the experimental testing and the numerical simulation methods.

• 재무관리연구
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• 제27권2호
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• pp.169-204
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• 2010

본 논문에서는 한국금융시장의 자료를 이용하여 펀드플로우와 시장위험간의 관계를 검증하고, 펀드플로우의 변화가 시장의 위험수준의 변화를 설명할 수 있는지를 분석하였다. 이는 펀드플로우와 시장위험간의 관계에 대한 학문적 시사점을 제공하고 일각에서 제기하고 있는 펀드런에 의한 시스템리스크 유발가능성을 탐색한다는 점에서 의미를 갖는다. 주식형 펀드플로우와 주식시장 위험에 대한 분석결과는 펀드자금의 유입이 시장위험과 (+)의 관계를 가짐을 보여준다. 채권형 펀드의 경우 펀드플로우는 채무불이행위험프리미엄과 음(-)의 관계를, 기간프리미엄과는 양(+)의 관계를 갖는다. MMF의 결과는 MMF로의 자금유입이 시장의 유동성위험을 줄여줌을 보여준다. 예측오차의 분산분해를 통한 전이지수의 구성을 통해 펀드플로우의 변화가 시장위험의 변화를 얼마나 설명할 수 있는지를 분석한 결과는 설명력이 제한적이며 변동이 매우 심한 결과를 보여준다. 주식시장의 경우 한국자본시장에서 서브프라임 사태의 영향이 본격화된 시기인 2007년 말 이후 펀드플로우에 가해진 변화가 시장의 위험변동을 설명하는 비율이 상대적으로 크게 증가해 이러한 추세가 상당기간 지속되는 모습을 보인다. 반면, 채권시장의 경우 2008년 말 이후 펀드플로우에 가해진 충격이 채권시장의 위험에 전이되는 현상이 지속적으로 나타나며, 단기 금융시장의 경우에는 이러한 현상이 체계적으로 발생하지 않는다. 주식시장과 채권시장에서 보인 특정시기를 중심으로 하는 전염효과의 지속현상은 펀드플로우에 가해진 예상치 못한 충격이 시장위험을 증가시킬 수 있음을 의미한다. 그러나 회귀분석과 VAR 모형의 추정결과, 그리고 분산분해의 설명력 등을 고려하여 판단할 때 본 연구의 결과는 펀드플로우의 변화가 시장위험의 변동을 설명하는 설명력이 제한적이어서 일부에서 우려하는 펀드런에 따른 금융시장의 시스템리스크의 증가와 전반적인 위기의 확산으로 나타날 가능성은 높지 않음을 말해준다.

• Tuberculosis and Respiratory Diseases
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• 제75권1호
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• pp.9-17
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• 2013

Background: In cancer cells, autophagy is generally induced as a pro-survival mechanism in response to treatment-associated genotoxic and metabolic stress. Thus, concurrent autophagy inhibition can be expected to have a synergistic effect with chemotherapy on cancer cell death. Monensin, a polyether antibiotic, is known as an autophagy inhibitor, which interferes with the fusion of autophagosome and lysosome. There have been a few reports of its effect in combination with anticancer drugs. We performed this study to investigate whether erlotinib, an epidermal growth factor receptor inhibitor, or rapamycin, an mammalian target of rapamycin (mTOR) inhibitor, is effective in combination therapy with monensin in non-small cell lung cancer cells. Methods: NCI-H1299 cells were treated with rapamycin or erlotinib, with or without monensin pretreatment, and then subjected to growth inhibition assay, apoptosis analysis by flow cytometry, and cell cycle analysis on the basis of the DNA contents histogram. Finally, a Western blot analysis was done to examine the changes of proteins related to apoptosis and cell cycle control. Results: Monensin synergistically increases growth inhibition and apoptosis induced by rapamycin or erlotinib. The number of cells in the sub-$G_1$ phase increases noticeably after the combination treatment. Increase of proapoptotic proteins, including bax, cleaved caspase 3, and cleaved poly(ADP-ribose) polymerase, and decrease of anti-apoptotic proteins, bcl-2 and bcl-xL, are augmented by the combination treatment with monensin. The promoters of cell cycle progression, notch3 and skp2, decrease and p21, a cyclin-dependent kinase inhibitor, accumulates within the cell during this process. Conclusion: Our findings suggest that concurrent autophagy inhibition could have a role in lung cancer treatment.

• 한국통신학회논문지
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• 제30권7B호
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• pp.489-498
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• 2005

서비스 거부 공격은 네트워크 자원을 독점하여 서버 시스템 및 네트워크상에 인위적인 혼잡을 발생시키는 공격으로써, 일반 사용자가 정상적인 서비스를 제공받지 못하도록 하는 것을 목적으로 한다. 본 논문에서는 인위적인 네트워크 혼잡 상황에서도 정상 트래픽의 서비스 품질을 보장할 수 있는 소수자 우선 게이트웨이를 제안한다. 소수자 우선 게이트웨이는 어떤 집합 플로우가 혼잡유발 트래픽인지를 빠르게 결정할 수 있는 방법을 제공한다. 소수자 우선 게이트웨이는 정상 트래픽으로 판정된 집합 플로우에게는 높은 우선순위의 서비스를 제공하여 그 품질을 보호하고, 혼잡유발 트래픽으로 판정된 집합 플로우에게는 낮은 우선순위의 서비스를 제공하여 네트워크 혼잡이 혼잡유발 트래픽에게만 영향을 미치도록 한다. 제안하는 소수자 우선 게이트웨이는 서비스 거부 공격 뿐만 아니라 다중 노드에서 동시에 혼잡을 일으키는 분산 서비스 거부 공격에서도 정상 트래픽의 서비스 품질을 보장하는 효과를 제공함을 시뮬레이션을 통하여 확인하였다.