• Title/Summary/Keyword: Concentration gradient

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Quantitative Analysis of Single Bacterial Chemotaxis Using a Hydrodynamic Focusing Channel (유체역학적 집속 효과를 이용한 단일 박테리아 주화성의 정량적 분석)

  • Jeon, Ho-Jeong;Lee, Yong-Ku;Jin, Song-Wan;Koo, Sang-Mo;Lee, Chang-Soo;Yoo, Jung-Yul
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.31 no.3 s.258
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    • pp.209-216
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    • 2007
  • Bacterial chemotaxis is essential to the study of structure and function of bacteria. Although many studies have accumulated the knowledge about chemotaxis in the past, the motion of a single bacterium has not been studied much yet. In this study, we have developed a device microfabricated by soft lithography and consisting of microfluidic channels. The microfluidic assay generates a concentration gradient of chemoattractant linearly in the main channel by only diffusion of the chemicals. Bacteria are injected into the main channel in a single row by hydrodynamic focusing technique. We measured the velocity of bacteria in response to a given concentration gradient of chemoattractant using the microfludic assay, optical systems with CCD camera and simple PTV (Particle Tracking Velocimetry) algorithm. The advantage of this assay and experiment is to measure the velocity of a single bacterium and to quantify the degree of chemotaxis by statistically analyzing the velocity at the same time. Specifically, we measured and analyzed the motility of Escherichia coli strain RP437 in response to various concentration gradients of L-aspartate statistically and quantitatively by using this microfluidic assay. We obtained the probability density of the velocity while RP437 cells are swimming and tumbling in the presence of the linear concentration gradient of L-aspartate, and quantified the degree of chemotaxis by analyzing the probability density.

Forecasting daily PM10 concentrations in Seoul using various data mining techniques

  • Choi, Ji-Eun;Lee, Hyesun;Song, Jongwoo
    • Communications for Statistical Applications and Methods
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    • v.25 no.2
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    • pp.199-215
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    • 2018
  • Interest in $PM_{10}$ concentrations have increased greatly in Korea due to recent increases in air pollution levels. Therefore, we consider a forecasting model for next day $PM_{10}$ concentration based on the principal elements of air pollution, weather information and Beijing $PM_{2.5}$. If we can forecast the next day $PM_{10}$ concentration level accurately, we believe that this forecasting can be useful for policy makers and public. This paper is intended to help forecast a daily mean $PM_{10}$, a daily max $PM_{10}$ and four stages of $PM_{10}$ provided by the Ministry of Environment using various data mining techniques. We use seven models to forecast the daily $PM_{10}$, which include five regression models (linear regression, Randomforest, gradient boosting, support vector machine, neural network), and two time series models (ARIMA, ARFIMA). As a result, the linear regression model performs the best in the $PM_{10}$ concentration forecast and the linear regression and Randomforest model performs the best in the $PM_{10}$ class forecast. The results also indicate that the $PM_{10}$ in Seoul is influenced by Beijing $PM_{2.5}$ and air pollution from power stations in the west coast.

Double-Diffusive Convection Due to Heating from Below in a Rotating Cylindrical Cavity (회전하는 원통형밀폐용기내의 아랫면가열에 의한 이중확산대류에 관한 실험적 연구)

  • 강신형;이태홍;이진호
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.19 no.7
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    • pp.1731-1740
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    • 1995
  • Experimental investigations have been made to study the double-diffusive nature of convection of an initially stratified salt-water solution due to heating from below in a rotating cylindrical cavity. The objective is to examine the flow phenomena and the heat transfer characteristics according to the changes in temperature gradient, concentration gradient and rotating velocity of cavity. Thermal and solutal boundary conditions at side wall are adiabatic and impermeable, respectively. The top and bottom plate are maintained each at constant temperature and concentration. The cavity is put into a state of solid body rotation. Like the stationary case, the types of initially-formed flow pattern are classified into three regimes depending on the effective Rayleigh number and Taylor number; stagnant flow regime, single mixed-layer flow regime and successively formed multi-mixed layer flow regime. At the same effective Rayleigh number, the number of initially-formed mixed layer and its growth rate decrease as the effect of rotation increases. The temperature and concentration profiles are both uniform in each layer due to convective mixing in the layered-flow regime, but look both liner in stagnant flow regime and single mixed-layer flow regime. At the interface between adjacent layers, the temperature changes smoothly but the concentration changes rapidly.

The Finite Element Analysis on the Characteristics of the Hydrogen Diffusion for the Cr-Mo Steels (Cr-Mo강의 수소확산 특성에 관한 유한요소해석)

  • Lee, Hwi-Won;Ha, Min-Su
    • Journal of the Korean Society of Mechanical Technology
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    • v.13 no.2
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    • pp.115-121
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    • 2011
  • The size of hydrogen molecule is not so small as to invade into the lattice of material, and therefore, hydrogen invades into the material as atom. Hydrogen movement is done by diffusion or dislocation movement in the near crack tip or plastic deformation. Hydrogen appeared to have many effects on the mechanical properties of the Cr-Mo steel alloys. The materials for this study are 1.25Cr-0.5Mo and 2.25Cr-1Mo steels used at high temperature and pressure. The hydrogen amount obtained by theoretical calculation was almost same with the result solved by finite element analysis. The distribution of hydrogen concentration and average concentration was calculated for a flat specimen. Also, finite element analysis was employed to simulate the redistribution of hydrogen due to stress gradient. The calculation of hydrogen concentration diffused into the material by finite element method will provide the basis for the prediction of delayed fracture of notched specimen. The distribution of hydrogen concentration invaded into the smooth and notched specimen was obtained by finite element analysis. The hydrogen amount is much in smooth specimen and tends to concentrate in the vicinity of surface. Hydrogen embrittlement susceptibility of notched specimen after hydrogen charging is more remarkable than that of smooth specimen.

Mercury Fluxes from the Nan-Ji-Do Area of Seoul -Application of Micrometerorological Methods (미기상학적 기법을 응용한 난지도지역이 수은교환율 측정연구)

  • 김민영;김기현;이강웅;정일현
    • Journal of Korean Society for Atmospheric Environment
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    • v.16 no.6
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    • pp.585-594
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    • 2000
  • Through an application of Micrometerorological methods, we conducted measurements of Hg fluxes from Nan-Ji-Do which is well known as one of the major local areal sources in Seoul metropolitan area during Match/April of 2000. In the course of our study, we determined the concentration gradients of total gaseous Hg(between 20 and 2000 cm heights) and combined these data with Micrometerorological components to derive is fluxes. It turned out that emission from and dry deposition to soil surfaces occurred at the ratio of 72:27 from a total of 271 hourly measurements. The validity of measured concentration gradients( or resulting fluxes) was evaluated in terms of percent gradient. Accordingly, about more than 95% of gradient data derived were statistically significant. The mean fluxes of Hg across soil-air interface, when computed using the concentrations gradients and relevant parameters, were found at 253(during emission) and -846ng/$m^2$/h(during dry deposition) The occurrences of abnormalously high exchange rates appear to be the combined effects of enormously high gradient values and high transfer coefficients. While the emissions of Hg occurred constantly during the whole study periods, the occurrences of dry deposition events were observed most intensively during very limited time periods(3/29 and 4/3). The results of our study cleary indicated that the studied area is a strong local areal source, while exhibiting great potential as a major sink simultaneously.

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A Study on the Ultrasonic Application for the Efficiency Elevation of the Hydrogen Fuel Production (On solution and concentration) (수소연료 생산의 효율향상을 위한 초음파응용에 관한 연구(용액과 농도 중심으로))

  • Song, Min-Geun;Lee, Sang-Bum;Son, Seung-Woo;Ju, Eun-Sun
    • Transactions of the Korean hydrogen and new energy society
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    • v.12 no.2
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    • pp.129-136
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    • 2001
  • An investigation on the A/V(ampere/volt) gradient according to the concentration and the kind of solution in a electrolyzer is carried out to obtain the basic data on the ultrasonic application for the efficiency elevation of the hydrogen fuel production. KOH is selected as an electrolyte and concentrations are 0%, 10%, 20% and 30%. The solutions are city water, city water with nitrogen. distilled water and distilled water with nitrogen. The Electrochemical analyzer(BAS Co.) is used as a measuring device to observe the A/V gradient. And the limit of volt is from -3000mV to +3000mV. The 28kHz magnetic transducer is selected to give them ultrasonic forcing. In results, it is clarified that ultrasonic influences the A/V gradient in the electrolytic solution.

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A Study on the Characteristics of Lithium Concentration Gradient between Maternal and Fetal Plasma in Rabbits (모체혈장 및 태자혈장간의 $Li^+$농도 경사의 특성에 관한 연구)

  • Shim, Jae-Sik;Sung, Ho-Kyung
    • The Korean Journal of Physiology
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    • v.21 no.1
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    • pp.23-33
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    • 1987
  • This study was carried out to investigate the characteristics of lithium concentration difference between maternal and fetal plasma and the effect of previous lithium loading on rapid transplacental transport of large amounts of lithium. Pregnant rabbits at $20{\sim}22\;days$ of gestation were divided into two groups: chronic $Li^+$ injection group and chronic plus acute $Li^+$ injection group. Small amounts of LiCl (1 mmol/kg per day) were given intraperitoneally to all rabbits of both group, for 5 days before sacrifice. The rabbits of chronic plus acute injection group, received additional intravenous injections of large amounts of LiCl (2 mmol/kg) one hour before sacrifice. Maternal arterial blood, placental sinus blood, fetal blood and amniotic fluid were drawn and analyzed for the plasma concentrations of $Li^+$, $Na^+$ and $K^+$ and for osmolartiy. Followings are the results obtained. 1) There was no difference in the $Li^+$ concentration between maternal plasma and placental sinus plasma in chronic lithium group, although the $Li^+$ concentration of placental sinus plasma was slightly lower than that of maternal arterial plasma in the chronic plus acute lithium group. 2) The $Li^+$ concentration of fetal plasma was much lower than that of placental sinus plasma in both groups, the ratio being $0.76{\pm}0.250$ ($mean{\pm}95%$ confidence interval) for the chronic $Li^+$ group and $0.78{\pm}0.366$ for the chronic plus acute $Li^+$ group. 3) The ratio of $Li^+$ concentration of fetal plasma to maternal arterial plasma was $0.71{\pm}0.196$ in the chronic group and $0.59{\pm}0.261$ in the chronic plus acute group. 4) $Li^+$ concentration of amniotic fluid was much higher than that of fetal plasma in the chronic $Li^+$ group but not significantly different in the chronic plus acute $Li^+$ group. 5) An acute loading of $Li^+$ did not produce any detectable changes in $Na^+$ and $K^+$ concentrations and osmolarity of the maternal plasma. The above results may suggest that: (a) The placental barrier maintains steady state lithium concentration gradient between placental sinus plasma and fetal plasma. (b) In rabbits chronically treated with $Li^+$ the steady state $Li^+$ gradient is established within one hour after an acute $Li^+$ loading, provided that the $Li^+$ concentration in the maternal plasma is less than 4 mmole/l.

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Mineral and Chemical Changes in Silica Brick After Service in Arc-furnace Roofs (아-크로 천장 사용후의 규석벽돌의 광물 및 화학적 변화)

  • 오기동;박홍채
    • Journal of the Korean Ceramic Society
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    • v.18 no.4
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    • pp.247-256
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    • 1981
  • Silica bricks had been in arc-furnace roofs of various sizes and steelmaking practices. The resulting materials were examined with reference to mineral and chemical changes. Silica bricks develope definite zones while in service. These zones represent a concentration gradient through the brick that results from the thermal gradient across the brick and from the furnace atmosphere. There are major brick losses by spalling as well as by melting of the hot-face surface in an iron-oxide rich liquid.

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Development of a program to predict the airflow rate and pollutant concentration in complex network-type tunnels (네트워크형 터널의 풍량 및 농도해석 프로그램 개발연구)

  • Kim, Hyo-Gyu;Choi, Pan-Gyu;Ryu, Ji-Oh;Lee, Chang-Woo
    • Journal of Korean Tunnelling and Underground Space Association
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    • v.19 no.2
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    • pp.213-229
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    • 2017
  • Recently, in urban areas there is a tendency to construct more complex network-type tunnels including entrance and exit ramps. At the same time, various one-dimensional programs based on the network theory have been proposed for tunnel ventilation analysis. This paper aims at developing a program that can analyze the ventilation flow rate and pollutants concentration in complex network-type tunnels based on the none hardy-cross method. The flow analysis in the branch was carried out on the basis of the Gradient method, while for the concentration analysis a new logic has been developed to calculate the inflow and outflow concentration automatically in a complex network-type structure. Additionally, in the tunnel segments showing low flow rate, proper grid interval sizes were proposed to reduce numerical error. To verify the applicability of the program, flow rates predicted in the straight tunnels were compared with the classical velocity-diagram method by Stokic and the TVSDM program. The results showed that the errors were within 1%. In addition, the program was applied to the recent ventilation system adopted in the complex network-type urban tunnels.

Metallurgical Refinement of Multicrystalline Silicon by Directional Solidification (일방향 응고법에 의한 다결정 실리콘의 야금학적 정련)

  • Jang, Eunsu;Park, Dongho;Yu, Tae U;Moon, Byung Moon
    • 한국신재생에너지학회:학술대회논문집
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    • 2011.05a
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    • pp.111.1-111.1
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    • 2011
  • The solar energy is dramatically increasing as the alternative energy source and the silicon(Si) solar cell are used the most. In this study, the improved process and equipment for the metallurgical refinement of multicrystalline Si were evaluated for the inexpensive solar cell. The planar plane and columnar dendrite aheadof the liquid-solid interface position caused the superior segregation of impurities from the Si. The solidification rate and thermal gradient determined the shape of dendrite in solidified Si matrix solidified by the directional solidification(DS) method. To simulate this equipment, the commercial software, PROCAST, was used to solve the solidification rate and thermal gradient. Si was vertically solidified by the DS system with Stober process and up-graded metallurgical grade or metallurgical grade Si was used as the feedstock. The inductively coupled plasma mass spectrometry (ICP) was used to measure the concentration of impurities in the refined Si ingot. According to the result of ICP and simulation, the high thermal gradient between the two phases wasable to increase the solidification rate under the identical level of refinement. Also, the separating heating zone equipped with the melting and solidification zone was effective to maintain the high thermal gradient during the solidification.

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