• 한국수산과학회지
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• 제23권6호
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• pp.425-438
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• 1990

쉘리코프 해협에서 산란하는 명태의 정확한 산란장소 및 시기를 조사하기 위하여 1981 년, 1984년, 1985년에 음향학적 조사 및 난치자어 조사를 하였다. 알라스카만의 명태는 산란하기 위하여 늦겨울 내지 초봄에 쉘리코프 해협으로 이동한다. 명태는 대륙사면으로 부터 따뜻하고 염분도가 높은 해수를 이용하여 해협 남동쪽 입구의 바닥을 타고 들어와 중앙부의 서쪽면에 있는 깊은(>200 m) 곳에 모여들고, 3월부터 산란을 한다. 최대산란은 이곳에서 3월 하순부터 4월 초순에 걸쳐 일어나고, 해협내 수괴의 성질(해수온, 염분도, 밀도 등)은 해마다 다르지만 산란시기 및 장소는 거의 변함이 없다. 이 해역은 어류의 산란장이 보육장과 가까이 있다는 지리적 이점 이외에도 해마다 이 시기에는 해수의 이동량이 최저치로 내려가고 식물플랑크톤 번식의 핵심요소인 해수의 표면 혼합층이 최소로 된다는 해양학적 현상 때문에 알라스카만 명태의 최적합 산란장이 되어왔다고 추측된다. 4월 초순 이후, 산란행위는 급격히 감소하며 산란장은 북동쪽과 남서방향으로 약간 확장됨을 보여준다.

• 한국환경과학회지
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• 제22권12호
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• pp.1539-1549
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• 2013

The purpose of this study is to compare the sampling methods for monitoring indoles (phenol, p-cresol, indole and skatole) in airs of swine facility. As the collecting methods of indoles in air, Tenax-TA adsorption tube and solid phase microextraction (SPME) were examined. For the preparation of calibration curves of indoles concentrated in Tenax-TA, the standard indoles solutions were spiked in each of Tenax-TA tubes and thermally desorbed (ATD) into a gas chromatograph combined with mass detector (GC/MS). And for the preparation of calibration curves by SPME, indoles in the standard gaseous solution prepared by evaporating the aqueous solution that contained indoles into a polyester sampling bag were extracted with SPME fiber and subsequently analyzed by the GC/MS. Two sampling methods were evaluated for extracting indoles present in swine building environments. Results indicated that the SPME method using Polydimethylsiloxane/ Divinylbenzene (PDMS/DVB) fiber was more effective than Tenax-TA method in extracting indoles. The gas chromatographic analysis showed that the linearities of calibration curves and detection limits were useful for detection of indoles in swine airs. The field tests also showed that considerably different levels of indoles were present in various parts of the swine building.

• Journal of Microbiology and Biotechnology
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• 제15권4호
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• pp.809-814
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• 2005

Gibberellins (GAs) play an important role in plant growth and development. Fifteen fungi were isolated from Physalis alkekengi var francheti plant roots, and among them, four isolates showed GA-production activity. A bioassay using waito-c rice was carried out with the culture fluid of the GA-producing fungi. The GA-producing fungi were cultured for 7 days in Czapek's liquid medium at $30^{\circ}C$, 120 rpm, under dark conditions. The culture broth was concentrated 30-fold and 10 ${\mu}l$ of that concentrate was applied to 2-leaf rice sprouts. The height of the rice seedlings treated with the culture fluid of isolate PA08 was 26 cm high, while that of the seedlings treated with the wild-type Gibberella fujikuroi was 13 cm high. As such, the plant growth-promoting activity exhibited by isolate PA08 was 2 times stronger than that exhibited by the wild-type G fujikuroi. The amounts of $GA_l,\;GA_3,\;GA_4,\;GA_7,\;GA_9,\;GA_{20}$, and $GA_{24}$ in the medium were measured using gas chromatography-mass spectrometry (GC-MS), and the quantities produced by isolate PA08 were 4.85 ng/ml, 4.79 ng/ml, 17.30 ng/ml, 6.01 ng/ml, 16.61 ng/ ml, 0.08 ng/ml, and 17.30 ng/ml, respectively. Isolate PA08 was also identified as Fusarium proliferatum KGL0401 by a genetic analysis of the nucleotide sequences of the internal transcribed spacer region of the ribosomal DNA.

• 한국미생물·생명공학회지
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• 제27권6호
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• pp.491-499
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• 1999

A great variety of the volatile metabolic by-products was formed in yeast cell during alcohol fermentation. The seibel grape (Vitis labrasca) which was grown in the Southern Korea used for wines. The objective of this research was to identify the volatile flavor compounds during alcohol fermentation and aging at 12$^{\circ}C$. saccharomyces cerevisiae and Schizosaccharomyces pombe were inoculated and fermented in seibel grape must. The volatile flavor compounds of logarithmic, stationary and death phases were extracted, concentrated and identified by gas chromatography/mass spectrometer (GC/MS). The volatile flavor compounds were determined by a Hewlett-Packard 5890 II Plus GC which was equipped with Supelcowax 10 fused silica capillary column (60m$\times$0.32mm$\times$0.25${\mu}{\textrm}{m}$ film thickness) wall coated with polyethyleneglycerol. The scan detection method allowed the comparison of the spectrum from the chromatogram of volatile flavor compounds to those in data Wileynbs base library. Among the volatile compounds collected by ether-hexane extraction method, the evolution of 20 main compounds, such as 9 esters (ethyl butyrate, isoamyl acetate, ethyl caproate, n-hexyl acetate, ethl caprylate, ethyl caprate, diethy succinate, ethyl hexadecanoate, 2-pheneethyl acetate), 4 alcohols (3-methyl-1-butanol, 1-hexanol, 1-heptanol, benzoethanol), 4 ketones and acids (2-octanone, caproic acid, caprylic acid, capric acid), 2 furan and phenol (2,6-bis(1,1-dimethyl ethyl)phenol, 2,3-dihydrobenzofuran) were observed during alcohol fermentation and aging. The production of the esters during alcohol fermentation with S. cerevisiae was higher than those of Sch. pombe. The sensory scores of the aged wine samples in aroma, taste and overall acceptability were not significantly different(p<0.05).

• 한국해양환경ㆍ에너지학회지
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• 제3권4호
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• pp.3-22
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• 2000

1998년 하계(6～9월)에 금강하구 주변 해역 11개 정점을 조사하여 공간적인 수질변통 특성을 파악하고, 통계분석 기법의 하나인 요인분석을 동하여 하계 금강하구 주변해역에서의 수질변동 지배요인을 분석하였다. 조사기간 동안 금강하구 주변해역의 화학적산소요구량의 평균농도는 1.36mg/L, 용존무기질소의 평균농도는 28.60㎍-at/L, 용존무기인의 평균농도는 0.48㎍-at/L로 나타났으며, 주성분분석 결과 전체 수질변동의 약 43%를 차지하는 제 1주성분 요인이 담수 유입과 밀접한 관련이 있는 영양염류와 염분의 변화로 나타나, 담수변통 요인이 중요한 역할을 하는 것으로 나타났다.

• Geomechanics and Engineering
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• 제22권1호
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• pp.1-9
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• 2020

This paper presents an investigation of the ground response of a gob-side gateroad suffering mining stress induced by a 21 m-thick coal seam extraction. A field observation, including entry convergence and stress changes monitoring, was first conducted in the tailgate 8209. The observation results of entry convergence showed that, during the adjacent panel 8210 retreating period, the deformation of the gob-side gateroad experienced a continuous increase stage, subsequently, an accelerating increase stage, and finally, a slow increase stage. However, strong ground response, including roof bending deflection, rib extrusion and floor heave, occurred during the current panel 8209 retreating period, and the maximum floor heave reached 1530 mm. The stress changes within coal mass of the two ribs demonstrated that the gateroad was always located in the stress concentrated area, which responsible for the strong response of the tailgate 8209. Subsequently, a hydraulic fracture technique was proposed to pre-fracture the two hard roofs above the tailgate 8209, thus decreasing the induced disturbance on the tailgate. The validity of the above roof treatment was verified via field application. The finding of this study could be a reference for understanding the stability control of the gob-side gateroad in extra thick coal seams mining.

• Journal of Applied Biological Chemistry
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• 제54권1호
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• pp.67-70
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• 2011

The roots of Glycyrrhiza uralensis Fisch. were extracted with 30% aqueous ethanol (EtOH), and the concentrated extract was partitioned with n-hexane, chloroform ($CHCl_3$), ethyl acetate (EtOAc), n-butanol (n-BuOH), and $H_2O$, successively. From the $CHCl_3$ fraction, four flavonoids were isolated through the repeated silica gel ($SiO_2$), octadecyl silica gel (ODS), and Sephadex LH-20 column chromatographies (c.c.). According to the results of spectroscopic data including nuclear magnetic resonance spectrometry (NMR), electron ionization mass spectrometry (EI/MS), and infrared spectroscopy (IR), the chemical structures of the compounds were determined as glabrol (1), abyssinone II (2), glabridin (3), and isoliquiritigenin (4). The flavonoids were evaluated for cytotoxic effect against human cancer cell lines, HCT-116, HepG2, HeLa, SK-OV-3, SK-BR-3, MCF-7, and SK-MEL-5. Especially, glabrol (1) and glabridin (2) showed $IC_{50}$ values of lower than $25{\mu}M$.

• Journal of Microbiology and Biotechnology
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• 제21권10호
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• pp.1021-1025
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• 2011

A Bacillus subtilis strain was isolated from the intestine of Sebastiscus marmoratus (scorpion fish) that was identified as Bacillus subtilis CH2 by morphological, biochemical, and genetic analyses. The chitosanase of Bacillus subtilis CH2 was best induced by fructose and not induced with chitosan, unlike other chitosanases. The strain was incubated in LB broth, and the chitosanase secreted into the medium was concentrated with ammonium sulfate precipitation and purified by gel permeation chromatography. The molecular mass of the purified chitosanase was detected as 29 kDa. The optimum pH and temperature of the purified chitosanase were 5.5 and $60^{\circ}C$, respectively. The purified chitosanase was continuously thermostable at $40^{\circ}C$. The specific acitivity of the purified chitosanase was 161 units/mg. The N-terminal amino acid sequence was analyzed for future study.

• 한국언론정보학보
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• 제38권
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• pp.245-275
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• 2007

본 연구는 방송 환경에서의 갈등 요인을 정책과 관행을 포괄하는 제도 개념을 설정하고 있는 신제도주의적 관점에 입각하여 외주 정책과 시장 행위 간의 상호작용 과정을 조망하고자 하는 데 목적이 있다. 상호작용 과정을 조망해보기 위해 일차적으로 신제도주의적 관점에서 제시하고 있는 공식적 차원의 정책과 비공식적 차원에서의 관행 간의 상호작용 과정에 입각한 메커니즘을 경로의존성 개념을 통해 조망해봄으로써 새로운 관행으로의 정착에 이르기까지 필연적으로 발생할 수밖에 없는 갈등의 과정을 행위 주체 간의 관계를 통해 분석하고자 한다. 이를 통해 정책이 어떻게 변화하고, 그에 따른 행위가 다시 어떠한 메커니즘하에서 형성되는지의 과정을 이론적 차원에서 모델링해보는 데 본 연구의 궁극적인 목적이 있다.

• 콘크리트학회논문집
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• 제21권1호
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• pp.21-27
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• 2009

본 연구는 메타카올린을 혼합한 시멘트 경화체의 황산마그네슘 침식 저항성을 평가하며, 관련 성능저하 모드를 고찰하기 위하여 수행되었다. 메타카올린의 대체율을 4단계로 조절하여 모르타르를 제조하였으며, 두 종류 황산마그네슘 용액 (0.424% 및 4.24% $MgSO_4$)에 360일 동한 침지한 후 재령별 외관조사 및 선형팽창 정도를 모니터링하였다. 아울러, XRD, DSC 및 SEM/EDS와 같은 기기분석 기법을 이용하여 황산마그네슘 용액에 침지한 메타카올린 혼합 시멘트페이스트 중에 생성된 반응생성물 조사 및 미세구조 분석을 실시하였다. 본 실험에서 수행한 실험 결과에 의하면, 메타카올린의 대체율이 증가할수록 모르타르의 황산마그네슘 저항성이 감소하는 경향을 나타내었다. 이러한 현상이 나타난 이유는 황산마그네슘 침식에 의하여 생성된 gypsum 및 thaumasite와 같은 반응생성물의 영향 때문인 것으로 판단된다.