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http://dx.doi.org/10.3839/jabc.2011.012

Cytotoxic Effect of Flavonoids from the Roots of Glycyrrhiza uralensis on Human Cancer Cell Lines  

Park, Ji-Hae (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
Wu, Qian (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
Yoo, Ki-Hyun (Department of Genetic Engineering, Kyung Hee University)
Yong, Hye-Im (Research Division of Food Industry Promotion, Korea Food Research Institute)
Cho, Sueng-Mock (Research Division of Food Industry Promotion, Korea Food Research Institute)
Chung, In-Sik (Department of Genetic Engineering, Kyung Hee University)
Baek, Nam-In (The Graduate School of Biotechnology & Plant Metabolism Research Center, Kyung Hee University)
Publication Information
Journal of Applied Biological Chemistry / v.54, no.1, 2011 , pp. 67-70 More about this Journal
Abstract
The roots of Glycyrrhiza uralensis Fisch. were extracted with 30% aqueous ethanol (EtOH), and the concentrated extract was partitioned with n-hexane, chloroform ($CHCl_3$), ethyl acetate (EtOAc), n-butanol (n-BuOH), and $H_2O$, successively. From the $CHCl_3$ fraction, four flavonoids were isolated through the repeated silica gel ($SiO_2$), octadecyl silica gel (ODS), and Sephadex LH-20 column chromatographies (c.c.). According to the results of spectroscopic data including nuclear magnetic resonance spectrometry (NMR), electron ionization mass spectrometry (EI/MS), and infrared spectroscopy (IR), the chemical structures of the compounds were determined as glabrol (1), abyssinone II (2), glabridin (3), and isoliquiritigenin (4). The flavonoids were evaluated for cytotoxic effect against human cancer cell lines, HCT-116, HepG2, HeLa, SK-OV-3, SK-BR-3, MCF-7, and SK-MEL-5. Especially, glabrol (1) and glabridin (2) showed $IC_{50}$ values of lower than $25{\mu}M$.
Keywords
abyssinone II; cytotoxicity; glabridin; glabrol; Glycyrrhiza uralensis; isoliquiritigenin;
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Times Cited By KSCI : 2  (Citation Analysis)
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