• Archives of Pharmacal Research
• /
• v.25 no.2
• /
• pp.143-150
• /
• 2002

Fluorescent probes bound to DNA typically display nanosecond decay times and reveal only nanosecond motions. We extend the time range of measurable DNA dynamics using $[Ru(pby)_2(dppz)]^{2+}$ (bpy=2.2'-bipyridine, dppz=dipyrido[3,2-a2',3'-c]phenazine) (RuBD) which displays a mean lifetime near 90 ns. To test the usefulness of RuBD as a probe for diffusive processes in calf thymus DNA, we compared the efficiencies of fluorescence resonance energy transfer (FRET) using three donors which display lifetimes near 5 ns for acridine orange (AO), 22 ns for ethidum bromide (EB) and 92 ns for RuBD, with nile blue (NB) as the acceptor. The F rster distances for AO-NB, EB-NB and RuBD-NB donor-acceptor pairs were 42.3, 52.3, and $30.6{\;}{\AA}$, respectively. All three donors showed dramatic decreases in fluorescence intensities and more rapid intensity decays with increasing NB concentrations. The intensity decays of AO and EB in the presence of varying concentrations of NB were satisfactorily described by the one-dimensional FRET model without diffusion (Blumen and Manz, 1979). In the case of the long-lifetime donor RuBD, the experimental phase and modulation somewhat deviated from the recovered values computed from this model. The recovered NB concentrations and FRET efficiencies from the model were slightly larger than the expected values, however, the recovered and expected values did not show a significant difference. Thus, it is suggested that the lifetime of RuBD is too short to measure diffusive processes in calf thymus DNA.

• Journal of Power Electronics
• /
• v.15 no.3
• /
• pp.660-669
• /
• 2015

This paper presents a control scheme with a focus on the combination of phase disposition pulse width modulation (PDPWM) and DC capacitor voltage control for a chopper-cell based modular multilevel converter (MMC) for the purpose of eliminating the time-consuming voltage sorting algorithm and complex voltage balancing regulators. In this paper, the convergence of the DC capacitor voltages within one arm is realized by charging the minimum voltage module and discharging the maximum voltage module during each switching cycle with the assistances of MAX/MIN capacitor voltage detection and PDPWM signals exchanging. The process of voltage balancing control introduces no extra switching commutation, which is helpful in reducing power loss and improving system efficiency. Additionally, the proposed control scheme also possess the merit of a simple executing procedure in application. Simulation and experimental results indicates that the MMC circuit together with the proposed method functions very well in balancing the DC capacitor voltage and improving system efficiency even under transient states.

• Proceedings of the IEEK Conference
• /
• 2004.08c
• /
• pp.667-670
• /
• 2004

In this paper, we have presented the improved IF transceiver architecture and the implementation and experimental results on re-configurable transceiver based on digital IF for multiple wideband OFDM/TDD base stations for high-speed portable internet-service in which is issued Korea. The implemented IF transceiver has been designed to support multiple frequency allocations and multiple standards by only modifying the programmable software not its hardware like as the software-defined-radio concept. Also, the digital complex quadrature modulation technique has been used for the digital IF transmitter, which is able to combine multiple frequency bands in digital processing block not RF block and to reject the image frequency signals. And the bandpass sampling technique has been used for the digital IF receiver to reduce the sampling rate of ADC. This paper has shown the experiment results on the frequency response and constellation on the base-station implemented using the modified IEEE 802.16a/e physical layer channel structure based on OFDM/TDD.

• Journal of the Institute of Electronics Engineers of Korea SP
• /
• v.48 no.6
• /
• pp.124-132
• /
• 2011

In a modern electronic warfare, radar signal environments become more denser and complex. Therefor the capability of reliable signal analysis techniques is required for ES(Electronic warfare Support) system to identify and analysis individual emitter signals from received signals. In this paper, we propose the new signal grouping algorithm to ensure the reliable signal analysis and to reduce the cost of the signal processing steps in the ES. The proposed grouping algorithm uses KDE(Kernel Density Estimator) and its CDF(Cumulative Distribution Function) to compose windows considering the statistical distribution characteristics based on the radar frequency modulation type. Simulation results show the good performance of the proposed technique in the signal grouping.

• 제어로봇시스템학회:학술대회논문집
• /
• 1996.10b
• /
• pp.1352-1355
• /
• 1996

The control of diamond turning is usually achieved through a laser-interferometer feedback of slide position. If the tool post is rigid and the material removal process is relatively static, then such a non-collocated position feedback control scheme may surface. However, as the accuracy requirement gets tighter and desired surface contours become more complex, the need for a direct tool-tip sensing becomes inevitable. The physical constraints of the machining process prohibit any reasonable implementation of a tool-tip motion measurement. It is proposed that the measured force normal to the face of the workpiece can be filtered through an appropriate admittance transfer function to result in the estimated depth of cut. This can be compared to the desired depth of cut to generate the adjustment control action in addition to position feedback control. In this work, the design methodology on the admittance model-based control with a conventional controller is presented. The recursive least-squares algorithm with forgetting factor is proposed to identify the parameters and update the cutting process in real time. The normal cutting forces are measured to identify the cutting dynamics in the real diamond turning process using the precision dynamometer. Based on the parameter estimation of cutting dynamics and the admittance model-based nanodynamic control scheme, simulation results are shown.

• Asian Pacific Journal of Cancer Prevention
• /
• v.14 no.3
• /
• pp.1565-1570
• /
• 2013

Relationships between diet and health have attracted attention for centuries; but links between diet and cancer have been a focus only in recent decades. The consumption of diet-containing carcinogens, including polycyclic aromatic hydrocarbons and heterocyclic amines is most closely correlated with increasing cancer risk. Epidemiological evidence strongly suggests that consumption of dietary phytochemicals found in vegetables and fruit can decrease cancer incidence. Among the various vegetables, broccoli and other cruciferous species appear most closely associated with reduced cancer risk in organs such as the colorectum, lung, prostate and breast. The protecting effects against cancer risk have been attributed, at least partly, due to their comparatively high amounts of glucosinolates, which differentiate them from other vegetables. Glucosinolates, a class of sulphur-containing glycosides, present at substantial amounts in cruciferous vegetables, and their breakdown products such as the isothiocyanates, are believed to be responsible for their health benefits. However, the underlying mechanisms responsible for the chemopreventive effect of these compounds are likely to be manifold, possibly concerning very complex interactions, and thus difficult to fully understand. Therefore, this article provides a brief overview about the mechanism of such compounds involved in modulation of carcinogen metabolising enzyme systems.

• Journal of international Conference on Electrical Machines and Systems
• /
• v.1 no.2
• /
• pp.70-78
• /
• 2012

Due to the variable nature of renewable energy resources and power demand by consumers, it is difficult to operate a power system installed with only one type of renewable energy resource. Grid-based renewable generation may be the only solution to overcome this problem. The conventional approach based on a low-voltage converter with power frequency transformer is commonly employed for grid connection of offshore renewable energy systems. Because of the heavy weight and large size of the transformer, the system can be expensive and complex in terms of installation and maintenance. In this paper, an 11-kV series connected H-bridge (SCHB) multilevel voltage source converter (VSC) is proposed to achieve a compact and light direct grid connection of renewable energy systems. This paper presents the design, simulation and analysis of a five level (5L)-SCHB and an eleven level (11L)-SCHB VSC for 11-kV grid-based renewable energy systems. The performance, cost, modulation scheme and harmonic spectra of the converter are analyzed.

• Animal cells and systems
• /
• v.13 no.1
• /
• pp.9-15
• /
• 2009

Eph receptors and their ephrin ligands have been implicated in a variety of cellular processes such as cellular morphogenesis and motility. Our previous studies demonstrated that Odin, one of the Anks family proteins, functions as a scaffolding protein of the EphA8 signaling pathway leading to modulation of cell migration or axonal outgrowth. Here we show that WDR7 is associated with Odin and that it is possibly implicated in the EphA8 signaling pathway. WD40 repeats present in the COOH-terminal region of WDR7 appear to be crucial for its association with Odin, whereas the binding motif of Odin is located in between ankyrin repeats and PTB domain. Co-immunoprecipitation experiments revealed that association of WDR7 with Odin is enhanced by ephrin ligand treatment, possibly through forming large protein complexes including both EphA8 and ephrin-A5. Consistently, immunofluorescence staining experiments suggested that WDR7 constitute a component of the large protein complexes containing Odin, EphA8 and ephrin-A5. Taken together, our results suggest the WDR7-Odin complexes might be involved in the signaling pathway downstream of the EphA8 receptor.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.35 no.7A
• /
• pp.682-687
• /
• 2010

The IEEE 802.15.4a specification targets the low-rate (LR) Impulse-radio (IR) ultra-wideband (UWB) system which is now widely applied in the WPANs considering rather short distance communications with low complexity and power consumption. The physical (PHY) layer uses concatenated coding with mixed binary phase-shift keying and binary pulse-position modulation (BPSK-BPPM), and direct sequence spreading with time hopping in order that both coherent and non-coherent receiver architectures are supported. In this paper, the performances of multiple access schemes compliant with IEEE 802.15.4a specification are investigated with energy detection receiver, which allow avoiding the complex channel estimation needed by a coherent receiver. However, the performance of energy detection receiver is severely degraded by multi-user interference (MUI), which largely diminishes one of the most fascinating advantages of UWB, namely robustness to MUI as well as the possibility to allow parallel transmissions. So as to improve the performance of multiple access schemes, we propose to apply the novel TH sequences as well as to increase the number of TH positions. The simulation results show that our novel multiple access schemes significantly improve the performance against MUI.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 1996.04a
• /
• pp.49-52
• /
• 1996

The control of diamond turning is usually achieved through a laser-interferometer feedback of slide position. The limitation of this control scheme is that the feedback signal does not account for additional dynamics of the tool post and the material removal process. If the tool post is rigid and the material removal process is relatively static, then such a non-collocated position feedback control scheme may surfice. However, as the accuracy requirement gets tighter and desired surface contours become more complex, the need for a direct tool-tip sensing becomes inevitable. The physical constraints of the machining processprohibit any reasonable implementation of a tool-tip motion measurement. It is proposed that the measured force normalto the face of the workpice can be filterd through an appropriate admittance transfer function to result in the estimated depth of cut. This can be compared to the desired depth of cut to generate the adjustment cotnrol action in addition to position feedback control. In this work, the design methodology on the admittance model-based control with a conventional controller is presented. Based on the empirical data of the cutting dynamics, simulation results are shown.