• Journal of Microbiology
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• v.41 no.3
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• pp.212-218
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• 2003

Citrus phytoene synthase (CitPsy) and ${\beta}$-carotene hydroxylase (CitChx), which are involved in caroteinoid biosynthesis, are distantly related to the corresponding bacterial enzymes from the point of view of amino acid sequence similarity. We investigated these enzyme activities using Pantoea ananatis carotenoid biosynthetic genes and Escherichia coli as a host cell. The genes were cloned into two vector systems controlled by the T7 promoter. SDS-polyacrylamide gel electrophoresis showed that CitPsy and CitChx proteins are normally expressed in E. coli in both soluble and insoluble forms. In vivo complementation using the Pantoea ananatis enzymes and HPLC analysis showed that ${\beta}$-carotene and zeaxanthin were produced in recombinant E. coli, which indicated that the citrus enzymes were functionally expressed in E. coli and assembled into a functional multi-enzyme complex with Pantoea ananatis enzymes. These observed activities well matched the results of other researchers on tomato phytoene synthase and Arabidopsis and pepper ${\beta}$-carotene hydroxylases. Thus, our results suggest that plant carotenoid biosynthetic enzymes can generally complement the bacterial enzymes and could be a means of carotenoid production by molecular breeding and fermentation in bacterial and plant systems.

• Journal of Life Science
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• v.19 no.5
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• pp.561-565
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• 2009

Human Tissue factor is an essential enzyme activator that forms a catalytic complex with factor VII/ VIIa, and catalyzes both the extrinsic and intrinsic blood coagulation cascades. The extracellular domain of human tissue factor is responsible for association with the biological partner. The efficient procedures for preparing biologically active human tissue factor are essential for the preclinical and clinical studies with coaguligands. An expression vector in Escherichia coli has been constructed to direct the production of extracellular human tissue factor without a fusion protein or a $His_6$ at the N-terminus. The recombinant human tissue factor was expressed in large amounts as a non-native state in E. coli. The recombinant protein was simply renatured during the DEAE-sephacel chromatographic purification procedure. Our expression and purification system does not require a protease treatment or an additional chromatographic step to remove a fusion contaminant, which provides a very useful alternative to conventional expression systems for the production of human tissue factor.

• Journal of Educational Research in Mathematics
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• v.25 no.2
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• pp.177-188
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• 2015

In this paper, I investigated the historical development process for the product of two vectors in the plane and space, and draw implications for educational guidance to internal and external product of vectors based on it. The results of the historical analysis show that efforts to define the product of the two line segments having different direction in the plane justified the rules of complex algebraic calculations with its length of the product of their lengths and its direction of the sum of their directions. Also, the efforts to define the product of the two line segments having different direction in three dimensional space led to the introduction of quaternion. In addition, It is founded that the inner product and outer product of vectors was derived from the real part and vector part of multiplication of two quaternions. Based on these results, I claimed that we should review the current deployment method of making inner product and outer product as multiplications that are not related to each other, and suggested one approach for connecting the inner and outer product.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.27 no.4
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• pp.324-333
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• 2005

Distraction osteogenesis for the advancement of hypoplastic maxilla of cleft patients has shown successful results. In this report, rigid external distraction(RED) system and internal distraction device were used for maxillary advancement. Each system has its advantages and disadvantages. Larger amount of advancement can be achieved with RED system. But complex external device may give patients psychological stress. Internal device is invisible. However its distraction amount have limitation for the advancement (< 20mm) and the vector cannot be changed freely during distraction. The authors treated five cleft patients with maxillary hypoplasia(three with RED system and two with internal distractor). Their results were clinically satisfactory. We present the pros and cons of RED and internal system for maxillary distraction osteogenesis.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.35 no.6
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• pp.1413-1420
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• 2015

On the design phase of small size bridge, owing to the lack of related guidelines or standards to determine a superstructure type of bridge, many designers tend to select the type depending on expert's experience and knowledge. Moreover, recently, as types of bridge superstructure become diverse and more conditions need to be considered in the project, the decision makes process become complex. This research covered the selection of a superstructure type of a middle or small size bridge with span length of about 50m, which frequently built for national roadway, selecting type of bridge superstructure more systematic way rather than the existing ways to compare construction methods or to depend on expert's experiences. This study proposes to build a bridge superstructure type selection model using one of the techniques of artificial intelligence techniques SVM by applicability of the model examined through the verification of the actual case.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.38 no.5
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• pp.509-517
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• 2001

Full-search algorithm requires large amount of computation which causes time delay or very complex hardware architecture for real time implementation. In this paper, we propose a fast motion estimator based on bit-plane matching, which reduce the computational complexity and the hardware cost. In the proposed motion estimator, the conventional motion estimation algorithms are applied to the binary images directly extracted from the video sequence. Furthermore, in the proposed VLSI motion estimator, we employ a Pair of processing cores that calculate the motion vector continuously By controlling the data flow in a systolic fashion using the internal shift registers in the processing cores, we avoid using SRAM (local memory) so that we remove the time overhead for accessing the local memory and adopt lower-cost fabrication technology. We modeled and tested the proposed motion estimator in VHDL, and then synthesized the whole system which has been integrated in a 0.6-$\mu$m triple-metal CMOS chip of size 8.15 X 10.84$\textrm{mm}^2$.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.14 no.4
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• pp.1543-1561
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• 2020

Taxonomy of human brain activity is a complicated rather challenging procedure. Due to its multifaceted aspects, including experiment design, stimuli selection and presentation of images other than feature extraction and selection techniques, foster its challenging nature. Although, researchers have focused various methods to create taxonomy of human brain activity, however use of multivariate pattern analysis (MVPA) for image recognition to catalog the human brain activities is scarce. Moreover, experiment design is a complex procedure and selection of image type, color and order is challenging too. Thus, this research bridge the gap by using MVPA to create taxonomy of human brain activity for different categories of images, both colored and gray scale. In this regard, experiment is conducted through EEG testing technique, with feature extraction, selection and classification approaches to collect data from prequalified criteria of 25 graduates of University Technology PETRONAS (UTP). These participants are shown both colored and gray scale images to record accuracy and reaction time. The results showed that colored images produces better end result in terms of accuracy and response time using wavelet transform, t-test and support vector machine. This research resulted that MVPA is a better approach for the analysis of EEG data as more useful information can be extracted from the brain using colored images. This research discusses a detail behavior of human brain based on the color and gray scale images for the specific and unique task. This research contributes to further improve the decoding of human brain with increased accuracy. Besides, such experiment settings can be implemented and contribute to other areas of medical, military, business, lie detection and many others.

• Korean Journal of Applied Biomechanics
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• v.15 no.1
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• pp.45-61
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• 2005

Stance is defined as any state in which the total mass of the body is supported by the feet. In order to maintain stance, the sum of gravito-inertial forces acting on the body must be registered by equal and opposite forces at the region of contact between the organism and the support surface. Balance is controlled by applying forces to the surface of support so as to maintain the body's center of mass vertically above the feet. for a muIti-segment organism, there can be a variety of ways in which balance can be controlled, since movements of different body segments can have similar effects on the control of balance. In general, the organism tends to have a body configuration that is aligned with gravito-inertial force when there are no external forces acting on it. If any segments of the body are not aligned with gravito-inertial force vector, a torque on that segment would tend to move the body's center of mass. The maintenance of postural stability is accomplished in humans by a complex neural control system. This requires organizing integrating and acting upon visual, vestibular, and somatosensory input, providing orientation information to the postural control system. The information necessary to control and coordinate movement is provided by the visual sense of eye position with respect to the surrounding surface layout, the vestibular sense of head orientation in the gravito-inertial space, and the somatic sense of body segment position relative to one another and to the support surface. In this study, perception and action capability was examined from various points of view. The underlying assumption of the study was that the change of postural configuration could be effected by organism, environment and task goal.

• BMB Reports
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• v.34 no.2
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• pp.176-181
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• 2001

Using a retrovirus, foreign genes can be introduced into mammalian cells. The purpose of this study is to produce a retrovirus that can make the infected cells express two genes; the human multidrug resistance gene (MDR1) and the HLA-B7 gene, which is one of the major human histocompatibility complex (MHC) class I genes. For the expression of these genes, the internal ribosome entry site (IRES) was used, which was derived from the encephalomyocarditis (EMC) virus. In order to produce retroviruses, a retroviral vector was transfected into a packaging cell line and the transfected cells were treated with vincristine, which is an anti-cancer drug and a substrate for the MDRI gene product. This study revealed that two genes were incorporated into chromosomes of selected cells and expressed in the same cells. The production of the retrovirus was confirmed by the reverse transcription (RT)-PCR of the viral RNA. The retrovirus that was produced infected mouse fibroblast cells as well as the human U937. This study showed that packaging cells produced the retroviruses, which can infect the target cells. Once the conditions for the high infectivity of retrovirus into human cells are optimized, thus virus will be used to infect hematopoietic stem cells to co-express MDRl and HLA-B7 genes, and develop the lymphocytes that can be used for the immnogene therapy.

• BMB Reports
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• v.44 no.4
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• pp.244-249
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• 2011

The quality of a phage-displayed antibody library deteriorates with clonal variations, which are caused by differentially expressed Escherichia coli antibody genes. Using the human Fab SP114 against the pyruvate dehydrogenase complex-E2 (PDCE2), we created four E. coli TOP10F' clones with a pCMTG phagemid encoding Fab-pIII (pCMTG-Fab), Fd ($V_H+C_{H1}$)-pIII (pCMTG-Fd), or light chain (L) (pCMTG-L), or the vector only (pCMTG-${\Delta}Fab$) to investigate the effect of clonal variations in a defined manner. Compared to the others, the E. coli clone with pCMTG-Fab was growth retarded in liquid culture, but efficiently produced phage progenies by Ex12 helper phage superinfection. Our results suggest that an antibody library must be cultured for a short duration before helper phage superinfection, and that the Ex12 helper phage helped to alleviate the detrimental effect of clonal variation, at least in part, by preferentially increasing functional phage antibodies during phage amplification.