• The Journal of Internal Korean Medicine
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• v.25 no.3
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• pp.461-472
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• 2004

Objectives : For the screening of neuroprotective effects of medicinal herbs, the complex system of animal models suffer some disadvantages in controlling critical parameters such as blood pressure and body temperature. Additionally, application of drugs to the appropriate brain area sometimes is difficult, due to poor permeability though the blood brain barrier, and so potential protective effects might be masked. Methods : Organotypic hippocampal slice culture (OHSC) method has the advantages of being relatively easy to prepare and of maintaining the general structure, including tissue integrity and the connections between cells. Drugs can easily be applied and neuronal damage can easily be quantified by using tissues and culture media. This study demonstrates neuroprotective effects of Puerariae radix (葛根, PR), Salviae miltiorrhizae radix (丹蔘, SR), Rhei rhizoma (大黃, RR), and Bupleuri radix (柴胡, BR). These were screenedand compared to MK-801, antagonist of NMDA receptors, by using OHSC of 1 week-old Sprague-Dawley rats. Oxygen/glucose deprivation (OGD) were conducted in an anaerobic chamber $(85%\;N_2,\;10%\;CO_2\;and\;5%\;H_2)$ in a deoxygenated glucose-free medium for 60 minutes. Water extracts of each herbs were treated to culture media with $5\;{\mu}g/ml$ for 48 hours. Results : Neuronal cell death in the cultures was monitored by densitometric measurements of the cellular uptake of propidium iodide (PI). PI fluorescence images were obtained at 48 hours after the OGD and medicinal herb treatment. Also TUNEL-positive cells in the CAI and DG regions and LDH concentrations in culture media were measured at 48 hours after the OGD. According to measured data, MK-801, PR, SR and BR demonstrated significant neuroprotective effect against excessive neuronal cell death and apoptosis induced by the OGD insult. Especially, PR revealed similar neuroprotective effect to MK-801 and RR demonstrated weak neuroprotective effect. Conclusions : These results suggest that OHSC can be a suitable method for screening of neuroprotective effects of medicinal herbs. (This work was supported by the research program of Dongguk University and Grant 01-PJ9-PG1-01CO03-0003 from Ministry of Health & Welfare.)

• Journal of Life Science
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• v.30 no.6
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• pp.513-521
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• 2020

B-cell-specific Moloney murine leukemia virus integration site 1 (Bmi1) is a polycomb group protein and a core component of polycomb repressive complex 1. Initial research into Bmi1 has focused on its role in tumorigenesis, and it is generally accepted that it is important for the proliferation and survival of cancer cells. However, more recent studies have revealed that Bmi1 is downregulated in brains with neurodegenerative disease and that it regulates the function of mitochondria and reactive oxygen species levels. In this study, we tested the therapeutic potential of Bmi1 in pilocarpine-induced seizures in Bmi1-knockout mice. Bmi1 expression transiently increased in the hippocampal CA1 and CA3 and the dentate gyrus following pilocarpine-induced status epilepticus (SE). In terms of seizure behavior, SE induction was 43.14% and 53.57% for Bmi1^+/+ and Bmi1^+/- mice, respectively. However, there was no significant difference in mortality or hippocampal damage between the two groups. Two months after SE induction, the frequency of epileptic seizures in the Bmi1^+/- mice was 50% lower than in the control group, although the difference was not statistically significant. In addition, mossy fiber outgrowth in the Bmi1^+/- mice was significantly higher than in their wild-type littermates. Taken together, these data indicate that reduced Bmi1 activity increases pilocarpine-induced seizure probability and mossy fiber outgrowth.

• Journal of Life Science
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• v.17 no.8 s.88
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• pp.1147-1151
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• 2007

The human skin is constantly exposed to environmental irritants such as ultraviolet, smoke and chemicals. Free radicals and reactive oxygen species caused by them play critical roles in cellular damage. They not only injure the skin structure but also participate in the immensely complex inflammatory reaction. Anti-wrinkle effects of the Oriental herb extracts(OHE) were evaluated by the determination of anti-oxidation, collagenase inhibition and collagen synthesis in normal human fibroblast. OHE showed antioxidative activity as high as vitamin C, trolox and DL-penicillamine. Also OHE showed promotive effect on collagen synthesis and inhibitory effect on collagenase activity. These results demonstrated that OHE could be useful as an anti-wrinkle cosmetic ingredient.

• Smart Structures and Systems
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• v.10 no.2
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• pp.131-154
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• 2012

In this paper, it is aimed to determine the seismic behaviour of highway bridges by nondestructive testing using ambient vibration measurements. Eynel Highway Bridge which has arch type structural system with a total length of 216 m and located in the Ayvaclk county of Samsun, Turkey is selected as an application. The bridge connects the villages which are separated with Suat U$\breve{g}$urlu Dam Lake. A three dimensional finite element model is first established for a highway bridge using project drawings and an analytical modal analysis is then performed to generate natural frequencies and mode shapes in the three orthogonal directions. The ambient vibration measurements are carried out on the bridge deck under natural excitation such as traffic, human walking and wind loads using Operational Modal Analysis. Sensitive seismic accelerometers are used to collect signals obtained from the experimental tests. To obtain experimental dynamic characteristics, two output-only system identification techniques are employed namely, Enhanced Frequency Domain Decomposition technique in the frequency domain and Stochastic Subspace Identification technique in time domain. Analytical and experimental dynamic characteristic are compared with each other and finite element model of the bridge is updated by changing of boundary conditions to reduce the differences between the results. It is demonstrated that the ambient vibration measurements are enough to identify the most significant modes of highway bridges. After finite element model updating, maximum differences between the natural frequencies are reduced averagely from 23% to 3%. The updated finite element model reflects the dynamic characteristics of the bridge better, and it can be used to predict the dynamic response under complex external forces. It is also helpful for further damage identification and health condition monitoring. Analytical model of the bridge before and after model updating is analyzed using 1992 Erzincan earthquake record to determine the seismic behaviour. It can be seen from the analysis results that displacements increase by the height of bridge columns and along to middle point of the deck and main arches. Bending moments have an increasing trend along to first and last 50 m and have a decreasing trend long to the middle of the main arches.

• Journal of Korean Society of Coastal and Ocean Engineers
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• v.30 no.2
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• pp.69-83
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• 2018

The South China Sea (SCS) is a typical marginal sea characterized with the deep basin, shelf break, shallow shelf, many straits, and complex bathymetry. This study investigated the tidal characteristics and propagation, and reproduced typhoon-induced storm surge in this region using the regional real-time tide-surge model, which was based on the unstructured grid, resolving in detail the region of interest and forced by tide at the open boundary and by wind and air pressure at the surface. Typhoon Haiyan, which occurred in 2013 and caused great damage in the Philippines, was chosen as a case study to simulate typhoon's impact. Amplitudes and phases of four major constituents were reproduced reasonably in general, and the tidal distributions of four constituents were similar to the previous studies. The modelled tide seemed to be within the acceptable levels, considering it was difficult to reproduce the tide in this region based on the previous studies. The free oscillation experiment results described well the feature of tide that the diurnal tide is prevailing in the SCS. The tidal residual current and total energy dissipation were discussed to understand the tidal and sedimentary environments. The storm-surge caused by typhoon Haiyan was reasonably simulated using this modeling system. This study established the regional real-time barotropic tide/water level prediction system for the South China Sea including the seas around the Philippines through the validation of the model and the understanding of tidal characteristics.

• Biomedical Science Letters
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• v.6 no.2
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• pp.119-129
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• 2000

The skin is an organ that has many important roles, including protection against infection, regulation of temperature and fluid loss, and sensory function. Injury to the skin, wound repair normally involves: (1) balanced activity of inflammation, (2) the re-epithelial phase and (3) the matrix formation of remodeling phase. Thus, skin wound healing is a finely controlled biological process involving a series of complex cellular interactions. Laser therapy is being implemented with increasing frequency in medicine. Low intensity laser is one that is capable of producing an energy density so low that any biologic alterations are the result of direct irradiation effect, not thermal events. This study was designed to evaluate the efficacy of low intensity laser therapy on skin wound healing in rabbits. A total of 10 male rabbits (New Zealand White Rabbit), age 8 weeks were used. Skin wound were surgically created dorso-lateral on the flank of 10 rabbits (2$\times$2 cm/damage areas). The experimental animals were treated with 5Hz (830 nm wave length) low-intensity laser (MILTA-01 Model) daily for 10 min (1.6 J/$cm^2$) for 12 days. Control animals were sham treated with the laser head. Laser irradiation animals showed a complete remodeling of the epithelial layer, a positive repair of connective tissues, and enhanced the wound closure rate over time as compared to the control animals. Especially, laser irradiation groups improved fibroblast activity, cellular content, granulation tissue formation, and collagen deposition which is resulted in improving the tensile strength of the wound. These findings suggest that laser photostimulation could accelerate healing of open wound in rabbits, and may be benefit in the treatment of open wound, including decubitis ulcers.

• The Transactions of the Korea Information Processing Society
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• v.6 no.8
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• pp.2233-2244
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• 1999

It is essential to guarantee high reliability of embedded real-time systems since the failure of such systems may result in large financial damage or threaten human life. Though many researches have devoted to fault tolerant mechanisms, most of them are object-level fault tolerant mechanisms that can detect errors occurred in a single object and treat the errors in object-level. As embedded real-time systems become more complex and larger, there exist faults that cannot be detected by or tolerated with object-level fault tolerance. Hence, system-level fault tolerance is needed. System-level fault tolerance examines the status of a system whether the system is normal or not by analyzing the status of objects. When an error is detected it should be capable of locating the fault and performing an appropriate recovery and reconfiguration action. In this paper, we propose RobustRTO(Robust Real-Time Object) that provides object-level fault tolerance capability and RMO(Region Monitor real-time Object) that offers system-level fault tolerance capability. Then we show how highly dependable fault tolerant systems can be modeled by RobustRTO and RMO. The model is presented based on real-time objects.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.8
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• pp.363-368
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• 2016

In safety-critical systems (SCS), failure may result in accidents with serious damage to human beings and property. As systems become more complex and automated, the goal of acquiring safety has attracted increasing attention lately in the defense industry, as well as the rail, automotive, and aerospace industries, among others. As such, the Department of Defense and international organizations have established appropriate standards and guidelines for systems safety and design. To this end, there has been research on the processes, methods, and associated tools for safety design. However, those results do not seem to sufficiently utilize system architectural information. The purpose of this paper is to provide a more systematic approach to SCS design. To better identify potential hazards, design information at each level of system hierarchy is exploited. Based on the results, an integrated process model was developed by combining the processes of system design and safety analysis. As a case study, the resultant integrated process model was applied to the safety design of an automobile system, which shows useful results for safety evaluation.

• Fire Science and Engineering
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• v.31 no.2
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• pp.1-8
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• 2017

A sandwich panel is a composite material composed of a double-sided noncombustible material and insulation core which is used in the inner, outer walls, and roof structure of a building. Despite its excellent insulation performance, light weight and excellent constructability, a flame is brought into the inside of the panel through the joint between the panels, melting the core easily and causing casualties and property damage due to the rapid spread of flame. The current Building Law provides that the combustion performance of finishing materials for buildings should be determined using a fire test on a small amount of specimen and only a product that passes the stipulated performance standard should be used. This law also provides that in the case of finishing materials used for the outer walls of buildings, only materials that secured noncombustible or quasi-noncombustible performance should be used or flame spread prevention (FSP) should be installed. The purpose of this study was to confirm the difference between the dangers of horizontal and vertical fire spread by applying FSP, which is applied to finishing materials used for the outer walls of buildings limitedly to a sandwich panel building. Therefore, the combustion behavior and effects on the sandwich panel according to the application of FSP were measured through the construction to block the spread of flame between the panels using a full scale fire according to the test method specified in ISO 13784-1 and a metallic structure. The construction of FSP on the joint between the panels delayed the spread of flame inside the panels and the flash over time was also delayed, indicating that it could become an important factor for securing the fire safety of a building constructed using complex materials.

• Journal of Korean Society for Geospatial Information Science
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• v.22 no.3
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• pp.145-154
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• 2014

The road network becomes more complex and extensive. Therefore, the inconveniences are caused in accordance with the time delay of the restoration of damaged roads, demands for excessive costs on information collection, and limitations on acquisition of damage information of the roads. Recently, road centric spatial information is gathered using mobile multi sensor system for road inventory. But expensive MMS(Mobile Mapping System) equipments require high maintenance costs from beginning and takes a lot of time in the data processing. So research is needed for continuous maintenance by collecting and displaying the damaged information on a digital map using low cost mobile camera system. In this research we aim to develop the techniques for mosaic with a regular ground sample distance using successive image from oblique camera on a vehicle. For doing this, mosaic image is generated by estimating the homography of high resolution oblique image, and the ground sample distance and appropriate overlap are analyzed using high resolution aerial oblique images which contain resolution target. Based on this we have proposed the appropriate overlap and exposure interval for mobile road inventory system.