• 대한수의사회지
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• 제9권2호
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• pp.23-34
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• 1965

In 1957, a Toxoplasma infection among swine was first discovered in Korea. Thereafter, Complement Fixation Inhibition Test was applied to 2,017 swine serum samples which were obtained from all over the country except Cheju Island. Significant results are

• 대한수의학회지
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• 제12권1호
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• pp.97-119
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• 1972

Studies on modified complement fixation (MCF) test of antiblackleg bovine serum were made and the results obtained were summarized as followings. 1. The most satisfactory antigen for the MCF test among various materials studied was found to be the vegetative cells of Cl, chauvoei grown in cooked meat medium (CMM) containing 0.5mM l-(alpha) alanine and 0.1mM manganese. The antigen was prepared by inoculating the spores of Cl. chauvoei, heated at $70^{\circ}C$. for 30 minutes, into the CMM followed by incubation at $37^{\circ}C$. for 15 hours. 2. An active component contained in the factor serum of fresh normal rabbit serum was found to be C'4 fraction. It was also shown that, furthermore, DEAE cellulose sieved C'4 fraction of the factor serum enhanced antibody titer and the highest antibody titer was resulted by the addition of 0.03 ml, of the factor serum to each tube. 3. More than four fold increases of antibody titer, in antiblbckleg bovine serum-antigen system, was made with the MCF test than that with the direct complement fixation test. 4. The MCF antibody titer of cattle vaccinated against blackleg was 128 until seven month and 64 for five months thereafter.

• 한국동물위생학회지
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• 제20권2호
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• pp.217-223
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• 1997

To investigate infection rates of Anaplasma spp in Red Blood Cell(RBC) of 209 goats and to detect the Anaplasma antibody in sera of 242 goats by Complement Fixation test (CF test), the present study was practiced. The obtained results were as follows : 1. Infection rates of Anaplasma spp in RBC of blood smears, stained by the Giemsa method, were seasonally 15.3% in spring, 20.0% in summer, 39.3% In fall, 16.9% in winter, respectively. 2. The positive rates of the Anaplasma antibody in sera of goats tested by CF test were seasonally 80.2% In spring, 79.0% in summer, 85.7% in fall, 62.0% In winter, respectively.

• 한국동물위생학회지
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• 제30권3호
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• pp.385-391
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• 2007

A novel enzyme linked immunosorbent assay (ELISA) is described and compared with other established serologic tests for bovine brucellosis, namely the rose bengal test (RBT), the complement fixation test (CFT), and the tube agglutination test (TAT) approved and used in Korea. A total of 109 bovine serum samples were tested using all the 4 assays and analyzed as to specificity, sensitivity, reproducibility and predictive value. The ELISA showed 100% agreement with the CFT. The least agreement between ELISA was observed with the TAT. The agreement between the ELISA and RBT was not significantly different from that observed between the CFT and the ELISA. It is concluded that the new assay would be a good candidate for routine serologic survey for brucellosis in Korea. A protocol combining the ELISA and the CFT would increase the power for detection of serologically positive individuals and herds.

• 대한수의사회지
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• 제8권6호
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• pp.56-69
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• 1964

닭의 혈청으로 구성되는 항혈청과 이에 특이한 항원과의 결합물에는 기니픽의 보체가 결합되지 못해서 직접보체결합반응이 불가능하다. 따라서 이 연구에서는 개량보체결합반응으로 아직 실현되지 못한 계두의 보체결합반응을 가능케 하였다. 이 연구에서 얻은 실험성적은 다음과 같다. (1) 계두의 개량보체결합반응에 관한 방법을 발전시켰다. (2) 계두독에 감염된 계태아의 장뇨막으로 만든 항원의 성상을 밝혔다. (3) 계두 및 구두독의 독주별 특이성과 닭에 있어서의 항체형성과정을 밝혔다.

• 대한수의학회지
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• 제6권1호
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• pp.1-9
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• 1966

돈(豚)콜레라는 세계각처(世界各處)에서 널리 유행(流行)되는 돼지의 중요(重要)한 질병(疾病)이다. 그러나 이 질병(疾病)을 혈청학적(血淸學的)으로 진단(診斷)할 수 있는 간단(簡單)하고 고도(高度)로 특이(特異)한 방법(方法)은 아직 없다. 이 연구(硏究)에서는 보체결합반응(補體結合反應)을 이용(利用)한 보다 우수한 혈청적진단법(血淸的診斷法)을 실험(實驗)하여 좋은 성적(成績)을 얻었다. 검출항원(檢出抗原)은 감염돈(感染豚)의 췌장유제(膵臟乳劑)이 양성예(陽性例)에서의 역가(力價)는 1/16 이상이였다. 항혈청(抗血淸)은 조직배양원(組織培養源) 바이러스 재료(材料)를 가토(家兎)에 접종(接種)하여 만들었고 이의 역가(力價)는 1/32 이상이었다. 보체(補體)는 1.1~1.2 충분단위(充分單位)를 사용하였고 감작적혈구(感作赤血球)는 2 단위(單位) 2%로 하였다. 이 방법(方法)으로써 감염돈(感染豚)의 95% 이상이 특이적(特異的)으로 검출(檢出)될 수 있었고, 비감염돈(非感染豚)은 97% 이상이 음성(陰性)으로 검출(檢出)될 수 있었다. 따라서 이 방법(方法)은 돈(豚)콜레라를 진단(診斷)하는데 이용(利用)될 수 있는 보다 낳은 혈청학적(血淸學的) 방법(方法)으로 믿어진다.

• 대한수의학회지
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• 제29권4호
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• pp.517-523
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• 1989

This study was undertaken to establish reliable diagnostic-procedures for the microbiological monitoring of laboratory animals. Murine(mice and rats) antibodies against hemagglutinating virus of Japan(HVJ), mouse hepatitis virus(MHV) and Mycoplasma pulmonis(Mp) were detected sensitively and specifically in experimentally and naturally infected animals' sera by an indirect enzyme-linked immunosorbent assay(ELISA), using urease conjugated antimurine immunoglobulin. The sensitivity and specificity of the complement fixation test which has been apllied widely for serodiagnosis of HVJ, MHV and Mp infections were apparently lower than those of ELISA. From these results, the ELISA was found to be available for the serodiagnosis of HVJ, MHV and Mp infections in mice and rats.

• 대한미생물학회지
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• 제16권1호
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• pp.39-48
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• 1981

보체결합반응은 감염초기의 항체검출에 대한 민감성이 높기 때문에 사람과 동물의 만성질환, 특히 매독, 브루셀라병. 결핵병, �냑鵝뵉퓌鑿�, 클래미디아감염증, 마이코플라스마감염증, 콕시오이드병, 히스토플라스마병 등의 면역학적진단수기와 예방관리를 위한 전염병학적연구목적에 흔히 사용된다. 그러나 검사혈청이 항보체성 또는 친보체성을 갖는 경우는 특이항체를 검출할 수 없는 단점을 갖고 있다. 그러므로 이 연구에서는 친보체성분을 갖는 돼지혈청을 항보체성 사람, 토끼 및 소혈청에 보강하여 특이 항체검출을 위한 민감성과 특이성에 관한 기초실험을 실시하였다. 결핵에 감염된 사람, 토끼 및 소혈청중에 높은 항보체작용이 있는 혈청에 친보체성 돼지혈청을 보강할 때 결핵균(다당체 및 단백질항원)에 대한 특이항체가 검출되었다.

• 대한수의학회지
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• 제27권2호
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• pp.307-316
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• 1987

This study was conducted to identify Babesia sp. strain isolated from the imported beef cattle, Aberdeen-angus heifers, in J farm of Jangsu Prefecture, Cheon-buk Province of Korea, morphologically, etiologically and serologically. Babesia sp. strain was purely isolated through the serial blood passages of three generations against splenectomized calves and one generation of blood passage against non-splenectomized calf(intact calf) by inhibiting the appearance of Theileria sergenti in the blood stream by means of injection of 20% oil pamaquine intramuscularly. In the splenectomized calves, the parasite multiplied markedly in blood stream soon after inoculation and parasitaemia was much severe. An elevated body temperature, anorexia, severe anaemia and icterus were observed clinically. Of three splenectomized calves, two were affected with haemoglobinuria and died. But in the non-splenectomized calf the clinical signs and parasitaemia were very mild. The means of the incubation period, the highest parasitaemia, the highest body temperature and the lowest PCV were 3.5 days, 41.1%, ${42^{\circ}C}$ and 9%, respectively, in the splenectomized calves. In calf erythrocytes Babesia sp. protozoa were polymorphic showing the round, oval, ameboid, piriform and paired piriform etc. The sizes of protozoa were $2.51{\sim}3.91{\times}1.32{\sim}2.19{{\mu}m}$ ($3.20{\times}1.60{\mu}m$). Serologically the isolated Babesia sp. were compared with other parasites, B. ovata, B. bigemina, B. bovis, T. sergenti, A. marginale and A. centrale by using the complement fixation(CF) test. As a result, the antibody titer between the homologous species were high. It was two tubes or more in the CF test. From the results obtained above the pure isolated Babesia species was identical with Babesia ovata Minami and Ishihara, 1980.

• 한국동물위생학회지
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• 제22권2호
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• pp.159-167
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• 1999

This survey was conducted for the serological confirmation on large outbreak of bovine brucellosis in two dairy farms. Serological tests were performed by the plate agglutination test, tube agglutination test, enzyme linked immunosorbent assay(ELISA), complement fixation, ,test(CFT) and rose bengal plate test(RBPT). Total 200 heads(134 heads in farm A and 66 heads in farm B) were tested. The primigravida and positive group have been raised separately in the farm A and both group have been raised together in the farm B.. The result were summarized as follows ; 1. Positive ratios in positive herds of farms by the tube agglutination test were 68.3% in farm A and 53.2% in farm B. 2. Seroconversion to brucella was observed in the primigravidas group in farm B, but was not observed in the primigravidas group in farm A. 3. All calves born in positive herd were serologically negative at time of test. 4. Positive ratio of ELISA in farm A was higher than that of tube agglutination test. 5. Number of positive reactors by the CFT, RBPT in farm A were equal to those of tube agglutination test.