Browse > Article

Comparison of a new ELISA with other serodiagnostic tests for bovine brucellosis  

Hur, Jin (Department of Public Health, College of Veterinary Medicine, Chonbuk National University)
Kakoma, Ibulaimu (Department of Pathobiology, College of Veterinary Medicine, University of Illinois)
Jeong, Jae-Myong (Jeonbuk Institute of Livestock & Veterinary Research)
Lee, Hyeon-Jin (Department of Public Health, College of Veterinary Medicine, Chonbuk National University)
Baek, Byeong-Kirl (Department of Public Health, College of Veterinary Medicine, Chonbuk National University)
Publication Information
Korean Journal of Veterinary Service / v.30, no.3, 2007 , pp. 385-391 More about this Journal
Abstract
A novel enzyme linked immunosorbent assay (ELISA) is described and compared with other established serologic tests for bovine brucellosis, namely the rose bengal test (RBT), the complement fixation test (CFT), and the tube agglutination test (TAT) approved and used in Korea. A total of 109 bovine serum samples were tested using all the 4 assays and analyzed as to specificity, sensitivity, reproducibility and predictive value. The ELISA showed 100% agreement with the CFT. The least agreement between ELISA was observed with the TAT. The agreement between the ELISA and RBT was not significantly different from that observed between the CFT and the ELISA. It is concluded that the new assay would be a good candidate for routine serologic survey for brucellosis in Korea. A protocol combining the ELISA and the CFT would increase the power for detection of serologically positive individuals and herds.
Keywords
Bovine brucellosis; ELISA; Complement fixation test; Rose bengal test; Tube agglutination test;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Ariza J, Servitje O, Pallares R, et al. 1989. Characteristic cutaneous lesions in patients with brucellosis. Arch Dermatol 125:380-383   DOI
2 Quinn PJ, Carter ME, Markey B, et al. 1994. Clinical Veterinary Microbiology. Wolfe Press:261-267
3 임윤규, 양기천, 이경갑, 등. 1995. SDS 처리한 브루셀라 항원과 Yersinia enterocolitica O:9주의 혈청학적 교차반응 연구. 대한수의학회지 35(1):143-148
4 Godfroid J, Saegerman C, Wellemans V, et al. 2002. How to substantiate eradication of bovine brucellosis when aspecific serological reactions occur in the course of brucellosis testing. Vet Microbiol 90:461-477   DOI   ScienceOn
5 Nielsen K. 2002. Diagnosis of brucellosis by serology. Vet Microbiol 90:447-459   DOI   ScienceOn
6 Lambert G, Amerault TE. 1962. Comparative study of three serological tests for detecting the response in cattle to virulent Brucella abortus. Am J Vet Res 23:529-537
7 Tekkoke KIH, Berker M, Ozcan et al. 1993. Brucellosis of the spine. Neurosurgery 33:838-844
8 박동권, 이창희. 1959. 우리나라에 발생한 축우 Brucella 증에 대하여. 수의계 3:192-195
9 Berger TG, Guill MA, Goette DK. 1981. Cutaneous lesions in brucellosis. Arch Dermatol 117:40-42   DOI
10 Morgan WJB, MacKinnon DJ, Lawson JR, et al. 1969. The rose bengal plate agglutination test in the diagnosis of brucellosis. Vet Rec 85 : 636-641   DOI
11 Wright PF, Tounkara K, Lelenta M et al. 1997. International reference Standards: antibody standards for the indirect enzyme-linked immunosorbent assay. Rev Sci Tech 3 : 824-832
12 Nielson K, Kelly L, Gall D, et al. 1996. Comparison of enzyme immunoassays for the diagnosis of bovine brucellosis. Prev Vet Med 26:17-32   DOI   ScienceOn
13 손준용, 이길웅, 유재창 등. 1986. Zoonosis 부루셀라증에 관한 연구. 국립보건원보 23:281-295
14 Wright PF, Nilsson E, Van Rooij EMA, et al. 1993. Standardization and validation of enzyme-linked immunosorbent assay techniques for the detection of antibody in infectious disease diagnosis. Rev Sci Tech Off Int Epiz 12:435-450   DOI
15 Alton GG, Jones LM, Angus RD, et al. 1988. Techniques for the Brucellosis laboratory. Institut National de la Recherche Agronomique. Paris
16 김금화, 안수환, 박용호 등. 1982. 부루셀라병 검색에 사용되는 여러가지 혈청진단법의 비교 연구. 대한수의학회지 22(2): 149-153
17 Flores-Castro R, Carmichael LE. 1980. Canine brucellosis. Curr Vet Ther 7:1303-1305
18 Vallat B, 2004. Manual of diagnostic tests and vaccines for terrestrial animals (mammals, birds and bees). 5 eds. Office International Des Epizooties. Paris:409-438
19 정석찬, 조동희, 남향미 등. 2007. 국내 소 Brucella병의 발생 및 연구동향. 한국수의공중보건학회지 31(2) : 91-103