• Title/Summary/Keyword: Compartment

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Electrochemical Control of Metabolic Flux of Weissella kimchii sk10: Neutral Red Immobilized in Cytoplasmic Membrane as Electron Channel

  • PARK, SUN-MI;KANG, HYE-SUN;PARK, DAE-WON;PARK, DOO-HYUN
    • Journal of Microbiology and Biotechnology
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    • v.15 no.1
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    • pp.80-85
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    • 2005
  • Electrochemical control of the metabolic flux of W. kimchii sk10 on glucose and pyruvate was studied. The growing cell of W. kimchii sk10 produced 87.4 mM lactate, 69.3 mM ethanol, and 4.9mM lactate from 83.1mM glucose under oxidation condition of the anode compartment, but 98.9 mM lactate, 84.3mM ethanol, and 0.2 mM acetate were produced from 90.8 mM glucose under reduction condition of the cathode compartment for 24 h, respectively. The resting cell of W. kimchii sk10 produced 15.9 mM lactate and 15.2 mM acetate from 32.1 mM pyruvate under oxidation condition of the anode compartment, and 71.3 mM lactate and 3.8 mM acetate from 79.8mM pyruvate under reduction condition of the cathode compartment. The redox balance (NADH/$NAD^+$) of metabolites electrochemically produced from pyruvate was 1.05 and 18.76 under oxidation and reduction conditions, respectively. On the basis of these results, we suggest that the neutral red (NR) immobilized in bacterial membrane can function as an electron channel for the electron transfer between electrode and cytoplasm without dissipation of membrane potential, and that the bacterial fermentation of W. kimchii sk10 can be shifted to oxidized or reduced pathways by the electrochemical oxidation or reduction, respectively.

Kinetic Analysis of the Hepatic Transport of Organic Anions: Role of Intracellular Cytoskeleton (유기 음이온계 약물의 간내 이행과정에 있어서 Cytoskeleton의 역할에 관한 속도론적 연구)

  • Chung, Youn-Bok;Han, Kun;Yuk, Dong-Yeon
    • Journal of Pharmaceutical Investigation
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    • v.22 no.1
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    • pp.11-21
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    • 1992
  • The effects of colchicine on the plasma elimination and biliary excretion of various organic anions in rats were examined. Elimination of indocyanine green (ICG) or rose bengal (RB) from plasma was significantly delayed when rats were treated with colchicine (3 mg/kg body weight) 3 hr prior to the administration of the dye. On the other hand, disappearance of sulfobromophthalein (BSP) or bromophenol blue (BPB) from plasma was not influenced by colchicine. The plasma disappearance and biliary excretion of organic anions were kinetically analyzed based on a compartment model, in which the deep compartment and the unknown disposition are incorporated. The transfer rate constants of ICG or RB, $k_{23}$ (from the liver to the deep compartment) and $k_{3B}$ (from the deep compartment to the bile), were decreased by colchicine, but those of BSP or BPB were not changed. A mechanism for the decrease in the $k_{23}$ and $k_{3B}$ values for ICG and RB might be explained by a inhibition of colchicine to the intracellular cytoskeleton. The hepatocellular distribution of RB or BPB was then determined. BPB mainly distributed to the cytosolic fraction, but RB distributed to each hepatocyte organelle. Taken together. it was suggested that ICG or RB is transported through hepatocytes into bile with the aid of the cytoskeleton, whereas BSP or BPB is handled by hepatocytes in a different way.

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Effects of the Geometry and Location of an Vertical Opening on the Fire Characteristics in the Under-Ventilated Compartment Fire (환기부족 구획화재에서 수직 개구부의 형상 및 위치가 화재특성에 미치는 영향)

  • Mun, Sun-Yeo;Park, Chung-Hwa;Hwang, Cheol-Hong;Park, Seul-Hyun
    • Fire Science and Engineering
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    • v.27 no.3
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    • pp.20-29
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    • 2013
  • To investigate numerically the effects of geometry and location of vertical opening on the thermal and chemical fire characteristics in full-scale under-ventilated compartment fires, the ventilation factor ($A\sqrt{h}$) to estimate a theoretical maximum inflow of ambient air and the mass loss rate in a heptane pool fire were fixed for all cases. It was shown that variations in door geometry affected significantly the change in thermal and chemical characteristics inside the compartment. Variations in window location resulted in the complex change in additional fire characteristics including the fire duration time and recirculating flow structure. These results were analyzed in details by the multi-dimensional flow and fire characteristics including the vent flow and fuel/air mixing phenomena.

Evaluation of Design Fire Curves for Gas Fires in a Compartment Using CFAST (CFAST를 이용한 구획실 가스화재의 디자인 화재곡선 평가)

  • Baek, Bitna;Oh, Chang Bo;Hwang, Cheol-Hong
    • Fire Science and Engineering
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    • v.32 no.4
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    • pp.7-16
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    • 2018
  • In this study, the prediction performance of design fire curves (DF) was evaluated for gas fires in a compartment by using CFAST. The CFAST simulations adopted the 2-stage DF suggested by the previous study and the Quadratic and Exponential DF suggested by Ingason. It was found by comparing the simulation and experimental results that the overall prediction performance of the design fire cures for the spatially-averaged temperature and concentrations of $O_2$ and $CO_2$ was, from the most reasonable to the most inaccurate, 2-stage DF > Quadratic DF > Exponential DF. The CFAST simulation could not predict for the difference in the spatially-averaged temperature and concentrations of $O_2$ and $CO_2$ at door and inner side locations in a compartment. The CFAST simulations also showed a limitation in the prediction of the spatially-averaged temperature at lower layer and the concentration of CO.

Modeling Study on Nuclide Transport in Ocean - an Ocean Compartment Model (해양에서의 핵종이동 모델링 - 해양구획 모델)

  • Lee, Youn-Myoung;Suh, Kyung-Suk;Han, Kyong-Won
    • Nuclear Engineering and Technology
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    • v.23 no.4
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    • pp.387-400
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    • 1991
  • An ocean compartment model simulating transport of nuclides by advection due to ocean circulation and intertaction with suspended sediments is developed, by which concentration breakthrough curves of nuclides can be calculated as a function of time. Dividing ocean into arbitrary number of characteristic compartments and performing a balance of mass of nuclides in each ocean compartment, the governing equation for the concentration in the ocean is obtained and a solution by the numerical integration is obtained. The integration method is specially useful for general stiff systems. For transfer coefficients describing advective transport between adjacent compartments by ocean circulation, the ocean turnover time is calculated by a two-dimensional numerical ocean model. To exemplify the compartment model, a reference case calculation for breakthrough curves of three nuclides in low-level radioactive wastes, Tc-99, Cs-137, and Pu-238 released from hypothetical repository under the seabed is carried out with five ocean compartments. Sensitivity analysis studies for some parameters to the concentration breakthrough curves are also made, which indicates that parameters such as ocean turnover time and ocean water volume of compartments have an important effect on the breakthrough curves.

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Assessment of Thyroid Dose Evaluation Method by Monitoring of I-131 Concentration in Air (공기중 I-131 농도 감시에 의한 갑상선 피폭 평가법의 적용성)

  • Lee, Jong-Il;Seo, Kyung-Won
    • Journal of Radiation Protection and Research
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    • v.19 no.1
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    • pp.69-80
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    • 1994
  • The TCMI(Three-Compartment Model for iodine) computer code has been developed, which is based on the three-compartment model and the respiratory model recommended in ICRP publication 54. This code is able to evaluate the thyroid burden, dose equivalent, committed dose equivalent and urinary excretion rate as time-dependent functions from the input data: working time and the radioiodine concentration in air. Using the TCMI code, the time-dependent thyroid burdens, the thyroid doses and the urinary excretion rates were calculated for three specific exposure patterns : acute, chronic and periodic. Applicability as an internal dose evaluation method has been assessed by comparing the results with some operational experiences. Simple equations and tables are provided to be used in the evaluation of the thyroid burden and the resulting doses for given I-131 concentration in air and the working time.

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A Validation Study of Temperature Field Predicted by Computational Fire Model for Spray Fire in a Multi-Compartment (다중구획공간내 분무화재시 화재해석모델의 온도장 검증연구)

  • Kim, Sugn-Chan
    • Fire Science and Engineering
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    • v.28 no.5
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    • pp.23-29
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    • 2014
  • The present study has been conducted to investigate the validity of the computational fire model and the results predicted by BRANZFIRE zone model and FDS field model are compared with a real scale fire test with spray fire in a multi-compartment. The liquid spray fires fueled with toluene and methanol are used as the fire source and the quantitative measurement of heat release rate is performed in an isolated ISO-9705 compartment with a standard door opening. The temperature field predicted by FDS model showed good agreement with the measurement in the fire room and the corridor, and BRANZFIRE model also gave acceptable result in spite of its simplicity and roughness. The mean temperature predicted by FDS model corresponds with measurement within maximum discrepancy range of 25% and the overall mean value of FDS model matched well with experimental data less than 10%. This study can contribute to establish the limitation and application scope of computational fire model and provide reference data for applying to reliable fire risk assessment.

A Experimental Study on Window Glass Breakage in Compartment Fires (구획 화재시 창유리 파괴 현상에 관한 실험적 연구)

  • 이수경;김종훈;최종운;이정훈
    • Fire Science and Engineering
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    • v.12 no.3
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    • pp.21-30
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    • 1998
  • This is a study on the relation of window glass breaking time, shape, and vent condition in a compartment fire through the experiment. We recognize the phenomenon that window glass breakage in a compartment fire be arose from the thermal stresses due to the temperature temperature difference was 233.4$^{\circ}C$ for test 1-3, 138$^{\circ}C$ for text 2-1, 83.6$^{\circ}C$ for test 2-2. The interior test 2-2, 400.9$^{\circ}C$ for test 2-3. so if the flame didn't reach at the surface of window glass, the breakage of glass occure at 40$0^{\circ}C$~50$0^{\circ}C$. when the fire size reached to 1541.14kW, the window might be broken by thermal stress. But window glass was not collapsed.

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Early Lateral Compartment Physeal Closure of the Elbow in Osteochondritis Dissecans of the Adolescent Baseball Players (청소년기 야구 선수의 박리성 골연골염에서 주관절 외측 구획 성장판의 조기 폐쇄)

  • Ku, Jung Hoei;Cho, Hyung Lae;Park, Ki Bong;Lee, Wan Seok
    • The Korean journal of sports medicine
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    • v.36 no.4
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    • pp.180-188
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    • 2018
  • Purpose: The purpose of this study is to identify bilateral differences of physeal closure of the lateral compartment of the elbow in osteochondritis dissecans (OCD) and related factors with premature physeal closure. Methods: Initial radiographs of the bilateral elbows in 40 baseball players with OCD (group I) were reviewed for the status of physeal closure of the lateral compartment; capitellum, radial head, lateral epicondyle. Forty baseball players with medial epicondylar apophysitis (group II) were enrolled as a control. Relative status of physeal closure of dominant elbow was defined as early, same, and delayed. Bilateral differences of the status of physeal closure were analyzed between groups, and according to the radiographic stages, extent of the lesions and demographic factors in group I. Results: Significant early physeal closures of dominant elbows were identified in group I in capitellum (group I, 55%; group II, 3%), radial head (group I, 53%; group II, 3%), and lateral epicondyle (group I 37%; group II, 5%). In group I, advanced stage and extended lesion showed early lateral compartment physeal closure especially in capitellum and radial head, and players with longer career length and limitation of motion showed early closure. Conclusion: Over the half of the adolescent baseball players with OCD demonstrated early radiocapitellar physeal closures of dominant elbow in initial presentation. Because premature physeal closure contributes to the development of arthritis without appropriate radiocapitellar remodeling, early detection of OCD is essential for prevention of arthritis and successful conservative management.

Comparison of Vibration Characteristics of Cargo Bikes on General Roads and Bicycle Roads (일반도로와 자전거도로에서의 카고바이크 화물 적재함 진동 특성 비교)

  • Dong Yul Kim;Myenog Guk Yu;Heng Suk Lee;Sang Min Kim
    • KOREAN JOURNAL OF PACKAGING SCIENCE & TECHNOLOGY
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    • v.29 no.3
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    • pp.175-179
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    • 2023
  • This paper analyzes the vibration characteristics within the cargo compartment of a three-wheeled cargo bike when used on both regular roads and dedicated bicycle lanes. When cargo is loaded into the cargo compartment of a cargo bike and driven on the road, the shocks and vibrations transmitted from the ground can potentially affect the transported goods and even lead to product damage. As the vibration characteristics applied to the cargo compartment may vary depending on the condition of the road, vibration sensors were attached to the cargo bike's cargo compartment for data collection during operation on different road types. According to KS T ISO 13355 standards, the cargo bike can withstand vibrations within the range of 10 Hz to 60 Hz when operating on both bicycle lanes and regular roads. However, it is observed that there are peaks exceeding the profile in the frequency range of 3-6 Hz. In the 70-200 Hz range, the profile is exceeded on both regular roads and bicycle lanes, with a tendency for higher exceedance on bicycle lanes. The Grms value within the frequency range of KS T ISO 13355 is 5.926 m/s2 (0.604 Grms). When operating on bicycle lanes with cargo, the Peak envelope optimization PSD value is 6.553 Grms, while on bicycle lanes, it is 7.708 Grms, indicating a difference of at least tenfold.