For large and rapid screening of high-yielding mutants of lovastatin produced by filamentous fungal cells of Aspergillus terreus, one of the most important stage is to test as large amounts of mutated strains as possible. For this purpose, we intended to develop a miniaturized cultivation method using $7m{\ell}$ culture tube instead of traditional $250m{\ell}$ flask (working volume $50m{\ell}$). For obtaining large amounts of conidiospores to be used as inoculums for miniaturized cultures, 4 components i.e., glucose, sucrose, yeast extract and $KH_2PO_4$ were intensively investigated, which had been observed to show positive effect on enhancement of spore production through Plackett-Burman design experimet. When optimum concentrations of these components that were determined through application of response surface method (RSM) based on central composite design (CCD) were used, maximum spore numbers amounting to $1.9\times10^{10}$ spores/plate were obtained, resulting in approximately 190 fold increase as compared to the commonly used PDA sporulation medium. Using the miniaturized cultures, intensive strain development programs were carried out for screening of lovastatin high-yielding as well as highly reproducible mutants. It was observed that, for maximum production of lovastatin, the producers should be activated through 'PaB' adaptation process during the early solid culture stage. In addition, they should be proliferated in condensed filamentous forms in miniaturized growth cultures, so that optimum amounts of highly active cells could be transferred to the production culture-tube as reproducible inoculums. Under these highly controlled fermentation conditions, compact-pelleted morphology of optimum size (less than 1 mm in diameter) was successfully induced in the miniaturized production cultures, which proved essential for maximal utilization of the producers' physiology leading to significantly enhanced production of lovastatin. As a result of continuous screening in the miniaturized cultures, lovastatin production levels of the 81% of the daughter cells derived from the high-yielding producers turned out to be in the range of 80%$\sim$120% of the lovastatin production level of the parallel flask cultures. These results demonstrate that the miniaturized cultivation method developed in this study is efficient high throughput system for large and rapid screening of highly stable and productive strains.
The sinus tympani is subject to great variability in the size, shape and posterior extent. A heavy compact bony zone, especially in the posterior portion and the narrow space between the facial nerve and posterior semicircular canal are the limitation of surgical approach. The facial recess should be opened, creating a wide connection between the mesotympanum and mastoid in the Intact canal wall tympanoplasty with mastoidectomy. The surgically created limits of the facial recess are the facial nerve medially, the chorda tympani laterally and the bone adjacent to the incus superiorly. Using adult Korean's thirty-five temporal bones, the authors measured the osteologic reslationship in the posterior tympanum, especially sinus tympani and facial recess. The result was as followed. 1. The average distance from the anterior end of the pyramidal eminence. 1) to the edge of the sinus tympani directly posterior was 2.54(1.05-5.40)mm. 2) to the maximum posterior extent was 3.22(1.25-7.45)mm. 3) to the maximum cephaled extent was 0.67 (0.40-1.75)mm. 2. The boundary of the sinus tympani was 82.9% from the lower margin oval window to the upper margin round window niche. 3. The deepest part of the sinus tympani was 62.9% in the mid portion, between the ponticulus and subiculum. 4. The oblique dimension from the fossa incudis above to the hypotympanum below was 8.13(7.90-9.55)mm. 5. The transverse dimensions midway between the oval window above and round window below was 3.00(2.85-3.45)mm. 6. The transverse dimension at the level of the fossa incudis was 1.81(1.40-2.15)mm. 7. The facial nerve dehiscence was 14.3%. 8. Anterior-posterior diameter of the footplate was 2.98(2.85-3.05) mm. 9. The average distance from the footplate. 1) to the cochleariform process was 1.42(1.35-1.55) mm. 2) to the round window niche was 1.85(1.45-2.10) mm.
Lee, Jin Wook;Chung, Seok Woo;Lee, Seung Jong;Jung, Woohyun;Byun, Yong Soo;Hwang, Sang Yeon;Jeon, Dong Hwan;Ryu, Sang Oh;Lee, Ji Eun;Jeong, Ki Jin;Kim, Jin Ho;Yun, Yongseung
Korean Chemical Engineering Research
/
v.52
no.5
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pp.657-666
/
2014
Coal gasification technology is considered as next generation clean coal technology even though it uses coal as fuel which releases huge amount of greenhouse gas because it has many advantages for carbon capture. Coal or pet-coke slurry gasification is very attractive technology at present and in the future because of its low construction cost and flexibility of slurry feeding system in spite of lower efficiency compared to dry feeding technology. In this study, we carried out gasification experiment using bituminous coal slurry sample by integrating coal slurry feeding facility and slurry burner into existing dry feeding compact gasifier. Especially, our experiment was conducted under fairly lower operation temperature than that of existing entrained-bed gasifier, resulting in partial slagging operation mode in which only part of ash was converted to slag and the rest of ash was released as fly ash. Carbon conversion rate was calculated from data analysis of collected slag and ash, and then cold gas efficiency, which is the most important indicator of gasifier performance, was estimated by carbon mass balance method. Fairly high performance considering pilot-scale experiment, 98.5% of carbon conversion and 60.4% of cold gas efficiency, was achieved. In addition, soundness of experimental result was verified from the comparison with chemical equilibrium composition and energy balance calculations.
This study is intended to find out the degree of compaction in field compaction, with soil type, the thickness of soil layer and the number of roller passing through the field density test and the field C. B. R. test by comparing vibration and non-vibration compactions. The results in this study are summarized as follows. 1. When the number of roller passing is few, it shows that the efficiency of the compaction by vibratory compaction is greater than that by non-vibratory compaction, the difference of the compaction ratio between vibratory and non-vibratory compaction is decreased according as the number of roller passing is more frequent. 2. Mechanizing on a large scale it is possible for a large equipment to be able to reach the point of A-1 compact ion method with three to five times of roller passing. To provide for mechanizing on a larger scale it is advisable to fix the standard by the D-2 compaction method. 3. As dry-density increases, the C. B. R. value increases, but the increasing ratio of C. B. R. value showed greater in vibrating compacting. 4. According as the number of roller passing increases, the increasing tendency of the C. B. R. Value is slow and the difference of the C. B. R. value between vibration and non-vibration compaction is large, the C. B. R. value showed greater about 20% in vibration compaction than in non-vibration compaction. 5. In C-5 soil type, with increasing the thickness of compaction, the degree of compaction is decreased. When the thickness of compaction is increased from 20cm to 30cm, the degree of compaction is decreased slowly, while the thickness of the compaction is increased from 30cm to 40cm the degree of compaction is decreased remarkably. Therefore it is advisable to compact the ground under the thickness of 30cm.
To develope a technique for efficiently managing fertilizer for cucumber, a quick test method to quantify nitrate content in soil solution and leaf petiole juice using a simple instrument was investigated. Among the nitrate analyzing instruments such as compact ion meter, nitrate ion meter, and test strip with reflectometer, the paper test-strip used in conjunction with a hand-held reflectometer was most closely correlated with ion chromatography method in nitrate content, and then it would be suggested with a tool that a farmer can use rapidly, conveniently and accurately for nitrate analysis in a field. Nitrate content in soil solution collected by porous cup was very variable on the lapsed time after drip irrigation and the sampling positions such as soil depth and the distance from dripper. As a result, a significant correlation between nitrate contents of soil solutions and 2M KCl soil extract was not found. However, nitrate content in soil solution extracted with a volume basis (soil:water=1:2) showed the highly significant correlation with that in 2M KCl extract. Nitrate contents of cucumber leaf petiole juices was greatly different between upper and lower leaves. Eleven to sixteen positioned-leaf would be a proper sampling position to determine nitrate content in leaf petiole for evaluating nutrient state by plant tissue analysis. From the secondary regression equations between nitrate contents of soil and petiole juice and the yield of cucumber, nitrate levels for real time diagnosis were estimated as $400mg\;l^{-1}$ soil solution by porous cup. $300mg\;l^{-1}$ in a soil volume extraction, and $1400mg\;l^{-1}$ in petiole juice from spring to summer season. In addition, the maximum yield of cucumber fruit in pot test was obtained in nitrate $1500mg\;l^{-1}$ level of petiole juice, which was similar to nitrate $1400mg\;l^{-1}$ in greenhouse trial.
This study was carried out to investigate the effects of uniconazole treatment on the growth and flowering of potted Chrysanthemum indicum L. for high quality pot plant production. Uniconazole was drenched at 0.05, 0.01, or 0.15 mg a.i./pot at 14 days after planting (DAP) of rooted cuttings. Simultaneously the short-day treatment (SDT) and pinching were adapted. The same amount of uniconazole (0.05 mg a.i./pot) was spilt drenched at once, twice, and three times, respectively, at 1 week interval. Uniconazole markedly reduced plant height, branch length, and stem diameter. Plant height was reduced linearly with increasing uniconazole concentration at 0.05, 0.01, or 0.15 mg a.i./pot up-to 41.6%, 52.5%, and 58.5%, respectively. In 0.05 mg a.i./pot, the number of branches greatly increased and plant height of 22.6 cm was adequate for pot plant. However, higher concentrations (0.10, 0.15 mg a.i.) were not suitable for production of high quality pot plant (17.0, 14.8 cm, respectively). Pinching and SDT decreased the number of days to visible bud, while uniconazole treatments delayed days to visible bud by 5-9 days compared with pinching and SDT. Number of visible buds was highest at 0.05 mg a.i./pot uniconazole treatment. However, flower diameter was decreased by uniconazole treatment, resulting in compact form. Number of stomata was increased by uniconazole treatment. The length of vascular tissues of uniconazole-treated plants ($11.2{\mu}m$) was smaller than that of non-treated plants ($15.0{\mu}m$, and the size of xylem vessel was also decreased. Uniconazole treatment at 0.05 mg a.i./pot at 14 DAP with pinching and SDT were recommended for pot plant production of C. indicum L.
Transit Oriented Development(TOD) has been the dominant paradigm for urban development. The traditional approach to the development of urban rail station areas demonstrates apparent weaknesses when it comes to compact and mixed-use types of development for these stations. Thus, the objective of this study is to derive evaluation indicators with which to assess urban rail station areas using TOD planning elements. A focus group interview was conducted to explore TOD planning elements. In order to examine the level of TOD to be achieved, an AHP technique was utilized to analyze the importance of several variables. Z-scores and Re-scores were applied to derive the values for the stations. The Variables related to the land use type, mixed use, floor area, and public transit were found to be the major contributing factors to the achievement of TOD in urban rail station areas. The results of this study show that the level of TOD achievement varies according to the major land use, density, accessibility, and mixed-use planning elements.
Personal Rapid Transit (PRT) has emerged as a promising alternative transportation mode for transit-oriented sustainable communities by creating compact and walkable environments with competitive construction and operational costs. This study seeks to capture the changes in travel mode choice behavior in response to the introduction of PRT to travelers who have no previous experience of using it. A critical issue in modeling the PRT mode choice is how to capture travelers' perception and evaluation of the unexperienced travel mode. The data used come from questionnaire surveys, in which RP (Revealed Preference) and SP (Stated Preference) data were collected in relation to travel mode choices with and without PRT systems. The questionnaire was designed especially for mitigating the potential bias in favor of or against choosing PRT. In addition, an efficient approach was proposed to reduce the number of SP questions by avoiding the complex fractional factorial design which tends to make it difficult for respondents to keep their attention throughout the survey. The analysis results show that the proposed approach is able to realistically capture the effects of explanatory variables on the travel mode choice. Discrete choice models are developed to predict travelers' mode choices under different choice scenarios by varying PRT system specifications and operational characteristics. PRT patronages are projected for two different test sites using the developed PRT mode choice models.
Journal of the Korean Association of Geographic Information Studies
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v.19
no.4
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pp.1-16
/
2016
After entering a more stable growth phase since the 2000s, new urban development has become smaller and fragmented and interiors of existing cities are aging and becoming underutilized. Therefore, using vacant and underutilized land in urban area has high value in national urban policies in terms of compact urban development and circular land use management. To do so, it is necessary to develop scientific research methods and tools to better understand the characteristics and potential of vacant and underutilized land. The main goals of the study are as follows. First, this research aims to develop a pilot system for analysis and assessment of abandoned, vacant, and underutilized space. The pilot system will act as a scientific policy support measure to systematically identify vacant and underutilized space. Busan Metropolitan City, with a variety of vacant and underutilized space, was selected as the case study area. The second goal is to develop a planning support system for abandoned, vacant and underutilized Land using open source GIS software, uDig. GIS based decision support systems or planning support systems to resolve spatial problem is not a new innovation, but using open source GIS provides new opportunities and potential. To conclude, the research will derive implications for future work.
The Journal of Korean Institute of Electromagnetic Engineering and Science
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v.17
no.9
s.112
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pp.811-820
/
2006
Ultra wideband CPW batons are proposed in this paper. The proposed talons are consisted of ultra wideband multi-stage Wilkinson dividers and 'X'-shaped $180^{\circ}$ out-of-phase generator. Bottom-bridges and via-holes are used to connect CPW ground lines instead of the conventional air-bridges which require troublesome manual working in fabrication with HMIC(Hybrid Microwave Integrated Circuits) substrates. The proposed CPW batons have ultra wideband of 3 or $10(=F_{figh}/F_{low})$ theoretically, the wideband characteristics and S-parameters of the basis Wilkinson divider are directly converted to those of the proposed batons. The proposed batons are so compact and small compared to the conventional Wilkinson batons because no additional area for out-of-phase section is required. So the size of the proposed batons is exactly the same as that of the basis Wilkinson dividers. As examples, 3-stage and 7-stage wideband Wilkinson dividers are converted to the proposed batons. Their measured operating bandwidth are $1\sim3GHz$ and $0.8\sim5GHz$, respectively, with excellent matching, isolation and power division performances. The measured magnitude and phase balance errors are ${\pm}0.5dB\;and\;0.45\;dB,\;and\;{\pm}5^{\circ}\;and\;{\pm}10^{\circ}C$ over $1\sim3GHz\;and\;0.8\sim5GHz$, respectively.
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