• Title/Summary/Keyword: Community Identification

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Genetic and Phenotypic Diversity of Plant Growth Promoting Rhizobacteria Isolated from Sugarcane Plants Growing in Pakistan

  • Mehnaz, Samina;Baig, Deeba N.;Lazarovits, George
    • Journal of Microbiology and Biotechnology
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    • v.20 no.12
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    • pp.1614-1623
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    • 2010
  • Bacteria were isolated from roots of sugarcane varieties grown in the fields of Punjab. They were identified by using API20E/NE bacterial identification kits and from sequences of 16S rRNA and amplicons of the cpn60 gene. The majority of bacteria were found to belong to the genera of Enterobacter, Pseudomonas, and Klebsiella, but members of genera Azospirillum, Rhizobium, Rahnella, Delftia, Caulobacter, Pannonibacter, Xanthomonas, and Stenotrophomonas were also found. The community, however, was dominated by members of the Pseudomonadaceae and Enterobacteriaceae, as representatives of these genera were found in samples from every variety and location examined. All isolates were tested for the presence of five enzymes and seven factors known to be associated with plant growth promotion. Ten isolates showed lipase activity and eight were positive for protease activity. Cellulase, chitinase, and pectinase were not detected in any strain. Nine strains showed nitrogen fixing ability (acetylene reduction assay) and 26 were capable of solubilizing phosphate. In the presence of 100 mg/l tryptophan, all strains except one produced indole acetic acid in the growth medium. All isolates were positive for ACC deaminase activity. Six strains produced homoserine lactones and three produced HCN and hexamate type siderophores. One isolate was capable of inhibiting the growth of 24 pathogenic fungal strains of Colletotrichum, Fusarium, Pythium, and Rhizoctonia spp. In tests of their abilities to grow under a range of temperature, pH, and NaCl concentrations, all isolates grew well on plates with 3% NaCl and most of them grew well at 4 to $41^{\circ}C$ and at pH 11.

Identification of Risky Subgroups with Sleep Problems Among Adult Cancer Survivors Using Decision-tree Analyses: Based on the Korean National Health and Nutrition Examination Survey from 2013 to 2016 (의사결정나무 분석을 이용한 성인 암경험자의 문제수면 위험군 예측: 2013-2016년도 국민건강영양조사 자료 분석)

  • Kim, Hee Sun;Jeong, Seok Hee;Park, Sook Kyoung
    • Journal of Korean Biological Nursing Science
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    • v.20 no.2
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    • pp.103-113
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    • 2018
  • Purpose: This study was performed to assess problems associated with sleep (short and long sleep duration) and to identify risky subgroups with sleep problems among adult cancer survivors. The study is based on the Korea National Health and Nutrition Examination Survey (KNHANES VI and VII) from 2013 to 2016. Methods: The sociodemographic and clinical data of 504 Korean cancer survivors aged 20-64 years was extracted from the KNHANES VI and VII database. Descriptive statistics for complex samples was used, and decision-tree analyses were performed using the SPSS WIN 24.0 program. Results: The mean age for survivors was approximately 51 years. The mean sleep duration was 6.97 hours; 36.2% of participants had short (< 7 hours) and 9.9% had long (> 8 hours) sleep duration. From the decision-trees analyses, the characteristics of the adult cancer survivors related to sleep problems were presented with six different pathways. Sleep problems were analyzed according to the survivors' sociodemographic information (age, education, living status, and occupation), clinical characteristics (body mass index, hypercholesterolemia, and anemia) and health-related quality of life (HRQoL). The HRQoL (${\leq}0.5$ or > 0.5 cutoff point) was a significant predictor of the participants' sleep problems because all six pathways were started from this predictor in the model. Conclusion: Health care professionals could use the decision-tree model for screening adult cancer survivors with sleep problems in clinical or community settings. Nursing interventions considering these specific individual characteristics and HRQoL level should be developed to have adequate sleep duration for Korean adult cancer survivors.

A Case of Respiratory Failure after Clean-up Work of the Hebei Spirit Crude Oil Spill in Taean (태안 Hebei Spirit 유출 유류 제거작업 후 발생한 호흡부전 1예)

  • Kyung, Sun Young;Chon, Su-Yeon;Kim, Yu Jin;Lee, Sang Pyo;Park, Jeong-Woong;Jeong, Sung Hwan
    • Tuberculosis and Respiratory Diseases
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    • v.67 no.3
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    • pp.249-253
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    • 2009
  • On 7 December 2007, the Hebei Spirit ran aground near Taean and released approximately 10,900 tons of oil into the sea. Crude oil on the coastal areas and fumes in the air increased the number of health problems among the local population. We report a case of respiratory failure after the oil spill clean-up work. A 66-year-old female was admitted to hospital with cough, sputum, and dyspnea of 1-month duration after the oil spill clean-up. She was diagnosed with community-acquired pneumonia and treated with empirical antibiotics. However, she had progressive respiratory failure without identification of the pathogen. Respiratory failure due to chronic inhalation of hydrocarbons from the crude oil spill clean-up was suspected. After mechanical ventilation care, she recovered from respiratory failure and was discharged. We report a case of severe respiratory toxic effects after an oil spill clean-up. We concluded that long-term hydrocarbon inhalation during the oil spill clean-up may have induced respiratory failure in this case.

The Effects of Alcohol Use, Depression, and Self-esteem on Suicidal Ideation of the Aged (노인의 알코올 사용, 우울 및 자존감이 자살생각에 미치는 영향)

  • Kwak, Yun-Bock;Ko, Sung-Hee;Kim, Hyun-Kyung
    • Journal of Digital Convergence
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    • v.11 no.9
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    • pp.221-228
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    • 2013
  • The purpose of this study was to examine the effects of alcohol use, depression, and self-esteem on suicidal ideation of older adults. Community-dwelling older adults aged 65 years and older were recruited from three welfare centers. A total of 245 older adults was assessed with the Alcohol Use Disorder Identification Test-Korean (AUDIT-K), Geriatric Depression Scale Short Form-Korea Version (GDSSF-K), Self-Esteem Scale (SES), and Scale for Suicidal Ideation (SSI). Descriptive statistics, Pearson's correlation, independent t-test, one-way ANOVA, and multiple regression analysis were performed using the SPSS WIN 19.0 program. Higher depression, lower self-esteem, no religion, and poorly perceived health were statistically significant determinants of higher level of suicidal ideation in older adults. To prevent or reduce suicidal ideation of older adults, health care providers should consider the determinants identified in this study.

Distribution and Properties of Microorganisms in Soil of Representative Vegetation of Mt. Nam (남산 주요 식생의 토양 미생물의 분포 및 생리적 특성)

  • 성치남;백근식;김종홍;전영문;김정근
    • The Korean Journal of Ecology
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    • v.21 no.5_3
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    • pp.703-712
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    • 1998
  • Physicochemical factors, microbial population size and the properties of the bacterial isolates were estimated to find out the nature of soil ecosystem of Mt. Nam. Samples were obtained from the surface layer of soils on which specific plant community is developed. Average content of moisture and organic matter of the soils were 21.6% and 17.3%, respectively. These values were similar to those of developing forest soils, but were slightly lower than those of climax ecosystem such as Piagol in Mt. Chiri. Chiri. Content of phosphate was higher than those of other forest soils. The population size of soil bacteria ranged from 27.4 to 195.8 ${\times}\;10^5$ CFU/g. duy soil, and the size is somewhat dependent on the moisture and oranic matter content of soils. A large number of bacteria were able to decompose macromolecules such as starch, elastin and gelatin. Bacterial species composition of each soil was comparatively simple. Pseudomonas, Agrobacterium, Flavobacterium and Xanthomonas which are Gram-negative short rods were widely distributed in the forest soils. The endospore forming Bacillus species were also the main constituents of the soil microflroa. Actinomycetes were widely distributed in the forest soils, but the distribution pattern varied in each site. Most of the actinomycetes were also able to decompose organic macromolecules. The rate of resistant actinomycete strains to antibiotics and heavy metals were lower than those from cultivated soils, but higher than those from well-preserved forest soils. Antibiosis pattern of the actinomycete isolates was similiar to the resistance pattern. This means the forest soils of Mt. nam was somewhat interferred by artificial behabiour.

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Removal of Organic Load from Olive Washing Water by an Aerated Submerged Biofilter and Profiling of the Bacterial Community Involved in the Process

  • Pozo, Clementina;Rodelas, Belen;Martinez-Toledo, M. Victoria;Vilchez, Ramiro;Gonzalez-Lopez, Jesus
    • Journal of Microbiology and Biotechnology
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    • v.17 no.5
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    • pp.784-791
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    • 2007
  • The present work aims to use a biofilter technology(aerated submerged filters) for the aerobic transformation at laboratory-scale of olive washing water(OWW) generated in the first steps of olive oil processing, as well as the genetic profiling and identification to the species level of the bacteria involved in the formation of the biofilm, by means of TGGE. Chemical parameters, such as biological oxygen demand at five days($BOD_5$) and chemical oxygen demand(COD), decreased markedly(up to 90 and 85%, respectively) by the biological treatment, and the efficiency of the process was significantly affected by aeration and inlet flow rates. The total polyphenol content of inlet OWW was only moderately reduced(around 50% decrease of the inlet content) after the biofilter treatment, under the conditions tested. Partial 16S rRNA genes were amplified using total DNA extracted from the biofilm and separated by TGGE. Sequences of isolated bands were mostly affiliated to the $\alpha-subclass$ of Proteobacteria, and often branched in the periphery of bacteria] genera commonly present in soil(Rhizobium, Reichenowia, Agrobacterium, and Sphingomonas). The data obtained by the experimentation at laboratory scale provided results that support the suitability of the submerged filter technology for the treatment of olive washing waters with the purpose of its reutilization.

Antibiotic Resistance Patterns of Staphylococcus aureus Isolated from the Specimen of University Students in Busan in 2004 (2004년 부산시내 일부 대학생의 검체에서 분리된 황색포도상구균의 항생제 내성 양상)

  • Kim, Tae-Un;Kim, Yun-Tae;Kwon, Heon-Young
    • Korean Journal of Clinical Laboratory Science
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    • v.37 no.3
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    • pp.155-163
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    • 2005
  • The purpose of this study is to investigate the carrier rate of S. aureus in the community, antibiotic susceptibility patterns of the organism, detection of MRSA and mecA gene in MRSA. Identification and antibiotic resistance patterns of S. aureus and MRSA were done by MicroScan Panels. MRSA strain was confirmed by disk diffusion method using oxacillin disk. The mecA gene in MRSA was detected by PCR. Eighty-four strains (27.4%) of S. aureus were isolated from the nasal specimens of 307 university students in Busan in 2004. Sixty-eight strains (81.9%) of 83 S. aureus were resistant to penicllin, 16 strains(19.3%) to erythromycin, 15 strains (18.1%) to gentamicin, 12 strains (14.5%) to tetracycline, 6 strains (7.2%) to chloramphenicol, 3 strains (3.6%) to ofloxacin, 2 strains (2.4%) to cefepime, clindamycin, imipenem, meropenem, norfloxacin, respectively. One strain (1.2%) was resistant to ciprofloxacin, cefazolin, cefotaxime, cefuroxime, and oxacillin. And all the strains (100%) of 84 S. aureus were susceptible to amoxicilin/K clavulanate, ticarcillin/K clavulanate, trimethoprim/sulfamethoxazole, rifampin, syncroid, teicoplanin, and vancomycin. One strain of 84 S. aureus isolates was methicillin-resistant Staphylococcus aureus (MRSA). The mecA gene was detected from the MRSA strain.

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Global Utopia and Local Anxiety on the Stage of the Korean Musical

  • Choi, Sung Hee
    • Cross-Cultural Studies
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    • v.36
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    • pp.123-147
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    • 2014
  • The purpose of this essay is three-fold: to trace the genealogy of the Korean musical, which ever since its inception in the 1960s has been seeking to modernize Korean theater with Broadway as a constant role model; to investigate how the national and the global conflict and are conflated in the form of the Korean musical in the process of its (dis)identification with Broadway; and to examine how its intercultural translations reveal and reflect the dilemma and ambivalence posed by globalization in our era. Drawing on Richard Dyer's signature article Entertainment and Utopia, I analyze how the Korean musical manifests and conduits competing utopian impulses of Korean/Global audiences. I also attempt to problematize the formulaic notion of Broadway musicalsthe Superior Other!which implies a global hegemony that does not, in fact, exist because the boundary between the global and the local as well as the power dynamics of global culture are not fixed but constantly moving and changing. Today's musical scene in Korea shows interesting reversals from the 1990s, when Korean producers were eager to debut on Broadway and impress American audiences. Korean producers no longer look up to Broadway as a final destination; instead they want to make Seoul a new Broadway. They import Broadway musicals and turn them into Korean shows. The glamor of Broadway is no longer the main attraction of musicals in Korea. What young audiences look for most is the glamor of K-pop idols and utopian feelings of abundance, energy, intensity, transparency and community, which they can experience live in the musical with their favorite stars right in front of their eyes. In conclusion, I delve into the complex dynamics of recent Korean musicals with Thomas Friedman's theory of Globalization 3.0 as reference. The binary formula of Global/America versus Local/Korea cannot be applied to the dynamic and intercultural musical scene of today. Globalization is not a uniform phenomenon but rather a twofold (multifold) process of global domination and dissemination, in which the global and the local conflict and are conflated constantly. As this study tries to illuminate, the Korean musical has evolved in a huge net of interdependences between the global and the local with a range of sources, powers and influences.

Management Effectiveness Evaluation(MEE) in Protected Areas for Forest Genetic Resources (산림유전자원보호구역의 관리효과성 평가 적용)

  • Ryu, Kwang-Su;Choi, Jae-Yong;Shin, Hyun-Tak
    • Journal of Forest and Environmental Science
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    • v.27 no.3
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    • pp.205-210
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    • 2011
  • This study aims to assess MEE(management effectiveness evaluations) on PAs(protected areas) for forest genetic resources which play an important role in biodiversity conservation, and then to suggest better ways to manage PAs for forest genetic resources. This study applies same indicators of the MEE on PAs as the ones described in the prior study(Ryu et al. 2011). The indicators applied are composed of five elements, thirty-two indicators which all would be grouped into one element by each traits. Overall indicators belonging to the element of output and outcome are comparatively low. Especially the ones related to the change of biodiversity, degree of ecosystem health, variation of civil complaint and visitor satisfaction are ranked mostly low. The element of input shows the low rank on the number of staff and budget. The score of indicators related to the identification of the threats of PAs and local communities' supports turn out to be low in the element of Context. The element of process, however, has scored low on Staff management, Education/Awareness programs and Governance, while the law enforcement, management regulation and capacity to prevent forest disasters have made relatively high score. Meanwhile, all indicators in the element of planning have scored relatively high as compared to the indicators belonged to other elements. This study suggests to strengthen a few constructive proposals, such as facilitating efficient management framework for PAs, developing local community cooperation program, establishing survey, research and monitoring system, and registering PAs to the WDPA(World Database on Protected Areas) according to the IUCN categories.

Identification of Knowledge Structure of Pain Management Nursing Research Applying Text Network Analysis (텍스트네트워크분석을 적용한 통증관리 간호연구의 지식구조)

  • Park, Chan Sook;Park, Eun-Jun
    • Journal of Korean Academy of Nursing
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    • v.49 no.5
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    • pp.538-549
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    • 2019
  • Purpose: This study aimed to explore and compare the knowledge structure of pain management nursing research, between Korea and other countries, applying a text network analysis. Methods: 321 Korean and 6,685 international study abstracts of pain management, published from 2004 to 2017, were collected. Keywords and meaningful morphemes from the abstracts were analyzed and refined, and their co-occurrence matrix was generated. Two networks of 140 and 424 keywords, respectively, of domestic and international studies were analyzed using NetMiner 4.3 software for degree centrality, closeness centrality, betweenness centrality, and eigenvector community analysis. Results: In both Korean and international studies, the most important, core-keywords were "pain," "patient," "pain management," "registered nurses," "care," "cancer," "need," "analgesia," "assessment," and "surgery." While some keywords like "education," "knowledge," and "patient-controlled analgesia" found to be important in Korean studies; "treatment," "hospice palliative care," and "children" were critical keywords in international studies. Three common sub-topic groups found in Korean and international studies were "pain and accompanying symptoms," "target groups of pain management," and "RNs' performance of pain management." It is only in recent years (2016~17), that keywords such as "performance," "attitude," "depression," and "sleep" have become more important in Korean studies than, while keywords such as "assessment," "intervention," "analgesia," and "chronic pain" have become important in international studies. Conclusion: It is suggested that Korean pain-management researchers should expand their concerns to children and adolescents, the elderly, patients with chronic pain, patients in diverse healthcare settings, and patients' use of opioid analgesia. Moreover, researchers need to approach pain-management with a quality of life perspective rather than a mere focus on individual symptoms.