• Child Health Nursing Research
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• v.27 no.3
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• pp.201-210
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• 2021

Purpose: This study analyzed research trends related to childhood and adolescent cancer survivors (CACS) using word co-occurrence network analysis on studies registered in the Korean Citation Index (KCI). Methods: This word co-occurrence network analysis study explored major research trends by constructing a network based on relationships between keywords (semantic morphemes) in the abstracts of published articles. Research articles published in the KCI over the past 10 years were collected using the Biblio Data Collector tool included in the NetMiner Program (version 4), using "cancer survivors", "adolescent", and "child" as the main search terms. After pre-processing, analyses were conducted on centrality (degree and eigenvector), cohesion (community), and topic modeling. Results: For centrality, the top 10 keywords included "treatment", "factor", "intervention", "group", "radiotherapy", "health", "risk", "measurement", "outcome", and "quality of life". In terms of cohesion and topic analysis, three categories were identified as the major research trends: "treatment and complications", "adaptation and support needs", and "management and quality of life". Conclusion: The keywords from the three main categories reflected interdisciplinary identification. Many studies on adaptation and support needs were identified in our analysis of nursing literature. Further research on managing and evaluating the quality of life among CACS must also be conducted.

• The Korean Journal of Mycology
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• v.46 no.4
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• pp.427-435
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• 2018

In this study, endophytic fungi were isolated from the surface-sterilized roots of Calanthe discolor and Cephalanthera longibracteata collected from the Chungnam, Jeju, Kyungnam and Chungbuk provinces in Korea. The morphological characteristics of the obtained isolates were examined and their sequences of the internal transcribed spacer (ITS) rDNA region were analyzed using the ITS1F and ITS4 primers for species identification. Leptodontidium orchidcola showed the highest species abundance and frequency among the isolated endophytic fungi. Additionally, the community analysis revealed a high specificity between the host plants and the endophytic fungal species.

• Journal of dental hygiene science
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• v.19 no.2
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• pp.86-95
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• 2019

The modern era of microbial genome analysis began in earnest in the 2000s with the generalization of metagenomics and gene sequencing techniques. Studying complex microbial community such as oral cavity and colon by a pure culture is considerably ineffective in terms of cost and time. Therefore, various techniques for genomic analysis have been developed to overcome the limitation of the culture method and to explore microbial communities existing in the natural environment at the gene level. Among these, DNA fingerprinting analysis and microarray chip have been used extensively; however, the most recent method of analysis is metagenomics. The study summarily examined the overview of metagenomics analysis techniques, as well as domestic and foreign studies on disease genomics and cluster analysis related to oral metagenome. The composition of oral bacteria also varies across different individuals, and it would become possible to analyze what change occurs in the human body depending on the activity of bacteria living in the oral cavity and what causality it has with diseases. Identification, isolation, metabolism, and presence of functional genes of microorganisms are being identified for correlation analysis based on oral microbial genome sequencing. For precise diagnosis and treatment of diseases based on microbiome, greater effort is needed for finding not only the causative microorganisms, but also indicators at gene level. Up to now, oral microbial studies have mostly involved metagenomics, but if metatranscriptomic, metaproteomic, and metabolomic approaches can be taken together for assessment of microbial genes and proteins that are expressed under specific conditions, then doing so can be more helpful for gaining comprehensive understanding.

• International Journal of High-Rise Buildings
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• v.7 no.4
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• pp.265-285
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• 2018

This paper presents a state-of-the-art review on the design, research and education aspects of fire safety engineering (FSE) with a particular concern on high-rise buildings. FSE finds its root after Great Fire of Rome in 64 AD, followed by Great London Fire in 1666. The development of modern FSE is continuously driven by industry revolution, insurance community and government regulations. Now FSE has become a unique engineering discipline and is moving towards performance-based design since 1990s. The performance-based fire safety design (PBFSD) involves identification of fire safety goals, design objectives, establishment of performance criteria, and selection of proper solutions for fire safety. The determination of fire scenarios and design fires have now become major contents for PBFSD. To experience a rapid and positive evolution in design and research consistent with other engineering disciplines, it is important for fire safety engineering as a profession to set up a special educational system to deliver the next-generation fire safety engineers. High-rise buildings have their unique fire safety issues such as rapid fire and smoke spread, extended evacuation time, longer fire duration, mixed occupancies, etc., bringing more difficulties in ensuring life safety and protection of property and environment. A list of recommendations is proposed to improve the fire safety of high-rise buildings. In addition, some source information for specific knowledge and information on FSE is provided in Appendix.

• Korean Journal of Clinical Laboratory Science
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• v.50 no.4
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• pp.375-381
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• 2018

Cytogenetic analysis plays an important role in examinations of a variety of human disorders. Over the years, cytogenetic analysis has evolved to a great extent and become a part of routine laboratory testing; the analysis provides significant diagnostic and prognostic results for human diseases. Microarray in conjunction with molecular cytogenetics and conventional chromosome analysis has transformed the outcomes of clinical cytogenetics. The advantages of microarray technologies have become obvious to the medical and laboratory community involved in genetic diagnosis, resulting in greatly improved visualization and validation capabilities. This article reviews how the field is moving away from conventional cytogenetics towards molecular approaches for the identification of pathogenic genomic imbalances and discusses practical considerations for the routine implementation of these technologies in genetic diagnosis.

• ALGAE
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• v.34 no.1
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• pp.57-70
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• 2019

DNA metabarcoding is currently used for large-scale taxonomic identification to understand the community composition in various marine ecosystems. However, before being widely used in this emerging field, this experimental and analytic approach still has several technical challenges to overcome, such as polymerase chain reaction (PCR) bias, and lack of well-established metabarcoding markers, a task which is difficult but not impossible to achieve. In this study, we present an adapted PCR-free small-organelles enriched metagenomics (SoEM) method for marine biodiversity assessment. To avoid PCR bias and random artefacts, we extracted target DNA sequences without PCR amplification from marine environmental samples enriched with small organelles including mitochondria and plastids because their genome sequences provide a valuable source of molecular markers for phylogenetic analysis. To experimentally enrich small organelles, we performed subcellular fractionation using modified differential centrifugation for marine environmental DNA samples. To validate our SoEM method, two marine environmental samples from the coastal waters were tested the taxonomic capturing capacity against that of traditional DNA metabarcoding method. Results showed that, regardless of taxonomic levels, at least 3-fold greater numbers of taxa were identified in our SoEM method, compared to those identified by the conventional multi-locus DNA metabarcoding method. The SoEM method is thus effective and accurate for identifying taxonomic diversity and presents a useful alternative approach for evaluating biodiversity in the marine environment.

• Journal of English Language & Literature
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• v.64 no.2
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• pp.219-238
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• 2018

This essay examines the first-generation Korean American writer, No-Yong Park's falsehoods about his ethnic identity to suggest how and why he passed for Chinese, and to explore the political, anti-Japanese implications of these actions. The essay first identifies erroneous information circulating about his biographical background, presents some other materials that help us better understand the context in which he forged his Chinese identity, and then examines how he represented himself as Chinese in his published works. I would argue that Park's self-identification as Chinese was a resulting outcome of his naturalization caused by the Japanese colonial power in Korea and also one of his surviving strategies in the racist environment within American society. Looking at some of his works-including Making a New China (1929), An Oriental View of American Civilization (1934), Chinaman's Chance: Autobiography (1940)-and examining how he represented Korea and its people reveal how he tried to raise voice for them. By doing so, this essay illuminates Park's resistance to Japan's colonial discourse and power in Korea while revealing his lifetime passing as Chinese-far from his refusal to belong to the Korean community, or to acknowledge being Korean.

• Journal of Korean Dental Science
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• v.16 no.1
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• pp.35-46
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• 2023

Purpose: Culture-based methods for microbiological diagnosis and antibiotic susceptibility tests have limitations in the management of orofacial infections. We aimed to profile pus microbiota and identify antibiotic resistance genes (ARGs) using a culture-independent approach. Materials and Methods: Genomic DNA samples extracted from the pus specimens of two patients with orofacial abscesses were subjected to shotgun sequencing on the NovaSeq system. Taxonomic profiling and prediction of ARGs were performed directly from the metagenomic raw reads. Result: Taxonomic profiling revealed obligate anaerobic polymicrobial communities associated with infections of odontogenic origins: the microbial community of Patient 1 consisted of one predominant species (Prevotella oris 74.6%) with 27 minor species, while the sample from Patient 2 contained 3 abundant species (Porphyromonas endodontalis 33.0%; P. oris 31.6%; and Prevotella koreensis 13.4%) with five minor species. A total of 150 and 136 putative ARGs were predicted in the metagenome of each pus sample. The coverage of most predicted ARGs was less than 10%, and only the CfxA2 gene identified in Patient 1 was covered 100%. ARG analysis of the seven assembled genome/metagenome datasets of P. oris revealed that strain C735 carried the CfxA2 gene. Conclusion: A metagenomics-based approach is useful to profile predominantly anaerobic polymicrobial communities but needs further verification for reliable ARG detection.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.31 no.1
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• pp.43-56
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• 2023

The aviation industry has continued to grow despite the influence of external environmental factors such as SARS and MERS. However, this growth has led to a sharp decrease in air traffic and a crisis of flight suspension due to the collapse of the global value chain and the decline of the world economy as the World Health Organization (WHO) declared a COVID-19 pandemic. Due to the decrease in the number of international flights, aviation workers such as pilots and cabin crew were exposed to high psychological stress and fatigue, such as large-scale layoffs, job instability, decrease in income, and increased risk of infection during. Recently, the international community has eased immigration restrictions through quarantine activities, and airlines are taking a step further to recover existing air demand. However, during the crisis period, a significant number of professional workers have been turned off or fired, and the physical and mental fatigue of those who can perform their duties is increasing. Therefore, this paper intends to examine the direction of policy improvement and the identification of problems in aviation safety and fatigue management after the pandemic.

• Korean Journal of Agricultural Science
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• v.50 no.4
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• pp.799-808
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• 2023

Globally, abnormal climate phenomena have led to an increase in rainfall intensity, consequently causing a rise in flooding-related damages. Agricultural areas, in particular, experience significant annual losses every year due to a lack of research on flooding in these regions. This study presents a comprehensive analysis of the flood event that occurred on July 16, 2017, in the agricultural area situated in Sindaedong, Heungdeok-gu, Cheongju-si. To achieve this, the EPA (United States Environmental Protection Agency) Storm Water Management Model (SWMM) was employed to generate runoff data by rainfall information. The produced runoff data facilitated the identification of flood occurrence points, and the analysis results exhibited a strong correlation with inundation trace maps provided by the Ministry of the Interior and Safety (MOIS). The detailed output of the SWMM model enabled the extraction of time-specific runoff information at each inundation point, allowing for a detailed understanding of the inundation status in the agricultural area over different time frames. This research underscores the significance of utilizing the SWMM model to simulate inundation in agricultural areas, thereby validating the efficacy of flood alerts and risk management plans. In particular, the integration of rainfall data and the SWMM model in flood prediction methodologies is expected to enhance the formulation of preventative measures and response strategies against flood damages in agricultural areas.