• Journal of Mushroom
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• v.6 no.3_4
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• pp.121-125
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• 2008

A first, we made white strain 'S line' adaptable to elevated temperature after collected brown strains were characterized with culture characterization on temperature and their monokaryons isolated at the high temperature were crossed with white strain. These S line monokaryons were crossed again with other monokaryons derived from a commercial white. As a result of successive crossing, finally a new variety 'Baengno' was developed. The optimum temperature of mycelial growth was $25^{\circ}C$ but it needed to adjust to $18{\sim}20^{\circ}C$ when incubated at the bottle cultivation. It brought incubation period shortening as 21 days saved 2~3 days when compared with other white varieties. The optimum temperatures of fruiting body initiation and development were almost same to others like as $14^{\circ}C$ and $7^{\circ}C$, respectively. Fruiting body of 'Baengno' was pure white even developed from crossing with brown strains. 'Baengno' was a good variety with high quality and high productivity characterized as quite even budding habit, long stipes and hemi-spherical pilei. This variety needed more air ventilation and had to be adjusted its cultivation environment for the good cultivation.

• The Korean Journal of Mycology
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• v.34 no.1
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• pp.29-33
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• 2006

To develop environmentally friendly bio-materials for animal feed, the potential of using mushroom was studied. Adding Flammulina velutipes cultivation media wastes (FCMW) has shown beneficial effects on broiler meat quality and production cost because of the $10{\sim}20%$ saving in feeds for broiler chicken. However, differences proximate composition between FCMW and commercial broiler chicken feed were observed. FCMW had higher contents of crude fat (6.2%) and crude fiber (13.7%) than the commercial broiler feed which had crude fat and crude fiber of 4.0%, and 6.0%, respectively. But FCMW crude protein contents were lower (12.2%) than those in commercial broiler (19.0%). Adding $5{\sim}10%$ of FCMW to broiler chicken feed increased amino acid concentration upto $33{\sim}38%$ in the meat, but adding more than 20% decreased amino acid concentration.

• 한국균학회소식:학술대회논문집
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• 2014.05a
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• pp.45-45
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• 2014

Individualities of precious health mushroom called Zhenghonggu@ from respective protections scattered among all main mountains of Fujian China were collected and recognized locally, then compared with Russula griseocarnosa. Their internal transcribed spacer (ITS) region (ITS1, ITS2 and 5.8S rDNA) of the nuclear rDNA were amplified, AMOVA analyzed, nested clade analyzed and then compared with the ITS sequences of relative Russula species from other regions of China to confirm the taxonomic status of Zhenghonggu$^@$ and its population structure. Total 23 haplotypes from different protections of Fujian can be clustered into three clades similar to the three lineages of Dahongjun$^@$ from southeastern China reported by Li et al. The geographic distribution characteristic of these three phylogeny clades may be closely coupled with the vegetation regionalization and/or the differences of coenosium construction of Fagaceae that is the host of Russula griseocarnosa. The correlation of taxonomy, phylogeny and geographical distribution of Russula are discussed.

• Journal of Mushroom
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• v.12 no.4
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• pp.363-366
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• 2014

This study was focused on finding a relationship between fruit-body growth and relative humidity in Neolentinus lepideus cultivation. The higher the relative humidity, the moisture content of pileus and stipe were also tends to be increased. The day of formation of primordia and development of fruit body was shortest at the relative humidity 95%. The yield and commercial yield were higher in the relative humidity 95% than in the other treatment. Individual weight of fruit body was the highest at the relative humidity 75% and 85%. Crack indices of pileus was the strongest at the relative humidity 65%.

• Journal of Mushroom
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• v.8 no.4
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• pp.161-164
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• 2010

For the development of a sporeless strain of P. ostreatus we used sporeless strain ASI 2069. We have recovered both nuclear types of strain ASI 2069 as monokaryons of nh9, nh15, nh26 and nh36 (here after referred to as neohaplonts) by protoplasting the mycelium. Crosses between neohaplonts and SSI's(single spore isolates) obtained from a sporulating commercial strain ASI 2180. Five excellent strains are selected from 30 bred strains by quality of fruitbodies and spore number. To development of molecular markers linked to sporeless strain of P. ostreatus, we are screened helicase, recombinase(DMC1) and topoisomerase(Spo11) genes related meiosis by PCR and sequencing. Among three genes, Spo11 gene was identified into molecular marker of sporeless from neohaplonts and bred strains of P. ostreatus.

• Journal of Mushroom
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• v.12 no.4
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• pp.270-274
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• 2014

In previous studies, we selected the green LED(light emitting diodes) for suitable wavelength of the light by higher commercial yields and lower ratio of the abnormal fruit body in Lentinula edodes. In this study, we aimed to select efficient irradiation intensity of the green LED. Stronger irradiation intensity of the green LED resulted in more polyphenol content of fruit body. And Polyphenol content of fluorescent lamp was similar to that of the green LED $20umol{\cdot}m^{-2}{\cdot}s^{-1}$.. Ergosterol content of the green LED $5umol{\cdot}m^{-2}{\cdot}s^{-1}$ was showed higher 2.1 times than that of the fluorescent lamp. In four level of irradiation intensity, 5, 10, 15, $20umol{\cdot}m^{-2}{\cdot}s^{-1}$ there was no big difference in characteristics of the fruit body. However the yield of fruit body in the green LED $5umol{\cdot}m^{-2}{\cdot}s^{-1}$ is higher than the others. In addition, The amount of electricity used of the green LED $5umol{\cdot}m^{-2}{\cdot}s^{-1}$ was reduced 15.9% than that of the fluorescent lamp. In conclusion, we selected $5umol{\cdot}m^{-2}{\cdot}s^{-1}$ for suitable irradiation intensity in Lentinula edodes with sawdust substrate cultivation.

• Journal of The Korean Society of Grassland and Forage Science
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• v.34 no.1
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• pp.45-51
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• 2014

This study was conducted to investigate the feeding effects of total mixed fermentations (TMF) with spent mushroom (Flammuliua velutipes) and wet brewer's grain on estimating the productivity and nutrient utilization of Korean black goats. Four experimental groups were set up with each of the 5 animals for a 30-day feeding trial on growth performance. 12 black goats with regard to nutrient digestibility were allocated to 4 groups of 3 animals each by a latin-square design control group (offered concentrate and rice straw) and 3 treatment groups (TMF with 15% spent mushroom and 25% wet brewer's grain (T1), 10, 30% (T2) and 5, 35% (T3)). The average daily gain (ADG) of the control and T1 treatment groups was higher than that of the T3 treatment group (p<0.05). Dry matter (DM) and organic matter (OM) intake (kg/head) for T1 and T2 treatment groups were significantly higher than those of the control group (p<0.05). The digestibility of DM and OM for the control group (62.39~63.39%) and the T1 treatment group (65.02~65.25%) were significantly higher than those of the T3 treatment group (56.39, 59.01%) (p<0.05). Further, nitrogen retention was significantly higher in the T1 treatment group (p<0.05). Thus, these results conveyed that productivity and nutrient utilization were similar between TMF with spent mushroom and wet brewer's grain and commercial feeds. However, this study also suggested that feed must contain 25~30% spent mushroom due to the fact that the productivity of Korean black goats was decreased by increasing the content of spent mushroom.

• Bulletin of the Korean Chemical Society
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• v.28 no.5
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• pp.758-762
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• 2007

A new alkyldithiocarbonate (xanthate), as sodium salts, C2H5OCS2Na, was synthesized by the reaction between CS2 with ethyl alcohol in the presence of NaOH. The new xanthate was characterized by 1H NMR, IR and elemental analysis. Then, the new synthesized compound was examined for functional study of cresolase activity of Mushroom Tyrosinase (MT) from a commercial source of Agricus bisporus in 10 mM phosphate buffer pH 6.8, at three temperatures of 10, 20 and 33℃ using UV spectrophotemetry. 4-[(4-methylphenyl)- azo]-phenol (MePAPh) was used as a synthetic substrate for the enzyme for cresolase reaction. The results show that ethyl xanthate can activate or inhibit the cresolase activity of mushroom tyrosinase depending to the concentration of ethyl xanthate. It was concluded that the enzyme has two distinct sites for ethyl xanthate. The first one is a high-affinity activation site and the other is a low-affinity inhibition site. Activation of the enzyme in the low concentration of ethyl xanthate arises from increasing the affinity of binding for the substrate as well as increasing the enzyme catalytic constant. The affinity of ligand binding in the activation site is decreased by increasing of the temperature, which is the opposite result for the inhibition site. Hence, the nature of the interaction of ethyl xanthate is different in two distinct sites. The binding process for cresolase inhibition is only entropy driven, meanwhile the binding process for cresolase activation is not only entropy driven but also enthalpy driven means that hydrophobic interaction is more important in the inhibition site.

• Journal of Practical Agriculture & Fisheries Research
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• v.24 no.3
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• pp.5-14
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• 2022

Trichoderma is known as pathogen caused serious green mold disease on commercial production. T. pleuroti and T. pleuroticola were common species in various mushroom media. Many strains of T. pleuroti, known as aggressive species causing major economic losses in Korea, showed benomyl resistance. Accurate identification and detection of Trichoderma species associated with oyster mushrooms is very important for disease control. We developed species-specific primers for T. pleuroticola, T. pleuroti, T. harzianum, and T. atroviride based on species-specific fragments isolated from amplified fragment length polymorphism analysis. PCR products corresponding to the predicted fragment of 500bp, 230bp, 180bp, and 410bp were amplified from T. pleuroticola, T. pleuroti, T. harzianum, and T. atroviride, respectively. Multiplex PCR assay using species-specific primers quickly and accurately identified and detected T. pleuroti from mushroom media in which various species co-exist. Our results can be useful for the effective control of mushroom disease.

• Journal of Mushroom
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• v.5 no.1
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• pp.1-6
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• 2007

A new commercial strain "Hambak" of Grifola frondosa was developed by di-mono crossing between dikaryon of ASI 9031 and monokaryotic strain derived from ASI 9021. It can be cultivated in plastic bottle and bag filled with oak and poplar sawdust substrate which supplemented with 15% rice bran or 10% corn bran. Improvement of cultivation methods was required for shortage of mycelium incubation period and management of humidity in growing room. The optimum temperature of mycelial growth was $25^{\circ}C$ and that of fruiting body development was $15{\sim}18^{\circ}C$. The color of pileus surface was brown and fine pores, whitish to cream-colored, were underside. Primordia formation of Hambak was two days faster than that of Yipsae 1. The yield was $97g/850m{\ell}$ bottle, a bunch of fruiting body was bigger and the quality was better than Yipsae 1.