A total of 120 pigs were used to investigate the effect of feeding probiotics on physico-chemical properties and sensory evaluation of pork loin. About 50kg pigs were randomly alloted into one of six experimental diet groups (C1:commercial diet feed the gilt; C2:commercial diet feed the barrow; T1:$0.5\%$ YC2000 feed the gilt T2:$0.5\%$ YC2000 feed the barrow; T3:$0.1\%$ YC2000 + $0.3\%$ KBC1121 feed the gilt; T4:$0.1\%$ YC2000 + $0.3\%$ KBC1121 feed the barrow). Pigs were slaughtered at approximately 110kg live weight. Crude fat and crude ash were not difference among the treatments. However, water content was higher in T1 and T2 compared to other treatment and the protein level of T3 was higher than those of other treatments. All of dietary probiotic groups showed higher pH compared to control. Especially, pH of T1 and T2 were higher among the dietary probiotic groups. Cholesterol level of dietary probiotic groups were lower compared to control. In meat color, $a^{*}$ was higher in T1 and $b^{*}$ was lower in T2 compared to other treatments. In sensory evaluation of cooked meat, aroma, flavor, tenderness, juiciness and overall palatability were higher in control, whereas T3 and T4 showed higher score in tenderness, juiciness and overall palatability. T3 had higher myristic acid. palmitoleic acid and oleic acid, whereas arachidonic acid was lower in T3. In conclusion, dietary probiotic groups were much better than other treatments in cholesterol, color, tenderness and juiciness. But drip loss of dietary probiotic groups showed higher due to lower pH compared to control.
Kim Mu-Chan;Kang Chang-Keun;Park Hye-Young;Lee Dae-Seong;Kim Yun-Sook;Lee Won-Jae
Korean Journal of Microbiology
/
v.42
no.2
/
pp.111-115
/
2006
A feeding trial was conducted to test the use of marine yeasts isolated from seawaters and sediments as a dietary source in cultivating a Cladocera, Moina macrocopa which is available as an alternative live food for fish larvae. The marine yeast-fed M. macrocopa had similar essential amino acid profiles to the documented values for Rotifers and Artemia enriched in microalgae and commercial diets. Erythrobacter sp. $S{\pi}-1$ lacked ${\omega}-3$ high unsaturated fatty acids (HUFAs), $20:5{\omega}-3$ (EPA) and $22:6{\omega}-3$ (DHA), which were also poor but detected in both the marine yeasts. An increase in the $20:5{\omega}-3$ and $22:6{\omega}-3$ levels, compared with the levels in marine yeast strains themselves, was more pronounced in the $22:6{\omega}-3$ level of Moina fed the Candida sp. Y-16, resulting in a high DHA:EPA ratio. When the Moina diets were switched, their ${\delta}^{13}C$ values shifted gradually toward the values of the switched diets. Diet switch from Erythrobacter sp. $S{\pi}-1$to Candide sp. Y.16 resulted in a more rapid turnover of Moina tissue carbon than that in the inverse case. When fed a mixed diet, the ${\delta}^{13}C$ values of Moina tissue approached the value of marine yeasts immediately. These temporal changes in the ${\delta}^{13}C$ values of Moina tissue indicate the preferential ingestion of marine yeasts and a selective assimilation of the carbon originated from marine yeasts. These findings suggest that marine yeasts, particularly Candida sp. Y-16, are highly available to mass cultures of M. macrocopa, providing better nutritional and dietaty values than the commercial diet (Erythrobacter sp. $S{\pi}-1$).
Two experiments were conducted to compare the effects of the natural and synthetic commercial pigments on the laying Performance, Pigmentation and fatty acid contents in e99 yolk of laying hens. The experimental diets were formulated to have isocalories and isonitrogen. In experiment I, the diet does not contain the com in which the xanthophylls are free, and in experiment II, the diet contained 54% of corn in which the level of xanthophylls are 19.34 ppm/kg (calculated levels). In the experiment 1, 480 ISA Brown laying hens were divided into eight groups. Each group has 60 birds fed the 0% of corn with 8 types of pigment levels for 4 weeks. in the experiment 2, 600 ISA Brown laying hens were divided into ten groups. Each group has 60 birds fed the 54% of com diet with 10 types of pigment levels for 4 weeks. Feed intake, e99 Production, egg weight and feed efficiency did not have significant difference in experiments I and II. Albumen height and haugh unit did not have significant difference in both experiments. In order to approach the yolk pigmentation to 12∼13 of Roche color fan, addition level of natural red pigment was 25∼30 ppm. In the case of synthetic red pigment, the level was 15∼20 ppm. In this experimental condition, the pigmenting effect of the synthetic pigment was better than that of the natural pigment. In the experiment 2, the pigmenting effect of mixing pigments were investigated between TM2 mixed with natural red pigment, and TM6 mixed with synthesis red pigment. The pigmenting effect of synthetic red pigment was greater than that of the natural red pigment. However, the pigmenting effect of natural pigment was greater than that of synthetic pigments when the levels of synthetic and natural red pigments in diet are higher in TM3 and TM8. The fatty acid content in yolk was not affected by pigment addition.
Kim, Ji-Hyuk;Kang, Hwan-Gu;Bang, Han-Tae;Hwangbo, Jong;Choi, Hee-Cheol;Kim, Dong-Wook;Hong, Eui-Chul
Korean Journal of Poultry Science
/
v.42
no.1
/
pp.69-75
/
2015
This work was carried out to evaluate the feces and ileal digestibility of canola meal and wheat bran in ducks. Ninety Pekin ducks (6-week-old, average 3.4 kg) were divided into three treatments (6 replications/treatment, 5 birds/replication) by feedstuffs (basal diet, canola meal, and wheat bran) and were fed on these diets in individual metabolic cages for one week. After one week, the samples for analysis were collected from feces and ileum. On comparison of treatments and collected sites, the feces digestibility of basal diet was the highest among treatments on chemical compositions except crude protein and crude fat and ileal digestibility of canola meal was the lowest (P<0.01). On comparison of treatments, the digestibility of basal diet was higher and that of wheat bran was lower when compared to other treatments (P<0.05). The feces digestibility was lower than the ileal digestibility in crude protein (P<0.05) and crude fat (P<0.01). The digestibility of amino acid was the highest at feces of control group among treatments (P<0.01). No significant difference was detected in terms of digestibility of isoleucine, leucine and phenylalanine among essential amino acids, and alanine, glycine and tyrosine among non-essential amino acids between control and wheat bran, and wheat bran and canola meal. However amino acid digestibility was higher in control group when compared to canola meal group (P<0.05). Other amino acid digestibilities were not significantly differ by treatments. These results may provide the basic data on amino acid digestibility of ducks to evaluate feedstuffs and to formulate optimum diet for commercial Pekin duck.
KANG Young Jin;LEE Jong Yun;LEE Sang Min;HUR Sung Bum
Journal of Aquaculture
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v.5
no.2
/
pp.127-142
/
1992
A feeding experiment was carried out to investigate the availability and feasibility of fish processing by-products as a substitute for frozen round fish in moist pellet diets for flounder, Paralichthys olivaceus. A control diet was prepared by incorporating $50{\%}$ frozen horse mackerel and $50{\%}$ commercial binder feed. In test diets the frozen horse mackerel of the control diet was replaced with either tuna scrap, tuna viscera, flatfish offal, deskinned hagfish, or tuna viscera ($20{\%}$) with frozen horse mackerel ($30{\%}$). Eighty flounders, 13.4 g in mean body weight, were stocked in 12 aquaria ($250\;{\ell}$ each) and fed six experimental diets for the first 28-day experiment. For the second 23-day experiment, forty flounders,44.8 g in mean body weight, were stocked and fed the same experimental diets. All treatments were duplicated. There were no significant differences in growth, feed efficiency and nutrient utilization among fish fed experimental diets containing either frozen horse mackerel, deskinned hagfigh, flatfish offal or tuna viscera with frozen horse mackerel. However, the feeding performances of fish fed experimental diets containing tuna scrap or tuna viscera were significantly lower than those of the control diet (P<0.05). There were no significant differences among the treatments on the proximate analysis values of the whole body. However, the crude lipid content of liver of the fish fed the experimental diet containing tuna scrap was significantly lower than that of the others. The results indicate that deskinned hagfish, and flatfish offal could be successfully substituted for frozen fish portion of moist pellet diets for flounder, but tuna viscera could only be partially substituted.
This study was conducted to investigate the effects of dietary activated coconut charcoal (ACC) on Performance, e99 quality and Plasma cholesterol level of Plasma in laying hens. One hundred forty four, 47-wk-old, ISA Brown commercial layers were used in a 28-d feeding trial after a 7-d adjustment Period. Four dietary treatments were 0, 0.5, 1.0 and 1.5e% levees of ACC supplemented to a corn-soybean meal basal diet. Egg Production was significantly increased as the levels of ACC increased. However, egg weight was significantly decreased by the addition of ACC in diets. Egg shell breaking strength tended to decrease as the level of ACC increased, however, no significance was found in this respect. As the levels of ACC increased, egg shell thickness decreased significantly. Although not significant, yolk color tended to increase by the addition of ACC. Egg folk index were significantly increased by the addition of ACC In diet. No significant difference was found among four treatments in total cholesterol, HDL cholesterol, and LDL+VLDL cholesterol concentrations in plasma. In conclusion, dietary supplementation of ACC to layer diets could be used to increase egg Production, and yolk index.
The objective of this study was to evaluate the potential of an alumninosilicate clay, marketed under the trade name Biotite V, to improve growing-finishing pig performance and to determine its effects on nutrient digestibility and excretion. Sixty crossbred pigs (22.3${\pm}2.7kg$, Camborough 15 Line female${\times}$Canabred sire) were assigned on the basis of sex, weight and litter to one of four dietary treatments in a $2{\times}4$ (two sexes and four treatments) factorial design experiment. The experimental diets were based on barley and soybean meal and contained 0, 0.25, 0.5 or 0.75% biotite during the growing period (22.3-60.5 kg) and 0, 0.5, 1.0 or 1.5% biotite during the finishing period (60.5-110.3 kg). Each pig was allowed access to its own individual feeder for 30 min twice daily (07:00 and 15:00 h). Individual pig body weight, feed consumption and feed conversion were recorded weekly. The pigs were slaughtered at a commercial abattoir when they reached an average weight of 110.3 kg. Carcass weight was recorded and dressing percentage calculated. Carcass fat and lean measurements were obtained with a Destron PG 100 probe between the 3rd and 4th last ribs, 70 mm of the midline. Total tract digestibility coefficients for dry matter, energy, nitrogen and phosphorus were determined using three males and three females per treatment starting at an average weight of $52.2{\pm}3.8kg$. These pigs were housed under identical conditions as those used in the growing stage and were fed the same diets modified only by the addition of 0.5% chromic oxide as a digestibility marker. Over the entire experimental period (22.3-110.3 kg), daily gain was unaffected (p>0.05) by the inclusion of biotite in the diet. There was a cubic response for feed intake (p=0.06) and a quadratic response (p=0.07) for feed conversion due to biotite. Feeding biotite produced no significant (p>0.05) linear or quadratic effects on any of the carcass traits measured. Dry matter digestibility decreased linearly (p=0.02) with increasing levels of biotite in the diet. However, digestibility coefficients for energy, nitrogen and phosphorus were unaffected (p<0.05) by biotite inclusion. Lactobacilli and enterobacteria numbers were unaffected by inclusion of biotite while Salmonella was not detected in any of the fecal samples. The overall results of this experiment indicate that biotite inclusion did not reduce fecal excretion of nitrogen or phosphorus and failed to improve nutrient digestibility. Neither growth rate nor carcass quality was improved while a modest improvement in feed conversion was observed at lower levels of inclusion. Based on the results of this experiment, it would be difficult to justify the routine inclusion of biotite in diets fed to grower-finisher pigs. Whether or not a greater response would have been obtained with pigs of a lower health status is unknown.
Maize-soybean meal diets with 0, 100, 200 and 300 g/kg double zero rapeseed meal ('00' RSM) with and without an enzyme mixture (xylanase, pectinase, cellulase) at a level of 1.6 g/kg were evaluated with 624 day-old broiler chicks for 5 weeks. The birds were randomly allocated to eight dietary treatments with three replicates of 26 birds each. Average daily gain (ADG) and feed intake (FI) were recorded weekly and ileal viscosity, organ weights, serum enzyme activity, hormonal profile and hematological parameters were measured at the end of week 5. Average daily gain during the weekly periods was significantly influenced by the dietary level of '00'RSM (p<0.01). Inclusion of '00' RSM improved the ADG up to day 28 with the increased level; beyond that time no improvement was recorded when compared to control groups. However, ADG from 1-35 days was significantly different between 300 g/kg inclusion level of '00' RSM and the control diet. Inconsistent decline in feed intake and feed conversion ratio was observed up to day 21 and the trend was reversed thereafter. The proportion of '00' RSM in the diet had a significant ($p{\leq}0.05$) influence on thyroid weight but had no effect on the relative weights of liver and heart, serum enzyme activities (${\gamma}$-glutamyl transferase, alanine amino transferase and aspartate amino transferase), thyroid hormones ($T_3$ and $T_4$), hemoglobin level and hematocrit. Significant improvement in ADG was recorded during the 2nd week of age with the addition of enzyme, whereas for all other periods, including the whole period of the trial, higher but non-significant ADG was observed. FI and FCR were not affected by the addition of enzyme but there was a numerical reduction in FCR during the whole period. The addition of enzyme reduced the ileal viscosity at all levels of '00' RSM inclusion. The results suggest that '00' RSM can be included up to 300 g/kg in broiler diets without any adverse effects on health and performance. The addition of commercial enzyme mixture containing xylanase, pectinase, cellulase to broiler diets containing '00'RSM has some effect on growth rate and feed conversion efficiency.
Santos, Rozilda da Conceicao dos;Gomes, Daiany Iris;Alves, Kaliandra Souza;Mezzomo, Rafael;Oliveira, Luis Rennan Sampaio;Cutrim, Darley Oliveira;Sacramento, Samara Bianca Moraes;Lima, Elizanne de Moura;Carvalho, Francisco Fernando Ramos de
Asian-Australasian Journal of Animal Sciences
/
v.30
no.6
/
pp.865-871
/
2017
Objective: The aim was to evaluate carcass characteristics, cut yield, and meat quality in lambs that were fed different inclusion levels of palm kernel cake. Methods: Forty-five woolless castrated male Santa $In{\hat{e}}s$ crossbred sheep with an initial average body weight of $23.16{\pm}0.35kg$ were used. The experimental design was a completely randomized design with five treatments, with palm kernel cake in the proportions of 0.0%, 7.5%, 15.0%, 22.5%, and 30.0% with nine replications per treatment. After slaughter, the gastrointestinal tract was weighed when it was full, after which it was then emptied. The heart, liver, kidney, pancreas perirenal fat were also collected and weighed. The carcass was split into two identical longitudinal halves and weighed to determine the quantitative and qualitative characteristics. Results: The empty body weight, carcass weight and yield, and fat thickness decreased linearly (p<0.05) as a function of palm kernel inclusion in the diet. There was no difference (p>0.05) for the rib eye area of animals that were fed palm kernel cake. There was a reduction in the commercial cut weight (p<0.05), except for the neck weight. The weights of the heart, liver, kidney fat, small, and large intestine, and gastrointestinal tract decreased. Nevertheless, the gastrointestinal content was greater for animals that were fed increasing levels of cake. For the other organs and viscera, differences were not verified (p>0.05). The sarcomere length decreased linearly (p<0.05), although an effect of the inclusion of palm kernel cake was not observed in other meat quality variables. It is worth noting that the red staining intensity, indicated as A, had a tendency to decrease (p = 0.050). Conclusion: The inclusion of palm kernel cake up to 30% in the diet does not lead to changes in meat quality characteristics, except for sarcomere length. Nevertheless, carcass quantitative characteristics decrease with the use of palm kernel cake.
This study was carried out to investigate dietary effects of illite on humoral immune response against Salmonella typhimurium flagella in Hyline Brown laying hens. Total of twenty eight 36-week-old hens were divided into two groups; The first was fed commercial diet as control and the other was fed diet containing 2 % illite. Each group was divided into 2 sub-groups and then performed immunization of Salmonella flagella with different adjuvants which were Freund's adjuvant and croton oil. The rates of egg-production in all groups were normal range but no difference was found between illite-treated and untreated groups. The serum antibody titers of Freund's adjuvant-used subgroup in illite fed groups were significantly increased, especially from 6-to 9-week after $1^{st}$ immunization compared to those of control groups (p<0.05). And the antibody titers of croton oil-used subgroup in illite-fed groups were also significantly increased, especially at 4-, 6- and 7-week (p<0.05). The results demonstrated that the feeding illite stimulated the immune response against S. typhimurium flagella antigen in laying hens and suggest that the supplementation of illite to the poultry diets may support protective effects against bacterial infections such as Salmonellosis.
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