• 제목/요약/키워드: Colorimetric measurement

검색결과 49건 처리시간 0.024초

흡광광도법을 이용한 포름알데히드 확산측정기의 평가 (Evaluation of a Diffusive Sampler for the Measurement of Formaldehyde using Colorimetric Method)

  • 임봉빈;김선규;정의석;김선태
    • 대한환경공학회지
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    • 제27권6호
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    • pp.606-613
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    • 2005
  • 본 연구에서는 실내공기 중 포름알데히드를 측정하기 위해 세 가지 다른 반응의 흡광광도법(비색분석)을 응용한 뱃지형 확산측정기를 평가하였다. 흡수여지의 세척방법을 비교한 결과 사용된 세척용액의 종류에 관계없이 세척하지 않은 경우 보다 양호한 세척효과가 나타났다. 각 측정방법에서 사용될 흡수액의 농도는 시료채취율을 기준으로 정하였다. 평가된 공시험 값은 측정방법별로 15% 이내의 편차를 가진 것으로 나타나 대체적으로 안정적인 것으로 나타났다. 확산측정기의 재현성은 MBTH법이 상대표준편차 10% 이내로 가장 양호하며, 채취된 포름알데히드 양과 시간가중평균농도와의 상관관계는 세 가지 측정방법 모두 양호한 것으로 나타났다. 검출한계 및 정량한계는 시료채취율이 가장 좋은 MBTH법이 다른 측정방법과 비교하여 가장 낮게 나타나 시료채취율이 가장 크고 정량한계가 낮은 측정방법일수록 포름알데히드 측정에 요구되는 측정시간이 감소되는 것으로 나타났다.

Smartphone-based Chemistry Instrumentation: Digitization of Colorimetric Measurements

  • Chang, Byoung-Yong
    • Bulletin of the Korean Chemical Society
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    • 제33권2호
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    • pp.549-552
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    • 2012
  • This report presents a mobile instrumentation platform based on a smartphone using its built-in functions for colorimetric diagnosis. The color change as a result of detection is taken as a picture through a CCD camera built in the smartphone, and is evaluated in the form of the hue value to give the well-defined relationship between the color and the concentration. To prove the concept in the present work, proton concentration measurements were conducted on pH paper coupled with a smartphone for demonstration. This report is believed to show the possibility of adapting a smartphone to a mobile analytical transducer, and more applications for bioanalysis are expected to be developed using other built-in functions of the smartphone.

비색판별법을 이용한 콘크리트의 표면염화물량 산정 및 매립철근의 건전도 평가 (Calculation of the Surface Chloride and Estimation for the Soundness of Embedded Rebar by Using Colorimetric Distinction Method)

  • 이문환;이진우
    • 콘크리트학회논문집
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    • 제15권6호
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    • pp.794-801
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    • 2003
  • 건축물의 요구성능에 대한 관심이 점차 높아짐에 따라 건축물의 성능평가를 통하여 사용연한을 예측하고 건축물의 수명을 연장시키거나, 새롭게 재구성하려는 노력이 계속되고 있다. 그러나 아직 염화물에 의한 성능저하 평가에 있어 그 비용의 문제와 평가과정의 전문성 등의 많은 난점을 가지고 있다. 본 연구는 콘크리트 내부의 염화물침투 깊이를 측정하는 간이적 방법인 비색판별법을 이용하여 염화물에 의한 성능저하평가를 하고자 비색판별법의 적용가능성을 검토 후 변색위치의 침투염화물량을 정량분석하였다. 또한, 이를 이용하여 콘크리트 표면염화물량을 산정하고 철근위치 염화물량의 예측 및 한계염화물량에 도달시간 예측의 실용적 평가수단을 제시하였다. 염화물에 의한 철근콘크리트 구조물의 성능평가에 있어 간이평가 수단인 비색판별법의 활용에 따라 샘플수의 다량확보를 통한 신뢰수준의 향상을 이룰 수 있을 것으로 사료된다.

4-(p-Nitrobenzyl)pyridine의 색깔반응을 이용한 미생물 epoxide hydrolase의 활성 평가 (Evaluation of Microbial Epoxide Hydrolase Activity Based on Colorimetric Assay Using 4-(p-nitrobenzyl) Pyridine)

  • 김희숙;이은열
    • 생명과학회지
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    • 제15권3호
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    • pp.332-336
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    • 2005
  • 4-(p-Nitrobenzyl)pyridine (NBP)의 색깔변화를 이용한 epoxide hydrolase활성 측정법을 사용하여 다양한 미생물 세포유래의 epoxide hydrolase 활성을 측정하고 평가하였다. Epoxide hydrolase의 가수분해 반응에 의해 분해되고 남은 에폭사이드 기질에 의한 NBP alkylation 반응을 통한 색깔변화로 손쉽게 epoxide hydrolase 활성을 측정할 수 있었다. NBP 반응에서 triethylamine을 첨가하여 색깔반응 효율을 높일 수 있었으며, 10mM styrene oxide에 대한 최적 세포 사용량은 12.5mg/ml로 결정하였다. 본 연구에서 얻은 colorimetric assay조건에서 대용량의 미생물 후보군집으로부터 유용한 epoxide hydrolase를 가지는 신규 미생물을 효율적으로 선별할 수 있을 것으로 기대된다.

플렉소 잉크 및 유기안료의 6가 크롬 함량 측정 방법에 관한 연구 - 알칼리 분해 및 비색측정법 - (Study on the Quantitative Analysis Methods of Hexavalent Chromium in Flexography Inks and Organic Pigments - Alkaline digestion and Colorimetric Measurement -)

  • 김진우;윤혜정;이학래
    • 펄프종이기술
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    • 제41권4호
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    • pp.33-42
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    • 2009
  • This study was performed to evaluate the hexavalent chromium content in flexography inks and organic pigments used as colorants for the ink. The digestion of sample was carried out under alkaline condition, and the content of soluble hexavalent chromium extracted from samples was determined by UV-visible colorimetric analysis method after alkaline digestion. Duplicate sampling system to obtain two digested samples, was applied in this study. To determine the hexavalent chromium content in the flexography ink and organic pigment colorimeteric analysis was employed. Because the organic pigment is the main ingredient of flexography ink it is important to remove the errors associated with the colorant of the ink in colorimetric determination. The duplicate sampling system allowed us to correct the errors associated with the colorimeteric measurement. The additional filtration was found an essential step to exclude colorimeteric error derived from the various precipitates. The soluble hexavalent chromium content in flexography inks was generally less than 5 ppm. Yellow, violet and some magenta colors showed higher soluble hexavalent chromium contents. The content of hexavalent chromium in organic pigments was greater than flexography inks, and yellow, violet and some magenta pigments contained greater amount of hexavalent chromium, which indicated that the hexavalent chromium in inks derives from the colorants. The soluble hexavalent chromium content in linerboards were below 1 ppm, and no hexavalent chromium was detected in UKP. Results suggested that flexography ink is the main factor to cause hexavalent chromium in linerboard and organic pigments.

Comparison of different colorimetric assays and application of the optimized method for determining the liberated fluoride contents in various tea extracts

  • Le-Thi Anh-Dao;Do Minh-Huy;Nguyen-Ho Thien-Trang;Nguyen Cong-Hau
    • 분석과학
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    • 제37권2호
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    • pp.87-97
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    • 2024
  • The appropriate intake of fluoride (F-) is beneficial to human health; however, the over-consumption can result in various potentially harmful effects. This study compared different colorimetric reagents, i.e., aluminium-xylenol orange (Al-XO), zirconium-xylenol orange (Zr-XO), and zirconium-alizarin red S (Zr-ARS), for fluoride measurements by the UV-Vis, in terms of reaction mechanisms, method sensitivity, and interferences from aluminium and ferric ions. The colorimetric procedures were optimized, and the analytical methods were evaluated. The goodness of linearity (R2 > 0.998) was obtained for all three assays within the concentration range of 1.0-20.0 mg/L fluoride in deionized water, in which the method sensitivity followed the descending order of Zr-XO > Al-XO > Zr-ARS. The Zr-XO was applied for determining the fluoride in different tea extracts in water (90 ℃ and 60-minute-brewing) and black tea demonstrated the highest fluoride content (3.0-3.6 mg/L). The effects of brewing time and temperature on the release of fluoride in the tea extracts were also investigated, indicating these are critical factors for the fluoride extraction. This study highlighted the application potentials of the UV-Vis measurement as a simple, convenient, and cheap analytical approach and discussed different colorimetric reagents used for fluoride determination in tea extracts in the context that the UV-Vis spectrophotometers are commonly equipped in most laboratories.

측정각도를 고려한 액정교정기법의 개선 (Improvement of Calibration Method of Thermochromic Liquid Crystal Reflecting Measurement Angle)

  • 윤순현;심재경;우창수
    • 대한기계학회논문집B
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    • 제24권2호
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    • pp.188-194
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    • 2000
  • Thermochromic liquid crystal reflect a unique color at even temperature. Therefore, they have been successfully applied to non-intrusive heat transfer research. Hue capturing method is widely used in the quantitative measurement from the TLC image. However it is affected by several measurement conditions. The distances of camera and light source have little influence on the color, but the value of hue is seriously affected by the measurement angle. In this study, the hue capturing method is improved by considering the effect of measurement angle. This improved calibration method can diminish the misreading of temperature caused by curvature of test surface.

몇가지 중금속이 배양 섬유모세포에 미치는 세포독성에 관한 연구

  • 정연태;박승택;김정중;최봉규;우원홍;위인선
    • Toxicological Research
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    • 제8권1호
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    • pp.119-129
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    • 1992
  • Present study was carried out to investigate the cytotoxicity of cadmium, chromium and mercury on cultured rat fibroblasts. The colorimetric assays of neutral red (NR) and tetrazolium MTT, the measurement of total content of protein and electron microscopic studies were performed on the fibroblasts cultured in the media containing various concentrations of cadmium, chromium and mercury. The results are as follows' 1. In cadmium-treated group, the NR and MTT values were dose-dependent increase. The NR90, NR50, MTT90 and MTT50 values of cadmium were 0.2mM, 19.5mM 1.0mM and 60.0mM, respectively.

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국산 오프셋 인쇄물의 색재현 특성에 대한 평가(II) - 색차 측정법에 의한 평가 - (The Color Reproduction Property Evaluation of Domestic Offset Prints (II) - Using Colorimetric Method -)

  • 김진영;강상훈
    • 한국인쇄학회지
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    • 제24권1호
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    • pp.45-57
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    • 2006
  • Offset lithography is the most widely used printing process in domestic printing industry, but there are no industry-wide specifications for press control to assure consistent quality across printing plants. As printing becomes more of a commodity and less of an art, it is necessary to develop a print quality specification standard suitable for Korea offset printing field. This study aims to contribute to improving the quality of domestic offset color prints by comparative study on color reproduction properties of 2 kinds of domestic process color ink composed of C(cyan), M(magenta), Y(yellow) and K(black) and of 3 kinds of domestic coated paper, using colorimetric color measurement method.

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오존간이 측정기에 대한 연구(II)-간섭현상 및 현장 적용성 (A Study on Ozone Passive Sampler (II) -Interference and Field Application)

  • 정상진;양희준
    • 한국대기환경학회지
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    • 제22권6호
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    • pp.904-911
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    • 2006
  • Passive sampler is simple and inexpensive measuring equipment for air pollution. In this study we developed colorimetric ozone passive sampler which was coated a colorimetric reagent to a filter substrate. Interference of criteria all pollutant ($SO_2,\;NO_2,\;CO$) on ozone passive sampler was investigated through experimental chamber. Field measurement of developed ozone passive samper was compared automatic sampler result in Suwon area. The results shows interference of $SO_2$ gas is larger than other gases. Developed passive sampler can be roughly applicable to wind speed range from 0.8 to 3.3 m/s, temperature range from 3.9 to $27.5^{\circ}C$ and relative humidity range from 48 to 84%.