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http://dx.doi.org/10.5352/JLS.2005.15.3.332

Evaluation of Microbial Epoxide Hydrolase Activity Based on Colorimetric Assay Using 4-(p-nitrobenzyl) Pyridine  

Kim Hee Sook (Department of Food Science and Technology, Kyungsung University)
Lee Eun Yeol (Department of Food Science and Technology, Kyungsung University)
Publication Information
Journal of Life Science / v.15, no.3, 2005 , pp. 332-336 More about this Journal
Abstract
Epoxide hydrolase activities of various microbial cells were analyzed by colorimetric assay based on alkylation of epoxides with 4-(p-nitrobenzyl)pyridine (NBP). The epoxide hydrolase activity was determined by measuring the decrease of color intensity at 560 nm due to the decrease of styrene oxide substrate by epoxide hydrolase-catalyzed hydrolysis reaction. The experimental conditions of NBP colorimetric assay were optimized for the efficient measurement of epoxide hydrolase activities from various microbial cells.
Keywords
assay; biocatalyst; epoxide hydrolase; 4-(p-niteobenzyl)pyridine; styrene oxide;
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