• Title/Summary/Keyword: Colocasia esculenta

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The anti-inflammatory effect of Colocasia esculenta water extract on mouse ear edema models induced by TPA

  • Kang, Dong Woo;Choi, Soo Cheol;Kang, Jeong Eun;Park, Ji Sun;Lee, In Ah
    • Journal of People, Plants, and Environment
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    • v.24 no.1
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    • pp.53-62
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    • 2021
  • Background and objective: Dermatitis is a chronic disease accompanied by such symptoms as itching and dry skin. The environment and diet can aggravate dermatitis, so attention to skin care is essential. Colocasia esculenta is used in various manners and for different purposes, including with regard to inflammation, aging, and the digestive system. The anti-inflammatory effect of Colocasia esculenta water extract was confirmed using RAW 264.7 macrophages with regard to male ICR mice. Methods: In the case of the ICR mice, 5% 12-O-Tetradecanoylphorbol-13-acetate (TPA) was used to cause inflammation for 7 days, and 100 μL of Colocasia esculenta water extract and panthenol were administered orally for 10 days. In addition, RT-PCR, NO, ELISA was conducted. Results: As a result of reverse transcription polymerase chain reaction (RT-PCR) using RAW 264.7 macrophages stimulated with lipopolysaccharide (LPS), it was found that Colocasia esculenta water extract reduced the expression of inflammatory cytokines. As a result of hematoxylin and eosin (H&E) staining using mouse ear tissue, Colocasia esculenta water extract reduced ear thickness and showed an effect of suppressing ear edema. In addition, compared to the TPA-treated group, the Colocasia esculenta extract-treated group had reduced nitric oxide (NO) production by 18.23 μM and IL-13 production decreased by 136.55 pg/ml. Conclusion: Colocasia esculenta water extract has been shown to be effective in lowering inflammatory cytokine production. These results suggest that Colocasia esculenta water extracts can be used as natural products to treat dermatitis.

Genotype Difference of Plant Regeneration from Dormant Bud Culture in Colocasia esculenta Schott.

  • Rha, Eui-Shik;Yoo, Nam-Hee;Kim, Hyun-Soon
    • Plant Resources
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    • v.2 no.2
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    • pp.65-68
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    • 1999
  • This study was conducted to obtain the basic breeding information of Colocasia esculenta Schott. Effect of supplemental plant growth regulators and genotype difference were investigated on dormant bud tissue for proliferation. The plant regeneration ratio, plant height and root length were the best upon mixed treatment of 0.8mg/L IAA and 2.0mg/L zeatin. Both leaf weight and root weight were heavy upon culture in a dark condition. The leaf and root weights were heaviest in 6Pie sucrose concentrations. In several collected area the heaviest one was Binnangxin and then in the order of Suwon, Wanju and Puan. Genotype differences of tuber diameter and tuber weight were found in Suwon. Tuber weight was found in the order of Suwon (862mg) >Wanju(723mg) >Puan(649mg) >Binnangxin (424mg).

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Characterization of a Korean Isolate of Dasheen mosaic virus Isolated from Taro (Colocasia esculenta Schott) in Korea

  • Kim, Min-Kyu;Kwon, Soon-Bae;Yoon, Ju-Yeon;Ryu, Ki-Hyun;Heo, Su-Jeong;Hong, Jeong-Ki;Kim, Kyung-Hee;Park, Jang-Kyung
    • The Plant Pathology Journal
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    • v.20 no.2
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    • pp.135-141
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    • 2004
  • A filamentous virus was isolated from taro (Colocasia esculenta Schott) showing mosaic and chlorotic feather-ing symptoms in Chuncheon, Gangwon province in 2002. Based on ELISA, its appearance in electron microscope, serological relationships, and RT-PCR using specific primer and nucleotide sequence analysis of the CP gene, the isolated virus was identified as Dasheen mosaic virus (DsMV) and designated as Korean isolated (DsMV-Kr). DsMV was not serologically related to Zantedeschia mosaic virus (ZaMV), which has been reported to infect an Araceae plants. Since the coat protein revealed electrophoretic heterogeneity, about 42 kDa, 39 kDa and 31 kDa by SDS-PAGE, an improved purification method was established for the production of antisera against DsMV-Kr. The purification method used in this study may be effectively applied to the purification of other filamentous viruses.

The Effect of Activated Charcoal on Growth and Yield in Taro, Colocasia antiquorum var. esculenta Engl. (토란(Colocasia antiquorum var. esculenta Engl.)의 생육과 생산성에 미치는 활성탄의 효과)

  • 최성규;박영태;윤경원
    • Korean Journal of Plant Resources
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    • v.15 no.3
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    • pp.293-297
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    • 2002
  • This study was conducted to investigate the effect of activated charcoal on growth and yield of taro in different degree of activated charcoal concentration. The results obtained are summarized as follows. Stem length was shown the longest in activated charcoal of 10%∼20% , but shorter in control. Also stem diameter was shown the thickest in activated charcoal of 10%∼20%, but thinner in control. Length and thickness of main root and thickness of main root were good in activated charcoal of 10∼20% Quality and yield of taro was the highest in activated charcoal of 10∼20% . Therefore, optimum consistency of activated charcoal was 10∼20%.

Fertilizer Use Efficiency of Taro (Colocasia esculenta Schott) and Nutrient Composition of Taro Tuber by NPK Fertilization

  • Lee, Ye-Jin;Sung, Jwa-Kyung;Lee, Seul-Bi;Lim, Jung-Eun;Song, Yo-Sung;Lee, Deog-Bae
    • Korean Journal of Soil Science and Fertilizer
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    • v.49 no.4
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    • pp.388-392
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    • 2016
  • The objectives of fertilizer recommendation are to prevent the application of excessive fertilization and to produce target yields. Also, optimal fertilization is important because crop quality can be influenced by fertilization. In this study, yields and fertilizer use efficiency of Taro (Colocasia esculenta Schott) were evaluated in different level of NPK fertilization. N, P and K fertilizer application rates were 5 levels (0, 50, 100, 150, 200%) by practical fertilization ($N-P_2O_5-K_2O=180-100-150kg\;ha^{-1}$), respectively. In the N treatment, the yields of Taro tuber were about $33Mg\;ha^{-1}$ from 90 to $360kg\;ha^{-1}$ N fertilization. However, the ratio of tuber to total biomass decreased with increasing N fertilization rate. In the P and K treatments, yields of Taro tuber were the highest at $150kg\;ha^{-1}$ fertilization. Fertilizer use efficiency was decreased by increase of N and K fertilization. Crude protein of Taro tuber was the highest at practical fertilization. Sucrose content of tuber was influenced by phosphate application.

The Effect of IAA on $Colocasia$ $esculenta$'s Growth and Morphogenesis (IAA가 토란의 생장 및 형태 발생에 미치는 영향)

  • Kim, Young-Eun;Lee, Joon-Sang
    • Korean Journal of Environmental Biology
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    • v.29 no.2
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    • pp.92-97
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    • 2011
  • Morphological and physiological differences of $Colocasia$ $esculenta$ were investigated in the cultivation of hydorphonic and soil culture. $C.$ $esculenta$ grown in Hoa.+IAA (indole-acetic acid) showed higher growth activity representing 9%, 32%, 38% and 60% than those of the cultivation of vermiculate, Hoagland solution, soil and water, respectively. In case of $F_v/F_m$ ratio experiments, the value $F_v/F_m$ of $C.$ $esculenta$ cultivated in the water showed 0.55 after 6 weeks. $F_v/F_m$ values of $C.$ $esculenta$ cultivated in Hoagland+IAA, vermiculate and soil were between 0.84 and 0.80 indicating $F_v/F_m$ values were about 45% higher than that of $C.$ $esculenta$ cultivated in the water. Diffusion resistance was 45~35% lower in $C.$ $esculenta$ grown in Hoa.+IAA solution than that of $C.$ $esculenta$ grown in water only after 5 and 6 weeks. Therefore, the high standing levels of the growth rate, fluorescence activity and transpiration rate were Hoa.+IAA, vermiculate, Hoagland, soil and water. The distinct morphological differences of $C.$ $esculenta$ cultivated in hydorphonic and soil culture were the appearance of the seed corm and root hair. The development of seed corm was well established in soil culture but the corm in hydorphonic was slowly hydrolyzed and then disappeared. The fibrous root systems of hydorphonic were very well distinguishable compared with that in soil culture. Outstanding results of this experiment were appeared in $C.$ $esculenta$ which was cultivated in the field provided with enough mineral nutrition, organic fertilizers and compound fertilizers. The most height taros were almost 2m and the numbers of seed corm were 30~40 after 7 months.

The genetic structure of taro: a comparison of RAPD and isozyme markers

  • Sharma, Kamal;Mishra, Ajay Kumar;Misra, Raj Shekhar
    • Plant Biotechnology Reports
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    • v.2 no.3
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    • pp.191-198
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    • 2008
  • Germplasm characterization and evolutionary process in viable populations are important links between the conservation and utilization of plant genetic resources. Here, an investigation is made, based on molecular and biochemical techniques for assessing and exploiting the genetic variability in germplasm characterization of taro, which would be useful in plant breeding and ex situ conservation of taro plant genetic resources. Geographical differentiation and phylogenetic relationships of Indian taro, Colocasia esculenta (L.) Schott, were analyzed by random amplified polymorphic DNA (RAPD) and isozyme of seven enzyme systems with specific reference to the Muktakeshi accession, which has been to be proved resistant to taro leaf blight caused by P. colocasiae. The significant differentiations in Indian taro cultivars were clearly demonstrated by RAPD and isozyme analysis. RAPD markers showed higher values for genetic differentiation among taro cultivars and lower coefficient of variation than those obtained from isozymes. Genetic differentiation was evident in the taro accessions collected from different regions of India. It appears that when taro cultivation was introduced to a new area, only a small fraction of genetic variability in heterogeneous taro populations was transferred, possibly causing random differentiation among locally adapted taro populations. The selected primers will be useful for future genetic analysis and provide taro breeders with a genetic basis for selection of parents for crop improvement. Polymorphic markers identified in the DNA fingerprinting study will be useful for screening a segregating population, which is being generated in our laboratory aimed at developing a taro genetic linkage map.

Structural elucidation of immuno-stimulating polysaccharide, galactomannan isolated from Colocasia esculenta (토란으로부터 분리한 면역활성 다당 galactomannan의 구조적 특성 규명)

  • Lee, Hee-Won;Shin, Kwang-Soon
    • Korean Journal of Food Science and Technology
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    • v.52 no.6
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    • pp.595-603
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    • 2020
  • To elucidate the structure-function relationship of polysaccharides obtained from Colocasia esculenta, the immuno-stimulating polysaccharide, CE-4a was purified to homogeneity from the crude polysaccharide (CE) extracted from the corms of C. esculenta by two subsequent column chromatographies using DEAE-Sepharose FF and Sephadex G-100, and analysis of their immuno-stimulatory activities and structure were conducted. CE-4a showed an increase in anti-complementary activity in a dose-dependent fashion. The molecular mass was estimated to be 182.4 kDa, which mainly consisted of galactose (43.5%) and mannose (18.2%). Methylation analysis indicated that CE-4a comprised at least 10 different glycosyl linkages, such as terminal Galp, 3-linked Galp, and 4-linked Manp, as well as a characteristic linkage, 2,4,6-branched Manp residue. To analyze the fine structure of CE-4a, it was sequentially digested using endo-α-(1→4)-polygalacturonase, exo-α-galactosidase and endo-β-1,4-D-mannanase. These analyses suggested that CE-4a is to be a highly branched galactomannan with a (1→4)-mannan backbone and galactopyranosyl oligosaccharide side chains.