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Phenolic Compounds Content and DPPH, ADH, ALDH Activities of Mungbean Sprout Based on Growth Temperature (녹두나물 재배온도에 따른 페놀화합물 함량과 DPPH, ADH 및 ALDH 활성)

  • Kim, Dong-Kwan;Son, Dong-Mo;Chon, Sang-Uk;Lee, Kyung-Dong;Kim, Kyong-Ho;Rim, Yo-Sup
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.54 no.1
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    • pp.1-6
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    • 2009
  • This study is to analyze the effects of the growth temperature of mungbean sprouts ($15{\sim}30{\pm}1^{\circ}C$) on the yield ratio, content of phenolic compounds and DPPH (1,1-diphenyl-2-picrylhydrazyl), ADH (alcohol dehydrogenase), ALDH (aldehyde dehydrogenase) activities of the sprouts. When the growth temperature of mungbean sprouts was higher, the yield ratio of the sprouts was higher while the hard seed rate was lower, but $25{\pm}1^{\circ}C$ and $30{\pm}1^{\circ}C$ showed no regular tendency. The content of the total phenol from the ethanol extract of the sprouts was higher in the growth temperature of $15{\pm}1$, $20{\pm}1$, and $25{\pm}1^{\circ}C$, while the content of total flavonoid was higher in the growth temperature of $15{\pm}1$, and $20{\pm}1^{\circ}C$. The DPPH radical scavenging activity of the ethanol extract of the sprouts was higher when the growth temperature was lower, while the activity of ADH and ALDH showed no regular tendency according to the growth temperature. Considering the yield ratio, content of phenolic compounds, biological activities of mungbean sprouts, the optimum cultivation temperature of mungbean sprouts may be $20{\sim}25^{\circ}C$.

Chondrogenic Differentiation of Human Mesenchymal Stem Cells on a Patterned Polymer Surface (패턴된 폴리머를 이용한 중간엽줄기세포의 연골 분화)

  • Heo, June Seok
    • Korean Journal of Clinical Laboratory Science
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    • v.47 no.3
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    • pp.117-124
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    • 2015
  • Mesenchymal stem cells (MSCs) are an attractive tool in tissue engineering as they have the required potential to treat injured articular cartilage. UV-exposed DTOPV (S-triazine bridged p-phenylene vinylene) is a biocompatible and fluorescent polymer with a hydrophilic surface. Previous studies have demonstrated that the surface wettability and hydrophilicity play critical roles in regulating cell adhesion and proliferation. The objective of this study was to improve the potential of in vitro MSC differentiation into Chondrocytes using DTOPV. MSCs were cultured on two different substrates: (1) tissue culture polystyrene (TCPS) as a reference and (2) UV-exposed and patterned DTOPV films. Chondrogenesis of MSCs was induced for two weeks on TCPS and DTOPV in the presence of an induction medium containing transforming growth factor (TGF)-${\beta}3$. Interestingly, the MSCs on TCPS adhered and spread, while those on DTOPV tended to form aggregates within several days. The cells cultured on DTOPV for two weeks had a round morphology, with stronger Safranine O staining of the extracellular matrix than that of the cells cultured on TCPS. Also, Type II collagen gene was significantly expressed in cells induced on DTOPV. These results indicate that chondrogenic differentiation of MSCs proceeds more rapidly on DTOPV than on TCPS. Therefore, in cartilage tissue engineering, DTOPV could be used to induce effective chondrogenic differentiation of MSCs.

Change of Mitochondrial Biogenesis Genes on Regular Exercise Training in Adipocytes of Ovariectomized Rats Fed on High Fat Diet (규칙적 운동이 고지방식이 난소절제흰쥐의 지방세포에서 미토콘드리아 생합성 유전자들의 변화)

  • Lee, Jin
    • Journal of Life Science
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    • v.21 no.7
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    • pp.997-1003
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    • 2011
  • Menopause and obesity are associated with metabolism. The purpose of this study was to examine the changes of PPAR${\gamma}$, PGC-1(${\alpha},\;{\beta}$), NRf-1 and TFAM mRNA and mitochondria biogenesis in adipocytes and investigate the effect of swimming exercise for 6weeks on ovariectomized rats. Rats were randomly assigned to 3 groups: (1) ovariectomized rats fed with a control diet (C, n=4), (2) ovariectomized rats fed with high fat diet (H, n=4), and (3) ovariectomized rats trained to exercise and fed with high fat diet (H+EX, n=4). Exercise was performed by swimming for 5 days/wk, with a progressive increase in exercise over the course of 6 weeks. Results showed that the fat tissue weight in the H group was markedly increased (p<0.01) compared to other groups, however, regular exercise significantly decreased fat weight. The PPAR-${\gamma}$ (p<0.05), PGC-$1{\alpha}$ (p<0.01), -$1{\beta}$ (p<0.05), NRf-1 (p<0.01) and TFAM (p<0.05) mRNA expression in the adipocytes of H+EX were higher than in the H group. These results suggest that regular exercise for 6 weeks might exert positive effects by increasing PPAR-${\gamma}$, PGC-1 (${\alpha},\;{\beta}$), NRf-1 and TFAM mRNA expression and mitochondria in adipocytes. Thus, regular exercise may be helpful in the improvement of mitochondria biogenesis function in obese, ovariectomized rats.

A Study on Quick Quantitative Analyzing Method of Sorbic and Benzoic Acids in Doenjang by Gas Chromatography (기체크로마토그래피에 의한 시판 된장중의 소르빈산과 안식향산의 신속정량법 연구)

  • Park, Kwang-Ja;Lee, Si-Kyung;Kim, Nam-Dae;Cho, Nam-Ji
    • Korean Journal of Food Science and Technology
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    • v.31 no.2
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    • pp.285-292
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    • 1999
  • An analytical method applicable to quality control and its optimum conditions were studied for rapid and efficient analysis of sorbic and benzoic acids used as preservatives in the commercial soybean paste. In gas chromatographic analysis of sorbic and benzoic acids, the application of HP-FFAP (acid modified polyethylene) wide bore column improved the separation ability significantly. By setting the oven temperature of GC to $200^{\circ}C$, the total elapsed time for quantitative analysis was also reduced to the level required in using packed column. By extracting sorbic and benzoic acids from soybean paste with an automatic steam distillation device, the elapsed time for analysis was reduced by 80% more compared to using conventional steam distillation method. The recoveries of sorbic and benzoic acids by the automatic steam distillation were 98.1% and 99.9%, respectively. The sorbic acid was found in 3 samples of 14 commercial soybean pastes, of which contents were $466{\sim}530ppm$, while $0.3{\sim}4.4ppm$ of benzoic acid was found in all the samples.

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Mark Chagall's Paintings Transferred into Contemporary Korean Poems: Youngtae Kim's Anthology, Winter in the Village of Jews, Chunsoo Kim's "Snow Falling on the Village of Chagall," and Sunghun Lee's Anthology, Poetic Anthology of Chagall (한국 현대시에 수용된 마르크 샤갈 그림 - 김영태 시집 "유태인 사는 마을의 겨울" 김춘수 시 "샤갈의 마을에 내리는 눈" 이승훈 시집 "시집 샤갈$\lcorner$에 수용된 샤갈의 그림세계)

  • 윤호병
    • Lingua Humanitatis
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    • v.1 no.1
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    • pp.141-157
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    • 2001
  • In his discussion of some desirable tuning points in comparative literary studies, Henry H. H. Remark has emphasized the importance of literary approach to other forms of art. Understanding the significance of such a method of comparative literature, the present study focusses on three contemporary Korean poets who have transferred Mark Chagall′s paintings into their poetry: Youngtae Kim, Chunsoo Kim, and Sunghun Lee. They are usually evaluated as surrealist/modernist in our literary circles. In transforming Chagall′s paintings into his poems, Youngtae Kim has incorporated a variety of surrealist mosaic techniques such as montage and collage. The resultant peculiarity of his poetry makes it hard to lay bare the correspondence or similarities between his poetic world and the world of Chagall′s artistry. It is nonetheless possible to see how Kim, as a poet and painter, had interpreted Chagall′s world with a bird′s-eye view of it. Chunsoo Kim′s "Snow Falling on the Village of Chagall" relates specially to one of Chagall′s paintings, "I and My Village." The present study has taken notice of this correlation in sorting out some basic elements of poetic transfiguration. One of the techniques employed in the poem under discussion is that of juxtaposing the Russian village of Chagall and the Korean village the poet visualizes, with the effect of putting two national traditions in contrast. A reading of the poem reveals that it is not so much the result of a detailed analysis of the painting as a revival of its lingering impression as a whole. In Sunghun Lee′s poetry, surrealist techniques are again a hallmark. But his method of transferring the images of the paintings into his poems falls somewhere between those of Youngtae Kim′s and Chunsoo Kim′s: it is akin to the ′bird′s-eye method′ of the former and shares the impressionistic touch with the latter, but at the same time Lee is analytical by disposition and opts for concrete descriptions. ′Love,′ ′farm,′ and ′time′ are the keywords that are brought under discussion in the present study. There is a growing demand in the current international comparative literary studies for broadening the area of comparative literature. This study hopes to be a small contribution to endorsing the importance of comparative approach to fine arts.

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Anti-aging Cosmetic Application of Novel Multi-herbal Extract Composed of Nelumbo nucifera Leaves, Saururus chinensis and Orostachys japonica (하엽, 삼백초 및 와송으로 구성된 식물복합추출물의 항노화 화장품 소재로서의 응용성 연구)

  • Baik, Minyoung;Kim, Jun Hyeong;Lee, Dae Woo;Hwang, Jae Sung;Moon, Eunjung
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.43 no.2
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    • pp.93-102
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    • 2017
  • In this study, various plant extracts were screened for the effective and safe skin care ingredient according to the antioxidant activity assay guidelines. We selected an optimized combination herbal extract, Charmzone extract (CZE), which composed of Nelumbo nucifera leaves, Saururus chinensis and Orostachys japonica. CZE exerted free radical scavenging activity. It reduced reactive oxygen species formation and increased total antioxidant capacities in human keratinocyte (HaCaT) and normal human dermal fibroblasts (NHDF). CZE also leaded procollagen type 1 secretion in NHDF and decreased cellular melanin contents in B16F10. The production of nitric oxide was decreased by CZE in lipopolysaccharide-stimulated RAW264.7 in a dose dependent manner. Therefore, it is concluded that CZE can be applied for naturally derived anti-aging functional skin care ingredient for anti-oxidation, wrinkle enhancement, whitening, anti-inflammation and wound healing of skin.

Effects of Adding Bamboo leaves Powder on the Quality of Jeolpyon (댓잎분말을 첨가한 댓잎절편의 일반성분 및 품질특성)

  • Kim, Duk-Han;Hwang, Su-Jung
    • Korean journal of food and cookery science
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    • v.22 no.6 s.96
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    • pp.869-874
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    • 2006
  • This study investigated the effect of bamboo leaf powder, added at content of 0%, 3%, 6%, 9% and 12%, on the quality and sensory characteristics of rice cake imprinted with flower pattern (Ed-there is no respecitive comparison here) The chemical composition as a whole was 3.07${\pm}$0.12% of moisture, 5.87${\pm}$0.02% of crude protein, 2.52${\pm}$0.05% of crude fat, 23.70${\pm}$0.07% of crude fiber and 7.73${\pm}$0.02% of crude ash. With increasing amount of added bamboo leaf powder, the hardness and chewiness of the cake were significantly increased(p<0.05), while the cohesiveness, springiness and adhesiveness were decreased. In chromaticity, the L-value, the indicator of brightness, was significantly decreased with increasing bamboo leaf powder content. The a-value, the indicator of redness, was significantly lower than the chlorophyll color (p<0.05). The b-value, the indicator of yellowness, was increased indirect proportion to the content. In sensory test, the sensory characteristics such as color, flavor, taste and chewiness were all stronger with increasing powder content, whereas the softness and dampness were weaker. The greatest overall satisfaction was achieved by the sample group of cake with 6% preference added powder.

Quality characteristics of tofu coagulated by apricot juice (살구즙을 응고제로 첨가한 두부의 품질특성)

  • Lee, Sun-Ju;Chung, Eio-Sook;Park, Geum-Soon
    • Korean journal of food and cookery science
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    • v.22 no.6 s.96
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    • pp.825-831
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    • 2006
  • This study investigated the utilization of apricot as a coagulants for tofu manufacture, and compared the quality characteristics and shelf-life of tofu made by A2(2% apricot juice), A4(4% apricot juice), A6(6% apricot juice), A8(8% apricot juice) in comparison with those of C(G.DL). A2 tofu(323.00 g) showed the highest yield of tofu and the tofu yield decreased with increasing apricot juice content. In color, the lightness of tofu was decreased with increasing apricot juice content, while redness and yellowness were increased. The hardness of tofu coagulated with apricot juice was higher than that of the coagulated C(G.DL) and the springiness of A6 and A8 was higher than that of C(G.DL) while that of the other tofus was lower than C(G.DL). The brittleness of tofu was increased with increasing apricot juice content. The pH value of tofu coagulated with apricot juice was increased during storage at $0^{\circ}C$ and the acidity was decreased. Two percent apricot juice tofu had good overall preference.

Monitoring of Hydrogen Sulfide in Anaerobic Co-digestion of Swine Manure and Food Waste (돈분 및 음식물쓰레기 혼용 혐기소화 시 황화수소 가스 모니터링)

  • Shin, JoungDu;Sung, Shihwu;Kim, Hyunook;Kim, SamcCeun;Lee, MyoungSun
    • Journal of the Korea Organic Resources Recycling Association
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    • v.16 no.4
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    • pp.43-49
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    • 2008
  • Objective of this study was to monitor the hydrogen sulfide production rate and concentration in anaerobic co-digestion of swine manure and food waste for biogas production in order to alternate the petroleum based energy. Anaerobic co-digestion for biogas production was performed in serum bottles at 2% volatile solids (VS) concentration and various mixing ratios of two substrates(swine manure: food waste = 100:0 ~ 0:100). Although hydrogen sulfide production rates were varied with digestion periods at different treatments, it was observed that hydrogen sulfide produced in the swine manure alone was lower at 2.4 fold than that of food waste. For effects of hydrogen sulfide concentration in the different mixing ratios of swine manure to food waste, the higher food waste ratio the higher hydrogen sulfide concentration. Also its average concentrations were varied from 0.1452% in the swine waste only to 0.3420% in the food waste alone. For the composition ratio of bio-gas in their anaerobic co-digestion, it appeared that there was 53.2% of $CH_4$, 23.9% of $CO_2$, 0.3% of $H_2S$ and 22.7% of miscellaneous gases including moisture.

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Quality Characteristics of Apricot Sulgidduk by the Saccharides Assortment (당 종류별 살구설기떡의 품질특성)

  • Shin, Young-Ja;Park, Geum-Soon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.36 no.2
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    • pp.233-240
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    • 2007
  • The purpose of this study was to evaluate the effect of different saccharides on the quality of apricot sulgidduk. Appearance color of honey-added group as compared to other samples in apricot sulgidduk were preferable and scanning electron microscopy exhibited smaller pores. pH and acidity of white-added group immediately after preparation were higher than those of other groups. Higher moisture contents of honey-added groups during storage were found but total bacterial counts immediately before storage were 1 log cycle lower than that of other samples. The highest 'L' values were found for white sugar added group but the highest 'a' and 'b' were for yellow sugar-added sample. In mechanical characteristics of apricot sulgidduk for 48 hrs during storage at $20^{\circ}C$, hardness, gumminess and brittleness were higher in oligosaccharide-added sample as compared to other samples, while cohesiveness and springiness were the highest in honey-added group. In sensory evaluation of the sulgidduk, honey-added group obtained the highest score in color, sweet taste, moisture, after swallowing and overall quality (p<0.05) These results suggest that apricot sulgidduk with honey can be used as commercially successful rice cakes.