• Title/Summary/Keyword: Cold-Storage

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Application of Slow-Freezing Cryopreservation Method for the Conservation of Diverse Potato (Solanum tuberosum L.) Genotypes

  • Zhao Mei-Ai;Dhital Shambhu P.;Fang Yi-Lan;Khu Dong-Man;Song Ye-Su;Park Eung-Jun;Kang Chang-Won;Lim Hak-Tae
    • Journal of Plant Biotechnology
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    • v.7 no.3
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    • pp.183-186
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    • 2005
  • Cryopreservation has been recognized as a practical and efficient tool for the long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of slow-freezing techniques on the cryopreservation of potato. In vitro plantlets of the potato genotypes of 'Atlantic', 'Superior’, 'Namseo', 'J138', and 'CTO5-5' were cold acclimated, and the excised axillary buds were precultured, osmoprotected, exposed to plant vitrification solution, frozen slowly to $-40^{\circ}C$ and then rapidly plunged into liquid nitrogen, thawed and finally plated on the regeneration medium. It was found that the higher the sucrose concentrations in the subculture medium of donor plantlets, the higher the survival rates of shoot tips after cryopreservation, and the highest survival (20%) was observed in the medium added with 0.25 M sucrose. As for the effect of cooling, $0.3^{\circ}C/min$ cooling speed showed the highest survival (25%). Different varieties showed different responses over different cryopreservation treatments. Survival rate was increased by slow-freezing technique method as compared with that of the basic cryopreservation method of vitrification alone in the diverse potato genotypes. Leaf and tuber morphologies of potatoes regenerated after cryopreservation using slow freezing technique were similar to those derived from the in vitro stock plantlets.

Effects of different drying methods on fermentation characteristics and viability of cold-adaptive yeast (저온 적응성 효모의 보존방법에 따른 균체의 생존율 및 발효특성)

  • Baek, Seong Yeol;Mun, Ji-Young;Yeo, Soo-Hwan
    • Food Science and Preservation
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    • v.24 no.7
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    • pp.1034-1042
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    • 2017
  • The quality characteristics of Yakju and survival rate of yeast were investigated by modifying the drying method for the cold adapted yeast strain Saccharomyces cerevisiae Y297 (SCY297). Viability and fermentation characteristics of the freeze-dried, air blast-dried, and liquid SCY297 cultures were compared after storing them at $25^{\circ}C$. In addition, 5% skimmed milk, ${\alpha}$-lactose, or trehalose was added as a protective agent for examining the effects of drying methods. During the 15-week storage period, the liquid and freeze-dried SCY297 cultures containing a protective agent showed a survival rate of 80%. However, the air blast-dried SCY297 culture showed 80% survival rate only in the skimmed milk supplemented group. Compared to the untreated cells, the acidity and amino acidity of Yakju prepared using freeze-dried or air blast-dried cultures of SCY297 increased by 2 fold and 5.7 fold respectively, while the alcohol content decreased by 5.07%. Compared to the untreated cells, the pH and amino acidity of Yakju prepared using the liquid culture of SCY297 increased by 1.5 fold and 2.5 fold respectively. Although the alcohol content decreased by 2.9%, decrease rate was lower than that observed for the freeze-dried and air blast-dried yeast cultures. Therefore, the results of this study showed that using a liquid starter culture was more advantageous than using the conventional solid culture.

Incidence of Microorganisms during Slaughtering Process of Pig (돼지 도축공정 중의 미생물 증감 추이)

  • Cha Seong-Kwan;Seo Mi-Young;Kim Myung-Ho;Kim Yun-Ji
    • Food Science of Animal Resources
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    • v.25 no.1
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    • pp.1-6
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    • 2005
  • To evaluate the microbiological quality of pork carcasses at different slaughtering process in large and small scale slaughtering houses, swabbing method was used to analyze microorganisms on the surface of pork belly in each process of before evisceration, after evisceration, before final wash, after final wash and in chilling. In autumn time, large scale slaughterhouse showed lower incidence of aerobic microorganisms (10²∼10³ CFU/㎠) than those of small scale slaughterhouse (10⁴∼10/sup 5/ CFU/㎠) during all processing lines. Samples from cold room of large scale slaughterhouse showed lower incidence of aerobic cells (10² CFU/㎠) than small scale slaughterhouse (10⁴ CFU/㎠). In winter and spring time, large scale slaughterhouse showed lower incidence of aerobic microorganisms than those of small scale slaughterhouse during the slaughtering process of before evisceration, after evisceration and before final wash, except spring samples from before final wash and chilling at cold room storage in spring time. After final wash, different sampling place of carcass such as belly, ham, jowl showed the different washing effect depending on the small and large scale slaughterhouse. After final wash, ham and belly had lower aerobic cell counts, but jowl had higher aerobic cell counts than each site before final wash.

Incidence of Microorganisms during Slaughtering Process of Cattle (소 도축공정 중의 미생물 증감 추이)

  • Cha Seong-Kwan;Kim Yun-Ji;Kim Myung-Ho;Shin Jeom-Ho;Lee Moo-Ha
    • Food Science of Animal Resources
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    • v.24 no.3
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    • pp.232-237
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    • 2004
  • This study was carried out to evaluate the microbiological quality of beef carcasses at different slaughtering process in large (>100 cattle/day) and small (<30 cattle/day) scale slaughtering houses. Swabbing method was used to analyze the incidence of microorganisms on brisket surface of beef carcasses in each process of after dehiding, after evisceration, before and final wash, and in cold room. In winter time, large scale slaughterhouse showed lower incidence of aerobic microorganisms (10$\^$0/∼10$^2$ CFU/$\textrm{cm}^2$) than those of small scale slaughterhouse (10$\^$0/-10$^3$ CFU/$\textrm{cm}^2$) during the slaughtering process of after dehiding, evisceration and before final wash. But samples from carcasses after final wash and in cold room storage showed no difference in aerobic cell counts between large and small scale slaughterhouse. In spring time, samples showed higher incidence of microorganisms by the log scale 1 than those of winter time in both of small and large scale slaughterhouse. After final wash, different sampling place in carcass such as rump, flank, brisket showed the different washing effect in both of small and large scale slaughterhouse. After final wash, samples from rump showed lower aerobic cell counts, but samples from flank and brisket showed higher aerobic cell counts than samples from each site before final wash.

Study on Development of a Corrugated Packaging Box for Cold-chain Distribution (I) - Characteristics of moisture absorption to corrugated fiberboard - (저온유통용 골판지 포장상자 개발에 관한 연구(I) - 골판지의 흡습특성 시험 -)

  • Lee, Won-Og;Yun, Hong-Sun;Jeong, Hoon;Lee, Hyun-Dong;Cho, Kwang-Hwan;Kim, Man-Soo
    • Food Science and Preservation
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    • v.10 no.3
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    • pp.297-301
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    • 2003
  • The hardness of corrugated packaging box decreased during storage period because of high humidity. Therefore, the strength-decreasing factor of corrugated packaging box had to be investigated to determine the compression strength of the corrugated packaging box. This study was conducted to define the characteristics of moisture absorption as well as compression strength of corrugated fiberboard. (1) The moisture content of the corrugated fiberboard was rarely influenced by the kinds of raw materials and water repellent. However, the moisture adsorption of the corrugated fiberboard was shown to be clearly affected by air temperature changes. (2) The moisture content of the corrugated fiberboard was significantly affected by relative humidity. The moisture adsorption of corrugated fiberboard was also greatly influenced by composition of raw materials rather than water repellent. The results indicated that the improvement of raw materials was more effective than the increase of water repellent of corrugated fiberboard to reduce the moisture adsorption of the corrugated fiberboard. (3) The vertical compression strength was significantly decreased with increasing relative humidity. The reduction rate of vertical compression strength was not charged with the increase of relative humidity, which was regardless of kinds of corrugated fiberboard. (4) For improving the strength of the corrugated packaging box, new technique should be found to develop better quality of raw materials. Moreover, the physical construction of corrugated packaging box should be clearly understood because the corrugated packaging box for the agricultural products was usually used for the circumstances of high humidity and low air temperature

Quarantine Pest Occurrence in Exporting Pear Fruits (Pyrus pyrifolia Nakai cv. Whangkeumbae) during Cold Storag (수출용 황금배의 저온저장 중 검역병해충 발생조사)

  • Lee, Ho-Ki;Woo, Chang-Nam;Namkoong, Seung-Bak;Seo, Yong-Sun;La, Jin-Ho;Kim, Young-Il;Kim, Ki-Gyung;Kim, Byung-Kee
    • Horticultural Science & Technology
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    • v.18 no.1
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    • pp.22-27
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    • 2000
  • National plant quarantine inspector of exporting country should inspect agricultural products according to the quarantine requisites of counterpart country when the commodities are shipped. Export of pear fruits was held because quarantine pests including Conogethes punctiferalis were found at the point of entry in Canada. In order to examine the problems in detail, the inspected pear fruits were stored at a low temperature for 43 days, almost the same period necessary for fruit sorting, quarantine procedures, and transportation by ship. Dead larvae of fruit moths, C. punctiferalis and Grapholita molesta were found during cold storage. Quarantine pathogens were not found, but non-quarantine ones such as Penicillium sp. was infected through wounds by bruises and stabs resulted from sorting procedures. Because of the wounding problem during export procedures, pear fruits with thin exocarp, such as fruits of P. pyrifolia Nakai cv. Whangkeumbae, are required more careful handling during fruit sorting and conveyance.

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Comparison of the Physicochemical Properties of Meat and Viscera of Dried Abalone (Haliotis discus hannai) Prepared using Different Drying Methods (건조방법에 따른 건조 전복 (Haliotis discus hannai)의 이화학적 특성 비교)

  • Park, Jeong-Wook;Lee, Young-Jae;Park, In-Bae;Shin, Gung-Won;Jo, Yeong-Cheol;Koh, So-Mi;Kang, Seong-Gook;Kim, Jeong-Mok;Kim, Hae-Seop
    • Food Science and Preservation
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    • v.16 no.5
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    • pp.686-698
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    • 2009
  • We sought basic data for product development and storage improvement of abalone. We explored drying methodologies, such as shade drying, cold air drying, and vacuum freeze drying. We also examined various physicochemical features of both meat and viscera. Raw abalone meat had $78.88{\pm}1.01%$ moisture, $9.24{\pm}0.27%$ crude protein, and $10.05{\pm}0.81%$ carbohydrate (all w/w). The moisture level of dried abalone meat was highest after cold air drying, at $18.38{\pm}0.91%$, and lowest after vacuum freeze drying, at $1.05{\pm}0.05%$. The total amino acid content of raw abalone meat was $17,124.05{\pm}493.18\;mg%$, and fell after shade-drying to $12,969.92{\pm}583.65\;mg%$, and to $13,328.78{\pm}653.11\;mg%$ after cold air drying. The total free amino acid content of raw abalone meat was $4,261.99{\pm}106.55\;mg%$, and rose after shade-drying to $6,336.50{\pm}285.15\;mg%$, to $5,072.04{\pm}248.53\;mg%$ after cold air drying, and to $4,638.85{\pm}218.03\;mg%$ after vacuum freeze drying. The fatty acid proportions in raw abalone meat were $47.00{\pm}0.99%$ saturated, $22.18{\pm}1.05%$ monounsaturated, and $30.82{\pm}1.45%$ polyunsaturated. In the viscera, however, the proportions were $36.72{\pm}0.74%$ saturated, $25.44{\pm}1.12%$ monounsaturated, and $37.84{\pm}1.67%$ polyunsaturated. The contents of chondroitin sulfate in raw abalone were $11.95{\pm}0.35%$ in meat and $7.71{\pm}0.19%$ in viscera (both w/w). After shade-drying, the chondroitin sulfate content was $16.57{\pm}0.90%$ in meat and $9.24{\pm}0.50%$ in viscera. The figures after cold air drying were $16.17{\pm}0.79%$ and $12.44{\pm}0.61%$, and those after vacuum freeze drying $25.17{\pm}1.16%$ and $15.22{\pm}0.70%$ (thus including the highest meat content). The level of collagen in raw abalone was $69.80{\pm}3.07\;mg/g$ in meat and $40.62{\pm}1.79\;mg/g$ in viscera. Meat and viscera dried in the shade had $144.05{\pm}7.78\;mg/g$ and $44.16{\pm}2.39\;mg/g$ collagen, respectively, whereas the figures after cold air drying were $133.29{\pm}6.53\;mg/g$ and $69.20{\pm}3.39\;mg/g$, and after vacuum freeze drying $137.51{\pm}6.33\;mg/g$ and $60.61{\pm}2.79\;mg/g$. Volatile basic nitrogen values of raw abalone showed a higher content in viscera, at $19.01{\pm}0.84\;mg%$, compared to meat ($10.10{\pm}0.44\;mg%$). The value for shade-dried abalone meat was $136.77{\pm}7.37\;mg%$ and that of viscera $197.97{\pm}10.69\;mg%$. After cold air drying the meat and visceral values were $27.32{\pm}1.34\;mg%$ and $71.37{\pm}3.50\;mg%$, respectively.

Egg-forming and Preservation Methods of the Emma Field Cricket Eggs, Teleogrylius emma (Orthoptera: Gryllidae) (왕귀뚜라미(Teleogryiius emma)알의 실내 인공 채란 및 저장)

  • Kim Namjung;Hong Seong-Jin;Seol Kwang-Youl;Kwon Ohseok;Kim Seong-Hyun
    • Korean journal of applied entomology
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    • v.44 no.1 s.138
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    • pp.61-65
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    • 2005
  • Egg-forming and preservation methods for the indoor-rearing system of the emma field cricket, Teleogryllus emma, were investigated. The oviposition preference experiment on different mats, soil(natural oviposition mat) and $oasis^{\circledR}$(artificial oviposition mat), showed that T. emma preferred to oviposit on the $oasis^{\circledR}$. On the other hand, $oasis^{\circledR}$ had more inactive number of eggs laid per female and hatchability than another. However, the number of eggs laid per female and hatchability within 50 days after emergence were better than soil. The experiment on the hatching of the eggs showed that eggs could be stocked at $10^{\circ}C$ for 40 days with 14 day pre-period after laying, representing $62.1\%$ hatchability, when considering generation shortening. On the other hand, When it had been kept in cold storage for 90 days at $7.5^{\circ}C$ with 14 day pre-period after laying it, appeared to be the most suitable for long period-storage method by hatchability $65.1\%$.

Ecological Characteristics and Storage Condition of Diglyphus isaea(Walker) (Hymenoptera: Eulophidae), an Ectoparasite of Liriomyza trifolii(Burgess) (Diptera: Agromyzidae) (아메리카잎굴파리 (Liriomyza trifolii)의 외부기생봉 굴파리좀벌 (Diglyphus isaea)의 생태적 특성 및 저장 조건 구명)

  • Kim, Jeong-Hwan;Byoun, Young-Woong;Kim, Yong-Heon;Kim, Hwang-Yong
    • Korean journal of applied entomology
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    • v.46 no.1 s.145
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    • pp.43-49
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    • 2007
  • Experiments have been performed to investigate biological characteristics of Diglyphus isaea (Walker), an ectoparasite of Liriomyza trifolii (Burgess). At $25^{\circ}C$, development time of D. isaea was estimated as 1.3 days for eggs, 4.5 days for larvae and 6.3 days for pupae. Adult longevity was 25.1 days, and a female adult could lay 305.3 eggs throughout its lifetime. Number of L. trifolii parasitized or killed by the wasp was 962.2 individuals/adult. Most of all parasitized or killed larvae were identified as third instar. With 20% honeyed water as food source, half of the adults could survive 180 days at $5^{\circ}C$, 150 days at $10^{\circ}C$, and 90 days at $15^{\circ}C$. After storing at $10^{\circ}C$ with absolute honey for 30 days, 70 days and 140 days, a female adult in room temperature laid eggs 190.8 individuals, 104.0 individuals and 89.4 individuals, respectively.

Long-term Preservation of Bombyx mori Stocks by Frozen Gonad Storage (생식소 동결에 의한 누에 유전자원의 장기보존)

  • Kim, Sam-Eun;Seong, Su-Il;Lee, Sang-Mong
    • Journal of Sericultural and Entomological Science
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    • v.34 no.1
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    • pp.1-7
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    • 1992
  • For a long-term preservation of silkworm stocks by frozen gonad storage, fundamental topics such as freezing rate and transplanting stage of the gonad, proper cryoprotectant, and super-cooling temperature and freezing point of the freezing medium were examined and following results were obtained. Proper method to anesthetize the ovary-recipient silkworm was to dip the animal to cold water for 10 minutes, and the ovary taken from the 4th instar larvae was more suitable for freezing-preservation than that from the 5th. Concerning the cryoprotectant, glycerol and DMSO were effective to prevent cryoinjury of the ovary, but sorbitol was not. The supercooling temperature and freezing point of the medium to freeze the ovary and testes were checked, and consulting with the results desirable cooling rate was confirmed. On the desirable conditions of transplanting methods, freezing rate and cryoprotectant concentration ect., the next generation was obtained when the females implanted frozen-thawed ovaries mated with normal males, but none of the normal females mated with the males implanted frozen-thawed testes laid fertilized eggs. Now it is needed to improve the connecting ration of the ducts associated with the transplanted testis to those of the hosts.

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