Application of Slow-Freezing Cryopreservation Method for the Conservation of Diverse Potato (Solanum tuberosum L.) Genotypes

  • Zhao Mei-Ai (Department of Plant Biotechnology, Center for the Korea Potato Genetic Resources (KPGR), and Potato Valley Co., Ltd., Kangwon National University) ;
  • Dhital Shambhu P. (Department of Plant Biotechnology, Center for the Korea Potato Genetic Resources (KPGR), and Potato Valley Co., Ltd., Kangwon National University) ;
  • Fang Yi-Lan (Department of Plant Biotechnology, Center for the Korea Potato Genetic Resources (KPGR), and Potato Valley Co., Ltd., Kangwon National University) ;
  • Khu Dong-Man (Department of Plant Biotechnology, Center for the Korea Potato Genetic Resources (KPGR), and Potato Valley Co., Ltd., Kangwon National University) ;
  • Song Ye-Su (Department of Plant Biotechnology, Center for the Korea Potato Genetic Resources (KPGR), and Potato Valley Co., Ltd., Kangwon National University) ;
  • Park Eung-Jun (Department of Plant Biotechnology, Center for the Korea Potato Genetic Resources (KPGR), and Potato Valley Co., Ltd., Kangwon National University) ;
  • Kang Chang-Won (Department of Plant Biotechnology, Center for the Korea Potato Genetic Resources (KPGR), and Potato Valley Co., Ltd., Kangwon National University) ;
  • Lim Hak-Tae (Department of Plant Biotechnology, Center for the Korea Potato Genetic Resources (KPGR), and Potato Valley Co., Ltd., Kangwon National University)
  • Published : 2005.09.01

Abstract

Cryopreservation has been recognized as a practical and efficient tool for the long-term storage of vegetatively propagated plants. This study was conducted to investigate the effects of slow-freezing techniques on the cryopreservation of potato. In vitro plantlets of the potato genotypes of 'Atlantic', 'Superior’, 'Namseo', 'J138', and 'CTO5-5' were cold acclimated, and the excised axillary buds were precultured, osmoprotected, exposed to plant vitrification solution, frozen slowly to $-40^{\circ}C$ and then rapidly plunged into liquid nitrogen, thawed and finally plated on the regeneration medium. It was found that the higher the sucrose concentrations in the subculture medium of donor plantlets, the higher the survival rates of shoot tips after cryopreservation, and the highest survival (20%) was observed in the medium added with 0.25 M sucrose. As for the effect of cooling, $0.3^{\circ}C/min$ cooling speed showed the highest survival (25%). Different varieties showed different responses over different cryopreservation treatments. Survival rate was increased by slow-freezing technique method as compared with that of the basic cryopreservation method of vitrification alone in the diverse potato genotypes. Leaf and tuber morphologies of potatoes regenerated after cryopreservation using slow freezing technique were similar to those derived from the in vitro stock plantlets.

Keywords

References

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