• Journal of Animal Science and Technology
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• 제63권4호
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• pp.919-933
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• 2021

We hypothesized that the unsaturated fatty acid palmitoleic acid (POA) could promote the expression of adipogenic/lipogenic genes in bovine skeletal muscle satellite cells (BSCs). The BSCs were cultured in a growth medium containing 10% fetal bovine serum. When the cells reached 80%-90% confluence, we used the differentiation medium with 5% horse serum for differentiation for 96 h. The differentiation medium contained 50 µM, 100 µM and 200 µM POA. Control BSC were cultured only in differentiation media. Compared with the control BSC, the POA BSC significantly up-regulated the expression of paired box 3 (Pax3) and paired box 7 (Pax7) and down-regulated myogenin gene expression (p < 0.01), which indicates a depression in muscle fiber development. However, all POA treatments up-regulated the expression of the adipocyte transcription factors peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein alpha and beta (C/EBP α and C/EBP β), and other genes (p < 0.01) and increased the expression of PAT-family proteins and the concentration of adiponectin in the media. These results indicate that POA can convert part of BSCs into adipocytes.

• 한국해양공학회지
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• 제11권3호
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• pp.8-19
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• 1997

In this study an adequate type of offshore structure at the Sakhalin region as cold region is proposed and its structural design results are presented based on the reliability analysis. Structural safety assessment has been carried out for the proposed offshore structure at the Sakhalin area as designed by the reliability method. And a rational design procedure is presented based on the reliability analysis. Followings are drawn through the present study : - Four colum TLP structure is proposed as an adequate offshore structure type at the cold region like the Sakhalin region and the reliability-based structural design results are presented. It is seen that the proposed type is a more adequate and economic than the fixed type. - Safety assessment of the proposed structure applying the extended incremental load method is performed. - Referring the allowable safety level for offshore structures it has been found present TLP structure has sufficient structural safety at the system level as well as at the component level.

• 열처리공학회지
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• 제27권2호
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• pp.59-64
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• 2014

Cu-Al-Mn shape memory alloys of a variety of composition were characterized in terms of shape memory properties and cold workability. Cold workability tested by cold rolling indicated that the alloys solution treated in the ${\alpha}+{\beta}$ region have a higher ductility than those solution treated in the ${\beta}$ region. Also it is known that cold workability increased with the decrease in Al content in the ${\beta}$ region. This seems to be resulted from the fact that Mn addition causes to expand ${\beta}$ region toward lower Al content and lower order-disorder transition temperature, consequently, ${\beta}$ of excellent workability being frozen even at room temperature. Experimental results regarding shape memory showed that the properties were better with a higher Al contents at a given Mn content, which is closely related with martensitic transformation. It is also shown that super elasticity limit was enhanced with decrease in the yield strength of alloys because a lower yield strength seems to initiates slip at the lower applied stress.

• Journal of Animal Science and Technology
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• 제63권4호
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• pp.934-953
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• 2021

Ciglitazone is a member of the thiazolidinedione family, and specifically binds to peroxisome proliferator-activated receptor-γ (PPARγ), thereby promoting adipocyte differentiation. We hypothesized that ciglitazone as a PPARγ ligand in the absence of an adipocyte differentiation cocktail would increase adiponectin and adipogenic gene expression in bovine satellite cells (BSC). Muscle-derived BSCs were isolated from six, 18-month-old Yanbian Yellow Cattle. The BSC were cultured for 96 h in differentiation medium containing 5 µM ciglitazone (CL), 10 µM ciglitazone (CM), or 20 µM ciglitazone (CH). Control (CON) BSC were cultured only in a differentiation medium (containing 2% horse serum). The presence of myogenin, desmin, and paired box 7 (Pax7) proteins was confirmed in the BSC by immunofluorescence staining. The CL, CM, and CH treatments produced higher concentrations of triacylglycerol and lipid droplet accumulation in myotubes than those of the CON treatment. Ciglitazone treatments significantly increased the relative expression of PPARγ, CCAAT/enhancer-binding protein alpha (C/EBPα), C/EBPβ, fatty acid synthase, stearoyl-CoA desaturase, and perilipin 2. Ciglitazone treatments increased gene expression of Pax3 and Pax7 and decreased expression of myogenic differentiation-1, myogenin, myogenic regulatory factor-5, and myogenin-4 (p < 0.01). Adiponectin concentration caused by ciglitazone treatments was significantly greater than CON (p < 0.01). RNA sequencing showed that 281 differentially expressed genes (DEGs) were found in the treatments of ciglitazone. DEGs gene ontology (GO) analysis showed that the top 10 GO enrichment significantly changed the biological processes such as protein trimerization, negative regulation of cell proliferation, adipocytes differentiation, and cellular response to external stimulus. Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that DEGs were involved in the p53 signaling pathway, PPAR signaling pathway, biosynthesis of amino acids, tumor necrosis factor signaling pathway, non-alcoholic fatty liver disease, PI3K-Akt signaling pathway, and Wnt signaling pathway. These results indicate that ciglitazone acts as PPARγ agonist, effectively increases the adiponectin concentration and adipogenic gene expression, and stimulates the conversion of BSC to adipocyte-like cells in the absence of adipocyte differentiation cocktail.

• Journal of Astronomy and Space Sciences
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• 제37권2호
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• pp.95-104
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• 2020

Properties of plasmas that constitute the plasma sheet in the near-Earth magnetotail vary according to the solar wind conditions and location in the tail. In this case study, we present multi-spacecraft observations by Cluster that show a transition of plasma sheet from cold, dense to hot, tenuous state. The transition was associated with the passage of a spatial boundary that separates the plasma sheet into two regions with cold, dense and hot, tenuous plasmas. Ion phase space distributions show that the cold, dense ions have a Kappa distribution while the hot, tenuous ions have a Maxwellian distribution, implying that they have different origins or are produced by different thermalization processes. The transition boundary separated the plasma sheet in the dawn-dusk direction, and slowly moved toward the dawn flank. The hot, tenuous plasmas filled the central region while the cold, dense plasmas filled the outer region. The hot, tenuous plasmas were moving toward the Earth, pushing the cold, dense plasmas toward the flank. Different types of dynamical processes can be generated in each region, which can affect the development of geomagnetic activities.

• 한국군사과학기술학회지
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• 제12권4호
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• pp.415-423
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• 2009

To investigate low frequency acoustic transmissions in the Sub-Polar Front(SPF) of the East Sea, numerical experiments are conducted with Range dependent Acoustic Model(RAM) using Circulation Research of the East Asian Marginal Seas(CREAMS) data and Autonomous Profiling Explorer(APEX)) data. Significant seasonal variations of sea water properties are existed across the Sub-Polar Front(SPF) region from the north and the south. The model results show that Transmission Loss(TL) decrease(about 20dB) with ideal front in the warm region whereas TL increase(about 25dB) with ideal front in the cold region. Regardless of season(both in summer and winter), when the sound source is located in the cold region of the SPF, the model results show weak TL, compared to the case of the source in the warm region(Maximum difference of TL reaches 28dB). This difference between the cases when the source is located in the cold region and the warm region, is accounted for from the different vertical profiles of sound speed in both regions.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2002년도 Proceedings of International Symposium on Remote Sensing
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• pp.655-655
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• 2002

Spatial distribution of phytoplankton pigment concentration (PPC) and sea surface temperature (SST) around the East Korean Warm Current (EKWC) was described, using both ocean color images and advanced very high resolution radiometer (AVHRR) images. Water mass in this region can be classified into five categories in the horizontal profile of PPC and SST, nLw(normalized water-leaving radiance) images: (1) coastal cold water region associated with concentrations of dissolved organic material or yellow colored substances and suspended sediments, (2) cold water region of thermal frontal occurred by a combination of phytoplankton absorption and suspended materials, (3) warm water overlay region by the phytoplankton absorption than the suspended materials; (4) warm water region occurred by the low phytoplankton absorption, and (5) offshore region occurred by the high phytoplankton absorption. In particular, the highest PPC area appeared in the ocean color and SST images with a band shaped distribution of the thermal front and ocean color front region, which is located the coastal cold waters along western thermal front of the warm streamer of the EKWC.

• 한국건축시공학회:학술대회논문집
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• 한국건축시공학회 2016년도 추계 학술논문 발표대회
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• pp.89-90
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• 2016

In the cold-weathering(Mongolian region) concrete construction, this purpose of this research is the evaluation on the performance of concrete using various binders such as Mongolian cement, Chinese cement and high blane-self heated cement.

• 대한물리치료과학회지
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• 제2권3호
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• pp.577-586
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• 1995

This paper was the study of a change in the body temperature of each region(Oral cavity, Axillary, Cubital, Popliteal, Inguinal region)and by time after appling hot(Hot Pack, Infra Red) and cold (Ice Pack, Cooling Pack) to lumbar region. The statistical measures were sampled through 16 senior students in dept. of Physiothrapy, Kim Cheun College and the period for this was from July 13, 1995 to July 20, 1995. There was an increse in the temperature of distal portion besides application area after hot application. The increse in the body temperature according to applied agents and time showed significance statistically(p<0.01, p<0.05). There was just a decrease in the temperature of distal portions besides application area after appling Ice Pack. Furthermore there was an increase in the body temperature after appling Cooling Towel. Therefore, during cold application the appling agents and time did not showed any significance statistically.

• Journal of Welding and Joining
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• 제12권2호
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• pp.87-96
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• 1994

Correlation between microstructural features and segregation of elements (Si, Mn, P and S) near the mid of thickness in the base metal and the synthetic HAZ was investigated. Furthermore, the relationship between the degree of center segregation and weld cold cracking susceptibility in the thickness direction was also conducted by evaluating the effect of P concentration on the critical applied stress. The results obtained are as follows: 1) Pearlite band, containing the MnS type inclusion and a locally transformed structure with a higher hardness, was observed in the center segregation region. 2) By the weld thermal cycle, center segregation region was transformed to the white band which had a higher hardness than that of base metal due to a greater hardenability of concentrated Mn, P etc.. 3) Weld cold cracking susceptibility in the thickness direction was mainly dependent on the concentration of impurity elements rather than on the number of the segregated particles near the mid of thickness. 4) During welding, the higher concentrated region was easily changed into white band. Therefore, it could be predicted that the initiation and propagation of a cold crack would be promoted by increasing the restraint stress and hydrogen content.