• Journal of Forest and Environmental Science
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• 제38권4호
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• pp.284-289
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• 2022

This study was conducted to determine the seed dormancy types of Sambucus racemosa subsp. pendula Nakai and S. williamsii Hance. Low temperature stratification (1, 2, 3, 4 months) and GA3 treatment (1,000 mg/L) were performed on seeds to determine the type of seed dormancy. After the treatment, seeds were placed on a petri dish at 25℃ under light conditions. The germination rate and mean germination time were investigated. Results showed that cold stratification was effective in breaking the dormant state of the seed in both species. In the low temperature stratification treatment, the seed germination performance was improved as the treatment period was prolonged. Gibberellin treatment was effective in breaking the dormant state of S. racemosa subsp. pendula without low temperature stratification. However, S. williamsii did not break the dormant state of the seed by gibberellin treatment without low temperature stratification treatment. In the gibberellin treatment, germination performance was improved according to the low temperature stratification treatment period. As a result of this study, the seeds of S. williamsii have both an intermediate complex and a deep complex morphophysiological dormancy (MPD). In comparison, it was found that the S. racemosa subsp. pendula had intermediate composite MPD.

• 원예과학기술지
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• 제29권5호
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• pp.488-493
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• 2011

Callus culture initiation of strawberry (Fragaria${\times}$ananassa Duch.) was investigated at different Murashige and Skoog (MS) medium strengths, types and concentrations of plant growth regulators, and incorporating a cold pretreatment period to determine the optimal nutritional and environmental conditions. No high quality callus was induced on MS media without auxin regardless of medium strength. When 6-benzylaminopurine (BA) was combined with indole acetic acid (IAA), naphthalene acetic acid (NAA), and 2,4-dichlorophenoxyacetic acid (2,4-D), high quality callus were highly induced compared to medium supplemented with auxin alone. When $0.5mg{\cdot}L^{-1}$ BA was combined with IAA, NAA, and 2,4-D, high quality callus induction was more effective than the medium supplemented with the other BA concentrations. The best combination of auxin and cytokinin for high quality callus induction was $1.0mg{\cdot}L^{-1}$ NAA and $0.5mg{\cdot}L^{-1}$ BA. Although the differences in callus induction were not significant, high quality callus induction at half strength MS medium was more effective than at full strength medium. When $30g{\cdot}L^{-1}$ sucrose was added to the half strength MS medium, the rate of high quality callus induction increased. The optimum cold pretreatment temperature and period for high quality callus induction were $4^{\circ}C$ and 72 h, respectively. Regeneration rate of high quality callus increased in MS medium supplemented with thidiazuron.

• 한국제4기학회지
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• 제17권2호
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• pp.55-61
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• 2003

In the year 2000 we culminated a successful five year investigation of climate change by completing a preliminary east-west transect across Mongolia. An earlier tree-ring study at Tarvagatay Pass, Mongolia indicated unusual warming during the 20th century similar to other paleo-investigations of the northern hemisphere. This record had represented one of the few tree-ring records for central Asia. New data from several sites in western Mongolia confirmed the preliminary temperature. The highest twenty-year growth period for the composite record is from 1973-1994. The western Mongolian record was significantly correlated with the Taimyr Peninsula and two northern hemisphere temperature reconstructions reflecting large-scale temperature patterns while showing some important regional differences. These differences should prove useful for climate models. We have also developed a millennial length temperature-sensitive record at the Solongotyin Davaa site (formerly Tarvagatay Pass) using relict wood and living trees. Conspicuous features over the last 1000 years are a century scale temperature decline punctuated by the end of the Little Ice Age in the late-1800s and 20th century warming. The record also shows a cold period early in the 12th century and warm intervals late in the 10th, early in the 15th and at end of the 18th centuries. Despite a limited sample size before 900 AD, the long Solongotyin Davaa record is useful in indicating severe cold events and suggests some cold intervals nearly as severe. These tree ring series, spanning much of the circumpolar northern treeline, have been compiled to create a long-term reconstruction of the Earth's temperature over centuries. The new chronology, in addition to its value as a detailed record of Mongolian climate, provides independent corroboration for such hemispheric and global reconstructions and their indications of unusual warming during the 20th century.

• 대한기계학회논문집B
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• 제24권8호
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• pp.1038-1045
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• 2000

An experimental study was carried out to delineate the flow characteristics in a closed countescurrent two-phase thermo syphon with concentric tubes. This is to be installed in the HANARO research reactor as a part of a Cold Neutron Source(CNS). In the present investigation, experiments ata room temperature with Freon-II3 as a moderator were performed. Results show that, based on the magnitude of pressure fluctuation, the flow regimes could be divided into 4 distinct ones in the ($V_f,\;Q_i$) plane, where $V_f$ represents the volume of the charged liquid and $Q_i$ the heat load: a stable flow regime, an oscillatory flow regime, a restablized flow regime and a dryout flow regime. For $V_f$>2.5l, the flow is stable at low $Q_i$. However, as $Q_i$ increases, the flow becomes oscillatory and finally restablizes As $V_f$ increases, the oscillation amplitude decreases, reaching to the restablized flow region at low $Q_i$, and the liquid level in the moderator cell remains high. In the oscillatory flow regimes, for a fixed VI; the oscillating period of time varies with $Q_i$, having a minimum value at a certain value of $Q_i$. The heat load, where the oscillating period of time is minimum, decreases as $V_f$ increases.

• The Korean Journal of Physiology and Pharmacology
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• 제12권6호
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• pp.299-306
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• 2008

Sometimes, spinal cord injury (SCI) results in various chronic neuropathic pain syndromes that occur diffusely below the level of the injury. It has been reported that behavioral signs of neuropathic pain are expressed in the animal models of contusive SCI. However, the observation period is relatively short considering the natural course of pain in human SCI patients. Therefore, this study was undertaken to examine the time course of mechanical and cold allodynia in the hindpaw after a spinal cord contusion in rats for a long period of time (30 weeks). The hindpaw withdrawal threshold to mechanical stimulation was applied to the plantar surface of the hindpaw, and the withdrawal frequency to the application of acetone was measured before and after a spinal contusion. The spinal cord contusion was produced by dropping a 10 g weight from a 6.25 and 12.5 mm height using a NYU impactor. After the injury, rats showed a decreased withdrawal threshold to von Frey stimulation, indicating the development of mechanical allodynia which persisted for 30 weeks. The withdrawal threshold between the two experimental groups was similar. The response frequencies to acetone increased after the SCI, but they were developed slowly. Cold allodynia persisted for 30 weeks in 12.5 mm group. The sham animals did not show any significant behavioral changes. These results provide behavioral evidence to indicate that the below-level pain was well developed and maintained in the contusion model for a long time, suggesting a model suitable for pain research, especially in the late stage of SCI or for long term effects of analgesic intervention.

• 한국식품저장유통학회지
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• 제12권1호
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• pp.1-7
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• 2005

저온$(5^{\circ}C)$에서 가루차를 일반포장, 일반포장+항산화제, 일반포장+방습제처리시 4개월 이후 품질관련 성분인 총질소, 총아미노산, 탄닌, 카페인, 엽록소, 지방산 함량이 상당히 저하되었으며, 일반포장 + 진공처리는 일반포장 저장에 비해 1개월 정도 저장기간의 연장이 가능하였다. 제품의 표면색상은 저온$(5^{\circ}C)$보관시 일반포장에서는 저장 1개월에 a값(녹색)이 -16.43에서 저장 5개월에는 $42\%$정도 감소된 -10.11로 크게 감소되었다. 외관과 내질에 따른 관능평가시 저온$(5^{\circ}C)$저장의 경우 일반포장 내에서 1개월 경과시 89점이었으나 3개월 보관시 78점으로 가루차의 외관과 내질이 크게 열악해졌으며, 일반포장 + 진공의 경우 4개월 보관시 79점으로 일반포장보다 1개월 정도 더 연장보관이 가능하였다. 본 연구에서는 진공포장한 가루차를 $5^{\circ}C$에서 저장한 것이 처리구 중 가장 우수한 결과를 나타내었다.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 심포지엄
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• pp.9-18
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• 2003

A method for rapid propagation of mature Jack fruit was developed. Four types of explants (mature embryos, apical meristems of young seedlings, apices from mature plants and nodal segments) were used. It has been found 88% of young apical meristems produced shoots in Campbell and Durzan (CD) medium compared to 60% in Murashige and Skoog (MS) medium. Only 1/3 of them produced multiple shoots. Shoot idtiation from nodal segments was very rare. Mature apices produced callus. Although removed of the sheathing cover around mature buds enhanced the shoot initiation but success rate was low in growth regulator free medium. Embryos respond to the CD medium but not to the MS medium. Embryos from seeds soaked in water for 24 hours produced shoots after 8 weeks of incubation and the success rate was 70% while embryos from dry seeds only produced roots. There was no significant effect of cold storage (refrigeration) for 7 days on shoot initiation from mature embryos (65%) but the ability for shoot induction declines with storage time (55% after 21 days of cold storage). Mature axillary buds were established in Modified Campbell and Durzan (CD) medium supplemented with 0.5mg/1 and IBA. There was a significant difference in the growth performance of shoots according to the period of the year in which explants were collected. Highest (60%) was observed in November-January period. It was only 30% when the explants were collected in February-April or May-July and decreased to 20% in August-October. The shoots produced in November-January showed a higher vigor than those produced in other months. Since Jak fruit show seasonal changes in fruit bearing and shedding of leaves, it can be suggested that the difference in growth performances of tissues cultured in artificial culture media would have been affected by endogenous rhythms.

• 한국환경과학회지
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• 제24권7호
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• pp.851-864
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• 2015

This study analyzed the climate regime shift using statistical change-point analysis on the time-series tropical cyclone (TC) frequency that affected Japan in July to September. The result showed that there was a significant change in 1995 and since then, it showed a trend of rapidly decreasing frequency. To determine the reason for this, differences between 1995 to 2012 (9512) period and 1978 to 1994 (7894) period were analayzed. First, regarding TC genesis, TCs during the 9512 period showed a characteristic of genesis from the southeast quadrant of the tropical and subtropical western North Pacific and TCs during the 7894 period showed their genesis from the northwest quadrant. Regarding a TC track, TCs in the 7894 period had a strong trend of moving from the far east sea of the Philippines via the East China Sea to the mid-latitude region in East Asia while TCs in the 9512 period showed a trend of moving from the Philippines toward the southern part of China westward. Thus, TC intensity in the 7894 period, which can absorb sufficient energy from the sea as they moved a long distance over the sea, was stronger than that of 9512. Large-scale environments were analyzed to determine the cause of such difference in TC activity occurred between two periods. During the 9512 period, anomalous cold and dry anticyclones were developed strongly in the East Asia continent. As a result, Korea and Japan were affected by the anomalous northerlies thereby preventing TCs in this period from moving toward the mid-latitude region in East Asia. Instead, anomalous easterlies (anomalous trade wind) were developed in the tropical western Pacific so that a high passage frequency from the Philippine to the south China region along the anomalous steering flows was revealed. The characteristics of the anomalous cold and dry anticyclone developed in the East Asia continent were also confirmed by the analysis of air temperature, relative humidity and sensible heat net flux showing that most regions in East Asia had negative values.

• Journal of Acupuncture Research
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• 제29권5호
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• pp.97-108
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• 2012

Objectives : To demonstrate the importance of syndrome differentiation in clinical research of herb medication, through the comparative study on efficacy and safety of herb medication according to cold-heat tendency of OA knee patients. Methods : During December 2010 to July 2011, 138 knee OA patients were randomly assigned to WIN-34B 600mg(300mg, b.i.d.), 1,200mg(600mg, b.i.d.) and placebo b.i.d. for 8 weeks. Patient were re-classified into cold-heat tendency group according to cold-heat questionnaires. To investigate efficacy and safety, we assessed the 100mm pain VAS at baseline and 8 weeks later, and we monitored adverse event of patients during treatment period. Results : 1. Efficacy study : In WIN-34B 1,200mg group, VAS mean changes of heat tendency group showed slightly increase than those of cold tendency, but no significant difference within two groups. In heat tendency group, WIN-34B 1,200mg group showed a significant decrease of VAS compared to placebo group. but there were no significant difference in cold tendency group. 2. Safety study; In WIN-34B 600mg group, incidence of adverse events of cold tendency group was higher than those of heat tendency, but not in WIN-34B 1,200mg group. Conclusions : This study suggests that WIN-34B tend to have more efficacy in heat tendency-knee OA patients and WIN-34B is safe drug relatively, regardless of cold-heat tendency. In further clinical research on efficacy and safety of WIN-34B, stratification using syndrome differentiation is required.

• 해양환경안전학회지
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• 제27권6호
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• pp.701-707
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• 2021

본 연구에서는 강한 한파가 발생했던 2018년과 온난 한파가 발생했던 2019년의 기온에 따른 수온의 반응 및 지연시간과 북풍계열 바람 빈도와의 상관관계를 분석하였다. 사용된 시간 자료는 국립수산과학원에서 제공하는 7개 지점 해역별 수온자료와 수온관측소 인근 7개 지점 AWS 기온자료를 이용하였다. 관측되지 못한 자료는 내삽법으로 근사값을 계산하였고, FIR Filter를 이용하여 자료의 주기성을 파악하였다. 그 결과, 강한 한파가 발생했던 2018년은 북풍계열 바람을 통해 차가운 공기가 남하하면서 기온을 하강시켜 전 해역에 저수온을 유발한 반면 온난 한파가 발생했던 2019년은 평년 수준의 기온으로 하강하였지만 수온은 크게 변화하지 않았다. 강한 한파가 발생했던 2018년 기온 하강에 따른 수온의 지연시간은 평균 14시간으로 0.7 이상의 높은 상관성을 나타냈고 온난 한파가 발생했던 2019년은 평균 지연시간이 20시간으로 0.44-0.67 사이의 상관성을 보였다. 본 연구를 통해 해역별로 기온 하강에 따른 표층수온의 반응을 해석하였고 지연시간을 파악함으로써 양식생물의 피해를 최소화하고 한파 피해의 신속한 대응에 기여할 수 것으로 기대한다.