• Journal of Plant Biotechnology
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• v.49 no.2
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• pp.118-123
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• 2022

To obtain information on the molecular mechanism underlying the low temperature tolerance of sweet potato [Ipomoea batatas (L.) Lam], the proteome expressed in the sweet potato cultivar Xushu 15-1 with high cold storage tolerance and in the cultivar Xushu 15-4 with low cold storage tolerance was analyzed using 2-D and MALDI-TOF/TOF analyses. Compared with the control (without cold treatment), four protein spots were newly expressed in Xushu 15-1. The expression level of one protein spot was higher in Xushu 15-4 than in Xushu 15-1. Spot 2, which was newly expressed in Xushu 15-1, was identified as sporamin. Assessment of the change in protein expression levels over 8 weeks in the storage roots of the two cultivars treated at 4℃ revealed no significant difference in the expression levels in Xushu 15-1 over time. However, in Xushu 15-4, the expression level of one protein spot increased, while those of four spots decreased. Of the proteins with reduced expression levels, spots 7 and 8 were identified as actin and spots 9 and 10 were identified as fructokinase-like proteins. The present results are expected to enhance the understanding of the complex mechanism underlying the low temperature tolerance of sweet potatoes during storage and can be used to identify candidate genes for the development of new varieties of sweet potatoes with improved low temperature tolerance during cold storage in the future.

• International Journal of Industrial Entomology and Biomaterials
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• v.9 no.1
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• pp.117-121
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• 2004

The patterns of diapause-associated proteins of silkworm eggs were analyzed by two-dimensional (2-D) gel electrophoresis. Among the hundreds of spots on the 2-D gels, at least two proteins were considered to be associated with diapause. A protein, spot 4, with an approximate molecular weight of 38 kDa and pI 6.1 was observed in the HCI-treated, cold-treated, and diapause eggs, respectively. Spot 4 was undetectable in unfertilized eggs and non-diapause eggs at two days after oviposition, suggesting that this protein may be associated with the entrance to diapause. A protein, spot 11, with an approximate molecular weight of 21 kDa and pI of 61 was detected in the unfertilized, HCl-treated, and cold-treated eggs, respectively, after oviposition by normal moths. In diapausing eggs, a protein corresponding to spot 11 was observed in 3-, 5-, and 30-day-old eggs, while the protein was not detected one-day-old eggs. The protein corresponding to spot 11 was not detected in unfertilized and non-diapause eggs obtained from subesophabeal ganglion (SG)-extirpated moths either. Spot 11 was also considered to be a diapause specific protein, which occurred at only early embryonic stage under the control of diapause-downregulated gene.

• Journal of Microbiology and Biotechnology
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• v.15 no.4
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• pp.831-837
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• 2005

Low-temperature adaptation and cryoprotection were studied in Leuconostoc mesenteroides SYl, a strain isolated from Kimchi. L. mesenteroides SY1 cells grown in exponential growth phase at $30^{\circ}C$ were exposed to $15^{\circ}C,\;10^{\circ}C$, and $5^{\circ}C$ for 2, 4, and 6 h, respectively, and then frozen at $- 70^{\circ}C$ for 24 h. Survival ratio was measured after the cells were thawed. The freezing-thawing cycles were repeated four times. Preadapted cells survived better than non-adapted control cells, and the highest survival ratio ($96\%$) was observed for cells preadapted for 2 h at $5^{\circ}C$, whereas control cells showed only $22\%$. The 2D gel showed that two proteins (spots A and B) were induced in cells preadapted at lower temperatures. Spots A and B have the same molecular weight (7 kDa), but the pI was 4.6 for spot A and 4.3 for spot B. The first 29 and 15 amino acid sequences from spots A and B were determined, and they were identical, except for one amino acid. A csp gene was cloned, and nucleotide sequencing confirmed that the gene encoded spot A cold shock protein.

• Journal of Power System Engineering
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• v.5 no.1
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• pp.80-88
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• 2001

The spot welding method has been used in the joining of structures, automotive body, railway carriage, aircraft, household electric appliances, precision parts etc., because of brief working, easy automation, available mass production, and convenience. In this paper, the effects of welding conditions on the fatigue life and the prediction of fatigue life based on fracture mechanics theory of spot welded joint were investigated. Fatigue tests were conducted with the tensile-shear specimens welded in the various current using cold rolled steel sheets. Fatigue life of spot welded joint was predicted and compared with experimental results. Using FEM(finite element method), we analysed the distribution of stress and the condition of deformation on the environments of nugget.

• Journal of the Korean Society of Manufacturing Technology Engineers
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• v.21 no.2
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• pp.290-296
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• 2012

Cold-rolled carbon steel sheets are commonly used in railroad car or commercial vehicles such as the automobile. These are mainly fabricated by spot welding which is a kind of electric resistance welding. But fatigue strength of spot welding joint is lower than that of base metal due to high stress concentration at nugget edge of the spot welded part. And fatigue strength of them is especially influenced by not only geometrical and mechanical factors but also welding conditions of the spot welded joint. So for fatigue design of gas welded joints such as TS-type joints, it is necessary to obtain design information on stress distribution at the weldment as well as fatigue strength of spot welded joints. And also, the influence of the geometrical parameters of spot welded joints on stress distribution and fatigue strength must be evaluated. And analysis approach for fatigue test using design of experiment are evaluated optimum factor in TS-type welded joint and geometrical parameters of materials. Using these results, that factors applied to fundamental information for automation of fatigue design.

• Journal of The Korean Society of Grassland and Forage Science
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• v.25 no.4
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• pp.287-296
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• 2005

To investigation protein expression pattern in rice leaves exposed to cold stress, the soluble proteins extracted from leaf tissue were fractionated with $15\%$ PEG and separated by two-dimensional polyacrylamide gel electrophoresis (2-DE). Differentially expressed proteins were identified by peptide mass fingerprinting using matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Eight proteins up-regulated and 10 down-regulated were found in $15\%$ PEG supernatant fraction. In addition, 13 proteins up-regulated and 14 down-regulated were found in $15\%$ PEG pellet fraction. It was identified the differentially expressed proteins in $15\%$ PEG supernatant fraction as pimerase/dehydratase fructokinase, ribose-5-phosphate isomerase (Rpi), chaperonin 21 precursor, probable photosystem II oxygen-envolving complex (PS II OEC) protein 2 precursor and thioredoxin h-type (Trx-h) and those in $15\%$ PEG pellet fraction as OSINBb0059K02.15, hypothetical protein, putative mitogen-activated protein kinase kinase (MAPKK), beta 7 subunit of 205 proteasome, ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) small subunit. These proteins are involved in metabolism, energy, protein synthesis, disease/defense and signal transduction-related proteins.

• Proceedings of the KWS Conference
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• 2007.11a
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• pp.342-344
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• 2007

The resistance spot weldability of surface textured cold-rolled steel sheet was evaluated. One steel sheet(T4.5) showed reduced electrode life with less than 2000 welds, and all other steel sheets(E2.2, E4.5, T2.2) made more than 2500 welds. The carbon imprint test revealed that there is sudden electrode diameter increase around 1700 welds. It is believed that the increased electrode diameter decreased current density, and resulted in decreasing weld electrode life due to small weld button size. It is considered that surface roughness difference may attribute to heating during weld cycle and reduced the weld electrode life.

• Journal of Welding and Joining
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• v.17 no.4
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• pp.69-75
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• 1999

Spot welding has been used in the sheet metal joining processes because of its high productivity and convenience. In this study, predicting methods of fatigue life of spot welded joint have been investigated and fatigue and static tests were conducted with the peel-tension specimens using cold rolled steel plate(SPCC). Fatigue life of peel-tension spot welded joint was influenced by tail effect. Fatigue life evaluation using modified stress index parameter, considering the effective eccentric length, can predict the life more exactly than conventional stress index parameter.

• Proceedings of the KWS Conference
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• 2007.11a
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• pp.199-202
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• 2007

The center floor is a part of underbody of cars. The assembling method is mostly a resistant spot welding. But the resistant spot welding has many demerits in productivity such as low welding speed, large spatter occurring, hard to verify the welding quality. The laser welding has many merits such as high welding speed, high static and dynamic weld strength. So the laser welding has been studied to substituted the resistant spot welding. Therefore, in this study, as a pre-evaluation stage for replacing the resistance spot welding with laser in production of automobile parts, the weldability of cold rolled and zinc coated steel sheets is studied and also evaluated the weld strength by the tensile shear test. And the shape of weld bead appearances and micro structure of the weld bead section is also evaluated.

• 한국기계연구소 소보
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• s.9
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• pp.75-82
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• 1982