Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Proteome analysis of storage roots of two sweet potato cultivars with contrasting low temperature tolerance during storage

저온 저장 감수성 및 저항성 고구마 품종에서 저온 반응성 단백질체 연구

  • Kim, Yun-Hee (Department of Biology Education, College of Education, IALS, Gyeongsang National University) ;
  • Ji, Chang Yoon (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Ho Soo (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Chung, Jung-Sung (Department of Agronomy, College of Agriculture and Life Sciences, Gyeongsang National University, IALS) ;
  • Choi, Sung Hwan (Division of Horticulture Science, College of Agriculture and Life Sciences, Gyeongsang National University, IALS) ;
  • Kwak, Sang-Soo (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Lee, Jeung Joo (Department of Plant Medicine, College of Agriculture and Life Sciences, IALS, Gyeongsang National University)
  • 김윤희 (경상국립대학교 사범대학 생물교육과(농업생명과학연구원)) ;
  • 지창윤 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 김호수 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 정정성 (경상국립대학교 농업생명과학대학 농학과(농업생명과학연구원)) ;
  • 최성환 (경상국립대학교 농업생명과학대학 원예과학부(농업생명과학연구원)) ;
  • 곽상수 (한국생명공학연구원 식물시스템공학연구센터) ;
  • 이증주 (경상국립대학교 농업생명과학대학 식물의학과(농업생명과학연구원))
  • Received : 2022.03.30
  • Accepted : 2022.04.29
  • Published : 2022.06.30
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Abstract

To obtain information on the molecular mechanism underlying the low temperature tolerance of sweet potato [Ipomoea batatas (L.) Lam], the proteome expressed in the sweet potato cultivar Xushu 15-1 with high cold storage tolerance and in the cultivar Xushu 15-4 with low cold storage tolerance was analyzed using 2-D and MALDI-TOF/TOF analyses. Compared with the control (without cold treatment), four protein spots were newly expressed in Xushu 15-1. The expression level of one protein spot was higher in Xushu 15-4 than in Xushu 15-1. Spot 2, which was newly expressed in Xushu 15-1, was identified as sporamin. Assessment of the change in protein expression levels over 8 weeks in the storage roots of the two cultivars treated at 4℃ revealed no significant difference in the expression levels in Xushu 15-1 over time. However, in Xushu 15-4, the expression level of one protein spot increased, while those of four spots decreased. Of the proteins with reduced expression levels, spots 7 and 8 were identified as actin and spots 9 and 10 were identified as fructokinase-like proteins. The present results are expected to enhance the understanding of the complex mechanism underlying the low temperature tolerance of sweet potatoes during storage and can be used to identify candidate genes for the development of new varieties of sweet potatoes with improved low temperature tolerance during cold storage in the future.

고구마의 저온 내성 반응의 분자적 기작에 대한 정보를 얻기 위해 저온 저장 내성이 높은 고구마 품종 Xushu 15-1과 저온 저장 내성이 낮은 Xushu 15-4에서 발현되는 단백질체를 2-D와 MALDI-TOF/TOF를 이용하여 분석하였다. 저온 처리가 되지 않은 대조구 간의 분석 결과, Xushu 15-1에서는 4개의 단백질 spot이 새롭게 발현되었으며, Xushu 15-4에서는 1개 단백질 spot의 발현량이 Xushu 15-1에 비하여 더 높았다. 이들 중 Xushu 15-1에서 새롭게 발현된 spot 2는 sporamin으로 동정되었다. 4℃ 처리된 2개 품종의 저장뿌리에서 8주 동안의 단백질 발현량의 변화를 조사한 결과, Xushu 15-1에서는 별다른 차이가 없었다. 그러나, Xushu 15-4에서는 1개 단백질 spot의 발현량이 증가하였고 4개 spot들의 발현량은 감소하였다. 발현량이 감소된 단백질 중 spot 7 및 8번은 actin, spot 9 및 10번은 fructokinase-like protein으로 동정되었다. 본 연구의 결과는 저장 중 고구마의 저온 내성과 관련된 복잡한 반응성 기작에 대한 이해를 높이고, 향후 저온 저장 능력이 향상된 신품종 고구마의 개발을 위한 후보 유전자를 특정하는 데 이용될 수 있을 것으로 사료된다.

Keywords

Acknowledgement

본 연구는 한국연구재단 중견연구자지원사업(2021R1A2C400188711)의 지원으로 수행되었다.

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