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http://dx.doi.org/10.5010/JPB.2022.49.2.118

Proteome analysis of storage roots of two sweet potato cultivars with contrasting low temperature tolerance during storage  

Kim, Yun-Hee (Department of Biology Education, College of Education, IALS, Gyeongsang National University)
Ji, Chang Yoon (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology)
Kim, Ho Soo (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology)
Chung, Jung-Sung (Department of Agronomy, College of Agriculture and Life Sciences, Gyeongsang National University, IALS)
Choi, Sung Hwan (Division of Horticulture Science, College of Agriculture and Life Sciences, Gyeongsang National University, IALS)
Kwak, Sang-Soo (Plant Systems Engineering Research Center, Korea Research Institute of Bioscience and Biotechnology)
Lee, Jeung Joo (Department of Plant Medicine, College of Agriculture and Life Sciences, IALS, Gyeongsang National University)
Publication Information
Journal of Plant Biotechnology / v.49, no.2, 2022 , pp. 118-123 More about this Journal
Abstract
To obtain information on the molecular mechanism underlying the low temperature tolerance of sweet potato [Ipomoea batatas (L.) Lam], the proteome expressed in the sweet potato cultivar Xushu 15-1 with high cold storage tolerance and in the cultivar Xushu 15-4 with low cold storage tolerance was analyzed using 2-D and MALDI-TOF/TOF analyses. Compared with the control (without cold treatment), four protein spots were newly expressed in Xushu 15-1. The expression level of one protein spot was higher in Xushu 15-4 than in Xushu 15-1. Spot 2, which was newly expressed in Xushu 15-1, was identified as sporamin. Assessment of the change in protein expression levels over 8 weeks in the storage roots of the two cultivars treated at 4℃ revealed no significant difference in the expression levels in Xushu 15-1 over time. However, in Xushu 15-4, the expression level of one protein spot increased, while those of four spots decreased. Of the proteins with reduced expression levels, spots 7 and 8 were identified as actin and spots 9 and 10 were identified as fructokinase-like proteins. The present results are expected to enhance the understanding of the complex mechanism underlying the low temperature tolerance of sweet potatoes during storage and can be used to identify candidate genes for the development of new varieties of sweet potatoes with improved low temperature tolerance during cold storage in the future.
Keywords
cold storage; proteome; sweet potato; Xushu 15-1; Xushu 15-4;
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