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http://dx.doi.org/10.5333/KGFS.2005.25.4.287

Identification of Cold Stress-related Proteins in Rice Leaf Tissue  

Lee Dong-Gi (Division of Applied Life Science, Gyeongsang National University)
Lee Sang-Hoon (Division of Applied Life Science, Gyeongsang National University)
Lee Byung-Hyun (Division of Applied Life Science, Gyeongsang National University)
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.25, no.4, 2005 , pp. 287-296 More about this Journal
Abstract
To investigation protein expression pattern in rice leaves exposed to cold stress, the soluble proteins extracted from leaf tissue were fractionated with $15\%$ PEG and separated by two-dimensional polyacrylamide gel electrophoresis (2-DE). Differentially expressed proteins were identified by peptide mass fingerprinting using matrix assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS). Eight proteins up-regulated and 10 down-regulated were found in $15\%$ PEG supernatant fraction. In addition, 13 proteins up-regulated and 14 down-regulated were found in $15\%$ PEG pellet fraction. It was identified the differentially expressed proteins in $15\%$ PEG supernatant fraction as pimerase/dehydratase fructokinase, ribose-5-phosphate isomerase (Rpi), chaperonin 21 precursor, probable photosystem II oxygen-envolving complex (PS II OEC) protein 2 precursor and thioredoxin h-type (Trx-h) and those in $15\%$ PEG pellet fraction as OSINBb0059K02.15, hypothetical protein, putative mitogen-activated protein kinase kinase (MAPKK), beta 7 subunit of 205 proteasome, ribulose-1, 5-bisphosphate carboxylase/oxygenase (Rubisco) small subunit. These proteins are involved in metabolism, energy, protein synthesis, disease/defense and signal transduction-related proteins.
Keywords
Cold stress; Molecular breeding; MALDI-TOF; Proteome;
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