• Journal of Ginseng Research
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• v.32 no.1
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• pp.8-14
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• 2008

Stress activates hypothalamic-pituitary-adrenal (HPA) axis and subsequently increases the systemic levels of glucocorticoids. It also inhibits the release of gonadotropin-releasing hormone (GnRH) from hypothalamus. Korean ginseng (Panax ginseng CA Meyer) has been proven as an anti-stress agent. However, most of the anti-stress effects of ginseng on stresses such as immobilization, electronic foot shock, and cold swim, which subsequently cause oxidative damage in brain, were obtained by using ginseng extract or ginseng total saponin. Moreover, anti-stress and anti-oxidative effects of ginseng were demonstrated by determination of enzyme or hormone levels but not mRNA as well as transcriptome. Further studies on transcriptome, proteomics, and systems biology as well as signal transduction would be required to elucidate molecular action mechanisms of ginseng on stresses.

• The Transactions of the Korean Institute of Electrical Engineers B
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• v.51 no.5
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• pp.242-248
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• 2002

It is important to develop new effective technologies to increase the interruption capacity and to reduce the size of a UB(Gas Circuit Breakers). Major design parameters such as nozzle geometries and interrupting chamber dimensions affect the cooling of the arc and the breaking performance. But it is not easy to test real GCB model in practice as in theory. Therefore, a simulation tool based on a computational fluid dynamics(CFD) algorithm has been developed to facilitate an optimization of the interrupter. Special attention has been paid to the supersonic flow phenomena between contacts and the observation of hat-gas flow for estimating the breaking performance. However, there are many difficult problems in calculating the flow characteristics in a GCB such as shock wave and complex geometries, which may be either static or in relative motion. Although a number of mesh generation techniques are now available, the generation of meshes around complicated, multi-component geometries like a GCB is still a tedious and difficult task for the computational fluid dynamics. This paper presents the CFD program using CFB-CAD integration technique based on Cartesian cut-cell method, which could reduce researcher's efforts to generate the mesh and achieve the accurate representation of the geometry designed by a CAD tools.

• Journal of Embryo Transfer
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• v.20 no.1
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• pp.43-48
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• 2005

This experiment was carried out to investigate the extender, cooling rate and concentration of glycerol for freezing of boar semen. The result obtained were summarized as follows: 1. Optimal cooling rate was $0.17\~022^{\circ}C/min$ from 25 to $5^{\circ}C$ in LEY extender on the viability and normal acrosome after thawed. 2. The LEY extender was effective in protecting frozen boar semen from cold shock among the extenders(p<0.001, respectively). 3. The sperm viability and normal acrosome rates after thawing was showed greater in the 3 or $4\%$ of glycerol concentration than $2\%$ in LEY extender. 4. Viability of sperm was higher when both 15mM of fructose and 3 or $4\%$ glycerol were added to the LEY extender compared with other concentrations of fructose and glycerol were added it(p<0.001).

• Journal of Aquaculture
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• v.3 no.2
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• pp.135-144
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• 1990

Fertilized eggs of Oreochromis niloticus were subjected to cold treatments at $14^{\circ}C$ for 30, 45 and 60 minutes starting 5 minutes after insemination at $27^{\circ}C$. Ploidy levels were determined by chromosome preparations and the analysis of both cell and nuclear sizes. A temperature shock of $14^{\circ}C$ for 60 minutes yielded the best results ( $83.3\%$). Gonadal development in both sexes was severely retarded in all triploid groups at 6 months of age.

• Development and Reproduction
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• v.21 no.3
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• pp.275-286
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• 2017

The purpose of this study is to investigate the differences fatty acids and hormonal parameters in the spawning and non-spawning season between the diploid and induced triploid Far Eastern catfish, Silurus asotus. The measured triploids were produced by cold shock for 50 min at $4^{\circ}C$ in May 2014, the spawning season of diploid was in May, and the non-spawning season was designated in January. Estradiol and testosterone and gonadosomatic index of diploid were higher than those of induced triploid in spawning season (P<0.05), and those of diploid in spawning season were higher than non-spawning season. On the other hand, thyroid stimulating hormone and thyroxine of induced triploid were higher than those of diploid in spawning season (P<0.05). Erythrocyte count of diploid was higher than that of induced triploid in spawning season and non-spawning seasons. Mean corpuscular volume and mean corpuscular hemoglobin of induced triploid were higher than those of diploid in both seasons (P<0.05). Percentages of total saturated fatty acids and n-3 polyunsaturated fatty acids of induced triploid were higher than those of diploid in spawning season, but those of diploid were higher in non-spawning season (P<0.05). Percentages of total mono unsaturated fatty acids and total n-6 polyunsaturated fatty acids of diploid were higher than those of induced triploids in spawning season, while those of induced triploid in non-spawning season were higher (P<0.05). Therefore, induced triploids in the spawning season tend to concentrate on growth and lipid-synthesization, whereas, diploids concentrate on reproduction and gonadal maturation rather than on growth. In non-spawning season, growth and lipid-synthesization were not significantly different between diploid and induced triploid.

• Archives of Reconstructive Microsurgery
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• v.21 no.1
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• pp.50-55
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• 2012

Purpose: We reviewed 66 cases of the glomus tumors in the hands. The purpose of this study was to review the characteristic signs and symptoms of glomus tumors and the surgical outcomes. We made hypothesis that (1) our guideline for surgical excision are reliable and (2) our surgical technique is efficient and less harmful to the nail root. Materials and Methods: The clinical diagnosis was made with occasional radiological studies and surgical excision was performed according to our indications. Patients with electrical shock-like pain by touching (the essential symptom) and at least 2 of 4 clinical signs and symptoms (cold hypersensitivity, paroxysmal pain that radiated proximally, blue discoloration, nail deformity for dorsal tumor or a palpable nodule for pulp tumor) underwent surgical excision. Results: The duration of symptoms of the patients was about 5.3 years. No difference of prevalence was found in both hands, but the thumb and the middle finger were more affected than the other fingers. The mean follow up period was 2.4 years and none of the patients complained the same characteristic pain at the last visit. Conclusion: To the best of our knowledge, this report is the largest case series about glomus tumors in the finger tip. The clinical signs and symptoms are important to make a diagnosis of glomus tumors, and surgical excision is the treatment of choice.

• The Journal of Korean Medical History
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• v.24 no.1
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• pp.111-117
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• 2011

"Classified Assemblage of Medical Prescriptions(醫方類聚)" is a valuable material which can explain about historical records of East Asian medicine till 15th century. We can also konw what were regarded as important idea by contemporary doctors of Korean in 'Chosun' dynasty. And, Epilepsy is the one of the olddest diseases with a humankind history for a long time. All the concetps of disease including epilepsy in East Asia has been developed and specilized. So, we can find out some valuable facts from Epilepsy section in "Classified Assemblage of Medical Prescriptions". First, 'Epilepsy' was mixed with 'Mad', 'Spasm' before 7th century but after than, it had been divided into in detail. Since 14th century, we can find out that it was separated with 'Spasm' completely. In conclusion, 'Epilepsy' concept in "Classified Assemblage of Medical Prescriptions" at 15th century is similar with mordern 'Epilepsy' concept. Second, We can find out that 'Epilepsy disease' was divided into 'Five epilepsy' before 7th century, and divided into 'external cause', 'internal cause', 'external & internal cause' in 12th century. And, it also was divided into 'yin' or 'yang' epilepsy. So, 'Epilepsy' concept in " Classified Assemblage of Medical Prescriptions" at 15th century can be divided into 'yin' or 'yang' generally, and also it is similar with 'Acute and slow shock'. Especially, 'yinyang' of epilepsy is related to organs & bouls, depth & shallow, interior & exterior, not cold & heat definitely. From now on, the researches about how it had been understood in East Asia can help to find out modern significance of Korean medicine. and, it would be helpful for comprehensive study about "Classified Assemblage of Medical Prescriptions" from these researches in each section.

• Nuclear Engineering and Technology
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• v.26 no.1
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• pp.63-77
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• 1994

The objective of this work is to investigate fluid mixing phenomena related to pressurized thermal shock(PTS) in a pressurized water reactor(PWR) vessel downcomer during transient cooldown with direct vessel injection(DVI) using test models. The test model designs were based on ABB Combustion Engineering(C-E) System 80＋ reactor geometry. A cold leg small break loss-of-coolant accident(LOCA) md a main steam line teak were selected as the potential PTS events for the C-E System 80＋. This work consist of two parts. The first part provides the visualization tests of the fluid mixing between DVI fluid and existing coolant in the downcomer region, and the second part is to compare the results of thermal mixing tests with DVI in the other test model. Row visualization tests with DVI have clarified the physical interaction between DVI fluid and primary coolant during transient cooldown. A significant temperature drop was observed in the downcomer during the tests of a small break LOCA Measured transient temperature profiles agree well with the predictions by the REMIX code for a small break LOCA and with the calculations by the COMMIX-1B code for a steam line break event.

• Journal of Embryo Transfer
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• v.31 no.4
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• pp.355-365
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• 2016

The cryopreservation of sperm has become the subject of research for successful artificial insemination technologies. Antifreeze proteins (AFPs), one of the factors necessary for effective cryopreservation, are derived from certain Antarctic organisms. These proteins decrease the freezing point of water within these organisms to below the temperature of the surrounding seawater to protect the organism from cold shock. Accordingly, a recent study found that AFPs can increase the motility and viability of spermatozoa during cryopreservation. To evaluate this relationship, we performed cryopreservation of boar sperm with AFPs produced in the Arctic yeast Leucosporidium sp. AFP expression system at four concentrations (0, 0.01, 0.1, and $1{\mu}g/ml$) and evaluated motility using computer assisted sperm analysis. DNA damage to boar spermatozoa was measured by the comet assay, and sperm membrane integrity and acrosome integrity were evaluated by flow cytometry. The results showed that motility was positively affected by the addition of AFP at each concentration except $1{\mu}g/ml$ (p<0.001). Although cryopreservation with AFP decreased the viability of the boar sperm using, the tail DNA analyses showed that there was no significant difference between the control and the addition of 0.1 or $0.01{\mu}g/ml$ AFP. In addition, the percentage of live sperm with intact acrosomes showed the least significant difference between the control and $0.1{\mu}g/ml$ AFP (p<0.05), but increased with $1{\mu}g/ml$ AFP (p<0.001). Our results indicate that the addition of AFP during boar sperm cryopreservation can improve viability and acrosome integrity after thawing.

• KSBB Journal
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• v.24 no.1
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• pp.96-100
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• 2009

One of the major drawbacks associated with the high-level expression of the recombinant proteins in Escherichia coli is the formation of insoluble inclusion bodies in the cytoplasm. Production of recombinant protein at reduced temperature has proven effective in improving the solubility of a number of structurally and functionally unrelated proteins, but a major limitation of using low temperatures for recombinant protein production in E. coli is the reduced rate of synthesis of the heterologous protein caused by the significant reduction of cell growth rate. Here we investigated the effect of co-expression of CspA, a cold-shock protein known to be RNA chaperone at low temperature, on the productivity of recombinant protein at various temperatures by using green fluorescence protein (GFP) as a model recombinant protein. We could observe that the co-expression of CspA enhanced the productivity of GFP at $15^{\circ}C$ by accelerating the growth of E. coli at the temperature. On the other hand, the CspA coexpression didn't affect the cell growth rate as well as the specific GFP production rate at other tested temperatures, $20^{\circ}C$, $25^{\circ}C$, and $37^{\circ}C$.