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Studies on the Freezing of Boar Semen I. Effects of Cooling Rate and Extenders on Viability and Normal Acrosome after Frozen-Thawed of Boar Semen  

Shim K. S. (Cheonan Yonam College)
Kim K. S. (Cheonan Yonam College)
Seo K. D. (Cheonan Yonam College)
Song H. B. (Daegu University)
Publication Information
Journal of Embryo Transfer / v.20, no.1, 2005 , pp. 43-48 More about this Journal
Abstract
This experiment was carried out to investigate the extender, cooling rate and concentration of glycerol for freezing of boar semen. The result obtained were summarized as follows: 1. Optimal cooling rate was $0.17\~022^{\circ}C/min$ from 25 to $5^{\circ}C$ in LEY extender on the viability and normal acrosome after thawed. 2. The LEY extender was effective in protecting frozen boar semen from cold shock among the extenders(p<0.001, respectively). 3. The sperm viability and normal acrosome rates after thawing was showed greater in the 3 or $4\%$ of glycerol concentration than $2\%$ in LEY extender. 4. Viability of sperm was higher when both 15mM of fructose and 3 or $4\%$ glycerol were added to the LEY extender compared with other concentrations of fructose and glycerol were added it(p<0.001).
Keywords
boar; freeing; extender; glycerol; viability;
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