• 한국양식학회지
• /
• 제10권4호
• /
• pp.381-386
• /
• 1997

A series of experiments were conducted to compare the effects of various diluents in cold storage on the marbled sole, Limanda yokohamae sperm. Various diluents of glucose, L. yokohamae serum, marine fish Ringer's solution, sodium citrate and Ca2+ free artificial seawater (ASM) (tris-HCI, pH 7.4) containing 3 mM ethylene glycol-bis (2-aminoethyl ether) tetraacetic acid (EGTA) were used to store the sperm at 4℃. The storage effect was evaluated using motility index and survival rate of sperm. Glucose and sodium citrate were found to be better diluents which maintained high motility and survival rate of sperm for a storage period of 10 days. Some morphological changes of spermatozoa were observed during the cold storage with diluents. In particular, a detachment of the nuclear enveloped and of the plasma membrane from the nucleus in spermatozoa was observed. Morphological normality of the stored spermatozoa diluted with 0.3 M glucose was better than that of the stored spermatozoa undiluted or diluted with Ca2+ free ASW containing 3 mM EGTA.

• 한국수산과학회지
• /
• 제41권4호
• /
• pp.267-271
• /
• 2008

We experimentally determined the physico-chemical properties of seminal plasma as well as the sperm cryopreservation techniques of masou salmon, Oncorhynchus masou masou. Seminal plasma contained $18{\pm}1mmol/L$ potassium, $144{\pm}4mmol/L$ sodium, $116{\pm}3mmol/L$ chloride, $83.2{\pm}3.1mg/L$ calcium, $14.8{\pm}0.7mg/L$ magnesium, $45{\pm}9mg/L$ glucose, and $1.0{\pm}0.0g/L$ total protein. The osmolality and pH of seminal plasma were $287{\pm}7\;and\;7.7{\pm}0.1mmol/kg$, respectively, and the spermatocrit was $28{\pm}2$. The rate of embryonic survival at the eyed-stage and the hatching rate were highest in 10% methanol with 300 mM glucose. Compared to DMSO or glycerol, methanol served as a better cryoprotectant of masou salmon sperm.

• 한국식품과학회지
• /
• 제45권6호
• /
• pp.735-741
• /
• 2013

본 연구에 사용된 비열 플라즈마 처리는 양배추와 상추에 접종된 S. Typhimurium, 후춧가루에 존재하는 호기성 미생물, 그리고 고춧가루에 접종된 B. cereus 영양 세포를 효과적으로 저해시켰으나, 고춧가루에 존재하는 호기성균과 B. cereus 포자는 효과적으로 저해시키지 못했다. 양배추에 접종된 S. Typhimurium를 가장 많이 저해시키는 최적 조건은 746 W, 6.8분으로 예측되었고, 이 때 저해 정도는 1.9 log $CFU/cm^2$로 예측되었다. Fermi 모델과 Weibull 모델이 플라즈마 처리를 이용한 양배추에 접종된 S. Typhimurium 저해를 설명하는데 매우 적합하였다. 본 연구의 결과는 비열 플라즈마 처리를 채소류와 분말 식품의 미생물 안전성을 증진시키는 살균 처리 기술로 발전시키는데 유용할 것으로 사료된다.

• 한국식품과학회지
• /
• 제47권2호
• /
• pp.224-232
• /
• 2015

CP 처리의 CBPE 필름의 물리화학적 특성 및 생분해 특성에 대한 영향을 연구하였다. CP 처리는 CBPE 필름의 인장 강도, 신장률, 그리고 광투과도와 같은 물리적 특성에는 영향을 주지 않았으나, air-CP 처리는 CBPE 필름의 유연성, 수분 방벽 특성, 그리고 인쇄적성을 향상시켰다. Air-CP 처리는 저장 중 CBPE 필름 표면의 조도와 O와 N 원소를 포함한 작용기의 양을 증가시켰고, 인쇄적성, 열분해성, 그리고 미생물 분해성을 향상시켰다. 본 연구에서 CP 처리 후 CBPE 필름 표면에서 보여지는 노화 현상을 XPS 결과를 통해 확인할 수 있었다. 본 연구는 CP 처리가 CBPE 필름을 비롯하여 다른 생분해성 식품 포장재의 물리적 특성과 생분해성을 향상시킬 수 있는 기술로서 적용될 수 있다는 가능성을 보여주었다.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 2003년도 추계학술대회 논문집 전기물성,응용부문
• /
• pp.204-206
• /
• 2003

Electron beam generator of cold cathode type has been developed for industrial application, for example, waste water cleaning, flue gas cleaning, and pasteurization etc. The operational principle is based on the emission of secondary electrons from cold cathode when ions in the plasma hit the cathode, which are accelerated toward exit window by the gradient of an electric potential. The characteristics of secondary electron emission are studied by comparing total cathode current with ion current.

• Corrosion Science and Technology
• /
• 제6권5호
• /
• pp.251-256
• /
• 2007

Plasma nitriding is a surface treatment process which is increasingly used to improve wear, fatigue and corrosion resistance of industrial parts. Active screen plasma nitriding (ASPN) has both the advantages of the classic cold wall and the hot wall conventional dc plasma nitriding (DCPN) method and the parts to be nitrided are no longer directly exposed to the plasma. In this study, AS plasma nitriding has been used to nitride the UNS S31803 duplex stainless steel, AISI 304 and AISI 316 austenitic stainless steel, and AISI 420 martensitic stainless steel. Treated specimenswere characterized by means of microstructural analysis, microhardness measurements and electrochemical tests in NaCl aerated solutions. Hardness of the nitride cases of AISI 420 stainless steel by Knoop test can get up to 1300 HK0.1. From polarization tests, the corrosion current densities of AISI 420 and UNS S31803specimens ASPN at $420^{\circ}C$ were generally lower than those of their untreated substrates. The corrosion resistance of UNS S31803 duplex stainless steel can be enhanced by plasma nitriding at $420^{\circ}C$ Cowing to the formation of the S-phase.

• International Journal of Oral Biology
• /
• 제43권2호
• /
• pp.83-91
• /
• 2018

Nonthermal atmospheric plasma has been studied for its many biomedical effects, such as tooth bleaching, wound healing, and coagulation. In this study, the effects of dentinal tubules occlusion were investigated using fluoride-carboxymethyl cellulose (F-CMC) gel, nano-sized hydroxyapatite (n-HA), and nonthermal atmospheric plasma. Human dentin specimens were divided to 5 groups (group C, HA, HAF, HAP, and HAFP). Group HA was treated with n-HA, group HAF was treated with n-HA after a F-CMC gel application, group HAP was treated with n-HA after a plasma treatment and group HAFP was treated with n-HA after a plasma and F-CMC gel treatment. The occlusion of dentinal tubules was investigated using scanning electron microscopy (SEM) and energy dispersive x-ray spectroscopy (EDS), which shows Ca/P ratio. In the EDS results, a higher Ca/P ratio was shown in the groups including n-HA than in the control group. The specimens of group HAP and HAFP had a higher Ca/P ratio in retentivity. In the SEM results, there was not a significant difference in the amount of times applied. Therefore, this study suggests F-CMC gel and n-HA treatment using nonthermal atmospheric plasma will be a new treatment method for decreasing hypersensitivity.

• 대한전자공학회논문지
• /
• 제5권3호
• /
• pp.31-39
• /
• 1968

외부자계가 가해진 냉프라즈마에서의 마이크로파 전파 특성을 측정하였다. 직류방전프라즈마는 두 전극판을 구형도파관에 삽입한것과, 또한 유리시설관을 구형도파관에 삽입한 것에서 이루어졌다. 마이크로파 전파방향, 방전관축, 외부자계는 각각 수직이고, 자속밀도, 방전유기, 기체압등이 증가할때 프라즈마를 전파하는 마이크로파의 감쇠 및 흡수는 증가함을 보았다.

• Asian-Australasian Journal of Animal Sciences
• /
• 제33권9호
• /
• pp.1444-1454
• /
• 2020

Objective: Cold stress induces oxidative damage and impairs energy status of broilers. N-acetylcysteine (NAC) exhibits antioxidant properties and modulates energy metabolism of animals. This study was conducted to investigate the effects of NAC on energy status and antioxidant capacity of heart and liver in the cold-stressed broilers. Methods: The experiment consisted of 4 treatments in a 2×2 factorial arrangement with two diets (basal diet or plus 0.1% NAC) and two ambient temperatures (thermoneutral [conventional ambient temperature] or cold stress [10℃±1℃ during days 15 to 42]). Results: No ascites were seen in cold-stressed broilers. NAC did not attenuate the impaired growth performance of stressed birds. However, NAC decreased plasma asparagine but increased aspartate levels in cold-stressed birds (p<0.05). NAC reduced hepatic adenosine triphosphate (ATP) but elevated adenosine diphosphate contents in unstressed birds (p<0.05). The hepatic ratio of adenosine monophosphate (AMP) to ATP was increased in birds fed NAC (p<0.05). NAC decreased plasma malondialdehyde (MDA) level and cardiac total superoxide dismutase (T-SOD) activity in unstressed birds, but increased hepatic activities of T-SOD, catalase and glutathione peroxidase in stressed birds (p<0.05). NAC down-regulated hepatic AMP-activated protein kinase but up-regulated cardiac heme-oxigenase mRNA expression in stressed birds, and decreased expression of hepatic peroxisome proliferator-activated receptor coactivator-1α as well as hypoxia-inducible factor-1α in liver and heart of birds. Conclusion: Dietary NAC did not affect energy status but enhanced the hepatic antioxidant capacity by increasing the activities of antioxidant enzymes in cold-stressed broilers.

• 천문학회보
• /
• 제37권1호
• /
• pp.97-97
• /
• 2012

Hinode/XRT has observed coronal mass ejections (CMEs) since it launched on Sep. 2006. Observing programs of Hinode/XRT, called 'CME watch', perform several binned observations to obtain large FOV observations with long exposure time that allows the detection of faint CME plasmas in high temperatures. Using those observations, we determine the upper limit to the mass of hot CME plasma using emission measure by assuming the observed plasma structure. In some events, an associated prominence eruption and CME plasma were observed in EUV observations as absorption or emission features. The absorption feature provides the lower limit to the cold mass while the emission feature provides the upper limit to the mass of observed CME plasma in X-ray and EUV passbands. In addition, some events were observed by coronagraph observations (SOHO/LASCO, STEREO/COR1) that allow the determination of total CME mass. However, some events were not observed by the coronagraphs possibly because of low density of the CME plasma. We present the mass constraints of CME plasma and associated prominence as determined by emission and absorption in EUV and X-ray passbands, then compare this mass to the total CME mass as derived from coronagraphs.