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http://dx.doi.org/10.5657/kfas.2008.41.4.267

Effects of Diluents and Cryoprotectants on Sperm Cryopreservation of Mason Salmon, Oncorhynchus masou masou  

Lim, Han-Kyu (Aquaculture Management Division, National Fisheries Research and Development Institute)
Lee, Cheul-Ho (Cold-Water Inland Fisheries Research Institute)
Min, Byung-Hwa (Aquaculture Management Division, National Fisheries Research and Development Institute)
Lee, Jung-Uie (Aquaculture Management Division, National Fisheries Research and Development Institute)
Lee, Chae-Sung (Cold-Water Inland Fisheries Research Institute)
Seong, Ki-Baik (Cold-Water Inland Fisheries Research Institute)
Lee, Sang-Mok (Aquaculture Management Division, National Fisheries Research and Development Institute)
Publication Information
Korean Journal of Fisheries and Aquatic Sciences / v.41, no.4, 2008 , pp. 267-271 More about this Journal
Abstract
We experimentally determined the physico-chemical properties of seminal plasma as well as the sperm cryopreservation techniques of masou salmon, Oncorhynchus masou masou. Seminal plasma contained $18{\pm}1mmol/L$ potassium, $144{\pm}4mmol/L$ sodium, $116{\pm}3mmol/L$ chloride, $83.2{\pm}3.1mg/L$ calcium, $14.8{\pm}0.7mg/L$ magnesium, $45{\pm}9mg/L$ glucose, and $1.0{\pm}0.0g/L$ total protein. The osmolality and pH of seminal plasma were $287{\pm}7\;and\;7.7{\pm}0.1mmol/kg$, respectively, and the spermatocrit was $28{\pm}2$. The rate of embryonic survival at the eyed-stage and the hatching rate were highest in 10% methanol with 300 mM glucose. Compared to DMSO or glycerol, methanol served as a better cryoprotectant of masou salmon sperm.
Keywords
Cryoprotectant; Diluent; Oncorhynchus masou masou; Sperm cryopreservation;
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Times Cited By KSCI : 2  (Citation Analysis)
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