• Proceedings of the Microbiological Society of Korea Conference
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• 2001.11a
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• pp.97-101
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• 2001

The fusion between viral envelope and target cell membrane is a central step of viral infection, and the fusion proteins located at viral envelope mediate such process. Gp41 of HIV is one of the fusion proteins whose structure and mechanism of membrane fusion had been extensively studied. Functionally important motives of gp41 are the N-terminus fusion peptide, the coiled-coil and the membrane proximal C-peptide regions. The role of these regions during the fusion process had been thoroughly examined. Specially, insertion of the fusion peptide into membrane and conformational change of the coiled-coil and C-peptide regions are assumed to be critical for the fusion mechanism. In addition, the coiled-coil region has been shown to interact with membrane, and the C-peptide region regulates the interaction in a dose dependent manner. Furthermore, fusion defective mutations of the coiled-coil region dramatically changed its binding affinity to membrane. These results suggested that the membrane binding property of the coiled-coil region is important for the fusion activity of gp41, and such property could be modulated by the interaction with the C-peptide region.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.10 no.3
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• pp.282-291
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• 1998

Heat transfer performance are studied for the turbulent flow of water in 3 smooth tube coils having ratios of coil to tube diameter of 16, 21 and 27, and a corrugated-coiled tube having a ratio of coil to tube diameter of 29, for Reynolds numbers from 8000 to 60000 and is also compared with the limited results available to data. The experiments are carried out for the fully developed turbulent flow of water in tube coils under the condition of uniform heat flux. This work is limited 0 tube coils of R/a between 10 and 30. The tube having a ratio of coil to tube diameter of 27 among the 3 smooth tube coils shows the best heat transfer performance. The performance of coiled tube best transfer performance. The performance of coiled tube with a similar curvature ratio is better for a corrugated-coiled tube(R/a=17) than for a smooth coiled tube(R/a=16). An empirical relation which correlates most of the data within $\pm$25% was also developed. Test result shows that the Nusselt number is found to be affected by a secondary flow due to curvature.

• Journal of Mechanical Science and Technology
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• v.20 no.2
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• pp.212-226
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• 2006

This paper is a continuation of the authors' previous work on spiral coil heat exchangers. In the present study, the heat transfer characteristics and the performance of a spirally coiled finned tube heat exchanger under wet-surface conditions are theoretically and experimentally investigated. The test section is a spiral-coil heat exchanger which consists of a steel shell and a spirally coiled tube unit. The spiral-coil unit consists of six layers of concentric spirally coiled finned tubes. Each tube is fabricated by bending a 9.6 mm diameter straight copper tube into a spiral-coil of four turns. The innermost and outermost diameters of each spiral-coil are 145.0 and 350.4 mm, respectively. Aluminium crimped spiral fins with thickness of 0.6 mm and outer diameter of 28.4 mm are placed around the tube. The edge of fin at the inner diameter is corrugated. Air and water are used as working fluids in shell side and tube side, respectively. The experiments are done under dehumidifying conditions. A mathematical model based on the conservation of mass and energy is developed to simulate the flow and heat transfer characteristics of working fluids flowing through the heat exchanger. The results obtained from the present model show reasonable agreement with the experimental data.

• Transactions of the Korean Society of Automotive Engineers
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• v.6 no.6
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• pp.155-165
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• 1998

The resistance coefficient and heat transfer performance are studied for the turbulent water flow in a smooth coiled tube having variable curvature ratios and a corrugated-coiled tube having a ratio of coil to tube diameter of 22. Experiments are carried out for the fully developed turbulent flow of water in tube coils on the uniform wall temperature condition. This work is limited to tube coils of R/a between 22 and 60 and Reynolds numbers from 13000 to 53000. The tube having a ratio of coil to tube diameter of 27 among the 3 smooth tube coils shows the best heat transfer performance. A corrugated-coiled tube(R/a=60) shows more excellent performance than a smooth coiled tub (R/a=60) at a similar curvature ratio. The friction factor f is sensitive to changes in the velocity profile caused by a temperature gradient. Allowance was made for the pressure loss in the short inlet and outlet lengths and due to the presence of the thermocouple inlet and outlet as a result of separate experimental on a straight tube. It is to be expected that the allowance at the exit will be somewhat too low because of secondary flow effects carried over from the coil.

• Journal of Mechanical Science and Technology
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• v.16 no.11
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• pp.1540-1549
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• 2002

Experimental results are presented for the effects of coil diameter, system pressure and mass flux on dryout pattern of two-phase flow in helically coiled tubes. Two tubes with coil diameters of 215 and 485 mm are used in the present study, Inlet system pressures range from 0.3 to 0.7 MPa, mass flux from 300 to 500 kg/㎡s, and heat flux from 36 to 80 kw/㎡. A partial dryout region exists because of the geometrical characteristics of the helically coiled tube. The length of the partial dryout region increases with coil diameter and system pressure. On the other hand, it decreases with increasing mass flux. The critical quality at the tube top side increases with mass flux, but decreases with increasing system pressure. This tendency is more notable when the coil diameter is larger. When the centrifugal force effect becomes stronger, dryout starts at the top and bottom sides of the tube. However, when the gravity effect becomes stronger, dryout is delayed at the tube bottom side. In some cases when the mass flux is low, dryout occurs earlier at the outer side than at the inner side of the tube because of film inversion.

• Journal of Electrical Engineering and Technology
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• v.9 no.1
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• pp.162-169
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• 2014

The analysis and comparation of the half-coiled short-pitch windings with different phase belt are presented in the paper. The half-coiled short-pitch windings can supply the odd and even harmonics simultaneously, which can be applied in multiphase bearingless motor (MBLM). The space harmonic distribution of the half-coiled short-pitch windings with two kinds of phase belt is studied wi th respect to different coil pitch, and the suitable coil pitch can be selected from the analysis results to reduce the additional radial force and torque pulse. The two kinds of half-coiled short-pitch windings are applied to the five- and six-phase bearingless motor, and the comparation from the Finite Element Method (FEM) results shows that the winding with $2{\pi}/m$ phase belt is fit for the five phase bearingless motor and the winding with ${\pi}/m$ phase belt is suitable for the six phase bearingless motor. Finally, a five phase surface-mounted permanent magnet (PM) bearingless motor is built and the experimental results are presented to verify the validity and feasibility of the analysis. The results presented in this paper will give useful guidelines for design optimization of the MBLM.

• Proceedings of the Korean Society for Technology of Plasticity Conference
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• 2002.05a
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• pp.39-43
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• 2002

A new model for heat transfer and thermal deformation analysis according to strip mm in coiling process has been proposed. Finite difference analyses for heat transfer of cold rolled coil have been carried out under various coiling tensions and strip crown using the equivalent thermal conductivity for the radial direction of cold rolled coil which is a function of strip thickness, surface characteristics and compressive pressure. The compressive pressure is calculated from a equation expressed as a function of hoop stress and coil tension considering strip mm obtained by experiment. Finite element method for thermal deformation of cold rolled coil has been performed to investigate the effects of the strip crown, the coil tension and temperature. From these analyses, it is found that the axial inhomogeneity of thermal deformation is increased as the strip crown, compressive pressure, and temperature drop in cold coiled strip increase.

• Korean Journal of Veterinary Research
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• v.55 no.3
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• pp.181-184
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• 2015

Ciliary rootlet coiled coil protein (CROCC), the structural component that originates from the basal body at the proximal end of the ciliary rootlet, plays a crucial role in maintaining the cellular integrity of ciliated cells. In the current study, we cloned Xenopus CROCC and performed the expression analysis. The amino acid sequence of Xenopus laevis was related to those of Drosophila, cow, goat, horse, chicken, mouse and human. Reverse transcription polymerase chain reaction analysis revealed that CROCC mRNA encoding a coiled coil protein was present maternally, as well as throughout early development. In situ hybridization indicated that CROCC mRNA occurred in the animal pole of embryo during gastrulation and subsequently in the presumptive neuroectoderm at the end of gastrulation. At tailbud stages, CROCC mRNA expression was localized in the anterior roof plate of the developing brain, pharyngeal epithelium connected to gills, esophagus, olfactory placode, intestine and nephrostomes of the pronephric kidney. Our study suggests that CROCC may be responsible for control of the development of various ciliated organs.

• Journal of Microbiology and Biotechnology
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• v.17 no.10
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• pp.1607-1615
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• 2007

We investigated the applicability of the TEM-l ${\beta}$-lactamase fragment complementation (BFC) system to develop a strategy for the screening of protein-protein interactions in bacteria. A BFC system containing a human Fas-associated death domain (hFADD) and human Fas death domain (hFasDD) was generated. The hFADD-hFasDD interaction was verified by cell survivability in ampicillin-containing medium and the colorimetric change of nitrocefin. It was also confirmed by His pull-down assay using cell lysates obtained in selection steps. A coiled-coil helix coiled-coil domain-containing protein 5 (CHCH5) was identified as an interacting protein of human uracil DNA glycosylase (hUNG) from the bacterial BFC cDNA library strategy. The interaction between hUNG and CHCH5 was further confirmed with immunoprecipitation using a mammalian expression system. CHCH5 enhanced the DNA glycosylase activity of hUNG to remove uracil from DNA duplexes containing a U/G mismatch pair. These results suggest that the bacterial BFC cDNA library strategy can be effectively used to identify interacting protein pairs.

• Journal of Life Science
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• v.19 no.7
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• pp.859-865
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• 2009

Microtubules, a major cytoskeleton, form parallel arrays in the axon and are oriented with their plus ends toward the cell periphery. Kinesin superfamily proteins (KIFs) are the molecular motors acting in the microtubule-based motilities of organelles in cells. Here, we used the yeast two-hybrid system to identify the protein that interacts with the coiled-coil domain of KIF1A and found a specific interaction with microtubule-destabilizing factor SCG10. SCG10 bound to the amino acid residues between 400 and 820 of KIF1A, but not to other KIFs in the yeast two-hybrid assay. The coiled-coil domain of SCG10 is essential for interaction with KIF1A. In addition, this specific interaction was also observed in the Glutathione S-transferase pull-down assay. An antibody to SCG10 specifically co-immunoprecipitated KIF1A associated with SCG10 from mouse brain extracts. These results suggest that KIF1A motor protein transports SCG10-containing vesicles along microtubules in neurons.