• Molecules and Cells
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• 제26권6호
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• pp.530-535
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• 2008

Optical signals based on Raman scattering, coherent anti-Stokes Raman scattering (CARS), and harmonic generation can be used to image biological molecules in living cells without labeling. Both Raman scattering and CARS signals can be used to detect frequencies of molecular vibrations and to obtain the molecular distributions in samples. Second-harmonic optical signals can also be generated in structured arrays of noncentrosymmetric molecules and can be used to detect structured aggregates of proteins, such as, collagen, myosin and tubulin. Since labeling techniques using chemical and biological reactions may cause undesirable changes in the sample, label-free molecular imaging techniques are essential for observation of living samples.

• Journal of the Optical Society of Korea
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• 제14권4호
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• pp.424-430
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• 2010

In coherent anti-Stokes Raman scattering (CARS) microscopy reported until now, conventional microscope objectives are used, so that they are limited for introduction into a living body. Gradient-index (GRIN) rod lenses might be a solution for miniaturized microscope objectives for in-vivo CARS microscopy. However, due to the inherent large amount of chromatic aberration, GRIN rod lenses cannot be utilized for this purpose. CARS imaging catheter, composed of miniaturized microscope objective and fiber bundle, can be introduced into a living body for minimally invasive diagnosis. In order to design the catheter, we have to first investigate design requirements. And then, the optical design is processed with design strategies and intensive computing power to achieve the design requirements. We report the miniaturized objective lens system with diffraction-limited performance and completely corrected chromatic aberrations for an in-vivo CARS imaging catheter.

• 한국광학회:학술대회논문집
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• 한국광학회 2007년도 하계학술발표회 논문집
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• pp.31-32
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• 2007

• Journal of the Optical Society of Korea
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• 제14권4호
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• pp.431-437
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• 2010

A stack of gradient-index (GRIN) rod lenses cannot be used for coherent anti-Stokes Raman scattering (CARS) microendoscopy for insertion to internal organs through a surgical keyhole with minimal invasiveness. That's because GRIN lens has large amount of inherent chromatic aberrations in spite of absolutely requiring a common focus for pump and Stokes beam with each frequency of ${\omega}_p$ and ${\omega}_S$. For this endoscopic purpose, we need to develop a long slender probe-type objective, namely probe-type microscope objective (PMO). In this paper, we introduce the structure, the working principle, and the design techniques of PMO which is composed of a probe-type lens module (PLM) and an adaptor lens module (ALM). PLM is first designed for a long slender type and ALM is successively designed by using several design parameters from PLM for eliminating optical discords between scanning unit and PLM. A combined module is optimized again to eliminate some coupling disparities between PLM and ALM for the best PMO. As a result, we can obtain a long slender PMO with perfectly diffraction-limited performance for pump beam of 817 nm and Stokes beam of 1064 nm.

• 한국광학회지
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• 제18권4호
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• pp.286-291
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• 2007

높은 수치구경의 대물렌즈를 사용하는 간섭성 반스톡스 라만 산란 현미경(coherent anti-Stokes Raman scattering microscopy)에서 폴리스틸렌구에서 발생한 신호의 먼거리장 방사패턴에 대한 이론적 계산 연구를 수행하였다. 극초점 조건에서 입사 레이저 광의 전기장 분포를 계산하였고, CARS 신호 생성원인인 비선형 분극(헤르치안 쌍극자) 방사의 간섭성 합을 통하여 먼거리장 방사 패턴을 계산하였다. 폴리스틸렌구의 크기에 따른 후방 방사패턴을 계산하였고, 1100 nm 직경을 가진 폴리스틸렌구와 폴리스틸렌 구껍질의 방사패턴을 비교하였다. 또한, 극초점으로부터 폴리스틸렌구의 중심이 이동함에 따른 방사패턴의 변화를 보였다.

• 한국광학회:학술대회논문집
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• 한국광학회 2002년도 하계학술발표회
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• pp.222-223
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• 2002

3차 비선형 광학현상을 이용한 레이저 분광학은 코헤런트 반스톡스 라만산란(Coherent Anti-Stokes Raman Scattering, CARS)이나 축퇴 사광자혼합(Degenerate Four-Wave Mixing, DFWM)이 기계공학의 연소진단이나 화학분야에 응용된 이래 활발히 연구되어져왔다.[1] 비선형 광학현상의 특성상, 발생한 신호는 입사 레이저광들과의 위상정합조건이 만족되는 특정한 방향으로만 진행하고 레이저광처럼 가간섭성을 갖는다. (중략)

• 대한기계학회논문집
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• 제19권5호
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• pp.1333-1339
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• 1995

Recently developed technique of measuring minor species concentration by using the modulation dip in broadband CARS has been applied to the flame structure study of methane/air premixed flames in a counterflow. This method used the modulation dip from the cold band CO Q-branch resonant signal superimposed on the nonresonant background. The measured CO concentration profile in a symmetric and unsymmetric methane/air premixed flames together with the velocity and temperature by using LDV and CARS have been compared with the numerical results adopting detailed chemistry modeling. The results show that there is a satisfactory agreement between the experimental data and numerical results for velocities, temperatures and CO concentrations. And the modulation dip technique of measuring minor species, such as CO is a viable tool for a quantitative measurement in a flame.

• Applied Science and Convergence Technology
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• 제24권6호
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• pp.203-214
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• 2015

We have been developing a new label-free nanobio imaging platform using non-linear optics such as Coherent Anti-Stokes Raman Spectroscopy (CARS) and ion beam techniques based on sputtering and scattering such as Secondary Ion Mass Spectrometry (SIMS) and Medium Energy Ion Scattering Spectroscopy (MEIS), which have been widely used for atomic and molecular level analysis of semiconductors and nanomaterials. To apply techniques developed for semiconductors and nanomaterials for biomedical applications, the convergence of nano-analysis and biology were tried. Our activities on label-free nanobio imaging during the last decade are summarized in this review about non-linear optical 3D imaging, ellipsometric interface imaging, SIMS imaging, and TOF-MEIS nano analysis for cardiovascular tissues, collagen thin films, peptides on microarray, nanoparticles, and cell adhesion studies and finally the present snapshot of nanobio imaging and the future prospect are described.

• Bulletin of the Korean Chemical Society
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• 제16권9호
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• pp.810-813
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• 1995

Coherent anti-Stokes Raman scattering (CARS) spectra of HCl, N2, and the ν1 fundamental of C2H2 have been measured in the gas phase. The measured spectra show rotational structures which originate from the Q-branch transitions. The spectra have successfully been simulated with proper selection rules, line positions, and relative intensities from room temperature Boltzmann population distributions. The vibration-rotation interaction constant α of HCl in the ground electronic state has been measured from the rotationally resolved CARS spectra which is α=0.3076 cm-1. Possibilities of optical pumping and of measuring state specific energy distributions of molecules are discussed.

• 한국광학회지
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• 제7권2호
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• pp.142-149
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• 1996

대향류버너의 화염에서 연소반응의 중간단계 생성물로 존재하는 OH라디칼의 축퇴 4광자 혼합스팩트럼을 비포화영역에서 Forward Box 형태의 위상정합조건으로 측정하였다. X$^{2}$.PI..rarw.A$^{2}$.SIGMA.$^{+}$ 천이선들 중 R$_{1}$-가지 분광선들의 세기로부터 회전에너지 준위의 밀도분포를 구하고 볼쯔만분포와 비교하여 온도를 구하였다. 흡수분광스펙트럼을 측정하여 화염내에서 레이저의 흡수효과를 보정하여 주었다. 측정된 온도의 오차는 2000K 근방에서 .+-.60K로서 CARS로 얻은 온도와 오차범위내에서 일치하였다. 화염의 여러부분에서 스펙트럼을 측정하여 온도분포와 OH농도분포를 얻었다.