• Title/Summary/Keyword: Coding gain

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Construction of fat1 Gene Expression Vector and Its Catalysis Efficiency in Bovine Fetal Fibroblast Cells

  • Liu, Boyang;Yang, Runjun;Li, Junya;Zhang, Lupei;Liu, Jing;Lu, Chunyan;Lian, Chuanjiang;Li, Zezhong;Zhang, Yong-Hong;Zhang, Liying;Zhao, Zhihui
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.5
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    • pp.621-628
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    • 2012
  • The FAT-1 protein is an n-3 fatty acid desaturase, which can recognize a range of 18- and 20-carbon n-6 substrates and transform n-6 polyunsaturated fatty acids (PUFAs) into n-3 PUFAs while n-3 PUFAs have beneficial effect on human health. Fat1 gene is the coding sequence from Caenorhabditis elegans which might play an important role on lipometabolism. To reveal the function of fat1 gene in bovine fetal fibroblast cells and gain the best cell nuclear donor for transgenic bovines, the codon of fat1 sequence was optimized based on the codon usage frequency preference of bovine muscle protein, and directionally cloned into the eukaryotic expression vector pEF-GFP. After identifying by restrictive enzyme digests with AatII/XbaI and sequencing, the fusion plasmid pEF-GFP-fat1 was identified successfully. The pEF-GFP-fat1 vector was transfected into bovine fetal fibroblast cells mediated by Lipofectamine2000$^{TM}$. The positive bovine fetal fibroblast cells were selected by G418 and detected by RT-PCR. The results showed that a 1,234 bp transcription was amplified by reverse transcription PCR and the positive transgenic fat1 cell line was successfully established. Then the expression level of fat1 gene in positive cells was detected using quantitative PCR, and the catalysis efficiency was detected by gas chromatography. The results demonstrated that the catalysis efficiency of fat1 was significantly high, which can improve the total PUFAs rich in EPA, DHA and DPA. Construction and expression of pEF-GFP-fat1 vector should be helpful for further understanding the mechanism of regulation of fat1 in vitro. It could also be the first step in the production of fat1 transgenic cattle.

A Grounded Theory Approach on Peoples' Adaptation Experience with Fibromyalgia Syndrome (섬유근통증후군 환자의 질병 적응경험에 관한 근거이론 연구)

  • Jeong, Chu-Yeong;Kim, Myung-Hee
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.17 no.12
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    • pp.381-393
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    • 2016
  • This was a qualitative study to explore and better understand the adaptation experience and processes of peoples with fibromyalgia syndrome (FMS), as well as to develop a substantive theory using the grounded theory method. There were 13 patients (12 females and 1 male) who received FMS treatment from Rheumatic Medicine outpatient department of one general hospital. The data were collected through an in-depth interview between January and May of 2014. Transcribed interview contents were analyzed by the grounded theory method of Corbin and Strauss (2008). As a result, a total of 98 concepts, 26 sub-categories, and 10 categories were identified through the open coding process. The process of adaptation experience showed 4 steps: perception of uncertainty and limited condition, evaluation of self-control possibility and determinations of expectations of life, searching and trying of strategies, as well as self-regulation. The 4 types of adaptation experience were expansionary, complacently, effusively and withering. The 'protective self-regulation' theory was derived from the core category of 'learning to self-regulation method'. Patients with FMS has repeatedly attempted these strategies of protective self-regulation in order to gain stability from uncertainty and limited condition of the disease. Based on these results, it is necessary to develop an educational program for patients and families which has appropriate nursing intervention strategies in accordance with the types of adaptation.

The Effect of Interleukin $1-{\beta}$, Platelet Derived Growth Factor-BB and Transforming Growth $Factor-{\beta}$ on the expression of PDLs17 mRNA in the Cultured Human Periodontal Ligament Fibroblasts (($IL-1{\beta}$), PDGF-BB 그리고 $TGF-{\beta}$가 사람 배양 치주인대 섬유모세포의 PDLs17 mRNA의 발현에 미치는 영향)

  • Lirn, Ki-Jung;Han, Kyung-Yoon;Kirn, Byung-Ock;Yeorn, Chang-Yeob;Park, Joo-Cheol
    • Journal of Periodontal and Implant Science
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    • v.31 no.4
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    • pp.787-801
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    • 2001
  • The molecular mechanisms control the function of PDL(periodonta1 ligament) cells and/or fibroblasts remain unclear. PDLsl7, PDL-specific gene, had previousely identified the cDNA for a novel protein from cultured PDL fibroblasts using subtraction hybridization between gingival fibroblasts and PDL fibroblasts. The purpose of this study was to determine the regulation by growth factors and cytokines on PDLsl7 gene expression in cultured human periodontal ligament cells and observe the immunohistochemical localization of PDLsl7 protein in various tissues of mouse. Primary PDL fibroblasts isolated by scraping the root of the extracted human mandibular third molars. The cells were incubated with various concentration of human recombinant $IL-1{\beta}$, PDGF-BB and TGF\;${\beta}$ for 48h nd 2 weeks. At each time point total RNA was extracted and the levels of transcription ere assessed by reverse transcription-polymerase chain reaction (RT-PCR assay). polyclonal antiserum raised against PDLsl7 peptides, CLSVSYNRSYQINE and SEAVHETDLHDGC, were made, and stained the tooth, periodontium, developing bone, bone marrow and mid-palatal suture of the mouse. The results were as follows. 1. PDLsl7 mRNA levels were increased in response to PDGF (10ng/ml) and $TGF\;{\beta}$(20ng/ml) after treatment of the $IL-1{\beta}$, PDGF-BB and $TGF{\beta}$for 48 h. 2. PDLsl7 was up-regulated only by $TGF{\beta}$(20 ng/ml) after treatment of the $IL-1{\beta}$, PDGF-BB and $TGF\;{\beta}$ for 2 weeks and unchanged by the other stimulants. 3. PDLsl7 was a novel protein coding the 142 amino acid peptides in the ORF and the nucleotide sequences of the obtained cDNA from RT-PCR was exactly same as the nucleotides of the database. 4. Immunohistochemical analysis showed that PDLsl7 is preferentially expressed in the PDL, differentiating osteoblast-like cells and stromal cells of the bone marrow in the adult mouse. 5. The expression of PDLsl7 protein was barely detectable in gingival fibroblasts, hematopoetic cells of the bone marrow and mature osteocytes of the alveolar bone. These results suggest that PDLsl7 might upregulated by PDGF-BB or $TGF{\beta}$ and acts at the initial stage of differentiation when the undifferentiated mesenchymal cells in the bone marrow and PDL differentiate into multiple cell types. However, more research needs to be performed to gain a better understanding of the exact function of PDLsl7 during the differentiation of bone marrow mesenchymal and PDL cells.

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Error Performance Analysis of DS/CDMA-Trellis Coded QPSK Signal with MRC Diversity Reception in Wireless Data Communication (무선 데이터 통신에서 MRC 다이버시티 수신시의 DS/CDMA-Trellis Coded QPSK 신호의 오율 해석)

  • 노재성;김영철;박기식;강희조;조성준
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.9 no.3
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    • pp.317-329
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    • 1998
  • In this paper, we have analyzed the packet error probability of DS/CDMA-Trellis Coded QPSK modulation signal with MRC(Maximum Ratio Combining) diversity reception in Rician fading, multi-user interferences and multipath channel. And then we have evaluated the performance and capacity of DS/CDMA-Trellis Coded QPSK system using the MRC diversity reception as a function of direct power to indirect power ratio ($K_R$), the number of diversity branch(M), the number of multi-user(U), PN chip rate(PN), the number of multipath channel($L_P$), and packet length(PL). From the results, we know that the coding gain of DS/CDMA-Trellis Coded QPSK system with 2 branch MRC diversity is about 6 dB against uncoded DS/CDMA BPSK system with 2 branch MRC diversity in Rician fading ($K_R=6dB), 5 multi-user interferences, and 3 multipath channel. And, we know that coded QPSK signal designed for the AWGN channel also perform well on a Rician fading channel with MRC diversity reception. Consequently, we expected that proposed system structure is reliable to the wireless data communication system in Rician fading, multi-user interferences, and multipath channel.

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The Impact of Mergers on the Experiences of Students (대학 통폐합에 대한 학생들의 적응과정 연구)

  • Chae, Jae-Eun
    • Korean Journal of Comparative Education
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    • v.23 no.5
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    • pp.53-75
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    • 2013
  • This article explores the impact of mergers on the experiences of students. A case design is used to gain an in-depth understanding of the students in a Korean university that was combined with a two-year college in 2007. Semi-structured interviews of 11 direct entrants, 5 transfer students, and 7 professors were conducted in 2010 for the data collection. The results suggest that mergers do not necessarily ensure the integration of the students involved. Since mergers pose enormous challenges to both transfer students and direct entrants, university leaders need to give careful attention to students' persistence and integration as the campus changes. The results also reveal that students' experience of mergers may be affected by cultural backgrounds as well as the hierarchical structure of higher education. In Korean society, where credentialism prevails, mergers can have different impacts on students who come from initial institutions of differing perceived quality, and thus mergers may incite tension among the students. In light of these concerns, this study's findings highlight the importance of student integration as a key element in mergers.

Analysis of Feature Map Compression Efficiency and Machine Task Performance According to Feature Frame Configuration Method (피처 프레임 구성 방안에 따른 피처 맵 압축 효율 및 머신 태스크 성능 분석)

  • Rhee, Seongbae;Lee, Minseok;Kim, Kyuheon
    • Journal of Broadcast Engineering
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    • v.27 no.3
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    • pp.318-331
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    • 2022
  • With the recent development of hardware computing devices and software based frameworks, machine tasks using deep learning networks are expected to be utilized in various industrial fields and personal IoT devices. However, in order to overcome the limitations of high cost device for utilizing the deep learning network and that the user may not receive the results requested when only the machine task results are transmitted from the server, Collaborative Intelligence (CI) proposed the transmission of feature maps as a solution. In this paper, an efficient compression method for feature maps with vast data sizes to support the CI paradigm was analyzed and presented through experiments. This method increases redundancy by applying feature map reordering to improve compression efficiency in traditional video codecs, and proposes a feature map method that improves compression efficiency and maintains the performance of machine tasks by simultaneously utilizing image compression format and video compression format. As a result of the experiment, the proposed method shows 14.29% gain in BD-rate of BPP and mAP compared to the feature compression anchor of MPEG-VCM.

A Study on the Scheme of Implementing Meta-data Based Applications for Enterprises (메타 데이터 기반의 기업용 애플리케이션 구축 방안에 관한 연구)

  • Jang, Gil-Sang
    • Journal of the Korea Society of Computer and Information
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    • v.14 no.9
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    • pp.135-145
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    • 2009
  • Generally, the phases of constructing information systems are consisted of systems planning and selection, system analysis, system design, and system implementation and operation. These systems require many efforts and costs for additional development of modification requirements due to a frequent changes of business environments and business processes. Especially, inconsistencies between system design and system implementation usually happen during development steps because of the difficulties of program developments due to difficulties of capturing exact user requirements and frequent changes of user requirements. This paper proposes a scheme of implementing meta-data based applications for enterprises in order to reduce inconsistencies between system design and system implementation and to overcome limits of the existing coding-based development methods of applications which must use until disuse if they are developed once. Also, this paper presents a framework of repository system to systematically manage and utilize meta-data. The core concept of the proposed scheme makes outputs generated in the phases of system analysis and design into meta-data and is to easily develop and customize application programs using meta-data repository. Also, to show the applicability of the proposed scheme, it is applied to implement ERP system of 'H' automotive part manufacturer. As a result, the proposed scheme can gain improvements such as easiness and productivity of program development, easiness of maintenance, reusability of program components, etc.

A Review of the Neurocognitive Mechanisms for Mathematical Thinking Ability (수학적 사고력에 관한 인지신경학적 연구 개관)

  • Kim, Yon Mi
    • Korean Journal of Cognitive Science
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    • v.27 no.2
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    • pp.159-219
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    • 2016
  • Mathematical ability is important for academic achievement and technological renovations in the STEM disciplines. This study concentrated on the relationship between neural basis of mathematical cognition and its mechanisms. These cognitive functions include domain specific abilities such as numerical skills and visuospatial abilities, as well as domain general abilities which include language, long term memory, and working memory capacity. Individuals can perform higher cognitive functions such as abstract thinking and reasoning based on these basic cognitive functions. The next topic covered in this study is about individual differences in mathematical abilities. Neural efficiency theory was incorporated in this study to view mathematical talent. According to the theory, a person with mathematical talent uses his or her brain more efficiently than the effortful endeavour of the average human being. Mathematically gifted students show different brain activities when compared to average students. Interhemispheric and intrahemispheric connectivities are enhanced in those students, particularly in the right brain along fronto-parietal longitudinal fasciculus. The third topic deals with growth and development in mathematical capacity. As individuals mature, practice mathematical skills, and gain knowledge, such changes are reflected in cortical activation, which include changes in the activation level, redistribution, and reorganization in the supporting cortex. Among these, reorganization can be related to neural plasticity. Neural plasticity was observed in professional mathematicians and children with mathematical learning disabilities. Last topic is about mathematical creativity viewed from Neural Darwinism. When the brain is faced with a novel problem, it needs to collect all of the necessary concepts(knowledge) from long term memory, make multitudes of connections, and test which ones have the highest probability in helping solve the unusual problem. Having followed the above brain modifying steps, once the brain finally finds the correct response to the novel problem, the final response comes as a form of inspiration. For a novice, the first step of acquisition of knowledge structure is the most important. However, as expertise increases, the latter two stages of making connections and selection become more important.

Chronic pain control in patients with rheumatoid arthritis (만성통증 환자의 통증 조절)

  • Eun, Young
    • Journal of muscle and joint health
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    • v.2 no.1
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    • pp.17-40
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    • 1995
  • Rheumatoid arthritis is the one of the chronic diseases, one of its major symptoms is a chronic pain. Despite developing medical treatment and surgical techniques, it is suggested that to control the pain is the goal of the treatment. But pain is an inner experience and even those closest to the patient cannot truly observe its progress or share in its suffering. The National Academy of Sciences Institute of Medicine's report on Pain and Disability concluded that there is no objective measure of pain-(exactly) no pain thermometer-nor can there ever be one, because the experience of pain is inseparable from personal perception and social influence such as culture. To explore chronic pain experience is to understand the process and property of the patient's perception of pain through the response to pain, the coping with pain, and the adaptation to pain. Therefore a qualitative study was conducted in order to gain an understanding of pain experience of patients with RA in korea. I used naturalistic inquiry as a research methodology, which had 5 axioms, the first is that realities are multiple, constructed, and holistic, the second is that knower and known are interactive, inseparable, the third is only time and context bound working hypotheses(idiographic statements) are possible, the forth is all entities are in a state of mutual simultaneous shaping, so that it is impossible to distinguish causes from effects and the last is that inquiry is value-bound. Purposive sampling was conducted as a sampling. 20 subjects who experienced pain over 10 years, lived in middle-sized city and big city in Korea, and 17 women and 3 men. The subject's age was from 32 to 62 (average 48.8), all were married, living with their spouse and children, except two-one divorced and the other widow before they became ill. I collected data using In depth structured interview. I had interviews two or three times with each subject, and the interviews were conducted at each subject's home. Each interview lasted about two hours an average. A recording was taken with the consent of the subject. I used inductive data analysis-such as unitizing and categorizing. unitizing is a process of coding, whereby raw data are systematically transformed and aggregated into units. Categorizing is a process wherby previously unitized data are organized into categories that provide descriptive or inferential information about the context or setting from which the units were derived. This process is used constant comparative method. The pain controlling process is composed of behavior of pain control. The behaviors of pain control are rearranging of ADL, hiddening role conflict, balancing treatment, and changing social relation. Rearranging of ADL includes diet management, sleep management, and the adjustment of daily life activities. The subjects try to rearrange their daily activities by modified style of motions, rearranging time span & range of activities, using auxillary facilities, and getting help in order to keep on the pace of daily life. Hiddening role conflict means to reduce conflicts between sick role and their role as a family member. In this process, the subjects use two modes, one is to control the pain complaints, and the other is to internalize the value which is to stay home is good for caring her children and being a good mother. To control pain complaints is done by 'enduring', 'understanding' the other family members, or making them undersood in order to reduce pain. Balancing treatment is composed of two aspects. One is to keep the pain within the endurable level, the other is to keep in touch with medical personnel in order to get the information of treatment and emotional support. Changing social relation is made by information seeking and sharing, formation of mutual support relation, and finally simplification of social relationships. The subjects simplify their social relationships by refraining from relations with someone who makes them physically and psychologically strained. In particular the subjects are apt to avoid contact with in-laws, and the change of relation to in-laws results in lessening the family boundary. In the course of this process, they confront the crisis of family confict result in family dissolution. This crisis is related to the threat of self-existence. Findings from this study contribute to understanding the chronic pain experience. To advance this study, we should compare this result with other cases in different cultural contexts. I think to interpret these results, korean cultural background should be considered. Especially the different family concept, more broader family members and kinship network, and the traditional medical knowledge influences patients' behavior.

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Organizing an in-class hackathon to correct PDF-to-text conversion errors of Genomics & Informatics 1.0

  • Kim, Sunho;Kim, Royoung;Nam, Hee-Jo;Kim, Ryeo-Gyeong;Ko, Enjin;Kim, Han-Su;Shin, Jihye;Cho, Daeun;Jin, Yurhee;Bae, Soyeon;Jo, Ye Won;Jeong, San Ah;Kim, Yena;Ahn, Seoyeon;Jang, Bomi;Seong, Jiheyon;Lee, Yujin;Seo, Si Eun;Kim, Yujin;Kim, Ha-Jeong;Kim, Hyeji;Sung, Hye-Lynn;Lho, Hyoyoung;Koo, Jaywon;Chu, Jion;Lim, Juwon;Kim, Youngju;Lee, Kyungyeon;Lim, Yuri;Kim, Meongeun;Hwang, Seonjeong;Han, Shinhye;Bae, Sohyeun;Kim, Sua;Yoo, Suhyeon;Seo, Yeonjeong;Shin, Yerim;Kim, Yonsoo;Ko, You-Jung;Baek, Jihee;Hyun, Hyejin;Choi, Hyemin;Oh, Ji-Hye;Kim, Da-Young;Park, Hyun-Seok
    • Genomics & Informatics
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    • v.18 no.3
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    • pp.33.1-33.7
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    • 2020
  • This paper describes a community effort to improve earlier versions of the full-text corpus of Genomics & Informatics by semi-automatically detecting and correcting PDF-to-text conversion errors and optical character recognition errors during the first hackathon of Genomics & Informatics Annotation Hackathon (GIAH) event. Extracting text from multi-column biomedical documents such as Genomics & Informatics is known to be notoriously difficult. The hackathon was piloted as part of a coding competition of the ELTEC College of Engineering at Ewha Womans University in order to enable researchers and students to create or annotate their own versions of the Genomics & Informatics corpus, to gain and create knowledge about corpus linguistics, and simultaneously to acquire tangible and transferable skills. The proposed projects during the hackathon harness an internal database containing different versions of the corpus and annotations.