• Journal of Microbiology and Biotechnology
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• 제26권8호
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• pp.1473-1480
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• 2016

This study focused on the variations in the non-coding sequences between ctxB and rstR of various CTX phages. The non-coding sequences of CTX-1 and CTX-cla are phage type-specific. The length of the non-coding region of CTX-1 and CTX-cla is 601 and 730 nucleotides, respectively. The non-coding sequence of CTX phage could be divided into three regions. There is a phage type-specific Variable region between two homologous Common regions (Common regions 1 and 2). The non-coding sequence of RS1 element is similar to CTX-1 except that Common region 1 is replaced by a short RS1-specific sequence. The non-coding sequences of CTX-2 and CTX-cla are homologous, indicating the non-coding sequence of CTX-2 is derived from CTX-cla. The non-coding region of CTX-O139 is similar to CTX-cla and CTX-2; however, it contains an extra phage type-specific sequence between Common region 2 and rstR. The variations in the non-coding sequences of CTX phages might be associated with the difference in the replication efficiency and the directionality in the integration into the V. cholerae chromosome.

• 대한전자공학회논문지SD
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• 제46권11호
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• pp.24-33
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• 2009

코딩정보의 전송을 위하여 보조선에서 발생하는 오버헤드전이는 Sequence Switching Code (SSC) 알고리즘의 확장성을 제한하는 가장 큰 걸림돌이 되어왔다. 본 논문에서는 보조선을 사용하지 않고 SSC 회로를 구현하는 방법과 함께 이 방법을 사용하여 보조선을 사용하였을 때 보다 오버헤드전이를 적게 발생시키는 방법을 제시하였다. 실험결과 새로운 방법을 적용함으로써 보조선을 사용하는 방식에 비하여 오버헤드전이의 발생을 50% 이하로 줄이고 알고리즘의 효율을 약 30% 향상시킬 수 있었다.

• 한국미생물·생명공학회지
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• 제25권1호
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• pp.23-29
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• 1997

The nucleotide sequence of the estI gene encoding acetylxylan esterase I of Bacillus stearothermophilus was determined and analyzed. The estI gene was found to consist of a 810 base pair open reading frame coding for a polypeptide of 270 amino acids with a deduced molecular weight of 30 kDa. This was in well agreement with the molecular weight (29 kDa) estimated by SDS-PAGE of the purified esterase. The coding sequence was preceded by a putative ribo some binding site 10 bp upsteam of the ATG codon. Further 53 bp upstream, the transcription initiation signals were identified. The putative $_{-}$10 sequence (TCCAAT) and $_{-}$35 seqence (TTGAAT) corresponded closely to the respective consensus sequences for the Bacillus subtiis major RNA polymerase. The G+C content of the coding region of the estI was 51% whereas that of the third position of codone was 60.2%. The N-terminal amino acid sequence of the EstI deduced from the nucleotide sequence perfectly matched the corresponding region of the purified esterase described previously. Comparison with the amino acid sequence of other esterases and lipases reported so far allowed us to identify a sequence, GLSMG at positions 123 to 127 of the EstI which was reported to be the highly conserved active site sequence for those enzymes. The nucleotide sequence of the estI revealed 55.7% homology to that of the xylC coding for the acetylxylan esterase of Caldocellum saccharolyticum.

• 대한전자공학회논문지
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• 제12권2호
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• pp.1-10
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• 1975

본 논문은 사진데이타에 쉽게 적용할수있는 한 adaptive data compression 방법을 노표시하였다. 이웃 sample data 사이의 높은 correlation때문에 발생할 부호화 복잡성을 간편한 sample difference data로 대처하였으며, 자단의 statistical nonstationarity에 적응키 위해서 여덟가지 부호(code)로 구성된 code set중에서 최적부호를 선택토록 하였다. code erst는 두가지 등장부호와 여섯가지 보완형 Shannon-Fanro 부호로 되었다. difference data의 확률분포는 Laplacian model로, entropy의 확률분포는 Gaussian model대 하였다. 부호선별 Paranleter로서 entropy와 Pr[차이값=0]=Po를 비교하였다. 콤퓨타 실험결과 이 adaptive coding 방법으로 2대 1의 데이타 감축비를 얻었다. 이 방법은 fixed coding에 비해서 데이타 감축비와 부호화효율에서 약 10%와 15%의 이득을 주었다. 또한 도는 entropy보다 휠신 편리한 부호선별 parameter인 중시에 entropy 경우와 1% 내외의 좋은 결과를 얻을수 있음이 확인되었다. This paper presents an adaptive data compression algorithm for video data. The coling complexity due to the high correlation in the given data sequence is alleviated by coding the difference data, sequence rather than the data sequence itself. The adaptation to the nonstationary statistics of the data is confined within a code set, which consists of two constant length cades and six modified Shannon·Fano codes. lt is assumed that the probability distributions of tile difference data sequence and of the data entropy are Laplacian and Gaussion, respectively. The adaptive coding performance is compared for two code selection criteria: entropy and pr[difference value=0]=po. It is shown that data compression ratio 2 : 1 is achievable with the adaptive coding. The gain by the adaptive coding over the fixed coding is shown to be about 10% in compression ratio and 15% in code efficiency. In addition, po is found to he not only a convenient criterion for code selection, but also such efficient a parameter as to perform almost like entropy.

• 대한전자공학회논문지SD
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• 제41권7호
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• pp.71-79
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• 2004

패킷형 데이터전송은 다량의 데이터가 연속적으로 전달되는 특성이 있다. 이와 같은 데이터를 버스를 통하여 전송할 패에 데이터의 전송순서는 버스의 전력소모에 영향을 주는 하나의 요소로서 작용한다. 본 논문에서는 패킷형 데이터를 전송할 때 데이터의 전송순서를 변화시켜 버스의 전이횟수를 줄임으로써 버스의 소비전력을 감소시키는 순서변환코딩 (Sequence-Switch Coding, SSC)을 제안하였다. 또한 SSC를 구현하는 알고리즘을 개발하였으며, 실험을 통하여 이들 알고리즘으로 기존의 널리 알려진 Bus-Invert Coding보다 우수한 성능을 얻을 수 있음을 보였다. SSC는 버스의 소비전력을 줄이는 하나의 방법이며, 이를 실현하는 알고리즘은 무수히 많다. 알고리즘의 다양성은 SSC가 갖는 하나의 장점으로써 회로설계자에게 버스의 소비전력과 회로의 구현부담 사이에서 넓은 범위에 걸쳐 절충할 수 있는 자유를 제공해 준다.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제9권1호
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• pp.242-259
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• 2015

Three-dimensional video coding is one of the main challenges restricting the widespread applications of 3D video and free viewpoint video. In this paper, a novel fractal coding algorithm with motion-vector-field-based motion estimation for depth map sequence is proposed. We firstly add pre-search restriction to rule the improper domain blocks out of the matching search process so that the number of blocks involved in the search process can be restricted to a smaller size. Some improvements for motion estimation including initial search point prediction, threshold transition condition and early termination condition are made based on the feature of fractal coding. The motion-vector-field-based adaptive hexagon search algorithm on the basis of center-biased distribution characteristics of depth motion vector is proposed to accelerate the search. Experimental results show that the proposed algorithm can reach optimum levels of quality and save the coding time. The PSNR of synthesized view is increased by 0.56 dB with 36.97% bit rate decrease on average compared with H.264 Full Search. And the depth encoding time is saved by up to 66.47%. Moreover, the proposed fractal depth map sequence codec outperforms the recent alternative codecs by improving the H.264/AVC, especially in much bitrate saving and encoding time reduction.

• 전자공학회논문지B
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• 제30B권7호
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• pp.70-82
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• 1993

In this paper, employing the 3-dimensional discrete cosine transform (DCT) for the utilization of the temporal correlation, an adaptive motion sequence coding technique is proposed. The energy distribution in a 3-D DCT block, due to the nonstationary nature of the image data, varies along the veritical, horizontal and temporal directions. Thus, aiming an adaptive system to local variations, adaptive procedures, such as the 3-D classification, the classified linear scanning technique and the VLC table selection scheme, have been implemented in our approach. Also, a hybrid structure which adaptively combines inter-frame coding is presented, and it is found that the adaptive hybrid frame coding technique shows a significant performance gain for a moving sequence which contains a relatively small moving area. Through an intensive computer simulation, it is demonstrated that, the performance of the proposed 3-D transform coding technique shows a close relation with the temporal variation of the sequence to be code. And the proposed technique has the advantages of skipping the computationally complex motion compensation procedure and improving the performance over the 2-D motion compensated transform coding technique for rates in the range of 0.5 ~ 1.0 bpp.

• 전자공학회논문지CI
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• 제38권6호
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• pp.93-105
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• 2001

격자 벡터 양자화의 비 손실 과정에서는 생성된 코드단어들을 radius 열과 Index 열로 열거한다. radius 열은 run-length 부호화한 한 다음 Entropy 부호화한다. 또한 index 열들은 이진의 고정길이로 표현한다. 비트율이 증가함에 따라 index 비트는 선형적으로 증가하여서 부호화 성능을 감소시킨다. 이 논문에서는, 넓은 비트율의 범위에서 index 비트를 줄이기 위해서, set partitioning 방식을 채택한 새로운 열거 알고리즘을 개발하였다. 제안된 열거 방법은 큰 index 값을 작은 값들을 천이 시켜서 index 비트를 줄인다. 제안된 비손실 기법을 웨이블릿 기반의 영상 부호화에 적용시켰을 때, 0.3 bits/pixel 이상의 비트룰에서 기존의 비손실 부호화 방식보다 10%이상의 비트율을 감소시켰다.

• Journal of Microbiology and Biotechnology
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• 제5권3호
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• pp.117-124
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• 1995

A gene (xynA) encoding the endo-xylanase (E.C.3.2.1.8) from Bacillus stearothermophilus was cloned in E. coli, and its complete nucleotide sequence was determined. The xynA gene consists of a 636 base pairs open reading frame coding for a protein of 212 amino acids with a deduced molecular weight of 23, 283 Da. A putative signal sequence of 27 amino acid residues shows the features comparable with the Bacillus signal sequences; namely, the signal contains a positively charged region close to the N-terminus followed by a long hydrophobic string. The coding sequence is preceded by a possible ribosome binding site with a free energy value of -16.6 kcal/mol and the transcription initiation signals are located further upstream. The translation termination codon (TAA) at the 3 end of the coding sequence is followed by two palindrome sequences, one of which is thought to act as a terminator. The xynA gene has a high GC content, especially in the wobble position of codons (64%). Comparison of the primary protein sequence with those of other xylanases shows a high homology to the xylanases belonging to family G.

• Journal of Animal Science and Technology
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• 제50권2호
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• pp.177-184
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• 2008

Acid-labile subunit(ALS)는 85-kDa 크기의 당단백질로서 7.5-kDa의 insulin-like growth factor(IGF) 및 40~45-kDa IGF-binding protein-3와 결합하여 150-kDa ternary complex를 형성하는 혈장단백질이다. 선행연구에서 본 연구진은 reverse transcription-polymerase chain reaction(RT-PCR) 방법으로 돼지(porcine) ALS(pALS)의 coding sequence를 합성하여 plasmid vector에 삽입시켜 ‘expression construct’를 제작한 바 있다. 그러나 본 expression construct의 pALS coding sequence에는 PCR error로 추정되는 원인으로 말미암아 2개의 bases에서 mis-sense mutation이 일어난 것이 발견되었다. 본 연구에서는 ‘site-directed mutagenesis’ 방법으로 pALS의 올바른 coding sequence를 합성하여 ‘insert DNA’의 마지막 codon 다음에 ‘His-tag’ sequence가 위치한 pET- 28a(+) plasmid expression vector에 삽입하였다. 본 expression construct는 E. coli BL21(DE3) 세포에서 ‘induction’ 시켰고, 발현된 유전자재조합(recombinant) peptide는 Ni-affinity chromato- graphy로 정제하였다. 이렇게 affinity chro- matography로 정제된 peptide는 SDS-PAGE에서 66kDa 위치에 single band를 나타냄으로써 recombinant pALS의 예상된 질량과 일치하였다. 이상의 결과는 본 연구에서 recombinant pALS peptide가 성공적으로 발현정제되었음을 시사한다.