• Title/Summary/Keyword: Coding Diversity

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Genetic Diversity and Clustering of the Rhoptry Associated Protein-1 of Plasmodium knowlesi from Peninsular Malaysia and Malaysian Borneo

  • Ummi Wahidah Azlan;Yee Ling Lau;Mun Yik Fong
    • Parasites, Hosts and Diseases
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    • v.60 no.6
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    • pp.393-400
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    • 2022
  • Human infection with simian malaria Plasmodium knowlesi is a cause for concern in Southeast Asian countries, especially in Malaysia. A previous study on Peninsular Malaysia P. knowlesi rhoptry associated protein-1 (PkRAP1) gene has discovered the existence of dimorphism. In this study, genetic analysis of PkRAP1 in a larger number of P. knowlesi samples from Malaysian Borneo was conducted. The PkRAP1 of these P. knowlesi isolates was PCR-amplified and sequenced. The newly obtained PkRAP1 gene sequences (n=34) were combined with those from the previous study (n=26) and analysed for polymorphism and natural selection. Sequence analysis revealed a higher genetic diversity of PkRAP1 compared to the previous study. Exon II of the gene had higher diversity (π=0.0172) than exon I (π=0.0128). The diversity of the total coding region (π=0.0167) was much higher than those of RAP1 orthologues such as PfRAP-1 (π=0.0041) and PvRAP1 (π=0.00088). Z-test results indicated that the gene was under purifying selection. Phylogenetic tree and haplotype network showed distinct clustering of Peninsular Malaysia and Malaysian Borneo PkRAP1 haplotypes. This geographical-based clustering of PkRAP1 haplotypes provides further evidence of the dimorphism of the gene and possible existence of 2 distinct P. knowlesi lineages in Malaysia.

Genetic Diversity and Characterization of DPE1 Gene in Rice Germplasm

  • Aueangporn Somsri;Yong-Jin Park
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2022.10a
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    • pp.220-220
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    • 2022
  • Disproportionating Enzyme 1 (DPE1) is an a-1,4-D-glucanotransferase that cleavages the a-1,4-glucosidic bonds and transfers glucosyl groups. In rice endosperm, it participates in starch synthesis by transferring maltooligosyl groups from amylose and amylopectin to amylopectin. Here, we investigated the haplotype variations and evolutionary indices (e.g., genetic diversity and population structure) for the DPE1 gene in 374 rice accessions representing seven subgroups (wild, indica, temperate japonica, tropical japonica, aus, aromatic, and admixture). Variant calling analysis of DPE1 coding regions leads to the identification of six functional haplotypes representing/occupying 8 nonsynonymous SNPs. Nucleotide diversity analysis revealed the highest pi-value in wild group (0.0556) compared to other cultivated groups, of which temperate japonica showed the most reduction of genetic diversity value (0.003). A significant positive Tajima's D value (1.6330) of admixture highlights sudden population contraction under balancing selection, while temperate japonica with the lowest Tajima's D value (-1.3523) showed a selection signature of DPE1 domestication which might be the cause of excess of rare alleles. Moreover, these two subpopulations exhibits a greater differentiation (FST=0.0148), indicating a higher genetic diversity. Our findings on functional DPE1 haplotypes will be useful in future breeding programs, and the evolutionary indices can also be applicable in functional studies of the DPE1 gene.

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An Efficient Scheme to Achieve Differential Unitary Space-Time Modulation on MIMO-OFDM Systems

  • Liu, Shou-Yin;Chong, Jong-Wha
    • ETRI Journal
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    • v.26 no.6
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    • pp.565-574
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    • 2004
  • Differential unitary space-time modulation (DUSTM) has emerged as a promising technique to obtain spatial diversity without intractable channel estimation. This paper presents a study of the application of DUSTM on multiple-input multiple-output orthogonal frequency division multiplexing (MIMO-OFDM) systems with frequency-selective fading channels. From the view of a correlation analysis between subcarriers of OFDM, we obtain the maximum achievable diversity of DUSTM on MIMO-OFDM systems. Moreover, an efficient implementation strategy based on subcarrier reconstruction is proposed, which transmits all the signals of one signal matrix in one OFDM transmission and performs differential processing between two adjacent OFDM blocks. The proposed method is capable of obtaining both spatial and multipath diversity while reducing the effect of time variation of channels to a minimum. The performance improvement is confirmed by simulation results.

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Distributed Quasi-Orthogonal Space-Time Block Code for Four Transmit Antennas with Information Exchange Error Mitigation

  • Tseng, Shu-Ming;Wang, Shih-Han
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.7 no.10
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    • pp.2411-2429
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    • 2013
  • In this paper, we extend the case of information exchange error mitigation for the distributed orthogonal space-time block code (DOSTBC) for two transmit antennas to distributed quasi-orthogonal space-time block code (DQOSTBC) for four transmit antennas. A rate 1 full-diversity DQOSTBC for four transmit antennas is designed. The code matrix changes according to different information exchange error cases, so full diversity is maintained even if not all information exchange is correct. We also perform analysis of the pairwise error probability. The performance analysis indicates that the proposed rate 1 DQOSTBC outperforms rate 1/2 DOSTBC for four transmit antennas at the same transmission rate, which is confirmed by the simulation results.

Performance Analysis of Coded Cooperation based Relayed Transmission under Rayleigh Fading Channels

  • Asaduzzaman;Kong, Hyung-Yun
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.36 no.4A
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    • pp.345-355
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    • 2011
  • This paper introduces the coded cooperation protocol in wireless relay network. We propose a two relay based coded cooperation protocol with RCPC codes for wireless networks. The proposed two-relay based system can achieve a diversity of order 4 under slow fading environment. Under fast fading, the diversity order is 2 times of the free distance of the convolutional code. We develop upper bounds on BER and FER for the system under both slow and fast fading with Rayleigh distribution. The effect of various channel conditions on the cooperation is also examined in this work.

Performance Analysis of HDR-WPAN System with Concatenated Space-Time Diversity Scheme (연접 시공간 다이버시티 기법을 적용한 HDR-WPAN 시스템의 성능 분석)

  • Kang, Chul-Gyu;Oh, Chang-Heon
    • Journal of Advanced Navigation Technology
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    • v.11 no.3
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    • pp.288-295
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    • 2007
  • In this paper, we proposed two systems, STTC scheme and STBC-TCM scheme, to enhance the reliability of HDR-WPAN system and analyzed BER(bit error rate) performance of the proposed systems over the slow fading channel. The proposed systems had a diversity gain and coding gain without increasing an additional channel bandwidth. However, in terms of reliability, about 4dB improvement at BER=$10^{-4}$ was obtained by the STBC-TCM scheme. In addition, HDR-WPAN system with STBC-TCM scheme had a linear rise in system complexity of ML(maximum likelihood). From the results, STBC-TCM scheme was more appropriate to improve the reliability and channel efficiency and to reduce complexity of HDR-WPAN system.

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A Y-linked SNP in SRY Gene Differentiates Chinese Indigenous Swamp Buffalo and Introduced River Buffalo

  • Zhang, Yi;Sun, Dongxiao;Yu, Ying;Zhang, Yuan
    • Asian-Australasian Journal of Animal Sciences
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    • v.19 no.9
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    • pp.1240-1244
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    • 2006
  • The complete coding region sequence of the SRY gene in Chinese swamp buffalo was determined by PCR product sequencing. Comparison of swamp and river buffalo SRY gene sequences revealed a single nucleotide polymorphism (SNP, C/G) at the 202 bp site of the coding region. Further, a total of 124 male domestic buffaloes were genotyped at this SNP site using the PCR-SSCP method, and it was found that all Chinese indigenous swamp buffaloes had a guanine (G) at this site, while introduced river buffaloes and crossbred buffaloes showed a cytosine (C). Our findings suggested that this Y-linked SNP displayed type-specific alleles differentiating swamp and river buffaloes, and could be used as an effective marker to detect crossbreeding of swamp buffaloes with introduced river buffaloes in native buffalo populations, and thereby assess genetic diversity status and make proper conservation decisions for indigenous swamp buffaloes. In addition, this SNP can be potentially applied in the study of Asian water buffalo phylogeny from a male perspective.

Genetic Diversity of Barley Cultivars as Revealed by SSR Masker

  • Kim, Hong-Sik;Park, Kwang-Geun;Baek, Seong-Bum;Suh, Sae-Jung;Nam, Jung-Hyun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.5
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    • pp.379-383
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    • 2002
  • Allelic diversity of 44 microsatellite marker loci originated from the coding regions of specific genes or the non-coding regions of barley genome was analyzed for 19 barley genotypes. Multi-allelic variation was observed at the most of marker loci except for HVM13, HVM15, HVM22, and HVM64. The number of different alleles ranged from 2 to 12 with a mean of 4.0 alleles per micro-satellite. Twenty-one alleles derived from 10 marker loci are specific for certain genotypes. The level of polymorphism (Polymorphic Information Content, PIC) based on the band pattern frequencies among genotypes was relatively high at the several loci such as HVM3, HVM5, HVM14, HVM36, HVM62 and HVM67. In the cluster analysis using genetic similarity matrix calculated from microsatellite-derived DNA profiles, two major groups were classified and the spike-row type was a major factor for clustering. Correlation between genetic similarity matrices based on microsatellite markers and pedigree data was highly significant ($r=0.57^{**}$), but these two parameters were moderately associated each other. On the other hand, RAPD-based genetic similarity matrix was more highly associated with microsatellite-based genetic similarity ($r=0.63^{**}$) than coefficient of parentage.

The complete chloroplast genome of Scrophularia kakudensis and a comparative analysis of S. kakudensis and S. cephalantha

  • Ogyeong SON;KyoungSu CHOI
    • Korean Journal of Plant Taxonomy
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    • v.53 no.3
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    • pp.237-241
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    • 2023
  • The genus Scrophularia L. (Scrophulariaceae) comprises 200-270 species worldwide and is a taxonomically challenging lineage, displaying morphological diversity and hybridization. S. kakudensis is morphologically similar to the closely related taxa S. kakudensis var. microphylla, S. pilosa, and S. cephalantha. Therefore, the purpose of this study was to sequence the chloroplast (cp) genome of S. kakudensis using next-generation sequencing and compare it to those of related taxa. The complete cp genome sequence of Scrophularia kakudensis was found to be 152,355 bp long, consisting of a pair of inverted repeats of 25,485 bp that separate a large single-copy (LSC) of 83,479 bp from small single-copy regions of 17,909 bp. The cp genome contained 78 protein-coding genes, 30 tRNAs, and four rRNAs. A phylogenetic analysis based on 78 protein-coding genes from six Scrophularia species showed S. kakudensis and S. cephalantha formed with 100% bootstrap values. We compared the complete cp genomes of S. kakudensis and S. cephalantha and identified seven sequence divergence regions: matK/rps16, rps16/trnQ, trnS/trnG, rpoB/trnC, trnS/trnG, rpl32/trnL, and ndhD/psaC. These regions may be useful for determining the phylogenetic relationships among S. kakudensis-related species.