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http://dx.doi.org/10.5713/ajas.2006.1240

A Y-linked SNP in SRY Gene Differentiates Chinese Indigenous Swamp Buffalo and Introduced River Buffalo  

Zhang, Yi (Department of Animal Genetics & Breeding, College of Animal Science and Technology China Agricultural University)
Sun, Dongxiao (Department of Animal Genetics & Breeding, College of Animal Science and Technology China Agricultural University)
Yu, Ying (Department of Animal Genetics & Breeding, College of Animal Science and Technology China Agricultural University)
Zhang, Yuan (Department of Animal Genetics & Breeding, College of Animal Science and Technology China Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.19, no.9, 2006 , pp. 1240-1244 More about this Journal
Abstract
The complete coding region sequence of the SRY gene in Chinese swamp buffalo was determined by PCR product sequencing. Comparison of swamp and river buffalo SRY gene sequences revealed a single nucleotide polymorphism (SNP, C/G) at the 202 bp site of the coding region. Further, a total of 124 male domestic buffaloes were genotyped at this SNP site using the PCR-SSCP method, and it was found that all Chinese indigenous swamp buffaloes had a guanine (G) at this site, while introduced river buffaloes and crossbred buffaloes showed a cytosine (C). Our findings suggested that this Y-linked SNP displayed type-specific alleles differentiating swamp and river buffaloes, and could be used as an effective marker to detect crossbreeding of swamp buffaloes with introduced river buffaloes in native buffalo populations, and thereby assess genetic diversity status and make proper conservation decisions for indigenous swamp buffaloes. In addition, this SNP can be potentially applied in the study of Asian water buffalo phylogeny from a male perspective.
Keywords
Swamp buffalo; River buffalo; SRY; SNP; Genetic diversity; Conservation;
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1 Barker, J. S. F., S. G. Tan, O. S. Selvarah and T. K. Mukherjee. 1997a. Genetic variation within and relationships among populations of Asian water buffalo (Bubalus bubalis). Anim. Genet. 28:1-13   DOI   ScienceOn
2 Chen, W. and G. Xu. 2004. Photograph Album of China Indigenous Farm Animal Breeds. China Agriculture Press. Beijing
3 Jin, H., Yu-min Zhao and Guo-li Zhou. 2005. Analysis of microsatellite DNA polymorphisms in five China native cattle breeds and application to population genetics studies. Asian- Aust. J. Anim. Sci. 18:1696-1700   DOI
4 Kikkawa, Y., H. Yonekawa, H. Suzuki and T. Amano. 1997. Analysis of genetic diversity of domestic water buffaloes and anoas based on variations in the mitochondrial gene by cytochrome b. Anim. Genet. 28:195-201   DOI   ScienceOn
5 Ma, Y., Y. Chen, W. Feng and D. Wang. 2002. Germplasm of Chinese domestic animals and their conservation. Review of China Agricultural Science and Technology 4:37-42
6 Sun, W., H. Chang, Z. P. Yang, R. Q. Geng, S. X. Lu, G. B. Chang, W. Xu, H. Y. Wang, Z. J. Ren and K. Tsunoda. 2002. Studies on the genetic relationships of sheep populations from east and south of Central Asia. Asian-Aust. J. Anim. Sci. 15:1398-1402   DOI
7 Tanaka, K., C. D. Solis, J. S. Masangkay, K. Maeda, Y. Kawamoto and Y. Namikawa. 1996. Phylogenetic relationship among all living species of the genus Bubalus based on DNA sequences of the cytochrome b gene. Biochem. Genet. 34:443-452   DOI
8 Bradley, D. G., D. E. MacHugh, R. T. Loftus, R. S. Sow, C. H. Hoste and E. P. Cunningham. 1994. Zebu-taurine variation in Y chromosomal DNA: a sensitive assay for genetic introgression in West African tryanotolerant cattle populations. Anim. Genet. 15:7-12
9 Mason, I. L. 1974. Special, types and breeds. In The Husbandry and Health of the Domestic Buffalo (Ed. W. R. Cockrill). FAO, Rome. pp. 1-47
10 Fischer, H. and F. Ulbrich. 1968. Chromosomes of the Murrah buffalo and its crossbreds with the Asiatic Swamp buffalo (Bubalus bubalis). Zeitschrift fur Tierzuchtung und Zuchtungsbiologie 84:110-114
11 Parma, P., M. Feligini, G. Greppi and G. Enne. 2004. The complete coding region sequence of River buffalo (Bubalus bubalis) SRY gene. DNA sequence 15:77-80   DOI
12 Lau, C. H., R. D. Drinkwater, K. Yusoff, S. G. Tan, D. J. Hetzel and J. S. F. Barker. 1998. Genetic diversity of Asian water buffalo (Bubalus bubalis): mitochondrial DNA D-loop and cytochrome b sequence variation. Anim. Genet. 29:253-264   DOI   ScienceOn
13 Barker, J. S. F., S. S. Moore, D. J. S. Hetzel, D. Evans, S. G. Tan and K. Byrne. 1997b. Genetic diversity of Asian water buffalo (Bubalus bubalis): microsatellite variation and a comparison with protein-coding loci. Anim. Genet. 28:103-115   DOI   ScienceOn
14 Kikkawa, Y., T. Takada, K. Sutopo, K. Nomura, T. Namikawa, H. Yonekawa and T. Amano. 2003. Phylogenies using mtDNA and SRY provide evidence for male-mediated introgression in Asian domestic cattle. Anim. Genet. 34:96-101   DOI   ScienceOn
15 Kumar, S., K. Tamura and M. Nei. 2004. MEGA3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Briefings in Bioinformatics 5:150-163   DOI   ScienceOn
16 Tanaka, K., T. Yamagata, J. S. Masangkay, M. O. Faruque, D. Vu- Binh, Salundik, S. S. Mansjoer, Y. Kawamoto and T. Namikawa. 1995. Nucleotide diversity of mitochondrial DNAs between the Swamp and the River types of domestic water buffaloes, Bubalus bubalis, based on restriction endonuclease cleavage patterns. Biochem. Genet. 33:137-148   DOI
17 Amano, T., Y. Miyakoshi, T. Takada, Y. Kikkawa and H. Suzuki. 1994. Genetic variants of ribosomal DNA and mitochondrial DNA between Swamp and River buffaloes. Anim. Genet. 25(Supplement 1):29-36   DOI
18 Tu, Y. J., K. W. Chen, S. J. Zhang, Q. P. Tang, Y. S. Gao and N. Yang. 2006. Genetic diversity of 14 indigenous grey goose breeds in China based on microsatellite markers. Asian-Aust. J. Anim. Sci. 19:1-6
19 Edwards, C. J., C. Gaillard, D. G. Bradley and D. E. MacHugh. 2000. Y-specific microsatellite polymorphisms in a range of bovid species. Anim. Genet. 31:127-130   DOI   ScienceOn
20 Kierstein, G., M. Vallinoto, A. Silva, P. S. Schneider, L. Iannuzzi and B. Brenig. 2004. Analysis of mitochondrial D-loop region casts new light on domestic water buffalo (Bubalus bubalis) phylogeny. Molec. Phylogenet. Evol. 30:308-324   DOI   ScienceOn
21 Qu, L., X. Li, G. Wu and N. Yang. 2005. Efficient and sensitive method of DNA silver staining in polyacrylamide gels. Electrop. 26:99-101   DOI   ScienceOn
22 Amano, T. 1983. Genetic differences between Swamp and River buffaloes in biochemical and immunological characteristics. In Proceedings of the Preconference Symposium of the 5th World Conference on Animal Production, Tsukuba, Japan, pp. 131-135
23 FAO Statistical Databases. http://faostat.fao.org/
24 Li, X., K. Li, B. Fan, Y. Gong, S. Zhao, Z. Peng and B. Liu. 2000. The genetic diversity of seven pig breeds in China, estimated by means of microsatellites. Asian-Aust. J. Anim. Sci. 13:1193-1195   DOI
25 Wang, X., H. H. Cao, S. M. Geng and H. B. Li. 2004. Genetic diversity of 10 indigenous pig breeds in China by using microsatellite markers. Asian-Aust. J. Anim. Sci. 17:1219-1222   DOI
26 Hanotte, O., C. L. Tawah, D. G. Bradley, M. Okomo, Y. Verjee, J. Ochieng and J. E. Rege. 2000. Geographic distribution and frequency of a taurine Bos taurus and an indicine Bos indicus Y specific allele amongst sub-Saharan African cattle breeds. Molec. Ecol. 9:387-396   DOI   ScienceOn
27 Sambrook, J., E. F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York, NY