• Title/Summary/Keyword: Cocktail

Search Result 184, Processing Time 0.032 seconds

Use of Flour-Impregnated Polysulfone Membranes for Measuring Radioactive Contamination in Laboratories (실험실 방사성핵종 오염도 측정에 있어 Fluor 함침 폴리설폰 막의 이용)

  • 한명진
    • Membrane Journal
    • /
    • v.9 no.2
    • /
    • pp.132-139
    • /
    • 1999
  • Solid scintillation proximity membranes were prepared for measuring the amount of radioactivity in laboratories contaminated by the radionuclide of $^3H$-cortisol. The membranes, consisting of polysulfone as a polymer matrix and cerium activated yttrium silicate as a fluor, were used to monitor the amount of radioactivity without the aid of a scintillation cocktail required for the conventional wipe test. The test results of the cocktail-free wipe test showed that the prepared membranes were efficient to monitor radionuclide-contaminated areas with the good counting ability as well as with the decrease of overall production of radioactive waste. On the other hand, solvent treatment of the prepared membranes could induce a significant variation of membrane morphology, but the counting efficiency of the solvent-treated membranes was not improved than that of the untreated one.

  • PDF

Optimization of the Bacteriophage Cocktail for the Prevention of Brown Blotch Disease Caused by Pseudomonas tolaasii

  • Yun, Yeong-Bae;Um, Yurry;Kim, Young-Kee
    • The Plant Pathology Journal
    • /
    • v.38 no.5
    • /
    • pp.472-481
    • /
    • 2022
  • Brown blotch disease, caused by Pseudomonas tolaasii, is one of the most serious diseases in mushroom cultivation, and its control remains an important issue. This study isolated and evaluated pathogen-specific bacteriophages for the biological control of the disease. In previous studies, 23 varieties of P. tolaasii were isolated from infected mushrooms with disease symptoms and classified into three subtypes, Ptα, Ptβ, and Ptγ, based on their 16S rRNA gene sequences analysis and pathogenic characters. In this study, 42 virulent bacteriophages were isolated against these pathogens and tested for their host range. Some phages could lyse more than two pathogens only within the corresponding subtype, and no phage exhibited a wide host range across different pathogen subtypes. To eliminate all pathogens of the Ptα, Ptβ, and Ptγ subtype, corresponding phages of one, six, and one strains were required, respectively. These phages were able to suppress the disease completely, as confirmed by the field-scale on-farm cultivation experiments. These results suggested that a cocktail of these eight phages is sufficient to control the disease induced by all 23 P. tolaasii pathogens. Additionally, the antibacterial effect of this phage cocktail persisted in the second cycle of mushroom growth on the cultivation bed.

Fire Test of Fiber Cocktail Reinforced High Strength Concrete Columns without Loading (섬유혼입공법을 적용한 고강도콘크리트 기둥의 비재하 내화시험)

  • Youm, Kwang-Soo;Jeon, Hun-Kyu;Kim, Heung-Youl
    • Journal of the Korea Concrete Institute
    • /
    • v.21 no.4
    • /
    • pp.465-471
    • /
    • 2009
  • To prevent the explosive spalling of the high strength concrete and control the rise of temperature in the steel rebar during fire, a fiber cocktail method has been proposed simultaneously with the use of polypropylene and steel fiber. After applying the fiber cocktail (polypropylene and steel fibers) into the mixture of high strength concrete with a compressive strength of between 40 and 100 MPa and evaluating the thermal properties at elevated temperatures, the fire test was carried out on structural members in order to evaluate the fire resistance performance. Two column specimens were exposed to the fire without loading for 180 minutes based on the standard curve of ISO-834. No explosive spalling has been observed and the original color of specimen surface was changed to light pinkish grey. The inner temperature of concrete dropped rapidly starting from 60mm deep. After 60 minutes of exposure to the fire, the temperature gradient of fiber cocktail reinforced high strength concrete was measured as 2.2oC/mm, which is approximately 5 times less than that of normal concrete. The final temperatures of steel rebar after 180 minutes of fire test resulted in 488.0oC for corner rebar, 350.9oC for center rebar, and 419.5oC for total mean of steel rebar. The difference of mean temperature between corner and center rebar was 137.1oC The tendency of temperature rise in concrete and steel rebar changed between 100oC and 150oC The cause of decrease in temperature rise was due to the water vaporization in concrete, the lower temperature gradient of the concrete with steel and polypropylene fiber cocktails, the moisture movement toward steel rebars and the moisture clogging.

An Experimental Study on The Fire Resistance Performance and Spalling of 100 MPa HSC Column Mixed Fiber-Cocktail (Fiber-Cocktail 섬유를 혼입한 100 MPa 고강도 콘크리트의 단면크기에 따른 폭렬 및 내화성능에 관한 실험적 연구)

  • Kim, Hyung-Jun;Kim, Heung-Yonl;Park, Kyung-Hoon;Yeo, In-Hwan;Kwon, Ki-Hyuck
    • Fire Science and Engineering
    • /
    • v.25 no.5
    • /
    • pp.85-92
    • /
    • 2011
  • Along the fire resisting capacity of high strength concrete has been brought up as a social issue, and the Ministry of Land also had notified the control standard about it, the researches for improving the fire resisting capacity have been considerably activated these days. In this study, we performed a research for developing a high strength concrete, contains the fiber cocktail, which is a hybrid of polypropylene fiber as organic matter, and steel fiber. As we analyzed the temperature of the steel part during the fire test with 100 MPa high strength concrete, there was a tendency that the lower temperature comes out with the larger cross section, and the $600{\times}600mm$, $800{\times}800mm$ cross sectioned can secure the fire resistance capability, so the $600{\times}600mm$ is deducted as the optimal size if we consider the double economic feasibility. As well, among them the best qualified $600{\times}600mm$ shapes, the fiber cocktail hybrid of $1.5kg/m^3$ PP fiber and $40kg/m^3$ of steel, comes out the best ratio.

Isolation of bacteriophages having depolymerase and control of pathogenic E. coli O103 in biofilm on lettuce

  • Park, Dasom;Park, Jong-Hyun
    • Korean Journal of Food Science and Technology
    • /
    • v.51 no.6
    • /
    • pp.604-609
    • /
    • 2019
  • To control pathogenic E. coli in biofilm, bacteriophages were isolated from environmental samples. Seventeen isolates had depolymerase activities by translucent zones at the rims of plaques. To determine biofilm-forming ability, an abiotic plastic surface of polystyrene was used; E. coli O103 showed the highest biofilm formation at 30℃ after 24 h. Moreover, biofilm by E. coli O103 on the biotic surface of lettuce was observed by a scanning electron microscope. The bacteriophage cocktail of ΦNOECP40 and ΦNOECP44 showing depolymerase activities was prepared to eliminate the E. coli inbiofilm. By organic acids, reduction of E. coli in biofilm was insignificant and almost undetectable. However, the abundance of E. coli in biofilm was reduced by 3 log CFU/mL from 7.3 log CFU/mL after 60 min with the bacteriophage cocktail. Therefore, we suggest that bacteriophages with depolymerase could be utilized to effectively control pathogenic E. coli in biofilm.

Development of Dress Design using Infra-Syndrome - Focusing on Design Concept of Lingeries - (인프라 현상을 활용한 드레스 디자인 개발 - 화운데이션 모티브를 중심으로 -)

  • 최은희;송미령
    • The Research Journal of the Costume Culture
    • /
    • v.9 no.4
    • /
    • pp.549-561
    • /
    • 2001
  • Modern fashion can be expressed as 'pursuit of cocktail effect', which means not unformed, not bounded by a rule, and mixed with various styles. Among them, the most outstanding trend of style is the'infra-syndrome'which designs underclothes like a ordinary wear, which has outstood since early 1980s. In 1990s, this syndrome made active progress and now facing 21th century, it becomes one of the big trends in fashion. Clothes of infra syndrome are one of attempts for freedom expressed by progressive designers who pursuit new and surrealistic design. The purpose of this study is to maximize the aesthetic beauty of'infra-syndrome'apparel centering on dresses of which designs are notable derived from underclothes. With the sewing techniques using the special facilities of the lingerie-manufacturing industry and making use of new material which can be used far lingerie wear, this research strives to expand new ideas in the lingerie industry as well as to contribute to promoting the dress culture by developing novel lines from a new form of dress and lingerie-foundations of dress suitable for human body.

  • PDF

Cocktail Making Movie based on User (사용자 기반 Cocktail Making 동영상 제작)

  • Lee, Ji-Hye;Lee, Ju-Young;Oh, Roa;Yoon, Yong-Ik
    • Proceedings of the Korea Information Processing Society Conference
    • /
    • 2007.05a
    • /
    • pp.142-144
    • /
    • 2007
  • 최근 인터넷 상에서는 UCC 붐이 일고 있다. PC상이 아닌 모바일 상에서의 UCC를 지향하며 모바일을 통해 칵테일 관련 동영상을 재생시키는 기술에 대하여 연구하였다. 칵테일에 대한 동영상을 직접 촬영하였으며 동영상 포맷을 모바일 상에서 적합한 형식으로 변환하는 과정을 겪었다. 다운로드 서버를 설계해 모바일 상에서 우리 팀이 촬영한 UCC 동영상을 다운로드 받을 수 있게 시스템을 구현하였다.

Studies on the HACCP Model of Steak-Set Menu in Hotel (HACCP 모델 적용시 호텔 .레스토랑의 세트 메뉴 중 스테이크에 관한 연구)

  • 양신철
    • Journal of Applied Tourism Food and Beverage Management and Research
    • /
    • v.12 no.1
    • /
    • pp.5-24
    • /
    • 2001
  • This study was focused on the sanitary analysis of hazard factors and the establishment of critical control points on steak-set menu In hotel by the documents and microbiological investigation. The hazard factors of shrimp cocktail were microbial contamination, residual pesticides, unsuitable healing and cross contamination. The hazard factors of potato soap were residual pesticides, microorganisms contamination, unsuitable heating and solanine in potato. The hazard factors of simple salad were microorganisms contamination, unsuitable heating and cross contamination by inappropriate package. The hazard factors of steal were residual antimicrobial drugs, microorganisms contamination, unsuitable heating and cross contamination. The critical control points of shrimp cocktail were temperature control , number of washing and center temperature control of heating step. The critical control points of potato soup were stock temperature control , number of washing and center temperature control of Heating step. The critical control points of simple salad were number of washing and dryness of utensil. The critical control points of steak were stock temperature control , number of washing, center temperature and time control of heating step.

  • PDF

Effect of UV-C Irradiation on the Inactivation of Listeria monocytogenes and Lipid Oxidation in Hamburger Patties during Storage (UV-C 조사가 햄버거 패티 저장 중 Listeria monocytogenes의 생육저해 및 지질산화에 미치는 영향)

  • Kim, Hyun-Jin;Kim, Seul-Ki;Chun, Ho-Hyun;Song, Kyung-Bin
    • Food Science and Preservation
    • /
    • v.17 no.3
    • /
    • pp.425-429
    • /
    • 2010
  • Inactivation by UV-C irradiation of Listeria monocytogenes cocktail inoculated on hamburger patties was examined. Hamburger patty samples were inoculated with 6-7 log CFU/mL of L. monocytogenes cocktail, and then exposed to doses of 0, 1, 5, or $10kJ/m^2$ of UV-C light, followed by storage at $4{\pm}1^{\circ}C$ for 7 d. Microbiological evaluation indicated that the populations of L. monocytogenes decreased significantly (p<0.05) as irradiation dose increased. In particular, L. monocytogenes populations decreased by 2.03 log CFU/g after exposure to 10 $kJ/m^2$, compared with control samples. The thiobarbituric acid-reactive substance levels of hamburger patty samples increased during storage, regardless of UV-C irradiation status. These results indicate that UV-C irradiation may be useful in improving the microbial safety of hamburger patties during storage.

Humanized Mice for the Evaluation of Francisella tularensis Vaccine Candidates

  • Oh, Hanseul;Kim, C-Yoon;Kim, Chang-Hwan;Hur, Gyeung-Haeng;Lee, Ji Min;Chang, Seo-Na;Park, Jae-Hak
    • Journal of Microbiology and Biotechnology
    • /
    • v.28 no.1
    • /
    • pp.157-164
    • /
    • 2018
  • Francisella tularensis (FT), a highly infectious pathogen, is considered to be a potential biological weapon owing to the current lack of a human vaccine against it. Tul4 and FopA, both outer membrane proteins of FT, play an important role in the bacterium's immunogenicity. In the present study, we evaluated the immune response of mice - humanized with human CD34+ cells (hu-mice) - to a cocktail of recombinant Tul4 and FopA (rTul4 and rFopA), which were codon-optimized and expressed in Escherichia coli. Not only did the cocktail-immunized hu-mice produce a significant human immunoglobulin response, they also exhibited prolonged survival against an attenuated live vaccine strain as well as human T cells in the spleen. These results suggest that the cocktail of rTul4 and rFopA had successfully induced an immune response in the hu-mice, demonstrating the potential of this mouse model for use in the evaluation of FT vaccine candidates.