• 한국미생물·생명공학회지
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• 제50권4호
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• pp.512-521
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• 2022

Since the main symptoms of COVID-19 involve the respiratory system, the infection rate of this disease is predicted to be higher in patients with other respiratory conditions such as allergic rhinitis. In such a situation, it will be meaningful to conduct research on an allergy treatment that has fewer side effects and can effectively reduce allergy symptoms. Here, we prepared experimental samples under various fermentation conditions with mixed extracts of six medicinal plants. To examine the anti-allergic efficacy of these samples, an egg albumin-induced allergic rhinitis animal model experiment, a serum histamine and IgE experiment, and a COX and LO inhibitory activity experiment were conducted. As a result of animal experiments, OVA+SP-4 showed superior efficacy compared to OVA+SP-1 in nasal rubbing and sneezing experiments and had anti-allergic efficacy similar to that of OVA-cetirizine. The serum histamine concentration of OVA+SP-4 was also 1.3 times higher than that of the OVA+cetirizine group, showing a high histamine reduction ability, and IgE showed the same trend. An analysis of COX inhibitory efficacy also confirmed that COX-1 and COX-2 inhibitory efficacy is high, and the longer the fermentation time, the higher the antiallergic efficacy. The composition proposed by this study is expected to have a significant effect on sustainable allergy prevention and treatment in the future by applying it to human patients.

• 한국미생물·생명공학회지
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• 제51권2호
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• pp.174-183
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• 2023

Various skin diseases are occurring due to external factors such as urbanization and increase in environmental pollution and wearing masks due to COVID-19. Accordingly, various functional cosmetics are being released, but as some side effects are reported, research on functional cosmetics materials using natural plants is necessary. Therefore, in this study, the antioxidant, antibacterial and anti-inflammatory effects of Phyllanthus emblica, Geranium (Pelargonium graveolens), and Commiphora myrrha mixed extracts (PGC) that pharmacological efficacy has been verified were analyzed and their potential as functional cosmetics raw materials was examined. Four extracts (PGC-1~4) were prepared according to the extraction method. ABTS and DPPH radical scavenging activity experiments were conducted for the antioxidant efficacy of the extracts. In addition, paper disc experiments and LPS inflammation-inducing cytokine experiments were conducted to examine the antibacterial and anti-inflammatory effects. In addition, a cell viability test was performed to confirm cytotoxicity. As results of the study, all extracts showed antioxidant, antibacterial, and anti-inflammatory effects without cytotoxicity, and in particular, PGC-4, a fermentation and ultrasonic extract, showed the best efficacy. This means that the extraction yield of useful components varies depending on the extraction method.

• 생물환경조절학회지
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• 제29권3호
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• pp.219-230
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• 2020

본 연구는 시설 딸기(Fragaria × ananassa Duch. cv. Maehyang)의 삽목묘 생산을 위해 적절한 배지 및 가습 기간을 구명하기 위해 수행되었다. 2019년 2월에 코이어(CO), 암면(RW), 페놀폼(PF), 유기성형배지(OFM)에 삽수를 삽목하여 0, 3, 6, 9, 12일간 포깅 처리를 하였으며, 2019년 6월에 현장실증 실험으로 코이어(CO), 암면(RW), 페놀폼(PF), 유기성형배지(OFM)에 삽수를 삽목하여 0, 6, 9, 12, 15일간 미스팅 처리를 하였다. 포깅 처리에서 삽목묘의 발근율과 생존율은 포깅 기간이 길어질수록 증가하는 경향을 보였으며, CO 배지에서 9일 이상 포깅 처리하였을 때 발근율과 생존율이 유의적으로 높았다. 미스팅 처리에서 발근율은 CO와 RW 배지에서 9일 이상 미스팅 처리하였을 때, 생존율은 12일 이상 미스팅 처리하였을 때 유의적으로 높았다. 육묘기에 배지 및 가습 처리에 따른 지상부와 지하부의 생육은 유의적인 차이가 없었다. 따라서 발근율과 생존율을 고려하였을 때, CO 배지에서 9일간 가습 처리하는 것이 안정적인 삽목묘의 생산에 유리할 것으로 판단된다.

• Toxicological Research
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• 제29권1호
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• pp.21-25
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• 2013

The selective targeting of an integrin ${\alpha}_v{\beta}_3$ receptor using radioligands may enable the assessment of angiogenesis and integrin ${\alpha}_v{\beta}_3$ receptor status in tumors. The aim of this research was to label a peptidomimetic integrin ${\alpha}_v{\beta}_3$ antagonist (PIA) with $^{99m}Tc(CO)_3$ and to test its receptor targeting properties in nude mice bearing receptor-positive tumors. PIA was reacted with tris-succinimidyl aminotriacetate (TSAT) (20 mM) as a PIA per TSAT. The product, PIA-aminodiacetic acid (ADA), was radiolabeled with $[^{99m}Tc(CO)_3(H_2O)_3]^{+1}$, and purified sequentially on a Sep-Pak C-18 cartridge followed by a Sep-Pak QMA anion exchange cartridge. Using gradient C-18 reverse-phase HPLC, the radiochemical purity of $^{99m}Tc(CO)_3$-ADA-PIA (retention time, 10.5 min) was confirmed to be > 95%. Biodistribution analysis was performed in nude mice (n = 5 per time point) bearing receptor-positive M21 human melanoma xenografts. The mice were administered $^{99m}Tc(CO)_3$-ADA-PIA intravenously. The animals were euthanized at 0.33, 1, and 2 hr after injection for the biodistribution study. A separate group of mice were also co-injected with 200 ${\mu}g$ of PIA and euthanized at 1 hr to quantify tumor uptake. $^{99m}Tc(CO)_3$-ADA-PIA was stable in phosphate buffer for 21 hr, but at 3 and 6 hr, 7.9 and 11.5% of the radioactivity was lost as histidine, respectively. In tumor bearing mice, $^{99m}Tc(CO)_3$-ADA-PIA accumulated rapidly in a receptor-positive tumor with a peak uptake at 20 min, and rapid clearance from blood occurring primarily through the hepatobiliary system. At 20 min, the tumor-to-blood ratio was 1.8. At 1 hr, the tumor uptake was 0.47% injected dose (ID)/g, but decreased to 0.12% ID/g when co-injected with an excess amount of PIA, indicating that accumulation was receptor mediated. These results demonstrate successful $^{99m}TC$ labeling of a peptidomimetic integrin antagonist that accumulated in a tumor via receptor-specific binding. However, tumor uptake was very low because of low blood concentrations that likely resulted from rapid uptake of the agent into the hepatobiliary system. This study suggests that for $^{99m}Tc(CO)_3$-ADA-PIA to be useful as a tumor detection agent, it will be necessary to improve receptor binding affinity and increase the hydrophilicity of the product to minimize rapid hepatobiliary uptake.

• Korean Chemical Engineering Research
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• 제60권4호
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• pp.606-612
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• 2022

실내 거주 시간이 늘어나면서 발생하는 CO₂를 인체에 무해한 농도인 1,000 ppmCO₂ 이하로 유지하기 위해 연구들이 활발히 진행 중이다. 본 연구에서는 저농도 CO₂흡착제로서 KOH와 K₂CO₃와 같은 알칼리성 첨가제를 상용 활성탄에 함침하여 사용하였다. 흡착된 CO₂ 양은열중량분석기(TGA)와 chamber(CO₂ IR analyzer)로평가하였다. 비표면적이 928.5 m²/g인 상용 활성탄(AC)은 KOH가 함침 된 KOH/AC(13.6 m²/g)와 K₂CO₃가 함침 된 K₂CO₃/AC(288.8 m²/g)보다 비표면적이 높았다. 챔버실험결과, AC는 CO₂를 거의 흡착하지 않았지만, KOH/AC와 K₂CO₃/AC는 각각 93.5 mg_CO2/g_sample 및 94.5 mg_CO2/g_sample 흡착하였다. 이것은 비표면적 및 미세기공의 부피에 의한 물리적인 흡착 영향보다 알칼리성 활성점의 증가가 CO₂ 흡착에 더 유리하게 작용한 것으로 판단된다. KOH/AC와 K₂CO₃/AC의 재생성능은 chamber test 결과 대조군(K₂CO₃/Al+Si supports)과 비교했을 때 안정적으로 흡착 성능을 유지하는 것으로 나타났다(3회 반복 실험). 또한, KOH/AC와 K₂CO₃/AC는열중량분석기의절대습도 1%H₂O를고려한조건에서 145.7 mg_CO2/g_sample및 150 mg_CO2/g_sample로 나타났다. 따라서 KOH 및 K₂CO₃ 등과 같은 알칼리 성분의 함침은 상용 활성탄의 안정적인 흡착 및 재생 후 흡착성능을 나타내어, 실내 이산화탄소 저감을 위한 흡착제 개발에 적용될 수 있을 것으로 판단된다.

• 한국재료학회지
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• 제14권3호
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• pp.175-180
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• 2004

SiC nanotubes were synthesized by CNT-confined reaction. Evaporated SiO gas reacted with carbon nanotubes by VS growth mechanism. By confineded reaction, carbon nanotube was changed to SiC nanotube, and synthesized SiC nanotube was filled partly by the gas reaction in the nanotubes. SiC nanotube's mean diameters were not changed than carbon nanotubes because of means ratio of $CO_2$ and SiO gas was maintained evenly during the process. This result was same of data of simulation. By TEM observastion, SiC nanotube was filled by reaction of inner wall of CNT and SiO gas through the VS reactions. Converted SiC nanotube's compositions were revealed Si and C of 1: 1 ratios at all sites of nanotube by EDS.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XIII)
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• pp.644-647
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• 2003

Materials currently used as an injectable bulking agent in the dermatologic and urologic fields revealed several drawbacks such as particles migration, inflammatory reaction, allergic reaction, rapid volume shrinkage, and necessity of a donor site. In this study, we have developed injectable biomaterial comprising poly (DL- lactide-co-glycolide)(PLGA) and hyaluronic acid gel to overcome these problems. PLGA is a biocompatible synthetic material and hyaluronic acid is a common substance found in living organisms. We examined the feasibility of injection through needle and tested biocompatibility in animal model. After transplantation, injected sites and distant organs were examined histologically to verify a new tissue formation, inflammation, and particles migrations. Injected volume was maintained approximately 80 percent for 2 months. Results demonstrated that the developed material was injectable through various gauges of needles and induced a new bulking tissue formation without serious inflammatory reaction.

• 한국유체기계학회 논문집
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• 제16권5호
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• pp.11-17
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• 2013

This study is aimed to construct a marine small hydro power plant using discharge water of fish farm in Jeju Haengwon-ri. The difference of design methods between marine small hydro power plant and land small hydro power plant is to consider the tides. Moreover, ground condition should be examined because gushout sea water comes out from the ground at high tide in Jeju as the ground of Jeju beach consists of basalt stone. From the field test of the turbine generator after construction of the power plant, output power and efficiency of the turbine generator shows good conformance to the required conditions.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2003년도 생물공학의 동향(XII)
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• pp.591-594
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• 2003

기존 재료들의 단점인 재료의 전이성, 염증 유발, 효과의 단기 지속 등의 문제를 해결하기 위하여 PLGA 미립구를 주 재료로 하는 주사 충전재를 개발하였다. 생쥐 실험 결과 염증 반응이 적고 장기 이동성이 없으며 부피 유지 효과가 우수하다는 결론을 내렸으며 추가적으로 장기 추가 보완 실험을 실시하여 비뇨기과, 성형외과적 주사용 충전재로써의 적합성을 조사하겠다.

• 전력전자학회:학술대회논문집
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• 전력전자학회 2008년도 하계학술대회 논문집
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• pp.624-627
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• 2008

It is generally used the boost converter for the welding machine using fuel cell. But this type generates the duplicate loss between boost converter and inverter. As a result, the whole efficiency is lower than conventional inverter arc welder and the cost is expensive due to additional switching device. Therefore, we proposed the inverter rectification type DC-DC converter with boost converter function in fuel cell based TIG welder. The performance of the proposed technique is evaluated on a 1.2kW fuel cell stack based experimental prototype circuit.