• Title/Summary/Keyword: Co-regeneration system

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Recognition of Stakeholders Regarding Building-Related Landscape Systems (대지의 조경 제도에 대한 관련 주체의 인식 비교 분석)

  • Kim, Yong-Gook;Lee, Sang-Min
    • Journal of the Korean Institute of Landscape Architecture
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    • v.46 no.3
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    • pp.79-91
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    • 2018
  • This study proposes the difference of awareness and improvement plan of building-related landscape systems through a cognition survey of building owners, public officials, and experts. The main results are as follows. First, all three groups are highly aware of the need to secure urban green spaces. Even if private property rights are restricted, the level of awareness that green space should be secured for public benefit is high. The level of interest in the building-related landscape system was also surveyed to a greater degree. Second, the survey respondents, including the building owner group, answered that there is a need to strengthen the building-related landscape area standards. It can be seen that there is a need to revise the relaxation of the building-related landscape area standards through an amendment of the Building Ordinance in many local governments. Third, most municipalities are not equipped with an organizational system that can promote the work of building-related landscape. It is necessary to newly hire civil servants for landscape design, maintenance, and management specialization, or to expand co-working relationships with related departments. Fourth, building owners are interested in building-related landscape, but they do not know specific management methods and leave the landscape space. The municipality needs to offer guidance on landscape architectural design and construction methods that the building owners can easily follow and to support the voluntary landscape space management on the part of the local residents by supporting the trees. Fifth, in order to improve the building-related landscape system, it is necessary to verify the effect of the building-related landscape and spread a consensus. At the government level, in order to enable building owners to recognize the value of the landscape space in connection with an urban regeneration project, it is necessary to present an example of an excellent building-related landscape installation via a pilot project.

Development of transgenic strawberry plants expressing monellin, a sweet protein (감미단백질 모넬린 발현 딸기 형질전환 식물체 개발)

  • Min, Sung Ran;Ko, Suk Min;Lyu, Jae Il;Park, Ji Hyun;Yi, So Young;Lee, In-Ha;Kim, Hyun Sook;Kim, Tae Il;Choi, Pil Son;Jeong, Won-Joong;Kim, Suk Weon;Kim, Jonghyun;Liu, Jang R.
    • Journal of Plant Biotechnology
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    • v.42 no.3
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    • pp.180-185
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    • 2015
  • Leaf discs from 'Yeobong' and 'Maehyang' strawberry plants were used as explants for transformation. The Agrobacterium tumefaciens strain EHA105 harboring the monellin gene under the control of the CaMV 35S promoter was used in co-cultivation experiments. The frequencies of callus formation and plant regeneration from leaf explants after co-cultivation in 'Yeobong' were higher than those of 'Maehyang'. These transgenic plants showed normal growth patterns and flowering. PCR and Southern hybridization confirmed that 1 to 2 copies of the monellin gene were integrated into genome of the transgenic strawberry plants. Northern blot analysis confirm that the transcripts were expressed in transgenic strawberry plants. Although long-term subcultured transgenic strawberry plants showed a phenomenon to escape the transgene, the transformation system established in this study provides new opportunities for genetic improvement of strawberry plants.

Factors Influencing Efficient Agrobacterium-mediated Transformation of Panicum spp. (Agrobacterium법에 의한 Panicum속 식물들의 효과적인 형질전환에 영향을 미치는 요인)

  • Seo, Mi-Suk;Takahara, Manabu;Takamizo, Tadashi
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.31 no.1
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    • pp.1-8
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    • 2011
  • Molecular techniques such as genetic transformation are powerful tools that can be used for the genetic modification of warm-season grasses. The P. meyerianum with high regeneration ability was used for establishing an Agrobacterium-mediated transformation system. We investigated various factors affecting Agrobacterium infection by examining GUS gene expression of pCAMBIA1304 vector. Among various concentration of acetosyringone and betaine tested for inoculation and co-cultivation, 10 mg/L acetosyringone and 60 mg/L betaine resulted in the highest transformation frequency in terms of GUS expression. The calli of 4 species of Panicum spp. with excellent tissue culture response were inoculated with Agrobacterium under the optimal infection conditions. The high activity of GUS gene was observed in all species and hygromycin-resistant calli expressing GFP were obtained in P. meyerianum, P. longijubatum, P. stapfianum and guineagrass Noh-PL1. Co-cultivated calli were transferred onto the selection medium containing hygromycin, and the hygromycin resistant calli were selected after 3 months. Hygromycin-resistant plantlets were then successfully regenerated from the calli and grown in a greenhouse. We confirmed stable insertion of hpt gene among the hygromycin-resistant plantlets of P. meyerianum by PCR analysis.

Comparison between Poly(lactic-co-glycolic acid) Films Contained Natural Polymers on Adhesion and Proliferation of Schwann Cells (천연 고분자가 함유된 락타이드 글리콜라이드 공중합체 필름에서 슈반세포의 부착과 증식 거동 비교평가)

  • Ko, Hyun Ah;Jang, Ji Eun;Kim, Hyeongseok;Park, Chan Hum;Kwon, Soon Yong;Chung, Jin Wha;Khang, Gilson
    • Polymer(Korea)
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    • v.38 no.2
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    • pp.164-170
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    • 2014
  • This study was designed to find an appropriate biomaterial to proliferate Schwann cell (SC). Poly(lactic-co-glycolic acid) (PLGA) films mixed with demineralized bone particle (DBP), small intestine submucosa (SIS), and silk were fabricated by a solvent casting method. Analysis of MTT, SEM and RT-PCR were performed to confirm adhesion and proliferation of SC. Contact angle of films was assayed for hydrophilicity of films. We confirmed that PLGA/DBP 20% film showed higher hydrophilicity, promoted adhesion and proliferation of SC than other films. It was concluded that PLGA/DBP film can be applied for the scaffold biomaterials for the regeneration of central nerve system.

The Conceptual Intersection between the Old and the New and the Transformation of the Traditional Knowledge System (신구(新舊) 관념의 교차와 전통 지식 체계의 변용)

  • Lee, Haenghoon
    • The Journal of Korean Philosophical History
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    • no.32
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    • pp.215-249
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    • 2011
  • This essay reflects on the modernity of Korea by examining the transformation of the traditional knowledge system from a historico-semantic perspective with its focus on the opposition and collision of the old and the new conception occurred in the early period(1890~1910) of the acceptance of the Western modern civilization. With scientific success, trick of reason, Christianity and evolutionary view of history, the Western modernity regarded itself as a peak of civilization and forced the non-Western societies into the world system in which they came to be considered as 'barbarism(野蠻)' or 'half-enlightened(半開).' The East Asian civilization, which had its own history for several centuries, became degraded as kind of delusion and old-fashioned customs from which it ought to free itself. The Western civilization presented itself as exemplary future which East Asian people should achieve, while East Asian past traditions came to be conceived as just unnecessary vestiges which it was better to wipe out. It can be said that East Asian modernization was established through the propagation and acceptance of the modern products of the Western civilization rather than through the preservation of its past experience and pursuit of the new at the same time. Accordingly, it is difficult to apply directly to East Asian societies Koselleck's hypothesis; while mapping out his Basic Concept of History, he assumed that, in the so-called 'age of saddle,' semantic struggle over concepts becomes active between the past experience and the horizon of expectation on the future, and concepts undergoes 'temporalization', 'democratization', 'ideologization', 'politicization.'The struggle over the old and new conceptions in Korea was most noticeable in the opposition of the Neo-Confucian scholars of Hwangseongsinmun and the theorists of civilization of Doknipsinmun. The opposition and struggle demanded the change of understanding in every field, but there was difference of opinion over the conception of the past traditional knowledge system. For the theorists of civilization, 'the old(舊)' was not just 'past' and 'old-fashioned' things, but rather an obstacle to the building of new civilization. On the other hand, it contained the possibility of regeneration(新) for the Neo-Confucian scholars; that is, they suggested finding a guide into tomorrow by taking lessons from the past. The traditional knowledge system lost their holy status of learning(聖學) in the process of its change into a 'new learning(新學),' and religion and religious tradition also weakened. The traditional knowledge system could change itself into modern learning by accepting scientific methodology which pursues objectivity and rationality. This transformation of the traditional knowledge system and 'the formation of the new learning from the old learning' was accompanied by the intersection between the old and new conceptions. It is necessary to pay attention to the role played by the concept of Sil(hak)(實學) or Practical Learning in the intersection of the old and new conceptions. Various modern media published before and after the 20th century show clearly the multi-layered development of the old and new conceptions, and it is noticeable that 'Sil(hak)' as conceptual frame of reference contributed to the transformation of the traditional knowledge system into the new learning. Although Silhak often designated, or was even considered equivalent to, the Western learning, Neo-Confucian scholars reinterpreted the concept of 'Silhak' which the theorists of civilization had monopolized until then, and opened the way to change the traditional knowledge system into the new learning. They re-appropriated the concept of Silhak, and enabled it to be invested with values, which were losing their own status due to the overwhelming scientific technology. With Japanese occupation of Korea by force, the attempt to transform the traditional knowledge system independently was obliged to reach its own limit, but its theory of 'making new learning from old one' can be considered to get over both the contradiction of Dondoseogi(東道西器: principle of preserving Eastern philosophy while accepting Western technology) and the de-subjectivity of the theory of civilization. While developing its own logic, the theory of Dongdoseogi was compelled to bring in the contradiction of considering the indivisible(道and 器) as divisible, though it tried to cope with the reality where the principle of morality and that of competition were opposed each other and the ideologies of 'evolution' and 'progress' prevailed. On the other hand, the theory of civilization was not free from the criticism that it brought about a crack in subjectivity due to its internalization of the West, cutting itself off from the traditional knowledge system.

A Study on Cu-based Catalysts for Oxygen Removal in Nitrogen Purification System (질소 정제 시스템의 산소 제거용 구리계 촉매 연구)

  • Oh, Seung Kyo;Seong, Minjun;Jeon, Jong-Ki
    • Clean Technology
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    • v.27 no.1
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    • pp.9-16
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    • 2021
  • Since the active matrix organic light-emitting diode (AMOLED) encapsulation process is very vulnerable to moisture and oxygen, high-purity nitrogen with minimal moisture and oxygen must be used. In this study, a copper-based catalyst used to remove oxygen from nitrogen in the AMOLED encapsulation process was optimized. Two-component and three-component catalysts composed of CuO, Al2O3, or ZnO were prepared through a co-precipitation method. The prepared catalysts were characterized by using BET, XRD, TPR, and XRF analysis. In order to verify the oxygen removal performance of the catalyst, several catalytic reactions were conducted in a fixed bed reactor, and the corresponding oxygen contents were measured through an oxygen analyzer. In addition, reusability of the catalysts was proven through repetitive regeneration. The properties and oxygen removal capacity of the catalysts prepared with CuO and Al2O3 ratios of 6 : 4, 7 : 3, and 8 : 2 were compared. The number of active sites of the catalyst with a ratio of CuO and Al2O3 of 8 : 2 was the highest among the 2-component catalysts. Moreover, the reducibility of the catalyst with a ratio of CuO and Al2O3 of 8 : 2 was the best as it had the highest CuO dispersion. As a result, the oxygen removal ability of the catalyst with a ratio of CuO and Al2O3 of 8 : 2 was the best among the 2-component catalysts. The best oxygen removal capacity was obtained when 2wt% of ZnO was added to the sub-optimized catalyst (i.e., CuO : Al2O3 = 8 : 2) probably due to its outstanding reducibility. Furthermore, the optimized catalyst kept its performance during a couple of regeneration tests.

A Study for Failure Examples of Emission Gas Recirculation and Air Control and Catalyzed Particulate Filter System in Diesel Engine Vehicle (디젤엔진 자동차의 EGR 및 공기 제어와 CPF 장치에 관련된 고장사례 고찰)

  • Lee, IL Kwon;Kook, Chang Ho;Ham, Sung Hoon;Lee, Young Suk;Youm, Kwang Wook;You, Chang Bae;Kim, Sung Mo;Lim, Ha Young;Ahn, Ho Cheol;Lee, Jeong Ho
    • Journal of the Korean Institute of Gas
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    • v.22 no.2
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    • pp.78-83
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    • 2018
  • The purpose of this paper is to study for failure examples of emission gas recirculation and air control and catalyzed particulate filter system in diesel engine vehicle. The first example, the researcher found the fact that the much engine oil came into the intake manifold causing diaphragm damage of EGR valve. The engine oil entered into combustion chamber of engine so that a car emit the polluted exhaust gas when driving. The second example, the researcher certified the sticking phenomenon of carbon and foreign substance with the throttle flap so that the exhaust fumes discharged exhaust port. The third example, the regeneration function don't activated to not detect the temperature of exhaust gas because of damage in the sensor. Thus, the researcher must meticulously manage his car not in order to take place the problem of environmental pollution.

PULP RESPONSE OF BEAGLE DOG TO DIRECT PULP CAPPING MATERIALS: HISTOLOGICAL STUDY (직접치수복조재에 따른 비글견 치수의 조직반응에 대한 연구)

  • Bae, Ji-Hyun;Kim, Young-Gyun;Yoon, Pil-Young;Cho, Byeong-Hoon;Choi, Yong-Hoon
    • Restorative Dentistry and Endodontics
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    • v.35 no.1
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    • pp.5-12
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    • 2010
  • The purpose of this study was to evaluate the pulp tissue reaction to direct pulp capping of mechanically exposed beagle dogs' pulp with several capping materials. A total of 36 teeth of 2 healthy beagle dongs were used. The mechanically exposed pulps were capped with one of the followings: (1) Mineral Trioxide Aggregate (MTA: $ProRoot^{(R)}$ MTA. Dentsply, Tulsa, USA), (2) Clearfil SE Bond (Dentin adhesive system: Kuraray, Osaka, Japan), (3) Ultra-Blend (Photo-polymerized Calcium hydroxide: Ultradent, South Jordan, USA), (4) Dycal (Quick setting Calcium hydroxide: LD Caulk Co., Milford, USA) at 7, 30, and 90 days before sacrificing. The cavities were restored with Z350 flowable composite resin (3M ESPE, St. Paul. MN, USA). After the beagle dogs were sacrificed, the extracted teeth were fixed, decalcified, prepared for histological examination and stained with HE stain. The pulpal tissue responses to direct pulp capping materials were assessed. In MTA calcium hydroxide, and photo-polymerized calcium hydroxide groups, initial mild inflammatory cell infiltration, newly formed odontoblast-like cell layer and hard tissue bridge formation were observed. Compared with dentin adhesive system, these materials were biocompatible and good for pulp tissue regeneration. In dentin adhesive system group, severe inflammatory cell infiltration, pulp tissue degeneration and pulp tissue necrosis were observed. It seemed evident that application of dentin adhesive system in direct pulp capping of beagle dog teeth cannot lead to acceptable repair of the pulp tissue with dentine bridge formation.

Network Planning on the Open Spaces in Geumho-dong, Seoul (서울 금호동 오픈스페이스 네트워크 계획)

  • Kang, Yon-Ju;Pae, Jeong-Hann
    • Journal of the Korean Institute of Landscape Architecture
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    • v.40 no.5
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    • pp.51-62
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    • 2012
  • Geumho-dong, Seoul, a redeveloped residential area, is located in the foothills of Mt. Eungbong. The geographical undulation, the composition of a large apartment complex, and the partial implementation of the redevelopment project have caused the severe physical and social disconnections in this area. In order to recover functioning in the disconnected community, this study pays attention to the regeneration of the open spaces as an everyday place and in the form a network system among those open spaces. Various types of the open spaces are classified into points or faces, 'bases' and linear 'paths' analyze the network status. More than half of the open space have connecting-distance of 500m or more. Furthermore, many areas are not even included in the service-area of the open spaces. Analysis of the connectivity and integration value using the axial map has carried out to check weak linkages and to choose the sections where additional bases are required. In addition, to improve the quality of the bases and the paths, a field investigation is conducted and problems are diagnosed. The network planning of the open spaces in Geumho-dong is established, ensuring the quality and quantity of bases and paths. The plan includes the construction of an additional major base in the central area and six secondary bases in other parts, and comes up with ways to improve the environment of underdeveloped secondary bases. In the neighborhood parks at Mt. Daehyun areas, the major path are added, and the environment of the paths is improved in certain areas. Because of the network planning, the connecting-distances between bases are reduced significantly, the connectivity and integration value of the area are increased, and the service areas of the open spaces cover the whole area properly. Although this study has some limitations such as the needs for the legal and institutional supports and difficulties of a quantitative indexing process, its significance lies in the suggestion of a more reasonable and practical plan for the overall network system by defining complex types of open spaces simply and clearly and by examining the organic relationships quantitatively and qualitatively.