• Title/Summary/Keyword: Co-culture technique

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Fire Regulation for Building Material of Organic Matter (유기물 건축내장재에 대한 화재법규)

  • Yoon, Kwan-Sun;Choi, Keun-Young
    • Proceedings of the SAREK Conference
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    • 2007.11a
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    • pp.136-142
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    • 2007
  • Due to the construction technique (or the construction culture) growth in and out of Korea, newly-developed products have been continuously released. Furthermore, all legislation and regulation that the World follows are gradually becoming unified. (e.g. FTA(Free Trade Agreement) with the United States was contracted, and inevitable FTA contraction with EU and China ), For this reason, it is considered that Korean fire regulation on building interior materials needs to be reviewed and compared with that of international standard so that it becomes legitimate and reasonable one. Especially, this reference is going to make a description of building interior materials' Flame retardant performance standard.

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Using automated container type culture system Hericium erinaceum mushroom production technology (컨테이너형 버섯식물공장시스템설계 및 노루궁뎅이버섯 생산)

  • Jo, Woo-Sik;Lee, Sung-Hak;Ryu, Song-Yi;Kang, Min-Gu;Kim, Woo-Hyun;Park, Chang-Min;Park, Who-Won
    • Journal of Mushroom
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    • v.14 no.4
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    • pp.249-252
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    • 2016
  • Timing, place, and the high-tech industry to overcome barriers to the introduct of the so-called "smart agriculture". The core factors of precision agriculture, including temperature, humidity, location information, and real summary information, are all significant for ICT controlling technique. The system is a four-season container allowing mushroom product, humidity, illumination, and factors such as carbon dioxide were regulated to create the optimal environment for mushroom cultivation. Hericium erinaceum (also called Lion's Mane Mushroom, Bearded Tooth Mushroom, or Bearded Tooth Fungus) is an edible and medicinal mushroom in the tooth fungus group. During approximately 40 days of incubation, 1.4 kg of oak sawdust was used as the test medium. The humidity, temperature, and $CO_2$ density were maintained at >85-95%, $18-20^{\circ}C$, and 700-1,000 ppm, respectively, in the container type culture system. The time for primordium formation was observed to be 4-7 days. The amount of fruitbody production was 74-95 g per bag. This technique will be used to establish a new community support program, in conjunction with international movement, domestic sales and exports are also planned.

Study on the Fashion Design Applying Pictures of Butterfly in the Korean Folk Paintings (조선 민화 나비를 응용한 의상 디자인 연구)

  • Lee, Jong-Min;Lee, Mi-Ryang
    • The Research Journal of the Costume Culture
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    • v.14 no.5
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    • pp.828-839
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    • 2006
  • These days, by the accelerated developments of science and industries and the menaces of war and terror, humans have come to have instinct to recur and nostalgia to nature and thereby many art works and designs with natural objectives have begun to be produced. Also the design in 21st century requires creative products based on cultural speciality of the countries. As the examples, we may easily find the images of the countries from visual expressions or products when we get in touch with the products of so called advanced countries in design i. e. U.S.A. Japan, Germany, France, Italy, countries of Scandinavian peninsular. This study, in such stream of the times, was to express our culture's own originality on clothing design with butterfly that is one of natural thing as the material but limited the boundary to realistic butterflies in folk drawings of the times of Chosun. As for the technique of expression, used digital textile printing for best expression of the fine lines, realistic shapes and peculiar colors of the butterflies in Chosun folk drawings and used diverse materials as materials for printing for diversity of the designs. Thereby this thesis is purposed to grope the products with superior competitiveness in the world market by presenting the realistic butterflies in Chosun folk drawings as motives with cultural value native to Korea and applying them to clothing designs.

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EFFECT OF SEVERAL RESTORATIVE MATERIALS ON ULRASTRUCTURAL CHANGES OF L929 CELLS USING TRANSMISSION ELECTRON MICROSCOPE (투과전자현미경을 이용한 수종의 수복재가 L292세포의 미세구조 변화에 미치는 영향)

  • Im, Mi-Kyung
    • Restorative Dentistry and Endodontics
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    • v.21 no.1
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    • pp.385-402
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    • 1996
  • Cytotoxicity of dental restorative materials using cell culture technique has been extensively studied by various quantitative assays. The aim of this study was to investigate the microstructural change of damaged L292 cells which could not observed with light microscope. Cytotoxic effect of ZOE, Prisma APH (Densply International Inc., U.S.A.), Clearfil FII(Kuraray Co., Japan), Fuji II(GC Co., Japan) and Fuji II LC(GC Co., Japan) on cultured L292 cells were observed. Irreversible cell damage and cytolysis were found in ZOE and Fuji II groups. In Clearfil FII, mild to moderate cell damage was observed. APH group showed variable cytotoxicity. Moderate cell damage was found in Fuji II LC group. Cytotoxic effect were as follows : A condensation of the chromatin occureds along or adjacent to the inner membrane of the nuclear envelops. The nuclear envelope remained resonably intact but the contents were partially or completely lost. The cell nucleus contains clusters of markedly electron-dense interchromatin granules. The rough endoplasmic reticulum were dilated. In some mitochondira, matrix was disoriented and fused cristae were discernible. Mitochondiral swelling and woolly appearance were recognized. Large vacuoles and autolysosmes were found in cytoplasm. Some breaks of the cytoplasmic membrane and even cytolysis could be seen in dying cells.

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Acinetobacter Isolates Growing with Carbon Monoxide (일산화탄소를 이용하여 성장하는 acinetobacter의 분리 및 동정)

  • 조진원;임현숙;김영민
    • Korean Journal of Microbiology
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    • v.23 no.1
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    • pp.1-8
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    • 1985
  • Three strains (JC1, JC2, and HY1) of aerobic carbon monoxide (CO)-utilizing Acinetobacter were isolated from soil through CO-enrichment culture technique. All of them were Gram-negative, nonmotile, and rod-shated but they were changed to spherical form at the end of logarithmic phase. They were resistant to penicillin and able to frow at $42^{\circ}C$. The guanine plus cytosine contents of the DNAs ranged from 43 to 44.5 mol%. Oxidase was not present in all cells. The colonies were smooth and whitish yellow. Heterotrophic growth occurred on several sugars, organic acids, amino acids, and alcohols. The doubling times under and atmosphere of 30% CO and 70% air at $30^{\circ}C$ were 19h, 25h, and 35h, respectively, for JC1, JC2, and HY1, JC1 was studied in more detail. The cells were grown optimally in a mineral medium (pH 6.8) under a gas mixture of 30% CO and 70% air at $30^{\circ}C$. Growth of the cells with CO did not depend on molybdenum. It was able to grow with 100 ppm of CO in air as a sole source of carbon and energy.

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Development of a safe culture technique for Agrocybe cylindracea (버들송이버섯 봉지재배 안정생산 기술)

  • Heo, Byong-Soo;Seo, Sang-Young;Choi, Kyu-Hwan;Choi, Young-Min;Kwon, Seog-Ju;Jang, Kab-Yeyl;Yoo, Young-Jin
    • Journal of Mushroom
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    • v.17 no.1
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    • pp.1-6
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    • 2019
  • Agrocybe cylindracea was cultured in a bag, in which sawdust culture medium (1 kg) is put in a plastic bag (PE), with poplar sawdust, rice bran, wheat bran, and dried bean curd refuse in the ratio of 70:10:10:10 (v/v). 2% of the culture medium was inoculated with the liquid starter of Agrocybe cylindracea, and this was incubated at $25^{\circ}C$. After incubation, the A. cylindracea was further cultured by cutting the top vinyl portion of the bag down to the level of the culture medium surface of the first inoculation part. The cut culture medium was placed in a growth room at $25^{\circ}C$, and pin-heading was induced under light irradiation at 99% humidity and 1,000 ppm $CO_$ level for 3days. When the grow the environment was controlled at 95% humidity and $21^{\circ}C$, the bending of the stem was less as compared to that when the cap of the bag had been removed. The number of effective fruiting bodies per bag increased by 140% (28.8), the quantity per bag increased by 29.5%, and 148.5 g A. cylindracea could be potentially harvested.

Antiviral Effects of Natural Products on the Inhibition of Hepatitis B Virus DNA Replication in 2.2.15 Cell Culture System

  • Nam, Kung-Woo;Chang, Il-Moo;Choi, Jae-Sue;Hwang, Ki-Jun;Mar, Woong-Chon
    • Natural Product Sciences
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    • v.2 no.2
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    • pp.130-136
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    • 1996
  • Evaluation of plant extracts that might inhibit hepatitis B virus (HBV) replication was performed to find potent anti-HBV agents. Eighty-five species of plants from forty-three families were tested for their anti-HBV activities using HBV-producing HepG2-derived 2.2.15 cells. The anti-HBV activity of plant extracts was measured by slot blot hybridization technique and cytotoxicity was determined by crystal violet staining procedure. All plants were extracted with methanol and the extracts were partitioned into n-hexane, ethyl acetate and aqueous layer. The ethyl acetate fractions of Rhus verniciflua $(stem:\;EC_{50},\;8.2{\mu}g/ml;\;CC_{50},\;9.4{\mu}g/ml)$, Gastrodia elata $(root:\;EC_{50},\;17.7{\mu}g/ml;\;CC_{50},\;>20{\mu}g/ml)$, Raphanus sativus $(seeds:\;EC_{50},\;17.3{\mu}g/ml;\;CC_{50},\;>20{\mu}g/ml)$, and Angelica gigas $(root:\;EC_{50},\;8.3{\mu}g/ml;\;CC_{50},\;15.6{\mu}g/ml)$ revealed the anti-HBV activity in 2.2.15 cell culture system and these fractions are under the process of further sequential fractionation by column chromatography to find the active principles against HBV.

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Status and Risk Factors of Strongyloides stercoralis Infection in Rural Communities of Xayaburi Province, Lao PDR

  • Senephansiri, Phasouk;Laummaunwai, Porntip;Laymanivong, Sakorn;Boonmar, Thidarut
    • Parasites, Hosts and Diseases
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    • v.55 no.5
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    • pp.569-573
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    • 2017
  • The present study was performed to reveal the current status and risk factors of Strongyloides stercoralis infections in the villages of Kenethao district, Xayaburi Province, Lao PDR. Fecal specimens were collected and examined for S. stercoralis using Koga-agar plate culture technique. Among 516 individuals, the prevalence of S. stercoralis and hookworm infection was 44.2% and 17.1%, respectively. Co-infection was detected in 13.2% of the cases. The prevalence did not significantly differ between males and females (P=0.193). However, the prevalence of S. stercoralis infection increased significantly with age (P=0.041). Of the risk factors examined, both performing farming activities (P=0.001) and walking barefoot when going outside of the house (P=0.003) showed significant correlations with S. stercoralis infections. Our results suggest that S. stercoralis is highly endemic in this area. The National Helminth Control Program of Lao PDR should take actions to control S. stercoralis infection. In addition, provision of health education about the benefits of wearing shoes would be important for reducing infection in the study area. Moreover, the application of high-sensitivity diagnostic approaches is needed to obtain the true impact of S. stercoralis infections in all rural communities in order to provide surveillance activities in Lao PDR.

Investigation of Coke Formation in Dry Methane Reforming over Nickel-based Monolithic Catalysts

  • Pornruangsakun, Pongsakorn;Tungkamani, Sabaithip;Ratana, Tanakorn;Phongaksorn, Monrudee;Sornchamni, Thana
    • International Journal of Advanced Culture Technology
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    • v.3 no.1
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    • pp.31-38
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    • 2015
  • Coking accumulations via dry methane reforming (DMR) over 10NAM monolithic catalyst and pelletized catalyst was investigated. 10NAM catalyst was synthesized and coated on a wall of monolithic reactor. Pelletized catalyst of 10NAM was also prepared for the comparison. Consequently, catalyst was characterized by BET, $H_2-TPR$ and $H_2-TPD$. The catalytic reaction was undergone at $600^{\circ}C$ under atmospheric pressure and $CH_4$ to $CO_2$ reactant ratio of 1:2. The coking formation over spent catalyst was then carried out in the hydrogen flow using temperature programmed technique (TPH). According to the results, DMR over 10NAM monolithic catalyst exhibits a minimized coking formation comparing to the use of pelletized catalyst. This could be attributed to a prominent heat transfer efficiency of the monolithic catalyst.

Analysis of Domestic Patent Information on Hydroponics Field (수경재배 분야의 국내 특허 정보 분석)

  • Yu, Sung-Oh;Bae, Jong-Hyang;Park, Yun-Jum;Cho, Ja-Yong;Jang, Hong-Gi;Heo, Buk-Gu
    • Journal of Bio-Environment Control
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    • v.16 no.1
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    • pp.13-20
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    • 2007
  • This study was conducted to collect the basic data fo the development ot hydroponic technique and the efficient promotion of water culture. We have also analysed the water culture associated domestic patent application informations searching for on-line internet site with an admission fee (http://www2.wips.co.kr) from the year 1983 to January, 2006. Fifty nine patent applications related to the hydroponics were searched for in this study. Main patent contents applied were as followed in the order of that nineteen applications about the hydroponic equipments by 32.2%, thirteen applications about the materials used in water culture by 22.0%, eleven applications about the composition of nutrient solution by 18.6%, nine applications about the cultural methods by 15.3%, and seven applications about the cultural media by 11.9%. The compositions of nutrient solution and the cultural methods intended for specific crops were increased in number following five applications about tomato plants and four applications about potatoes. Thirty three patents (55.9%) were mainly applied before the year 2000. Main patent contents were the compositions of nutrient solution before the year 2000 in contrast to the cultural methods and substrates after the year 2000.