Development of a safe culture technique for Agrocybe cylindracea

버들송이버섯 봉지재배 안정생산 기술

Abstract

Agrocybe cylindracea was cultured in a bag, in which sawdust culture medium (1 kg) is put in a plastic bag (PE), with poplar sawdust, rice bran, wheat bran, and dried bean curd refuse in the ratio of 70:10:10:10 (v/v). 2% of the culture medium was inoculated with the liquid starter of Agrocybe cylindracea, and this was incubated at $25^{\circ}C$. After incubation, the A. cylindracea was further cultured by cutting the top vinyl portion of the bag down to the level of the culture medium surface of the first inoculation part. The cut culture medium was placed in a growth room at $25^{\circ}C$, and pin-heading was induced under light irradiation at 99% humidity and 1,000 ppm $CO_$ level for 3days. When the grow the environment was controlled at 95% humidity and $21^{\circ}C$, the bending of the stem was less as compared to that when the cap of the bag had been removed. The number of effective fruiting bodies per bag increased by 140% (28.8), the quantity per bag increased by 29.5%, and 148.5 g A. cylindracea could be potentially harvested.

버들송이버섯을 봉지재배할 경우 비닐봉지(PE)에 포플러톱밥:미강:밀기울:건비지를 70:10:10:10 (v/v) 비율로 제조하여 1 kg씩 입봉하고 종균을 배지의 2%씩 접종 후 배양실 온도를 $25^{\circ}C$로 하여 배양하고 배양이 완료되면 최초 접종부위의 배지표면 높이까지 상부 비닐을 절개하여 재배를 하였다. 절개된 배지를 생육실에 입상하고 습도 99%, $CO_2$ 1,000 ppm, 1000 lx 조건에서 3일간 발이 유도 후 습도 95%, 온도 생육온도 $21^{\circ}C$ 조건으로 생육환경을 조절하면 봉지의 뚜껑을 제거한 처리구에 비해 대의 휘어짐은 적어지고 봉지 당 유효경수는 140% 증가한 28.8개, 봉지 당 수량은 29.5% 증가한 148.5 g을 수확 할 수 있다.

Fig. 1. Photos of mycelial growth and density of A. cylindracea cultivar ‘Cham’ (A, B, and C) and ‘JBAC15-1’ (D, E, and F) in different culture media (CM). CM of A and D : poplar sawdust : wheat bran : rice bran : soybean dry = 70 : 10 : 10 : 10 (v/v, %). CM of B and E : douglas fir sawdust : cotton-seed : flame grass pellet = 50 : 30 : 20 (v/v, %). CM of C and F : douglas fir sawdust : corn bran : wheat bran : flame grass pellet = 20 : 30 : 25 : 25 (v/v, %).

Fig. 2. Photos of fruit body of A. cylindracea cultivar ‘Cham’ (A and B) and ‘JBAC15-1’ (C) at the different culture media (CM). CM of A : poplar sawdust : wheat bran : rice bran : soybean dry = 70 : 10 : 10 : 10 → earlier developed of media. CM of B and C : douglas fir sawdust : corn bran : wheat bran : flame grass pellet = 20 : 30 : 25 : 25.

Fig. 3. Photos of growing fruit body of A. cylindracea cultivar ‘Cham’ at different culture temperature. A : 18℃. B : 21℃. C : 25℃.

Fig. 4. Photos of growing fruit body of A. cylindracea cultivar ‘Cham’ (A and C) and ‘JBAC15-1’ (B and D) after physical induction (PI). PI of A and B : removal of top surface on medium. PI of C and D : removal of cover on medium

Table 1. Mycelial growth and density of A. cylindracea in different culture media (v/v, %)

Table 2. Characteristics of fruit body of A. cylindracea in different culture media

Table 3. Characteristics of fruit body of A. cylindracea after culture at different temperatures

Table 4. Characteristics of cultivation of A. cylindracea after culture at different temperatures

Table 5. Characteristics of fruit body of A. cylindracea after physical induction

Table 6. Characteristics of cultivation and yield of A. cylindracea after physical induction