• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2002년도 추계학술대회
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• pp.33-40
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• 2002

We, Tong Yang Moolsan Co. Ltd. (TYM) set up the mass-production system for virus-free seed garlic via tissue culture technique. TYM's tissue culture technique is called as 'Multiple shoot propagation technique'. This technique can lead mass propagation of genetically homogeneous seed garlic in a short period because of its highly proliferation rate of in vitro shoots ($15^{10}$ /year). TYM researchers applied the technique to some selected garlic cultivars with superior characteristics and carried out field test of productivity in the inside and outside of the country for several years. According to the yearly results of field test with virus-free seed garlic, we ascertained that virus-free seed garlic can produce the highly yield increase (max. above $50\%$) and also can enhance the product quality. Consequently, we estimated that TYM's seed garlic will contribute to farmers with increase of income and can elevate the national position of garlic market in the world for its competitive power of technical and production cost.

• 농업과학연구
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• 제45권4호
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• pp.715-723
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• 2018

The present study was done to investigate the effect of co-culturing muscle satellite cells (MSCs) and intramuscular preadipocytes (IPs) on the differentiation of adipocytes and muscle cells isolated from Korean native cattle. MSCs and IPs were single-cultured in 10% fetal bovine serum/Dulbecco's modified Eagles medium (FBS/DMEM) for 48 h followed by culturing in 5% FBS/DMEM as the growth media. Then, the growth media was replaced by differentiation media composed of 2% FBS/DMEM without any additives for the single- or co-culture of muscle cells and intramuscular adipocytes to induce the differentiation of both cell types. Cell differentiation was measured by morphological investigation and cytosolic enzyme analysis of glycerol-3-phosphate dehydrogenase (GPDH) for the adipocytes and creatine kinase (CK) for the muscle cells. In the morphological test, the presence of muscle cells did not stimulate adipocyte differentiation showing more differentiation of the adipocytes in the single-culture compared to the co-culture condition. However, the differentiation of muscle cells was promoted by adipocytes in the co-culture. The results of the enzymatic analysis were highly associated with the morphological results with a statistically higher GPDH activity (p < 0.05) appearing in the single-culture than in the co-culture, whereas the opposite was true for the CK activity of the muscle cells (p < 0.05). By manipulating in vivo the milieu using a co-culture, we could detect the difference in the rate of cell differentiation and suggest that a co-culture system is a more reliable and precise technique compared to a single-culture. Further studies on various co-culture trials including supplementation of differentiating substances, gene expression analysis, etc. should be done to obtain practical and fundamental data.

• 한국식물생명공학회:학술대회논문집
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• 한국식물생명공학회 2002년도 춘계학술대회
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• pp.33-40
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• 2002

동양물산 중앙기술연구소에서 개발한 다신초 (multiple shoot) 증식기술을 이용하면 1회 계대배양으로 신초의 수가 약 15배 증가하고 1년에 10회의 계대배양이 가능하므로 계산상 1개의 신초로부터 5,700억개 (1510)의 신초 생산ㅇ 가능하므로 유전적으로 동질의 씨마늘을 단기간에 대량증식시키는 것이 가능하다 이러한 기술을 우수한 형질을 가진 마늘종에 적용하여 현재 국내${\cdot}$외 여러 지역에서 적응성 및 안정성 생산성 검정 실험을 수행하여 산업화 진입을 준비하는 단계에 도달하였다. 수년에 걸친 검정 시험 결과 조직배양 씨마늘을 이용할 경우 최대 50% 이상의 증수효과는 물론 상품성이 증대되는 효과도 확인하여 우리 나라 마늘산업의 국제 경쟁력을 향상시키고 능민 소득 증대에 크게 기여할 수 있을 것으로 판단된다. 또한 조직배양을 통해 대량생산된 무병주 씨마늘을 농가 위탁, 계약재배 등을 통해 증식하여 실수요 농가에 보급하는 체계를 갖추게 된다면 타 주요작물에 비해 상대적으로 미진한 종구 보급체계도 확립될 수 있을 것이다. 더불어 국제경쟁력을 갖춘 기술력, 원가 등을 기반으로 국내뿐만 아니라 일본, 미국 등 국외지역에도 공급지역을 확대할 수 있을 것으로 기대된다.

• Journal of Plant Biotechnology
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• 제29권3호
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• pp.171-177
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• 2002

동양물산 중앙기술연구소에서 개발한 다신초 (multiple shoot)증식기술을 이용하면 1회 계대배양으로 신초의 수가 약 15배 증가하고 1년에 10회의 계대배양이 가능하므로 계산상 1개의 신초로부터 5,700억 개 (1510)의 신초 생산이 가능하므로 유전적으로 동질의 씨마늘을 단기간에 대량증식시키는 것이 가능하다. 이러한 기술을 우수한 형질을 가진 마늘종에 적용하여 현재 국내·외 여러 지역에서 적응성 및 안정적 생산성 검정 시험을 수행하여 산업화 진입을 준비하는 단계에까지 도달하였다. 수년에 걸친 검정시험 결과 조직배양 씨마늘을 이용할 경우 최대 50%이상의 증수효과는 물론 상품성이 증대되는 효과도 확인하여 우리나라 마늘산업의 국제경쟁력을 향상시키고 농민 소득 증대에 크게 기여할 수 있을 것으로 판단된다. 또한 조직배양을 통해 대량생산된 무병주 씨마늘을 농가 위탁, 계약재배 등을 통해 증식하여 실수요 농가에 보급하는 체계를 갖추게 된다면 타 주요작물에 비해 상대적으로 미진한 종구 보급체계도 확립될 수 있을 것이다. 더불어 국제경쟁력을 갖춘 기술력, 원가 등을 기반으로 국내뿐만 아니라 일본, 미국 등 국외지역에도 공급지역을 확대할 수 있을 것으로 기대된다.

• 대한화장품학회지
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• 제30권4호
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• pp.463-470
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• 2004

인공피부배양물(DA-3711)은 동아제약의 인공피부배양기술을 활용하여 개발되었으며, 노화된 피부상태를 개선시켜 줄 수 있는 천연의 세포외기질 단백질을 비롯하여 인체성장인자 등의 영양성분을 함유하고 있다 DA-3711의 항노화 효과는 in vitro와 in vivo에서 규명되었으며, 또한 인체 효능 연구 결과에서도 피부탄력을 개선시키고 주름을 감소시키는데 매우 효과적이었다. 즉, DA-3711을 이용한 새로운 주름개선제는 피부재생을 촉진시켜 항노화 및 주름개선 효능을 나타낸다.

• 한국수정란이식학회지
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• 제29권3호
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• pp.207-219
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• 2014

Three-dimensional (3D) culture system is useful technique for study of in vivo environment and it was used various experiments. This study was investigated to establish of embryo co-culture system and changes of PAs activity in 3D cultured endometrial cells of pigs. In results, growth of stromal cells into gel matrix were detected only with endometrial and myometrial cells. The most rapid growth of stromal cells were confirmed in $2.5{\times}10^5cells/ml$ and gel matrix containing 15% FBS. Expression of urokinase-PA (uPA) after treatment of hCG (0.5, 1.0, 1.5 and 2.0 IU/ml) were higher than without hCG, but, there are not significant difference among the treatment. On the other hand, expression of uPA after treatment of $IL-1{\beta}$ (0.1, 1, 10 and 100 ng/ml) were higher than without $IL-1{\beta}$, but, there are not significant difference. Expression of uPA after treatment of estrogen (0.2, 2, 20 and 200 ng/ml) were not difference, but PA activity was significantly decreased (p<0.05). Blastocyst was producing in PZM-3 medium containing FBS and endometrial cells were grown in PZM-3 medium. When embryos development with cultured endometrial cells, cleavage rates were not significant difference and blastocyst were not produced in co-culture with stromal cells and 3D culture system. 3D culture system had similar activity to in vivo tissue and these features are very useful for study of in vivo physiology. Nevertheless 3D culture system was not proper in embryo co-culture system. Therefore, we suggest that 3D culture system with embryo co-culture need continuous research.

• KSBB Journal
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• 제22권3호
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• pp.146-150
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• 2007

Poly (3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] 미립구를 용매증발법으로 제조하였다. 3.9 mol% 4HB 조성의 P(3HB-co-4HB)를 Ralstonia eutropha의 유가식 배양으로부터 합성하였다. 계면활성제의 농도 및 종류(Tween 80, sodium dodecylsulfate, polyvinyl alcohol), 분산안정제 (Acacia)의 첨가, 고분자 및 모델 약물 (bovine serum albumin)의 농도 등이 미립구 입자 크기에 미치는 효과 및 in vitro 약물 방출 특성을 조사하였다. 평균 입자크기는 분산 안정제 첨가시 감소하였으며, 계면활성제, 약물 및 고분자의 농도가 증가할수록 증가하였다. 약물 방출량은 입자 크기가 감소할수록 증가하였다.

• 지역사회간호학회지
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• 제9권2호
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• pp.441-457
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• 1998

The purpose of this study is to provide fundamental information for the development of new sex education image media through the analysis of those tools produced by Planned Parenthood Federation of Korea. Twenty three video tapes related to the sex education were analysed. The data were sampled on the basis of the sex education instructions for primary and secondary schools published by the Seoul Office of Education in 1996 and the list of sex education information composed by the Planned Parenthood Federation of Korea with the following requirements. 1) The education materials are oriented to the middle and high school students as well a juveniles and are being used in public health centers and schools. 2) The education materials are not confined to the single subject such as AIDS or contraception. Because this study is focused on the analysis of image media, an analysis method widely and effectively used in public media study was chosen. The frequency analysis and percentage treatment were done by an SAS program. The results of the study are as follows : 1. An analysis of the material structure type showed that the frequency of the following subjects in 15 video tapes, composed as drama and cartoon, had this decreasing orde : sex behavior, sex relation ships, sex health, human development, personal technique, and society and culture. 2. The eight tapes of explanation and counseling showed a different sequence from that of the drama and cartoon material, primarily treating human development with 5419 seconds (47.9%) of type. The following were also considered in decreasing order: at 2000seconds (17.6%) sex behavior, at 1366 seconds (12.1%) sex health, at 1240 seconds (11.0%) society and culture, at 667 seconds (6.0%) relation ships, and at 611 seconds (5.4%) personal technique. 3. In a thematic analysis those education materials dealing with human development show this frequency sequence: anatomic physiology, reproduction, and adolescence. In relation, ships the sequence is dates, love, marriage and vows, and family. For personal technique, it is expressed as asking for help, and an empnasis on moral values. For sex behavior, they focus on abstinence, the co-responsibility of sex behavior, the sexual response of the human, and sexual nightmares. For the sex health area, the focus is contraception with abortion and reproductive health following that. In society and culture diversity is considered. 4. An analysis of the differences in production and content between 1980s and 1990s was also done. For materials produced in the 1980s the frequency of the subjects follows this order: sex behavior, human development, relation ships, sex health, personal technique, and society and culture with sub subjects such as abstinence, dating, adolescence, anatomic physiology, reproduction, calls for help, and the sexual response of a human being. For materials produced in the 1990s, highest frequency was sex behavior, the same as the 1980s. The rest of the material follows this decreasing order: relationships, human development, sex health, society and culture, and personal technique with sub subjects such as dating, co-resposibility in sex behavior, abstinence, and love frequently considered.

• 대한설비공학회:학술대회논문집
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• 대한설비공학회 2008년도 동계학술발표대회 논문집
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• pp.429-433
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• 2008

Due to the construction technique (or the construction culture) growth in and out of Korea, newly-developed products have been continuously released. Furthermore, all legislation and regulation that the World follows are gradually becoming unified. (e.g. FTA(Free Trade Agreement) with the United States was contracted, and inevitable FTA contraction with EU and China), For this reason, it is considered that Korean fire regulation on building interior materials needs to be reviewed and compared with that of international standard so that it becomes legitimate and reasonable one.

• Journal of Animal Science and Technology
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• 제54권2호
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• pp.103-109
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• 2012

본 연구는 기존 단독배양 위주로 이루어져온 세포배양 연구의 방법학적 한계의 극복과 대안을 제시하고자 지방과 근육세포주의 단독 및 공동배양에서 배양기법에 따른 지방 및 근육세포의 분화에 미치는 영향을 비교 조사하고자 실시하였다. 3T3-L1 (지방세포) 및 L6 (근육세포) 세포주는 성장배지인 10% FBS/DMEM (1% Pen-Strep solution 및 0.1% Fungizone 첨가) 하에서 48h 동안 단독배양 후 5% FBS/DMEM에서 배양하였다. 분화를 위한 단독 및 공동배양에서는 지방 및 근육세포 모두 분화유도물질 없이 2% FBS/DMEM으로 배양하였고, 공동배양에서는 $0.4{\mu}m$ insert membrane을 사용하여 6 well plate 하단에 L6 cell을, 상단에는 3T3-L1 cell을 공생시켰다. 지방 및 근육세포 분화정도 측정은 세포별 형태학적 측정과 glycerol-3-phosphate dehydrogenase (GPDH) 및 creatine kinase (CK) 분석을 통해 조사되었다. 형태학적으로 볼때 3T3-L1 세포주는 공동배양보다 단독배양 시 분화가 더욱 잘 일어났고 L6 세포주의 경우 역으로 같았다. 세포물질 분석에서는 분화배지 처리일(day 0)과 비교해 단독 및 공동배양 모두 지방세포 내 GPDH의 활성도가 유의적으로(P<0.05) 증가했음을 확인할 수 있었고 단독배양이 공동배양보다 유의적으로(P<0.05) 높은 수준의 GPDH 활성도를 보였다. L6 역시 마찬가지로 분화배지 처리일에 비하여 단독 및 공동배양 모두 CK 활성도가 유의적으로(P<0.05) 높았고, CK 활성도가 공동배양에서 유의적으로(P<0.05) 높게 나타남을 확인할 수 있었다. 이러한 결과는 기존 연구에서 이용된 단독 배양을 통한 세포 분화 결과 등은 생체와 비교 시 방법학적 한계로 인해 실제 생체 내에서는 그 분화정도가 매우 다를 것으로 생각되며, 이것은 앞으로 정확한 세포배양 결과 확보를 위해서는 단독배양보다는 공동배양기법을 사용해야 함을 의미한다. 향후 다양한 조건과 분화조절 물질들의 첨가를 통한 추가적인 공동배양실험이나 지방분화관련 분자생물학적 물질분석 등 다양한 실험 수행 시 보다 현실적이고 대량의 기초자료 확보가 가능할 것으로 판단된다.